Restriction map for pF1KA0188
 (Linear) (Six-base) MAPSORT of: KIAA0188.seq  Check: 1834  from: 1  to: 2670



KIAA0188.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AccI GT'mk_AC

Cuts at:      0    1190    2537    2670 
   Size:       1190    1347     133

  Fragments arranged by size: 

                1347    1190     133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     926    1241    2558    2583    2670 
   Size:        520     406     315    1317      25      87

  Fragments arranged by size: 

                1317     520     406     315      87      25
 
AflIII A'CryG_T

Cuts at:      0     539    2670 
   Size:        539    2131
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2254    2670 
   Size:       2254     416
 
ApaI G_GGCC'C

Cuts at:      0    2252    2670 
   Size:       2252     418
 
ApoI r'AATT_y

Cuts at:      0     899    2152    2670 
   Size:        899    1253     518

  Fragments arranged by size: 

                1253     899     518
 
AvaI C'yCGr_G

Cuts at:      0     179    1332    2670 
   Size:        179    1153    1338

  Fragments arranged by size: 

                1338    1153     179
 
BamHI G'GATC_C

Cuts at:      0    1215    2670 
   Size:       1215    1455
 
BanI G'GyrC_C

Cuts at:      0    1987    2670 
   Size:       1987     683
 
BanII G_rGCy'C

Cuts at:      0      53     370     562    1091    1296    2252    2670 
   Size:         53     317     192     529     205     956     418

  Fragments arranged by size: 

                 956     529     418     317     205     192      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     842    1043    1882    2670 
   Size:        842     201     839     788

  Fragments arranged by size: 

                 842     839     788     201
 
BbvCI CC'TCA_GC

Cuts at:      0    1372    1414    2664    2670 
   Size:       1372      42    1250       6

  Fragments arranged by size: 

                1372    1250      42       6
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2586    2670 
   Size:       2586      84
 
BclI T'GATC_A

Cuts at:      0     282    2670 
   Size:        282    2388
 
BfrBI ATG'CAT

Cuts at:      0    1444    2670 
   Size:       1444    1226
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2247    2670 
   Size:       2247     423
 
BlpI GC'TnA_GC

Cuts at:      0    1762    2117    2670 
   Size:       1762     355     553

  Fragments arranged by size: 

                1762     553     355
 
Bme1580I G_kGCm'C

Cuts at:      0     987    1990    2233    2252    2670 
   Size:        987    1003     243      19     418

  Fragments arranged by size: 

                1003     987     418     243      19
 
BmgBI CAC'GTC

Cuts at:      0     147    1938    2670 
   Size:        147    1791     732

  Fragments arranged by size: 

                1791     732     147
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     713    2016    2223    2670 
   Size:        713    1303     207     447

  Fragments arranged by size: 

                1303     713     447     207
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     576    1377    2670 
   Size:        576     801    1293

  Fragments arranged by size: 

                1293     801     576
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604    1063    1471    1782    2670 
   Size:        604     459     408     311     888

  Fragments arranged by size: 

                 888     604     459     408     311
 
Bpu10I CC'TnA_GC

Cuts at:      0    1372    1414    2664    2670 
   Size:       1372      42    1250       6

  Fragments arranged by size: 

                1372    1250      42       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1039    2670 
   Size:       1039    1631
 
BsaAI yAC'GTr

Cuts at:      0       9    1973    2670 
   Size:          9    1964     697

  Fragments arranged by size: 

                1964     697       9
 
BsaBI GATnn'nnATC

Cuts at:      0     281    2670 
   Size:        281    2389
 
BsaHI Gr'CG_yC

Cuts at:      0     433    1187    2670 
   Size:        433     754    1483

  Fragments arranged by size: 

                1483     754     433
 
BsaWI w'CCGG_w

Cuts at:      0     727    1261    1741    2670 
   Size:        727     534     480     929

  Fragments arranged by size: 

                 929     727     534     480
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1122    1152    1550    1580    2465    2495    2670 
   Size:       1122      30     398      30     885      30     175

  Fragments arranged by size: 

                1122     885     398     175      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     489    1099    1555    1822    2670 
   Size:        489     610     456     267     848

  Fragments arranged by size: 

                 848     610     489     456     267
 
BseYI C'CCAG_C

Cuts at:      0     397     424    2266    2670 
   Size:        397      27    1842     404

  Fragments arranged by size: 

                1842     404     397      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2194    2272    2670 
   Size:       2194      78     398

  Fragments arranged by size: 

                2194     398      78
 
BsiHKAI G_wGCw'C

Cuts at:      0      53     562    1091    1360    2670 
   Size:         53     509     529     269    1310

  Fragments arranged by size: 

                1310     529     509     269      53
 
BsmI GAATG_Cn'

Cuts at:      0     355    1444    2670 
   Size:        355    1089    1226

  Fragments arranged by size: 

                1226    1089     355
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     424    2670 
   Size:        424    2246
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     370     562     987    1091    1296    1360    1990 
   Size:         53     317     192     425     104     205      64     630

Cuts at:   1990    2233    2252    2670 
   Size:        243      19     418

  Fragments arranged by size: 

                 630     425     418     317     243     205     192     104
                  64      53      19
 
BspEI T'CCGG_A

Cuts at:      0    1261    2670 
   Size:       1261    1409
 
BspHI T'CATG_A

Cuts at:      0    1622    2670 
   Size:       1622    1048
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2216    2670 
   Size:       2216     454
 
BsrDI GCAATG_nn'

Cuts at:      0    1580    2670 
   Size:       1580    1090
 
BsrFI r'CCGG_y

Cuts at:      0      22    2670 
   Size:         22    2648
 
BssHII G'CGCG_C

Cuts at:      0    1812    2670 
   Size:       1812     858
 
BssSI C'ACGA_G

Cuts at:      0    1513    2670 
   Size:       1513    1157
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1276    2670 
   Size:       1276    1394
 
BstEII G'GTnAC_C

Cuts at:      0     707    2670 
   Size:        707    1963
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     118    1515    1938    2184    2670 
   Size:        118    1397     423     246     486

  Fragments arranged by size: 

                1397     486     423     246     118
 
BstYI r'GATC_y

Cuts at:      0    1215    1254    2670 
   Size:       1215      39    1416

  Fragments arranged by size: 

                1416    1215      39
 
Bsu36I CC'TnA_GG

Cuts at:      0     638    1050    2670 
   Size:        638     412    1620

  Fragments arranged by size: 

                1620     638     412
 
BtgI C'CryG_G

Cuts at:      0     572     779    2670 
   Size:        572     207    1891

  Fragments arranged by size: 

                1891     572     207
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1374    2097    2670 
   Size:       1374     723     573

  Fragments arranged by size: 

                1374     723     573
 
BtsI GCAGTG_nn'

Cuts at:      0    1720    2626    2670 
   Size:       1720     906      44

  Fragments arranged by size: 

                1720     906      44
 
ClaI AT'CG_AT

Cuts at:      0    1499    2670 
   Size:       1499    1171
 
DraI TTT'AAA

Cuts at:      0    2325    2670 
   Size:       2325     345
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     100    2670 
   Size:        100    2570
 
EaeI y'GGCC_r

Cuts at:      0      24     308    1769    2067    2670 
   Size:         24     284    1461     298     603

  Fragments arranged by size: 

                1461     603     298     284      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     454     496     605    1856    2281    2514    2670 
   Size:        454      42     109    1251     425     233     156

  Fragments arranged by size: 

                1251     454     425     233     156     109      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      97    2203    2670 
   Size:         97    2106     467

  Fragments arranged by size: 

                2106     467      97
 
EcoICRI GAG'CTC

Cuts at:      0      51     560    1089    2670 
   Size:         51     509     529    1581

  Fragments arranged by size: 

                1581     529     509      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     604     926    1063    1241    1471    1782    2558 
   Size:        520      84     322     137     178     230     311     776

Cuts at:   2558    2583    2670 
   Size:         25      87

  Fragments arranged by size: 

                 776     520     322     311     230     178     137      87
                  84      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2089    2670 
   Size:       2089     581
 
EcoO109I rG'GnC_Cy

Cuts at:      0     168    1377    1410    2248    2249    2670 
   Size:        168    1209      33     838       1     421

  Fragments arranged by size: 

                1209     838     421     168      33       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2588    2670 
   Size:       2588      82
 
HaeII r_GCGC'y

Cuts at:      0    1066    2670 
   Size:       1066    1604
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453     654     686    1122    1154    2670 
   Size:        421      32     201      32     436      32    1516

  Fragments arranged by size: 

                1516     436     421     201      32      32      32
 
HincII GTy'rAC

Cuts at:      0     195    2220    2463    2538    2670 
   Size:        195    2025     243      75     132

  Fragments arranged by size: 

                2025     243     195     132      75
 
HindIII A'AGCT_T

Cuts at:      0    1908    2670 
   Size:       1908     762
 
MscI TGG'CCA

Cuts at:      0     310    1771    2069    2670 
   Size:        310    1461     298     601

  Fragments arranged by size: 

                1461     601     310     298
 
MslI CAynn'nnrTG

Cuts at:      0     553    1175    1210    2182    2670 
   Size:        553     622      35     972     488

  Fragments arranged by size: 

                 972     622     553     488      35
 
MspA1I CmG'CkG

Cuts at:      0     357     489    1819    1979    2407    2670 
   Size:        357     132    1330     160     428     263

  Fragments arranged by size: 

                1330     428     357     263     160     132
 
NaeI GCC'GGC

Cuts at:      0      24    2670 
   Size:         24    2646
 
NcoI C'CATG_G

Cuts at:      0     572    2670 
   Size:        572    2098
 
NdeI CA'TA_TG

Cuts at:      0     230    2670 
   Size:        230    2440
 
NgoMIV G'CCGG_C

Cuts at:      0      22    2670 
   Size:         22    2648
 
NsiI A_TGCA'T

Cuts at:      0    1446    2670 
   Size:       1446    1224
 
NspI r_CATG'y

Cuts at:      0     543    1279    2494    2670 
   Size:        543     736    1215     176

  Fragments arranged by size: 

                1215     736     543     176
 
PasI CC'CwG_GG

Cuts at:      0    2244    2670 
   Size:       2244     426
 
PciI A'CATG_T

Cuts at:      0     539    2670 
   Size:        539    2131
 
PfoI T'CCnGG_A

Cuts at:      0    1144    1448    2670 
   Size:       1144     304    1222

  Fragments arranged by size: 

                1222    1144     304
 
PmlI CAC'GTG

Cuts at:      0    1973    2670 
   Size:       1973     697
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     526     558    2670 
   Size:        526      32    2112

  Fragments arranged by size: 

                2112     526      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     168    1377    2670 
   Size:        168    1209    1293

  Fragments arranged by size: 

                1293    1209     168
 
PsiI TTA'TAA

Cuts at:      0    1541    2670 
   Size:       1541    1129
 
PspOMI G'GGCC_C

Cuts at:      0    2248    2670 
   Size:       2248     422
 
PvuII CAG'CTG

Cuts at:      0     357     489    1819    2407    2670 
   Size:        357     132    1330     588     263

  Fragments arranged by size: 

                1330     588     357     263     132
 
SacI G_AGCT'C

Cuts at:      0      53     562    1091    2670 
   Size:         53     509     529    1579

  Fragments arranged by size: 

                1579     529     509      53
 
SalI G'TCGA_C

Cuts at:      0    2536    2670 
   Size:       2536     134
 
SfcI C'TryA_G

Cuts at:      0     449    2670 
   Size:        449    2221
 
SspI AAT'ATT

Cuts at:      0    1537    2453    2670 
   Size:       1537     916     217

  Fragments arranged by size: 

                1537     916     217
 
StuI AGG'CCT

Cuts at:      0     389    1582    2670 
   Size:        389    1193    1088

  Fragments arranged by size: 

                1193    1088     389
 
StyI C'CwwG_G

Cuts at:      0     572    1965    2085    2670 
   Size:        572    1393     120     585

  Fragments arranged by size: 

                1393     585     572     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     726    2670 
   Size:        726    1944
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     688     705     720     737    1550    1582    2670 
   Size:        688      17      15      17     813      32    1088

  Fragments arranged by size: 

                1088     813     688      32      17      17      15
 
Tth111I GACn'n_nGTC

Cuts at:      0     435    1186    2670 
   Size:        435     751    1484

  Fragments arranged by size: 

                1484     751     435
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     151     579     812    2670 
   Size:        151     428     233    1858

  Fragments arranged by size: 

                1858     428     233     151
 
XmnI GAAnn'nnTTC

Cuts at:      0    2568    2670 
   Size:       2568     102
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
   HincII  HindIII     MscI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NsiI     NspI     PasI     PciI     PfoI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PvuII     SacI     SalI     SfcI     SspI
     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BglII     BmtI    BpuEI    BsiEI    BsiWI
    BsrBI    BsrGI    BstBI  BstZ17I    CspCI   DraIII     EagI    EcoRI
    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NarI     NheI     NotI     NruI     PacI    PflMI
     PmeI    PshAI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI
     TatI     XbaI     XhoI     XmaI     ZraI
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