Restriction map for pF1KA0101
 (Linear) (Six-base) MAPSORT of: KIAA0101.seq  Check: 2296  from: 1  to: 333



KIAA0101.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     333 
   Size:        269      64
 
ApoI r'AATT_y

Cuts at:      0     199     333 
   Size:        199     134
 
AvaI C'yCGr_G

Cuts at:      0      56     333 
   Size:         56     277
 
BanII G_rGCy'C

Cuts at:      0      63     333 
   Size:         63     270
 
BclI T'GATC_A

Cuts at:      0     309     333 
   Size:        309      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     270     333 
   Size:        270      63
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     333 
   Size:         63     270
 
BtsI GCAGTG_nn'

Cuts at:      0     270     333 
   Size:        270      63
 
EarI CTCTTCn'nnn_

Cuts at:      0     245     333 
   Size:        245      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     333 
   Size:        269      64
 
EcoRI G'AATT_C

Cuts at:      0     199     333 
   Size:        199     134
 
MslI CAynn'nnrTG

Cuts at:      0     317     333 
   Size:        317      16
 
NspI r_CATG'y

Cuts at:      0     296     333 
   Size:        296      37
 
PspXI vC'TCGA_Gb

Cuts at:      0      56     333 
   Size:         56     277
 
SmlI C'TyrA_G

Cuts at:      0      56     333 
   Size:         56     277
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     176     333 
   Size:        176     157
 
XhoI C'TCGA_G

Cuts at:      0      56     333 
   Size:         56     277
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BanII     BclI   Bpu10I Bsp1286I     BtsI
     EarI  Eco57MI    EcoRI     MslI     NspI    PspXI     SmlI     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XmaI     XmnI     ZraI
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