Restriction map for pF1KA0059
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AccII       AccIII      AclI        AcsI        AflII       
AgeI        AhdI        Aor51HI     ApaI        ApoI        
AscI        AspEI       AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     CelII       ClaI        CpoI        
DraIII      DrdI        Eam1105I    Eco47III    EcoRI       
EcoT22I     FbaI        FseI        FspI        HpaI        
KasI        KspI        MamI        MluI        MroI        
MvnI        NarI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
TaqI        TthHB8I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII        1613  GACGTC               GACGT^C
Acc65I       1133  GGTACC               G^GTACC
AccI          946  GTAGAC               GT^[AC][GT]AC
AcyI         1613  GACGTC               G[GA]^CG[CT]C
AflIII       1946  ACATGT               A^C[GA][CT]GT
AlwNI         529  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           73  CCTCAGG              CC^T[ACGT]AGG
AseI         1638  ATTAAT               AT^TAAT
AsnI         1638  ATTAAT               AT^TAAT
Asp718I      1133  GGTACC               G^GTACC
BalI         1899  TGGCCA               TGG^CCA
BbsI          840  GAAGAC               GAAGAC(2/6)
BpuAI         840  GAAGAC               GAAGAC(2/6)
BsaHI        1613  GACGTC               G[GA]^CG[CT]C
BsaI          401  GAGACC               GGTCTC(1/5)
BseRI        1928  GAGGAG               GAGGAG(10/8)
BsgI          255  CTGCAC               GTGCAG(16/14)
BsiEI        1278  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1199  TGTACA               T^GTACA
BspLU11I     1946  ACATGT               A^CATGT
BsrBI        1233  CCGCTC               CCGCTC(-3/-3)
BsrGI        1199  TGTACA               T^GTACA
BssSI        1170  CACGAG               CACGAG(-5/-1)
Bsu36I         73  CCTCAGG              CC^T[ACGT]AGG
DraI          571  TTTAAA               TTT^AAA
EagI         1278  CGGCCG               C^GGCCG
EclXI        1278  CGGCCG               C^GGCCG
Eco52I       1278  CGGCCG               C^GGCCG
Eco81I         73  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2013  CCTCAGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI         2120  GACGC                GACGC(5/10)
Hin1I        1613  GACGTC               G[GA]^CG[CT]C
HincII        437  GTCAAC               GT[CT]^[GA]AC
HindII        437  GTCAAC               GT[CT]^[GA]AC
HindIII      1645  AAGCTT               A^AGCTT
KpnI         1133  GGTACC               GGTAC^C
MluNI        1899  TGGCCA               TGG^CCA
MscI         1899  TGGCCA               TGG^CCA
NaeI          499  GCCGGC               GCC^GGC
NdeI         1970  CATATG               CA^TATG
NgoMIV        499  GCCGGC               G^CCGGC
PflMI         806  CCACCGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1638  ATTAAT               AT^TAAT
PvuII         738  CAGCTG               CAG^CTG
SacI           25  GAGCTC               GAGCT^C
SapI         1630  GAAGAGC              GCTCTTC(1/4)
SexAI         921  ACCAGGT              A^CC[AT]GGT
SphI         1410  GCATGC               GCATG^C
SspBI        1199  TGTACA               T^GTACA
StuI         1656  AGGCCT               AGG^CCT
Van91I        806  CCACCGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          651  CCAGGGACTCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Alw44I        446  GTGCAC               G^TGCAC
              743  GTGCAC              
ApaLI         446  GTGCAC               G^TGCAC
              743  GTGCAC              
Asp700I       145  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2059  GAAGTGTTTC          
AspI          403  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1104  GACTTTGTC           
BanI         1133  GGTACC               G^G[CT][GA]CC
             1482  GGCACC              
BanII          25  GAGCTC               G[GA]GC[CT]^C
             1179  GGGCTC              
BfaI          893  CTAG                 C^TAG
             1577  CTAG                
BglII        1023  AGATCT               A^GATCT
             1573  AGATCT              
BspMI         190  ACCTGC               ACCTGC(4/8)
              715  GCAGGT              
BsrDI        1262  GCAATG               GCAATG(2/0)
             1912  GCAATG              
BstEII       1986  GGTGACC              G^GT[ACGT]ACC
             2105  GGTTACC             
BstPI        1986  GGTGACC              G^GT[ACGT]ACC
             2105  GGTTACC             
DsaI           47  CCATGG               C^C[GA][CT]GG
              294  CCATGG              
EcoO65I      1986  GGTGACC              G^GT[ACGT]ACC
             2105  GGTTACC             
EcoRV         514  GATATC               GAT^ATC
             1447  GATATC              
MaeI          893  CTAG                 C^TAG
             1577  CTAG                
MfeI          357  CAATTG               C^AATTG
              492  CAATTG              
MunI          357  CAATTG               C^AATTG
              492  CAATTG              
NcoI           47  CCATGG               C^CATGG
              294  CCATGG              
PflFI         403  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1104  GACTTTGTC           
TfiI         1373  GAATC                G^A[AT]TC
             1735  GATTC               
Tth111I       403  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1104  GACTTTGTC           
XmnI          145  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2059  GAAGTGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       Bsp1286I    BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
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