Restriction map for pF1KA0030
 (Linear) (Six-base) MAPSORT of: KIAA0030.seq  Check: 2035  from: 1  to: 2712



KIAA0030.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     869    2517    2712 
   Size:        869    1648     195

  Fragments arranged by size: 

                1648     869     195
 
AatII G_ACGT'C

Cuts at:      0    2372    2712 
   Size:       2372     340
 
AccI GT'mk_AC

Cuts at:      0     620    2712 
   Size:        620    2092
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2179    2527    2640    2712 
   Size:       2179     348     113      72

  Fragments arranged by size: 

                2179     348     113      72
 
AfeI AGC'GCT

Cuts at:      0    2100    2712 
   Size:       2100     612
 
AflIII A'CryG_T

Cuts at:      0     662    2123    2712 
   Size:        662    1461     589

  Fragments arranged by size: 

                1461     662     589
 
AhdI GACnn_n'nnGTC

Cuts at:      0     320    2712 
   Size:        320    2392
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2359    2712 
   Size:       2359     353
 
ApaI G_GGCC'C

Cuts at:      0     185     571    1725    2559    2712 
   Size:        185     386    1154     834     153

  Fragments arranged by size: 

                1154     834     386     185     153
 
ApoI r'AATT_y

Cuts at:      0    1762    2712 
   Size:       1762     950
 
AvrII C'CTAG_G

Cuts at:      0     168    2712 
   Size:        168    2544
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1107    1140    2712 
   Size:       1107      33    1572

  Fragments arranged by size: 

                1572    1107      33
 
BanI G'GyrC_C

Cuts at:      0     750    1685    2712 
   Size:        750     935    1027

  Fragments arranged by size: 

                1027     935     750
 
BanII G_rGCy'C

Cuts at:      0     167     185     215     404     571    1511    1725    1955 
   Size:        167      18      30     189     167     940     214     230

Cuts at:   1955    2111    2141    2559    2654    2712 
   Size:        156      30     418      95      58

  Fragments arranged by size: 

                 940     418     230     214     189     167     167     156
                  95      58      30      30      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     633    1472    2369    2712 
   Size:        633     839     897     343

  Fragments arranged by size: 

                 897     839     633     343
 
BbvCI CC'TCA_GC

Cuts at:      0     889    2712 
   Size:        889    1823
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     600     634    2712 
   Size:        600      34    2078

  Fragments arranged by size: 

                2078     600      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1135    2712 
   Size:       1135    1577
 
BclI T'GATC_A

Cuts at:      0    1388    2712 
   Size:       1388    1324
 
BglI GCCn_nnn'nGGC

Cuts at:      0     271    2712 
   Size:        271    2441
 
BglII A'GATC_T

Cuts at:      0     770    1200    1427    2712 
   Size:        770     430     227    1285

  Fragments arranged by size: 

                1285     770     430     227
 
BlpI GC'TnA_GC

Cuts at:      0     964    2105    2712 
   Size:        964    1141     607

  Fragments arranged by size: 

                1141     964     607
 
Bme1580I G_kGCm'C

Cuts at:      0     185     571    1725    2559    2712 
   Size:        185     386    1154     834     153

  Fragments arranged by size: 

                1154     834     386     185     153
 
BmgBI CAC'GTC

Cuts at:      0     618    2049    2712 
   Size:        618    1431     663

  Fragments arranged by size: 

                1431     663     618
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1999    2712 
   Size:       1999     713
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     229     900    1768    2712 
   Size:        229     671     868     944

  Fragments arranged by size: 

                 944     868     671     229
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     102     208     541     598     811    2416    2712 
   Size:         60      42     106     333      57     213    1605     296

  Fragments arranged by size: 

                1605     333     296     213     106      60      57      42
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     889    2712 
   Size:        889    1823
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1582    2712 
   Size:       1582    1130
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1093    1562    2712 
   Size:       1093     469    1150

  Fragments arranged by size: 

                1150    1093     469
 
BsaAI yAC'GTr

Cuts at:      0     726    1329    1540    2126    2712 
   Size:        726     603     211     586     586

  Fragments arranged by size: 

                 726     603     586     586     211
 
BsaBI GATnn'nnATC

Cuts at:      0    2617    2712 
   Size:       2617      95
 
BsaHI Gr'CG_yC

Cuts at:      0     263     950    1184    1651    2369    2712 
   Size:        263     687     234     467     718     343

  Fragments arranged by size: 

                 718     687     467     343     263     234
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     223     322     436     445     499     508     889    1111 
   Size:        223      99     114       9      54       9     381     222

Cuts at:   1111    1183    1757    2089    2092    2712 
   Size:         72     574     332       3     620

  Fragments arranged by size: 

                 620     574     381     332     223     222     114      99
                  72      54       9       9       3
 
BseYI C'CCAG_C

Cuts at:      0      44     959    1717    2062    2558    2712 
   Size:         44     915     758     345     496     154

  Fragments arranged by size: 

                 915     758     496     345     154      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     927    1854    2712 
   Size:        927     927     858

  Fragments arranged by size: 

                 927     927     858
 
BsiEI CG_ry'CG

Cuts at:      0     825    1073    2712 
   Size:        825     248    1639

  Fragments arranged by size: 

                1639     825     248
 
BsiHKAI G_wGCw'C

Cuts at:      0     215     725    1559    2654    2712 
   Size:        215     510     834    1095      58

  Fragments arranged by size: 

                1095     834     510     215      58
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1220    2712 
   Size:       1220    1492
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     185     215     404     571     725    1511    1559 
   Size:        167      18      30     189     167     154     786      48

Cuts at:   1559    1725    1955    2111    2141    2559    2654    2712 
   Size:        166     230     156      30     418      95      58

  Fragments arranged by size: 

                 786     418     230     189     167     167     166     156
                 154      95      58      48      30      30      18
 
BspHI T'CATG_A

Cuts at:      0    1461    2712 
   Size:       1461    1251
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     869    2517    2712 
   Size:        869    1648     195

  Fragments arranged by size: 

                1648     869     195
 
BsrBI CCG'CTC

Cuts at:      0     342     465    1175    2712 
   Size:        342     123     710    1537

  Fragments arranged by size: 

                1537     710     342     123
 
BsrDI GCAATG_nn'

Cuts at:      0      66    1439    1877    2712 
   Size:         66    1373     438     835

  Fragments arranged by size: 

                1373     835     438      66
 
BsrFI r'CCGG_y

Cuts at:      0     753    1072    1163    2712 
   Size:        753     319      91    1549

  Fragments arranged by size: 

                1549     753     319      91
 
BsrGI T'GTAC_A

Cuts at:      0    2237    2712 
   Size:       2237     475
 
BssHII G'CGCG_C

Cuts at:      0    2332    2712 
   Size:       2332     380
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     992    2712 
   Size:        992    1720
 
BstEII G'GTnAC_C

Cuts at:      0     933    2712 
   Size:        933    1779
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     930    1329    2712 
   Size:        930     399    1383

  Fragments arranged by size: 

                1383     930     399
 
BstYI r'GATC_y

Cuts at:      0     525     581     770    1200    1427    2712 
   Size:        525      56     189     430     227    1285

  Fragments arranged by size: 

                1285     525     430     227     189      56
 
Bsu36I CC'TnA_GG

Cuts at:      0     746    2248    2712 
   Size:        746    1502     464

  Fragments arranged by size: 

                1502     746     464
 
BtgI C'CryG_G

Cuts at:      0      26     396     470     627    1808    1997    2712 
   Size:         26     370      74     157    1181     189     715

  Fragments arranged by size: 

                1181     715     370     189     157      74      26
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     431    2712 
   Size:        431    2281
 
BtsI GCAGTG_nn'

Cuts at:      0    1280    2712 
   Size:       1280    1432
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1521    1556    2712 
   Size:       1521      35    1156

  Fragments arranged by size: 

                1521    1156      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     543    2712 
   Size:        543    2169
 
EaeI y'GGCC_r

Cuts at:      0      84     126     317     372     630     803    1070    1161 
   Size:         84      42     191      55     258     173     267      91

Cuts at:   1161    1331    1641    2087    2228    2378    2712 
   Size:        170     310     446     141     150     334

  Fragments arranged by size: 

                 446     334     310     267     258     191     173     170
                 150     141      91      84      55      42
 
EagI C'GGCC_G

Cuts at:      0    1070    2712 
   Size:       1070    1642
 
EarI CTCTTCn'nnn_

Cuts at:      0     191    2503    2659    2712 
   Size:        191    2312     156      53

  Fragments arranged by size: 

                2312     191     156      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     772    2335    2712 
   Size:         19     753    1563     377

  Fragments arranged by size: 

                1563     753     377      19
 
EcoICRI GAG'CTC

Cuts at:      0     213    2652    2712 
   Size:        213    2439      60

  Fragments arranged by size: 

                2439     213      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     102     208     541     598     811    2179    2416 
   Size:         60      42     106     333      57     213    1368     237

Cuts at:   2416    2527    2640    2712 
   Size:        111     113      72

  Fragments arranged by size: 

                1368     333     237     213     113     111     106      72
                  60      57      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     745    1402    2146    2712 
   Size:        745     657     744     566

  Fragments arranged by size: 

                 745     744     657     566
 
EcoO109I rG'GnC_Cy

Cuts at:      0     181     182     378     568    1009    1721    1722    2154 
   Size:        181       1     196     190     441     712       1     432

Cuts at:   2154    2555    2577    2712 
   Size:        401      22     135

  Fragments arranged by size: 

                 712     441     432     401     196     190     181     135
                  22       1       1
 
EcoRV GAT'ATC

Cuts at:      0    1548    2712 
   Size:       1548    1164
 
FseI GG_CCGG'CC

Cuts at:      0    1076    2712 
   Size:       1076    1636
 
FspI TGC'GCA

Cuts at:      0     610    2434    2443    2712 
   Size:        610    1824       9     269

  Fragments arranged by size: 

                1824     610     269       9
 
HaeII r_GCGC'y

Cuts at:      0     438     453    1908    2102    2712 
   Size:        438      15    1455     194     610

  Fragments arranged by size: 

                1455     610     438     194      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171     306     338    1051    1083    1092    1124 
   Size:        139      32     135      32     713      32       9      32

Cuts at:   1124    1412    1444    1600    1632    2712 
   Size:        288      32     156      32    1080

  Fragments arranged by size: 

                1080     713     288     156     139     135      32      32
                  32      32      32      32       9
 
HincII GTy'rAC

Cuts at:      0     621    1089    2373    2712 
   Size:        621     468    1284     339

  Fragments arranged by size: 

                1284     621     468     339
 
MscI TGG'CCA

Cuts at:      0     805    1333    1643    2089    2230    2380    2712 
   Size:        805     528     310     446     141     150     332

  Fragments arranged by size: 

                 805     528     446     332     310     150     141
 
MslI CAynn'nnrTG

Cuts at:      0     842    2287    2386    2712 
   Size:        842    1445      99     326

  Fragments arranged by size: 

                1445     842     326      99
 
MspA1I CmG'CkG

Cuts at:      0     897     912     941    1214    1683    2712 
   Size:        897      15      29     273     469    1029

  Fragments arranged by size: 

                1029     897     469     273      29      15
 
NaeI GCC'GGC

Cuts at:      0    1074    1165    2712 
   Size:       1074      91    1547

  Fragments arranged by size: 

                1547    1074      91
 
NcoI C'CATG_G

Cuts at:      0      26    1808    2712 
   Size:         26    1782     904

  Fragments arranged by size: 

                1782     904      26
 
NgoMIV G'CCGG_C

Cuts at:      0    1072    1163    2712 
   Size:       1072      91    1549

  Fragments arranged by size: 

                1549    1072      91
 
NspI r_CATG'y

Cuts at:      0     393     666    2442    2712 
   Size:        393     273    1776     270

  Fragments arranged by size: 

                1776     393     273     270
 
PciI A'CATG_T

Cuts at:      0     662    2712 
   Size:        662    2050
 
PfoI T'CCnGG_A

Cuts at:      0    1133    2009    2712 
   Size:       1133     876     703

  Fragments arranged by size: 

                1133     876     703
 
PmlI CAC'GTG

Cuts at:      0     726    1329    2712 
   Size:        726     603    1383

  Fragments arranged by size: 

                1383     726     603
 
PpuMI rG'GwC_Cy

Cuts at:      0    1009    2154    2577    2712 
   Size:       1009    1145     423     135

  Fragments arranged by size: 

                1145    1009     423     135
 
PspOMI G'GGCC_C

Cuts at:      0     181     567    1721    2555    2712 
   Size:        181     386    1154     834     157

  Fragments arranged by size: 

                1154     834     386     181     157
 
PstI C_TGCA'G

Cuts at:      0     770    1862    2705    2712 
   Size:        770    1092     843       7

  Fragments arranged by size: 

                1092     843     770       7
 
PvuII CAG'CTG

Cuts at:      0     897     912     941    1214    2712 
   Size:        897      15      29     273    1498

  Fragments arranged by size: 

                1498     897     273      29      15
 
SacI G_AGCT'C

Cuts at:      0     215    2654    2712 
   Size:        215    2439      58

  Fragments arranged by size: 

                2439     215      58
 
SalI G'TCGA_C

Cuts at:      0     619    2712 
   Size:        619    2093
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2503    2659    2712 
   Size:       2503     156      53

  Fragments arranged by size: 

                2503     156      53
 
SbfI CC_TGCA'GG

Cuts at:      0    1862    2712 
   Size:       1862     850
 
SfcI C'TryA_G

Cuts at:      0     766    1355    1858    2701    2712 
   Size:        766     589     503     843      11

  Fragments arranged by size: 

                 843     766     589     503      11
 
SgrAI Cr'CCGG_yG

Cuts at:      0     753    2712 
   Size:        753    1959
 
SmlI C'TyrA_G

Cuts at:      0    1597    2712 
   Size:       1597    1115
 
SphI G_CATG'C

Cuts at:      0     393    2442    2712 
   Size:        393    2049     270

  Fragments arranged by size: 

                2049     393     270
 
StuI AGG'CCT

Cuts at:      0    1481    2712 
   Size:       1481    1231
 
StyI C'CwwG_G

Cuts at:      0      26     168    1178    1403    1709    1808    2180    2712 
   Size:         26     142    1010     225     306      99     372     532

  Fragments arranged by size: 

                1010     532     372     306     225     142      99      26
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     543    1384    1754    2712 
   Size:        543     841     370     958

  Fragments arranged by size: 

                 958     841     543     370
 
TatI w'GTAC_w

Cuts at:      0     977    2165    2237    2712 
   Size:        977    1188      72     475

  Fragments arranged by size: 

                1188     977     475      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2287    2319    2712 
   Size:       2287      32     393

  Fragments arranged by size: 

                2287     393      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2005    2368    2712 
   Size:       2005     363     344

  Fragments arranged by size: 

                2005     363     344
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255    2313    2712 
   Size:        255    2058     399

  Fragments arranged by size: 

                2058     399     255
 
XmnI GAAnn'nnTTC

Cuts at:      0    2668    2712 
   Size:       2668      44
 
ZraI GAC'GTC

Cuts at:      0    2370    2712 
   Size:       2370     342
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AlfI
     ApaI     ApoI    AvrII     BaeI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    HaeII    Hin4I   HincII     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PciI     PfoI
     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI     SalI     SapI
     SbfI     SfcI    SgrAI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AleI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI     AvaI    BamHI     BbeI    BfrBI     BmtI
    BsaWI    BsaXI    BsiWI     BsmI    BspEI    BssSI    BstBI  BstZ17I
     ClaI     DraI     DrdI    EcoRI     FalI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NarI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII    SanDI
     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI     XbaI     XhoI     XmaI
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