Restriction map for pF1KA0012
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     FauI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AflII        2313  CTTAAG               C^TTAAG
AflIII       1712  ACATGT               A^C[GA][CT]GT
AlwNI         995  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          541  CCTGAGG              CC^T[ACGT]AGG
AseI         2326  ATTAAT               AT^TAAT
AsnI         2326  ATTAAT               AT^TAAT
AspHI         438  GAGCAC               G[AT]GC[AT]^C
BalI         2249  TGGCCA               TGG^CCA
BamHI         788  GGATCC               G^GATCC
BfrI         2313  CTTAAG               C^TTAAG
BsaI          103  GGTCTC               GGTCTC(1/5)
BsgI          574  CTGCAC               GTGCAG(16/14)
BsiHKAI       438  GAGCAC               G[AT]GC[AT]^C
BsmI         1376  GCATTC               GAATGC(1/-1)
BspLU11I     1712  ACATGT               A^CATGT
BspMI        2053  ACCTGC               ACCTGC(4/8)
BstAPI        373  GCATTTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         969  TTCGAA               TT^CGAA
BstXI        1763  CCACCATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        541  CCTGAGG              CC^T[ACGT]AGG
CfoI         1860  GCGC                 GCG^C
DraI         1281  TTTAAA               TTT^AAA
DraII         545  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        577  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         2249  TGGCCA               [CT]^GGCC[GA]
EarI         2064  GAAGAG               CTCTTC(1/4)
Eco81I        541  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1695  CCTACTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      545  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         253  GATATC               GAT^ATC
EcoT14I      2276  CCAAGG               C^C[AT][AT]GG
HhaI         1860  GCGC                 GCG^C
HinP1I       1860  GCGC                 G^CGC
HindIII      1403  AAGCTT               A^AGCTT
Ksp632I      2064  GAAGAG               CTCTTC(1/4)
MluNI        2249  TGGCCA               TGG^CCA
MscI         2249  TGGCCA               TGG^CCA
NspV          969  TTCGAA               TT^CGAA
PflMI         276  CCAGGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          570  GAGTC                GAGTC(4/5)
PshBI        2326  ATTAAT               AT^TAAT
PstI         1800  CTGCAG               CTGCA^G
ScaI         2207  AGTACT               AGT^ACT
SfuI          969  TTCGAA               TT^CGAA
SspI         2329  AATATT               AAT^ATT
StuI          899  AGGCCT               AGG^CCT
StyI         2276  CCAAGG               C^C[AT][AT]GG
TaqI          970  TCGA                 T^CGA
TthHB8I       970  TCGA                 T^CGA
Van91I        276  CCAGGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1766  CCATGCTGGTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AciI          943  CCGC                 CCGC(-3/-1)
             2203  CCGC                
Asp700I       774  GAATTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2194  GAACCCATTC          
BanI         1106  GGCACC               G^G[CT][GA]CC
             2117  GGTGCC              
BbsI          535  GAAGAC               GAAGAC(2/6)
             1290  GTCTTC              
BclI         1484  TGATCA               T^GATCA
             1491  TGATCA              
BcnI         1674  CCCGG                CC^[GC]GG
             1856  CCCGG               
BpmI          793  CTCCAG               CTGGAG(16/14)
             1903  CTCCAG              
BpuAI         535  GAAGAC               GAAGAC(2/6)
             1290  GTCTTC              
BsmFI        1565  GGGAC                GGGAC(10/14)
              300  GTCCC               
BspHI        2004  TCATGA               T^CATGA
             2157  TCATGA              
Cac8I        1646  GCTGGC               GC[ACGT]^[ACGT]GC
             2346  GCAAGC              
EcoT22I       371  ATGCAT               ATGCA^T
             1913  ATGCAT              
FbaI         1484  TGATCA               T^GATCA
             1491  TGATCA              
HapII        1675  CCGG                 C^CGG
             1857  CCGG                
HpaII        1675  CCGG                 C^CGG
             1857  CCGG                
MfeI          473  CAATTG               C^AATTG
             1204  CAATTG              
MslI         1574  CATTCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1767  CATGCTGGTG          
MspA1I        796  CAGCTG               C[AC]G^C[GT]G
              856  CAGCTG              
MspI         1675  CCGG                 C^CGG
             1857  CCGG                
MunI          473  CAATTG               C^AATTG
             1204  CAATTG              
NciI         1674  CCCGG                CC^[GC]GG
             1856  CCCGG               
NsiI          371  ATGCAT               ATGCA^T
             1913  ATGCAT              
NspI         1019  ACATGC               [GA]CATG^[CT]
             1712  ACATGT              
PvuII         796  CAGCTG               CAG^CTG
              856  CAGCTG              
RcaI         2004  TCATGA               T^CATGA
             2157  TCATGA              
SfaNI         379  GATGC                GCATC(5/9)
                8  GCATC               
SmlI          346  CTCAAG               C^T[CT][GA]AG
             2313  CTTAAG              
XmnI          774  GAATTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2194  GAACCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BbvI        BfaI        BmyI        BsaJI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cfr13I      
DdeI        DpnII       EcoRI       EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
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