Restriction map for pF1KB7869
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BalI        
BamHI       BbvCI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BsaBI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HpaI        KpnI        
MaeI        MamI        MfeI        MluI        MluNI       
MscI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII        739  TCCGGA               T^CCGGA
AlwI          306  GATCC                GGATC(4/5)
AlwNI         304  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AviII         549  TGCGCA               TGC^GCA
BanI           84  GGCGCC               G^G[CT][GA]CC
BbeI           84  GGCGCC               GGCGC^C
BbrPI         979  CACGTG               CAC^GTG
BcgI          373  CGAGAGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          648  GCCACAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          243  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      243  GCTGAGC              GC^T[ACGT]AGC
BsaAI         979  CACGTG               [CT]AC^GT[GA]
BseAI         739  TCCGGA               T^CCGGA
BsgI          193  CTGCAC               GTGCAG(16/14)
BspEI         739  TCCGGA               T^CCGGA
BsrBI         390  GAGCGG               CCGCTC(-3/-3)
BsrFI         248  GCCGGC               [GA]^CCGG[CT]
BstXI        1164  CCAAGAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         305  AGATCC               [GA]^GATC[CT]
CelII         243  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        248  GCCGGC               [GA]^CCGG[CT]
DpnII         306  GATC                 ^GATC
DraII          48  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        964  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          407  TGGCCG               [CT]^GGCC[GA]
EcoNI         345  CCTGCCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       48  GGGACCC              [GA]G^G[ACGT]CC[CT]
FspI          549  TGCGCA               TGC^GCA
HindIII      1179  AAGCTT               A^AGCTT
KasI           84  GGCGCC               G^GCGCC
KspI          161  CCGCGG               CCGC^GG
MboI          306  GATC                 ^GATC
MflI          305  AGATCC               [GA]^GATC[CT]
MroI          739  TCCGGA               T^CCGGA
NaeI          248  GCCGGC               GCC^GGC
NarI           84  GGCGCC               GG^CGCC
NdeII         306  GATC                 ^GATC
NgoMIV        248  GCCGGC               G^CCGGC
PmaCI         979  CACGTG               CAC^GTG
PmlI          979  CACGTG               CAC^GTG
PpuMI          48  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         205  CAGCTG               CAG^CTG
SacII         161  CCGCGG               CCGC^GG
SapI         1425  GAAGAGC              GCTCTTC(1/4)
Sau3AI        306  GATC                 ^GATC
SfaNI         797  GCATC                GCATC(5/9)
SfoI           84  GGCGCC               GGC^GCC
XcmI          216  CCAGGAGGTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         305  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI           37  GACGCC               G[GA]^CG[CT]C
               84  GGCGCC              
AspHI         376  GAGCAC               G[AT]GC[AT]^C
              567  GAGCAC              
AvaI          283  CCCGAG               C^[CT]CG[GA]G
              349  CCCGAG              
BanII         148  GAGCCC               G[GA]GC[CT]^C
              419  GGGCTC              
BbsI          127  GAAGAC               GAAGAC(2/6)
              835  GAAGAC              
BcnI          136  CCCGG                CC^[GC]GG
              563  CCGGG               
BpmI          310  CTGGAG               CTGGAG(16/14)
              325  CTGGAG              
BpuAI         127  GAAGAC               GAAGAC(2/6)
              835  GAAGAC              
BsaHI          37  GACGCC               G[GA]^CG[CT]C
               84  GGCGCC              
BsaWI         231  ACCGGA               [AT]^CCGG[AT]
              739  TCCGGA              
BsiHKAI       376  GAGCAC               G[AT]GC[AT]^C
              567  GAGCAC              
BsmI         1336  GAATGC               GAATGC(1/-1)
             1417  GAATGC              
BsoBI         283  CCCGAG               C^[CT]CG[GA]G
              349  CCCGAG              
BsrI         1474  ACTGG                ACTGG(1/-1)
             1558  ACTGG               
DraI         1324  TTTAAA               TTT^AAA
             1453  TTTAAA              
DsaI          161  CCGCGG               C^C[GA][CT]GG
              410  CCGTGG              
EcoT14I        74  CCAAGG               C^C[AT][AT]GG
              296  CCAAGG              
HaeIII        408  GGCC                 GG^CC
              917  GGCC                
HgaI           37  GACGC                GACGC(5/10)
               79  GACGC               
Hin1I          37  GACGCC               G[GA]^CG[CT]C
               84  GGCGCC              
MaeII         973  ACGT                 A^CGT
              980  ACGT                
MslI          477  CACGGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1138  CACACAGGTG          
MspA1I        161  CCGCGG               C[AC]G^C[GT]G
              205  CAGCTG              
NciI          136  CCCGG                CC^[GC]GG
              563  CCGGG               
PflMI         216  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              453  CCAGGGGATGG         
PstI          424  CTGCAG               CTGCA^G
              537  CTGCAG              
SfcI          424  CTGCAG               C^T[GA][CT]AG
              537  CTGCAG              
StyI           74  CCAAGG               C^C[AT][AT]GG
              296  CCAAGG              
Van91I        216  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              453  CCAGGGGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
Aor51HI     ApoI        Asp700I     AvaII       BbvI        
BmyI        BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        EarI        Eco47III    EcoRI       
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboII       
MnlI        MseI        MspI        MvaI        MvnI        
MwoI        NlaIII      NlaIV       PleI        RsaI        
Sau96I      ScrFI       TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XmnI        
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