Restriction map for pF1KB9574
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaI        BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraIII      Eam1105I    
Eco47III    Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        210  TCCGGA               T^CCGGA
AcyI           33  GGCGTC               G[GA]^CG[CT]C
AfaI          989  GTAC                 GT^AC
AflIII       1010  ACACGT               A^C[GA][CT]GT
Alw44I        969  GTGCAC               G^TGCAC
AlwI          615  GATCC                GGATC(4/5)
AlwNI         220  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          940  GGGCCC               GGGCC^C
ApaLI         969  GTGCAC               G^TGCAC
AspHI         969  GTGCAC               G[AT]GC[AT]^C
BalI          947  TGGCCA               TGG^CCA
BcgI          601  CGACCTGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1113  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1113  GCTCAGC              GC^T[ACGT]AGC
BsaHI          33  GGCGTC               G[GA]^CG[CT]C
BseAI         210  TCCGGA               T^CCGGA
BsiEI          65  CGGCCG               CG[GA][CT]^CG
BsiHKAI       969  GTGCAC               G[AT]GC[AT]^C
BspEI         210  TCCGGA               T^CCGGA
BsrBI         858  CCGCTC               CCGCTC(-3/-3)
BsrDI        1091  GCAATG               GCAATG(2/0)
BstEII        981  GGTCACC              G^GT[ACGT]ACC
BstPI         981  GGTCACC              G^GT[ACGT]ACC
BstYI         614  AGATCC               [GA]^GATC[CT]
CelII        1113  GCTCAGC              GC^T[ACGT]AGC
DrdI          154  GACCTGGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           65  CGGCCG               C^GGCCG
EclXI          65  CGGCCG               C^GGCCG
Eco52I         65  CGGCCG               C^GGCCG
EcoO65I       981  GGTCACC              G^GT[ACGT]ACC
EcoT14I       944  CCATGG               C^C[AT][AT]GG
Hin1I          33  GGCGTC               G[GA]^CG[CT]C
HincII        130  GTCAAC               GT[CT]^[GA]AC
HindII        130  GTCAAC               GT[CT]^[GA]AC
MaeII        1012  ACGT                 A^CGT
MflI          614  AGATCC               [GA]^GATC[CT]
MluNI         947  TGGCCA               TGG^CCA
MroI          210  TCCGGA               T^CCGGA
MscI          947  TGGCCA               TGG^CCA
MslI          972  CACTCCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          944  CCATGG               C^CATGG
PpuMI         886  GGGTCCT              [GA]G^G[AT]CC[CT]
RsaI          989  GTAC                 GT^AC
StuI          710  AGGCCT               AGG^CCT
StyI          944  CCATGG               C^C[AT][AT]GG
TaqI          202  TCGA                 T^CGA
TfiI          422  GAATC                G^A[AT]TC
TthHB8I       202  TCGA                 T^CGA
XcmI          746  CCAAGCCCTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         614  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII          57  CGCG                 CG^CG
              135  CGCG                
AvaI            9  CTCGGG               C^[CT]CG[GA]G
             1036  CCCGAG              
BfaI          514  CTAG                 C^TAG
              577  CTAG                
BsmBI         162  CGTCTC               CGTCTC(1/5)
              243  CGTCTC              
BsmFI          60  GTCCC                GGGAC(10/14)
              292  GTCCC               
BsoBI           9  CTCGGG               C^[CT]CG[GA]G
             1036  CCCGAG              
BstUI          57  CGCG                 CG^CG
              135  CGCG                
BstXI         950  CCACAGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1124  CCACGCTGCTGG        
DpnII         615  GATC                 ^GATC
              664  GATC                
DsaI          185  CCACGG               C^C[GA][CT]GG
              944  CCATGG              
EarI          409  GAAGAG               CTCTTC(1/4)
              640  GAAGAG              
EcoNI         555  CCTGCTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              678  CCTGCCAGAGG         
HgaI           34  GCGTC                GACGC(5/10)
               58  GCGTC               
Ksp632I       409  GAAGAG               CTCTTC(1/4)
              640  GAAGAG              
MaeI          514  CTAG                 C^TAG
              577  CTAG                
MaeIII        190  GTCAC                ^GT[ACGT]AC
              982  GTCAC               
MboI          615  GATC                 ^GATC
              664  GATC                
MvnI           57  CGCG                 CG^CG
              135  CGCG                
NdeII         615  GATC                 ^GATC
              664  GATC                
Sau3AI        615  GATC                 ^GATC
              664  GATC                
SfaNI         773  GCATC                GCATC(5/9)
              826  GCATC               
Tsp45I        190  GTCAC                ^GT[GC]AC
              982  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbsI        
BbvI        BcnI        BmyI        BpmI        BpuAI       
BsaJI       BsaWI       BseRI       BsgI        BsiYI       
BslI        BsmAI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       PleI        PstI        PvuII       Sau96I      
ScrFI       SfcI        TseI        TspRI       
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