Restriction map for pF1KB9538
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflIII      AgeI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BcnI        
BfaI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaJI       BsiEI       BsiWI       BsmBI       BsoBI       
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MflI        MluI        MluNI       
MscI        MspA1I      MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        561  TCCGGA               T^CCGGA
AciI          905  GCGG                 CCGC(-3/-1)
AflII          52  CTTAAG               C^TTAAG
AhdI         1016  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1016  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         614  GAGCAC               G[AT]GC[AT]^C
BbvCI         327  CCTCAGC              CCTCAGC(-5/-2)
BbvI          340  GCAGC                GCAGC(8/12)
BfrI           52  CTTAAG               C^TTAAG
BmyI          614  GAGCAC               G[AGT]GC[ACT]^C
BpmI          455  CTGGAG               CTGGAG(16/14)
BsaI            8  GAGACC               GGTCTC(1/5)
BsaWI         561  TCCGGA               [AT]^CCGG[AT]
BseAI         561  TCCGGA               T^CCGGA
BseRI         427  CTCCTC               GAGGAG(10/8)
BsiHKAI       614  GAGCAC               G[AT]GC[AT]^C
BsmAI           8  GAGAC                GTCTC(1/5)
BsmFI        1011  GTCCC                GGGAC(10/14)
BsmI          472  GAATGC               GAATGC(1/-1)
Bsp1286I      614  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I      603  TGTACA               T^GTACA
BspEI         561  TCCGGA               T^CCGGA
BsrDI         476  GCAATG               GCAATG(2/0)
BsrGI         603  TGTACA               T^GTACA
Cac8I          30  GCAGGC               GC[ACGT]^[ACGT]GC
DpnII          85  GATC                 ^GATC
DraI          349  TTTAAA               TTT^AAA
Eam1105I     1016  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         998  CCTTTAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII         562  CCGG                 C^CGG
HincII        927  GTCAAC               GT[CT]^[GA]AC
HindII        927  GTCAAC               GT[CT]^[GA]AC
HindIII       575  AAGCTT               A^AGCTT
HpaII         562  CCGG                 C^CGG
MboI           85  GATC                 ^GATC
MfeI           68  CAATTG               C^AATTG
MroI          561  TCCGGA               T^CCGGA
MspI          562  CCGG                 C^CGG
MunI           68  CAATTG               C^AATTG
NdeII          85  GATC                 ^GATC
NlaIII        861  CATG                 CATG^
PleI         1016  GACTC                GAGTC(4/5)
Sau3AI         85  GATC                 ^GATC
SmlI           52  CTTAAG               C^T[CT][GA]AG
SspBI         603  TGTACA               T^GTACA
SspI          296  AATATT               AAT^ATT
StuI          995  AGGCCT               AGG^CCT
TaqI          554  TCGA                 T^CGA
TseI          340  GCAGC                G^C[AT]GC
TthHB8I       554  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

Asp700I       489  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              546  GAAACCTTTC          
BsgI          642  GTGCAG               GTGCAG(16/14)
              810  GTGCAG              
EcoRI         530  GAATTC               G^AATTC
              698  GAATTC              
Fnu4HI        340  GCAGC                GC^[ACGT]GC
              905  GCGGC               
HaeIII        507  GGCC                 GG^CC
              996  GGCC                
HphI          581  TCACC                GGTGA(8/7)
              683  TCACC               
ItaI          340  GCAGC                GC^[ACGT]GC
              905  GCGGC               
MboII         627  GAAGA                GAAGA(8/7)
              780  GAAGA               
MwoI           30  GCAGGCGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              803  GCTATGAGTGC         
TfiI          783  GATTC                G^A[AT]TC
              950  GAATC               
Tsp45I        401  GTGAC                ^GT[GC]AC
              423  GTCAC               
XmnI          489  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              546  GAAACCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsiYI       
BslI        BsrI        DdeI        HinfI       MaeIII      
MnlI        MseI        MslI        NdeI        RsaI        
Tru9I       Tsp509I     TspRI       
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