Restriction map for pF1KB9519
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwNI       Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstXI       BstYI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MflI        MluI        MroI        MseI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Van91I      
XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          607  GTCTAC               GT^[AC][GT]AC
AfaI           79  GTAC                 GT^AC
Alw44I        275  GTGCAC               G^TGCAC
AlwI          734  GGATC                GGATC(4/5)
ApaI          754  GGGCCC               GGGCC^C
ApaLI         275  GTGCAC               G^TGCAC
AvaI          703  CCCGGG               C^[CT]CG[GA]G
BanII         754  GGGCCC               G[GA]GC[CT]^C
BcgI          869  CGATCACACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         779  GTATCC               GTATCC(6/5)
BglI          298  GCCTTTATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI          13  ACCGGA               [AT]^CCGG[AT]
BsoBI         703  CCCGGG               C^[CT]CG[GA]G
BsrBI          33  GAGCGG               CCGCTC(-3/-3)
BstAPI        660  GCAGATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        896  GGTCACC              G^GT[ACGT]ACC
BstF5I        980  GGATG                GGATG(2/0)
BstPI         896  GGTCACC              G^GT[ACGT]ACC
DraII        1034  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoNI         156  CCTGCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1034  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       896  GGTCACC              G^GT[ACGT]ACC
FokI          980  GGATG                GGATG(9/13)
HaeII          37  GGCGCT               [GA]GCGC^[CT]
HgaI          353  GCGTC                GACGC(5/10)
HincII       1003  GTCAAC               GT[CT]^[GA]AC
HindII       1003  GTCAAC               GT[CT]^[GA]AC
HinfI        1056  GACTC                G^A[ACGT]TC
MaeII         482  ACGT                 A^CGT
MfeI         1010  CAATTG               C^AATTG
MunI         1010  CAATTG               C^AATTG
PleI         1056  GACTC                GAGTC(4/5)
PpuMI        1034  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI           79  GTAC                 GT^AC
SapI          257  GCTCTTC              GCTCTTC(1/4)
SfiI          297  GGCCTTTATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          703  CCCGGG               CCC^GGG
SphI          795  GCATGC               GCATG^C
TaqI          629  TCGA                 T^CGA
Tsp509I      1011  AATT                 ^AATT
TthHB8I       629  TCGA                 T^CGA
XcmI          548  CCAATGACACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         703  CCCGGG               C^CCGGG
XmaI          703  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         420  CGCG                 CG^CG
              867  CGCG                
AocI           22  CCTGAGG              CC^T[ACGT]AGG
             1050  CCTGAGG             
AspI          444  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              826  GACGAGGTC           
BsgI          157  CTGCAC               GTGCAG(16/14)
              658  GTGCAG              
BstUI         420  CGCG                 CG^CG
              867  CGCG                
Bsu36I         22  CCTGAGG              CC^T[ACGT]AGG
             1050  CCTGAGG             
DpnII         735  GATC                 ^GATC
              870  GATC                
EarI          258  CTCTTC               CTCTTC(1/4)
              625  CTCTTC              
Eco81I         22  CCTGAGG              CC^T[ACGT]AGG
             1050  CCTGAGG             
EcoT14I       458  CCATGG               C^C[AT][AT]GG
              696  CCATGG              
FauI          359  CCCGC                CCCGC(4/6)
              112  GCGGG               
Ksp632I       258  CTCTTC               CTCTTC(1/4)
              625  CTCTTC              
MboI          735  GATC                 ^GATC
              870  GATC                
MboII         259  TCTTC                GAAGA(8/7)
              626  TCTTC               
MslI          429  CATCTGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              567  CATGCTTATG          
MspA1I        282  CAGCTG               C[AC]G^C[GT]G
              847  CAGCTG              
MvnI          420  CGCG                 CG^CG
              867  CGCG                
NaeI          739  GCCGGC               GCC^GGC
              806  GCCGGC              
NcoI          458  CCATGG               C^CATGG
              696  CCATGG              
NdeII         735  GATC                 ^GATC
              870  GATC                
NgoMIV        739  GCCGGC               G^CCGGC
              806  GCCGGC              
PflFI         444  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              826  GACGAGGTC           
PvuII         282  CAGCTG               CAG^CTG
              847  CAGCTG              
Sau3AI        735  GATC                 ^GATC
              870  GATC                
SmlI          142  CTCAAG               C^T[CT][GA]AG
             1039  CTCAAG              
StyI          458  CCATGG               C^C[AT][AT]GG
              696  CCATGG              
Tth111I       444  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              826  GACGAGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AluI        AspHI       AvaII       BalI        
BanI        BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrFI       BsrI        BssKI       BstNI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DsaI        EaeI        EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MaeIII      MluNI       MnlI        MscI        
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       NspI        Sau96I      ScrFI       SfaNI       
TseI        Tsp45I      TspRI       
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