Restriction map for pF1KB9518
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AlwI           15  GATCC                GGATC(4/5)
Asp700I       514  GAACTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          634  CCCGGG               C^[CT]CG[GA]G
BfaI         1070  CTAG                 C^TAG
BglI           21  GCCGCGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         993  AGATCT               A^GATCT
BpmI          806  CTGGAG               CTGGAG(16/14)
BsaI          310  GAGACC               GGTCTC(1/5)
BseRI        1430  GAGGAG               GAGGAG(10/8)
BsmFI          38  GTCCC                GGGAC(10/14)
BsmI         1546  GAATGC               GAATGC(1/-1)
BsoBI         634  CCCGGG               C^[CT]CG[GA]G
BspHI        1577  TCATGA               T^CATGA
BsrBI         331  GAGCGG               CCGCTC(-3/-3)
BsrFI          54  GCCGGT               [GA]^CCGG[CT]
BstAPI        451  GCAGCAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I         54  GCCGGT               [GA]^CCGG[CT]
EaeI           48  CGGCCA               [CT]^GGCC[GA]
EcoRI        1392  GAATTC               G^AATTC
EcoT22I      1003  ATGCAT               ATGCA^T
FauI           18  CCCGC                CCCGC(4/6)
HaeII         685  GGCGCT               [GA]GCGC^[CT]
HgaI         1226  GCGTC                GACGC(5/10)
KspI           22  CCGCGG               CCGC^GG
MaeI         1070  CTAG                 C^TAG
NdeI          408  CATATG               CA^TATG
NsiI         1003  ATGCAT               ATGCA^T
NspI          143  GCATGC               [GA]CATG^[CT]
PvuII        1566  CAGCTG               CAG^CTG
RcaI         1577  TCATGA               T^CATGA
SacI         1747  GAGCTC               GAGCT^C
SacII          22  CCGCGG               CCGC^GG
SexAI        1347  ACCTGGT              A^CC[AT]GGT
SmaI          634  CCCGGG               CCC^GGG
SphI          143  GCATGC               GCATG^C
SspI          838  AATATT               AAT^ATT
XcmI         1302  CCATGAAATGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         634  CCCGGG               C^CCGGG
XmaI          634  CCCGGG               C^CCGGG
XmnI          514  GAACTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          23  CGCG                 CG^CG
               46  CGCG                
AspHI         809  GAGCAC               G[AT]GC[AT]^C
             1747  GAGCTC              
BanII        1195  GGGCTC               G[GA]GC[CT]^C
             1747  GAGCTC              
BbvI          451  GCAGC                GCAGC(8/12)
             1699  GCAGC               
BsiHKAI       809  GAGCAC               G[AT]GC[AT]^C
             1747  GAGCTC              
BstUI          23  CGCG                 CG^CG
               46  CGCG                
BstYI          14  AGATCC               [GA]^GATC[CT]
              993  AGATCT              
Cac8I          50  GCCAGC               GC[ACGT]^[ACGT]GC
              143  GCATGC              
CfoI           45  GCGC                 GCG^C
              686  GCGC                
DpnII          15  GATC                 ^GATC
              994  GATC                
HaeIII         49  GGCC                 GG^CC
             1459  GGCC                
HhaI           45  GCGC                 GCG^C
              686  GCGC                
HinP1I         45  GCGC                 G^CGC
              686  GCGC                
MboI           15  GATC                 ^GATC
              994  GATC                
MflI           14  AGATCC               [GA]^GATC[CT]
              993  AGATCT              
MslI         1620  CACTGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1626  CATGGCTGTG          
MspA1I         22  CCGCGG               C[AC]G^C[GT]G
             1566  CAGCTG              
MvnI           23  CGCG                 CG^CG
               46  CGCG                
NcoI          824  CCATGG               C^CATGG
             1625  CCATGG              
NdeII          15  GATC                 ^GATC
              994  GATC                
PflMI          51  CCAGCCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1302  CCATGAAATGG         
PstI         1141  CTGCAG               CTGCA^G
             1697  CTGCAG              
Sau3AI         15  GATC                 ^GATC
              994  GATC                
SfaNI        1668  GATGC                GCATC(5/9)
             1643  GCATC               
SfcI         1141  CTGCAG               C^T[GA][CT]AG
             1697  CTGCAG              
SmlI          725  CTCAAG               C^T[CT][GA]AG
             1706  CTCAAG              
TseI          451  GCAGC                G^C[AT]GC
             1699  GCAGC               
Van91I         51  CCAGCCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1302  CCATGAAATGG         
XhoII          14  AGATCC               [GA]^GATC[CT]
              993  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbsI        BcnI        BmyI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DsaI        EcoRII      EcoT14I     Fnu4HI      
FokI        HapII       HinfI       HpaII       HphI        
ItaI        MaeIII      MboII       MnlI        MseI        
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       StyI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       TthHB8I     
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