Restriction map for pF1KB9518
 (Linear) (Six-base) MAPSORT of: KIBB9518.seq  Check: 6875  from: 1  to: 1791



KIBB9518.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     140     250     877    1791 
   Size:        100      40     110     627     914

  Fragments arranged by size: 

                 914     627     110     100      40
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1049    1081    1791 
   Size:       1049      32     710

  Fragments arranged by size: 

                1049     710      32
 
ApoI r'AATT_y

Cuts at:      0     976    1096    1281    1392    1552    1791 
   Size:        976     120     185     111     160     239

  Fragments arranged by size: 

                 976     239     185     160     120     111
 
AvaI C'yCGr_G

Cuts at:      0     634    1791 
   Size:        634    1157
 
BanII G_rGCy'C

Cuts at:      0    1199    1751    1791 
   Size:       1199     552      40

  Fragments arranged by size: 

                1199     552      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      88     599    1195    1566    1791 
   Size:         88     511     596     371     225

  Fragments arranged by size: 

                 596     511     371     225      88
 
BfrBI ATG'CAT

Cuts at:      0    1005    1791 
   Size:       1005     786
 
BglI GCCn_nnn'nGGC

Cuts at:      0      27    1791 
   Size:         27    1764
 
BglII A'GATC_T

Cuts at:      0     993    1791 
   Size:        993     798
 
Bme1580I G_kGCm'C

Cuts at:      0     292    1791 
   Size:        292    1499
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     691    1202    1379    1791 
   Size:        691     511     177     412

  Fragments arranged by size: 

                 691     511     412     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827    1791 
   Size:        827     964
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710    1691    1791 
   Size:        710     981     100

  Fragments arranged by size: 

                 981     710     100
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     304    1791 
   Size:        304    1487
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1657    1687    1791 
   Size:       1657      30     104

  Fragments arranged by size: 

                1657     104      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1445    1791 
   Size:       1445     346
 
BseYI C'CCAG_C

Cuts at:      0     139     785    1568    1791 
   Size:        139     646     783     223

  Fragments arranged by size: 

                 783     646     223     139
 
BsiHKAI G_wGCw'C

Cuts at:      0     813    1751    1791 
   Size:        813     938      40

  Fragments arranged by size: 

                 938     813      40
 
BsmI GAATG_Cn'

Cuts at:      0    1552    1791 
   Size:       1552     239
 
Bsp1286I G_dGCh'C

Cuts at:      0     292     813    1199    1751    1791 
   Size:        292     521     386     552      40

  Fragments arranged by size: 

                 552     521     386     292      40
 
BspHI T'CATG_A

Cuts at:      0    1577    1791 
   Size:       1577     214
 
BsrBI CCG'CTC

Cuts at:      0     333    1791 
   Size:        333    1458
 
BsrFI r'CCGG_y

Cuts at:      0      54    1791 
   Size:         54    1737
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     457    1791 
   Size:        457    1334
 
BstYI r'GATC_y

Cuts at:      0      14     993    1791 
   Size:         14     979     798

  Fragments arranged by size: 

                 979     798      14
 
BtgI C'CryG_G

Cuts at:      0      22     824    1625    1791 
   Size:         22     802     801     166

  Fragments arranged by size: 

                 802     801     166      22
 
BtsI GCAGTG_nn'

Cuts at:      0    1150    1619    1791 
   Size:       1150     469     172

  Fragments arranged by size: 

                1150     469     172
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     545     580    1791 
   Size:        545      35    1211

  Fragments arranged by size: 

                1211     545      35
 
EaeI y'GGCC_r

Cuts at:      0      48    1791 
   Size:         48    1743
 
EcoICRI GAG'CTC

Cuts at:      0    1749    1791 
   Size:       1749      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     140     250     827     877    1791 
   Size:        100      40     110     577      50     914

  Fragments arranged by size: 

                 914     577     110     100      50      40
 
EcoRI G'AATT_C

Cuts at:      0    1392    1791 
   Size:       1392     399
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     665    1459    1791 
   Size:        665     794     332

  Fragments arranged by size: 

                 794     665     332
 
HaeII r_GCGC'y

Cuts at:      0     689    1791 
   Size:        689    1102
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1095    1452    1791 
   Size:       1095     357     339

  Fragments arranged by size: 

                1095     357     339
 
MslI CAynn'nnrTG

Cuts at:      0    1624    1630    1791 
   Size:       1624       6     161

  Fragments arranged by size: 

                1624     161       6
 
MspA1I CmG'CkG

Cuts at:      0      24    1568    1791 
   Size:         24    1544     223

  Fragments arranged by size: 

                1544     223      24
 
NcoI C'CATG_G

Cuts at:      0     824    1625    1791 
   Size:        824     801     166

  Fragments arranged by size: 

                 824     801     166
 
NdeI CA'TA_TG

Cuts at:      0     409    1791 
   Size:        409    1382
 
NsiI A_TGCA'T

Cuts at:      0    1007    1791 
   Size:       1007     784
 
NspI r_CATG'y

Cuts at:      0     147    1791 
   Size:        147    1644
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      57    1308    1791 
   Size:         57    1251     483

  Fragments arranged by size: 

                1251     483      57
 
PsiI TTA'TAA

Cuts at:      0    1165    1791 
   Size:       1165     626
 
PstI C_TGCA'G

Cuts at:      0    1145    1701    1791 
   Size:       1145     556      90

  Fragments arranged by size: 

                1145     556      90
 
PvuII CAG'CTG

Cuts at:      0    1568    1791 
   Size:       1568     223
 
SacI G_AGCT'C

Cuts at:      0    1751    1791 
   Size:       1751      40
 
SacII CC_GC'GG

Cuts at:      0      25    1791 
   Size:         25    1766
 
SexAI A'CCwGG_T

Cuts at:      0    1347    1791 
   Size:       1347     444
 
SfcI C'TryA_G

Cuts at:      0    1141    1697    1791 
   Size:       1141     556      94

  Fragments arranged by size: 

                1141     556      94
 
SmaI CCC'GGG

Cuts at:      0     636    1791 
   Size:        636    1155
 
SmlI C'TyrA_G

Cuts at:      0     725    1706    1791 
   Size:        725     981      85

  Fragments arranged by size: 

                 981     725      85
 
SphI G_CATG'C

Cuts at:      0     147    1791 
   Size:        147    1644
 
SspI AAT'ATT

Cuts at:      0     840    1791 
   Size:        840     951
 
StyI C'CwwG_G

Cuts at:      0     824    1625    1661    1791 
   Size:        824     801      36     130

  Fragments arranged by size: 

                 824     801     130      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     239    1791 
   Size:        239    1552
 
TatI w'GTAC_w

Cuts at:      0     376    1255    1693    1791 
   Size:        376     879     438      98

  Fragments arranged by size: 

                 879     438     376      98
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1309    1791 
   Size:       1309     482
 
XmaI C'CCGG_G

Cuts at:      0     634    1791 
   Size:        634    1157
 
XmnI GAAnn'nnTTC

Cuts at:      0     518    1791 
   Size:        518    1273
 

 Enzymes that do cut:

     AcuI     AloI     ApoI     AvaI    BanII     BbsI    BfrBI     BglI
    BglII Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaXI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrBI    BsrFI   BstAPI
    BstYI     BtgI     BtsI    CspCI     EaeI  EcoICRI  Eco57MI    EcoRI
     FalI    HaeII     MmeI     MslI   MspA1I     NcoI     NdeI     NsiI
     NspI    PflMI     PsiI     PstI    PvuII     SacI    SacII    SexAI
     SfcI     SmaI     SmlI     SphI     SspI     StyI    TaqII     TatI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI
     EagI     EarI     EciI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     ZraI
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