Restriction map for pF1KB9515
 (Linear) (Six-base) MAPSORT of: KIBB9515.seq  Check: 1265  from: 1  to: 1533



KIBB9515.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     672    1533 
   Size:        672     861
 
AccI GT'mk_AC

Cuts at:      0      34     459     941    1533 
   Size:         34     425     482     592

  Fragments arranged by size: 

                 592     482     425      34
 
Acc65I G'GTAC_C

Cuts at:      0     860    1533 
   Size:        860     673
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357    1533 
   Size:        357    1176
 
AfeI AGC'GCT

Cuts at:      0    1338    1533 
   Size:       1338     195
 
AflIII A'CryG_T

Cuts at:      0     516    1533 
   Size:        516    1017
 
AlwNI CAG_nnn'CTG

Cuts at:      0     256     585    1533 
   Size:        256     329     948

  Fragments arranged by size: 

                 948     329     256
 
ApoI r'AATT_y

Cuts at:      0     228     410     799    1032    1533 
   Size:        228     182     389     233     501

  Fragments arranged by size: 

                 501     389     233     228     182
 
AseI AT'TA_AT

Cuts at:      0    1406    1533 
   Size:       1406     127
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     360     393    1533 
   Size:        360      33    1140

  Fragments arranged by size: 

                1140     360      33
 
BanI G'GyrC_C

Cuts at:      0     860    1533 
   Size:        860     673
 
BanII G_rGCy'C

Cuts at:      0      11     840    1533 
   Size:         11     829     693

  Fragments arranged by size: 

                 829     693      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     733    1533 
   Size:        733     800
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     593    1334    1533 
   Size:        593     741     199

  Fragments arranged by size: 

                 741     593     199
 
BsaHI Gr'CG_yC

Cuts at:      0    1448    1533 
   Size:       1448      85
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1444    1474    1533 
   Size:       1444      30      59

  Fragments arranged by size: 

                1444      59      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1001    1533 
   Size:       1001     532
 
BseYI C'CCAG_C

Cuts at:      0    1297    1533 
   Size:       1297     236
 
BsiEI CG_ry'CG

Cuts at:      0      41    1533 
   Size:         41    1492
 
BsiHKAI G_wGCw'C

Cuts at:      0    1238    1533 
   Size:       1238     295
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     840    1238    1533 
   Size:         11     829     398     295

  Fragments arranged by size: 

                 829     398     295      11
 
BspHI T'CATG_A

Cuts at:      0     450    1533 
   Size:        450    1083
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     672    1533 
   Size:        672     861
 
BsrDI GCAATG_nn'

Cuts at:      0    1512    1533 
   Size:       1512      21
 
BsrFI r'CCGG_y

Cuts at:      0      80    1533 
   Size:         80    1453
 
BsrGI T'GTAC_A

Cuts at:      0     869    1533 
   Size:        869     664
 
BssSI C'ACGA_G

Cuts at:      0     122    1533 
   Size:        122    1411
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1165    1329    1533 
   Size:       1165     164     204

  Fragments arranged by size: 

                1165     204     164
 
BstYI r'GATC_y

Cuts at:      0     316    1196    1533 
   Size:        316     880     337

  Fragments arranged by size: 

                 880     337     316
 
BstZ17I GTA'TAC

Cuts at:      0     942    1533 
   Size:        942     591
 
DraI TTT'AAA

Cuts at:      0     486    1533 
   Size:        486    1047
 
EaeI y'GGCC_r

Cuts at:      0    1217    1533 
   Size:       1217     316
 
EarI CTCTTCn'nnn_

Cuts at:      0     164     346    1238    1533 
   Size:        164     182     892     295

  Fragments arranged by size: 

                 892     295     182     164
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     593    1334    1533 
   Size:        357     236     741     199

  Fragments arranged by size: 

                 741     357     236     199
 
EcoRI G'AATT_C

Cuts at:      0     799    1533 
   Size:        799     734
 
EcoRV GAT'ATC

Cuts at:      0     132     633     687    1533 
   Size:        132     501      54     846

  Fragments arranged by size: 

                 846     501     132      54
 
HaeII r_GCGC'y

Cuts at:      0    1340    1533 
   Size:       1340     193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222    1407    1439    1533 
   Size:        190      32    1185      32      94

  Fragments arranged by size: 

                1185     190      94      32      32
 
HincII GTy'rAC

Cuts at:      0      35     360    1533 
   Size:         35     325    1173

  Fragments arranged by size: 

                1173     325      35
 
KpnI G_GTAC'C

Cuts at:      0     864    1533 
   Size:        864     669
 
MfeI C'AATT_G

Cuts at:      0      17    1533 
   Size:         17    1516
 
MluI A'CGCG_T

Cuts at:      0     516    1533 
   Size:        516    1017
 
MscI TGG'CCA

Cuts at:      0    1219    1533 
   Size:       1219     314
 
MspA1I CmG'CkG

Cuts at:      0    1165    1301    1533 
   Size:       1165     136     232

  Fragments arranged by size: 

                1165     232     136
 
NdeI CA'TA_TG

Cuts at:      0     669    1533 
   Size:        669     864
 
NspI r_CATG'y

Cuts at:      0     870    1293    1533 
   Size:        870     423     240

  Fragments arranged by size: 

                 870     423     240
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1069    1533 
   Size:       1069     464
 
PstI C_TGCA'G

Cuts at:      0     960    1170    1533 
   Size:        960     210     363

  Fragments arranged by size: 

                 960     363     210
 
PvuII CAG'CTG

Cuts at:      0    1165    1533 
   Size:       1165     368
 
SalI G'TCGA_C

Cuts at:      0      33    1533 
   Size:         33    1500
 
ScaI AGT'ACT

Cuts at:      0    1307    1533 
   Size:       1307     226
 
SfcI C'TryA_G

Cuts at:      0     777     956    1166    1533 
   Size:        777     179     210     367

  Fragments arranged by size: 

                 777     367     210     179
 
SphI G_CATG'C

Cuts at:      0    1293    1533 
   Size:       1293     240
 
SspI AAT'ATT

Cuts at:      0    1527    1533 
   Size:       1527       6
 
StyI C'CwwG_G

Cuts at:      0      11    1388    1533 
   Size:         11    1377     145

  Fragments arranged by size: 

                1377     145      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55     662    1415    1533 
   Size:         55     607     753     118

  Fragments arranged by size: 

                 753     607     118      55
 
TatI w'GTAC_w

Cuts at:      0     869    1305    1533 
   Size:        869     436     228

  Fragments arranged by size: 

                 869     436     228
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII    AlwNI     ApoI
     AseI     BaeI     BanI    BanII     BbsI     BpmI    BsaHI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstYI  BstZ17I     DraI     EaeI
     EarI  Eco57MI    EcoRI    EcoRV    HaeII    Hin4I   HincII     KpnI
     MfeI     MluI     MscI   MspA1I     NdeI     NspI    PflMI     PstI
    PvuII     SalI     ScaI     SfcI     SphI     SspI     StyI    TaqII
     TatI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AhdI     AleI     AlfI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI   BssHII    BstBI   BstEII    BstXI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     MmeI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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