Restriction map for pF1KB9499
 (Linear) (Six-base) MAPSORT of: KIBB9499.seq  Check: 7606  from: 1  to: 3402



KIBB9499.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2112    3402 
   Size:       2112    1290
 
AatII G_ACGT'C

Cuts at:      0    2368    3402 
   Size:       2368    1034
 
AccI GT'mk_AC

Cuts at:      0    1807    3010    3402 
   Size:       1807    1203     392

  Fragments arranged by size: 

                1807    1203     392
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     545    1342    3156    3402 
   Size:        217     328     797    1814     246

  Fragments arranged by size: 

                1814     797     328     246     217
 
AfeI AGC'GCT

Cuts at:      0    3177    3402 
   Size:       3177     225
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1381    3402 
   Size:       1381    2021
 
AlwNI CAG_nnn'CTG

Cuts at:      0     130     523     834    1605    2229    2392    3229    3348 
   Size:        130     393     311     771     624     163     837     119

Cuts at:   3348    3402 
   Size:         54

  Fragments arranged by size: 

                 837     771     624     393     311     163     130     119
                  54
 
ApaI G_GGCC'C

Cuts at:      0    2378    2488    3402 
   Size:       2378     110     914

  Fragments arranged by size: 

                2378     914     110
 
ApoI r'AATT_y

Cuts at:      0     319     751    1036    2074    3402 
   Size:        319     432     285    1038    1328

  Fragments arranged by size: 

                1328    1038     432     319     285
 
AseI AT'TA_AT

Cuts at:      0    1163    3402 
   Size:       1163    2239
 
AvaI C'yCGr_G

Cuts at:      0     489    1196    1908    2757    3235    3402 
   Size:        489     707     712     849     478     167

  Fragments arranged by size: 

                 849     712     707     489     478     167
 
BanI G'GyrC_C

Cuts at:      0     850    1739    3324    3402 
   Size:        850     889    1585      78

  Fragments arranged by size: 

                1585     889     850      78
 
BanII G_rGCy'C

Cuts at:      0      11    1922    2378    2488    3118    3402 
   Size:         11    1911     456     110     630     284

  Fragments arranged by size: 

                1911     630     456     284     110      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1994    2542    3259    3402 
   Size:       1994     548     717     143

  Fragments arranged by size: 

                1994     717     548     143
 
BbvCI CC'TCA_GC

Cuts at:      0    1124    3402 
   Size:       1124    2278
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1680    3402 
   Size:       1680    1722
 
BglII A'GATC_T

Cuts at:      0     255    3402 
   Size:        255    3147
 
BlpI GC'TnA_GC

Cuts at:      0    1204    2342    3402 
   Size:       1204    1138    1060

  Fragments arranged by size: 

                1204    1138    1060
 
Bme1580I G_kGCm'C

Cuts at:      0    1742    2378    2488    3329    3402 
   Size:       1742     636     110     841      73

  Fragments arranged by size: 

                1742     841     636     110      73
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2245    3402 
   Size:       2245    1157
 
BmtI G_CTAG'C

Cuts at:      0    2189    3402 
   Size:       2189    1213
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     870    3402 
   Size:        870    2532
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     865     889     953    1102    1318    1612    1627 
   Size:        260     605      24      64     149     216     294      15

Cuts at:   1627    1990    2464    2586    2891    3270    3402 
   Size:        363     474     122     305     379     132

  Fragments arranged by size: 

                 605     474     379     363     305     294     260     216
                 149     132     122      64      24      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     827    1106    1124    2647    3402 
   Size:        827     279      18    1523     755

  Fragments arranged by size: 

                1523     827     755     279      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1261    1327    3402 
   Size:       1261      66    2075

  Fragments arranged by size: 

                2075    1261      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3207    3402 
   Size:       3207     195
 
BsaHI Gr'CG_yC

Cuts at:      0    2365    3402 
   Size:       2365    1037
 
BsaWI w'CCGG_w

Cuts at:      0    1466    3402 
   Size:       1466    1936
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1552    1582    3402 
   Size:       1552      30    1820

  Fragments arranged by size: 

                1820    1552      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     802     805    1456    1946    2001    3048    3174 
   Size:         94     708       3     651     490      55    1047     126

Cuts at:   3174    3402 
   Size:        228

  Fragments arranged by size: 

                1047     708     651     490     228     126      94      55
                   3
 
BseYI C'CCAG_C

Cuts at:      0      85    2062    2625    3402 
   Size:         85    1977     563     777

  Fragments arranged by size: 

                1977     777     563      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2072    2555    3402 
   Size:       2072     483     847

  Fragments arranged by size: 

                2072     847     483
 
BsiHKAI G_wGCw'C

Cuts at:      0    1287    1922    3118    3167    3402 
   Size:       1287     635    1196      49     235

  Fragments arranged by size: 

                1287    1196     635     235      49
 
BsmI GAATG_Cn'

Cuts at:      0    1402    3402 
   Size:       1402    2000
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2372    3402 
   Size:       2372    1030
 
Bsp1286I G_dGCh'C

Cuts at:      0      11    1287    1742    1922    2378    2488    3118    3167 
   Size:         11    1276     455     180     456     110     630      49

Cuts at:   3167    3329    3402 
   Size:        162      73

  Fragments arranged by size: 

                1276     630     456     455     180     162     110      73
                  49      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2112    3402 
   Size:       2112    1290
 
BsrBI CCG'CTC

Cuts at:      0    1220    3402 
   Size:       1220    2182
 
BsrDI GCAATG_nn'

Cuts at:      0    1859    3402 
   Size:       1859    1543
 
BsrFI r'CCGG_y

Cuts at:      0     117     667    2425    3052    3402 
   Size:        117     550    1758     627     350

  Fragments arranged by size: 

                1758     627     550     350     117
 
BsrGI T'GTAC_A

Cuts at:      0    2131    3402 
   Size:       2131    1271
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1423    3348    3402 
   Size:       1423    1925      54

  Fragments arranged by size: 

                1925    1423      54
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     921    1434    2037    3256    3402 
   Size:        921     513     603    1219     146

  Fragments arranged by size: 

                1219     921     603     513     146
 
BstYI r'GATC_y

Cuts at:      0     255     264    3402 
   Size:        255       9    3138

  Fragments arranged by size: 

                3138     255       9
 
Bsu36I CC'TnA_GG

Cuts at:      0    2583    3402 
   Size:       2583     819
 
BtgI C'CryG_G

Cuts at:      0     920    1734    2359    2822    3249    3391    3402 
   Size:        920     814     625     463     427     142      11

  Fragments arranged by size: 

                 920     814     625     463     427     142      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1378    3402 
   Size:       1378    2024
 
BtsI GCAGTG_nn'

Cuts at:      0     914    2080    3402 
   Size:        914    1166    1322

  Fragments arranged by size: 

                1322    1166     914
 
ClaI AT'CG_AT

Cuts at:      0     377     416    3402 
   Size:        377      39    2986

  Fragments arranged by size: 

                2986     377      39
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3380    3402 
   Size:       3380      22
 
DraIII CAC_nnn'GTG

Cuts at:      0    2507    3402 
   Size:       2507     895
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2970    3402 
   Size:       2970     432
 
EaeI y'GGCC_r

Cuts at:      0     911    1379    1757    2543    3050    3258    3402 
   Size:        911     468     378     786     507     208     144

  Fragments arranged by size: 

                 911     786     507     468     378     208     144
 
EarI CTCTTCn'nnn_

Cuts at:      0    1056    1433    2643    2757    3402 
   Size:       1056     377    1210     114     645

  Fragments arranged by size: 

                1210    1056     645     377     114
 
EcoICRI GAG'CTC

Cuts at:      0    1920    3116    3402 
   Size:       1920    1196     286

  Fragments arranged by size: 

                1920    1196     286
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     260     545     865     889     953    1102    1318 
   Size:        217      43     285     320      24      64     149     216

Cuts at:   1318    1342    1612    1627    1990    2464    2586    2891    3156 
   Size:         24     270      15     363     474     122     305     265

Cuts at:   3156    3270    3402 
   Size:        114     132

  Fragments arranged by size: 

                 474     363     320     305     285     270     265     217
                 216     149     132     122     114      64      43      24
                  24      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1911    2308    3402 
   Size:       1911     397    1094

  Fragments arranged by size: 

                1911    1094     397
 
EcoO109I rG'GnC_Cy

Cuts at:      0     306     831    1522    1602    1904    2313    2375    2484 
   Size:        306     525     691      80     302     409      62     109

Cuts at:   2484    2939    3226    3239    3379    3402 
   Size:        455     287      13     140      23

  Fragments arranged by size: 

                 691     525     455     409     306     302     287     140
                 109      80      62      23      13
 
EcoRI G'AATT_C

Cuts at:      0    1036    2074    3402 
   Size:       1036    1038    1328

  Fragments arranged by size: 

                1328    1038    1036
 
HaeII r_GCGC'y

Cuts at:      0    3179    3307    3402 
   Size:       3179     128      95

  Fragments arranged by size: 

                3179     128      95
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     625     657    1909    1941    1986    2018    3402 
   Size:        625      32    1252      32      45      32    1384

  Fragments arranged by size: 

                1384    1252     625      45      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1965    2891    3402 
   Size:       1965     926     511

  Fragments arranged by size: 

                1965     926     511
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     428    2097    3402 
   Size:        428    1669    1305

  Fragments arranged by size: 

                1669    1305     428
 
MscI TGG'CCA

Cuts at:      0    1381    1759    3260    3402 
   Size:       1381     378    1501     142

  Fragments arranged by size: 

                1501    1381     378     142
 
MslI CAynn'nnrTG

Cuts at:      0     919    1301    3402 
   Size:        919     382    2101

  Fragments arranged by size: 

                2101     919     382
 
MspA1I CmG'CkG

Cuts at:      0     130     820    2062    2542    3402 
   Size:        130     690    1242     480     860

  Fragments arranged by size: 

                1242     860     690     480     130
 
NaeI GCC'GGC

Cuts at:      0     119     669    2427    3054    3402 
   Size:        119     550    1758     627     348

  Fragments arranged by size: 

                1758     627     550     348     119
 
NcoI C'CATG_G

Cuts at:      0     920    1734    3249    3402 
   Size:        920     814    1515     153

  Fragments arranged by size: 

                1515     920     814     153
 
NgoMIV G'CCGG_C

Cuts at:      0     117     667    2425    3052    3402 
   Size:        117     550    1758     627     350

  Fragments arranged by size: 

                1758     627     550     350     117
 
NheI G'CTAG_C

Cuts at:      0    2185    3402 
   Size:       2185    1217
 
PasI CC'CwG_GG

Cuts at:      0    2943    3402 
   Size:       2943     459
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1605    1691    2949    3402 
   Size:       1605      86    1258     453

  Fragments arranged by size: 

                1605    1258     453      86
 
PfoI T'CCnGG_A

Cuts at:      0      70    3402 
   Size:         70    3332
 
PpuMI rG'GwC_Cy

Cuts at:      0    1522    1602    2939    3239    3402 
   Size:       1522      80    1337     300     163

  Fragments arranged by size: 

                1522    1337     300     163      80
 
PsiI TTA'TAA

Cuts at:      0    3136    3402 
   Size:       3136     266
 
PspOMI G'GGCC_C

Cuts at:      0    2374    2484    3402 
   Size:       2374     110     918

  Fragments arranged by size: 

                2374     918     110
 
PspXI vC'TCGA_Gb

Cuts at:      0    1908    3402 
   Size:       1908    1494
 
PstI C_TGCA'G

Cuts at:      0     126     503    1261    2109    2198    2528    2685    2991 
   Size:        126     377     758     848      89     330     157     306

Cuts at:   2991    3402 
   Size:        411

  Fragments arranged by size: 

                 848     758     411     377     330     306     157     126
                  89
 
PvuII CAG'CTG

Cuts at:      0     130    2062    3402 
   Size:        130    1932    1340

  Fragments arranged by size: 

                1932    1340     130
 
SacI G_AGCT'C

Cuts at:      0    1922    3118    3402 
   Size:       1922    1196     284

  Fragments arranged by size: 

                1922    1196     284
 
SanDI GG'GwC_CC

Cuts at:      0    2939    3402 
   Size:       2939     463
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2757    3402 
   Size:       2757     645
 
SfcI C'TryA_G

Cuts at:      0     122     499    1257    2105    2194    2524    2681    2987 
   Size:        122     377     758     848      89     330     157     306

Cuts at:   2987    3402 
   Size:        415

  Fragments arranged by size: 

                 848     758     415     377     330     306     157     122
                  89
 
SmaI CCC'GGG

Cuts at:      0    3237    3402 
   Size:       3237     165
 
SmlI C'TyrA_G

Cuts at:      0    1276    1342    1908    3402 
   Size:       1276      66     566    1494

  Fragments arranged by size: 

                1494    1276     566      66
 
SspI AAT'ATT

Cuts at:      0     715    3402 
   Size:        715    2687
 
StuI AGG'CCT

Cuts at:      0     230    1067    1892    2591    3402 
   Size:        230     837     825     699     811

  Fragments arranged by size: 

                 837     825     811     699     230
 
StyI C'CwwG_G

Cuts at:      0     920    1014    1734    2030    3125    3206    3249    3255 
   Size:        920      94     720     296    1095      81      43       6

Cuts at:   3255    3402 
   Size:        147

  Fragments arranged by size: 

                1095     920     720     296     147      94      81      43
                   6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     374    1168    3346    3402 
   Size:        374     794    2178      56

  Fragments arranged by size: 

                2178     794     374      56
 
TatI w'GTAC_w

Cuts at:      0    2131    2325    3402 
   Size:       2131     194    1077

  Fragments arranged by size: 

                2131    1077     194
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1552    1584    3402 
   Size:       1552      32    1818

  Fragments arranged by size: 

                1818    1552      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3006    3402 
   Size:       3006     396
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1162    3253    3402 
   Size:       1162    2091     149

  Fragments arranged by size: 

                2091    1162     149
 
XhoI C'TCGA_G

Cuts at:      0    1908    3402 
   Size:       1908    1494
 
XmaI C'CCGG_G

Cuts at:      0    3235    3402 
   Size:       3235     167
 
XmnI GAAnn'nnTTC

Cuts at:      0    1008    1147    1534    2446    3402 
   Size:       1008     139     387     912     956

  Fragments arranged by size: 

                1008     956     912     387     139
 
ZraI GAC'GTC

Cuts at:      0    2366    3402 
   Size:       2366    1036
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI     AlfI    AlwNI     ApaI
     ApoI     AseI     AvaI     BanI    BanII     BbsI    BbvCI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    HaeII    Hin4I   HincII     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI     PasI    PflMI
     PfoI    PpuMI     PsiI   PspOMI    PspXI     PstI    PvuII     SacI
    SanDI     SapI     SfcI     SmaI     SmlI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI     AloI
    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI
    BciVI     BclI    BfrBI    BmgBI    BsaAI    BsaBI    BsiEI    BsiWI
    BspEI    BspHI   BssHII    BssSI    BstBI   BstEII  BstZ17I     DraI
     EagI     EciI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SwaI     XbaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com