Restriction map for pF1KB9498
 (Linear) (Six-base) MAPSORT of: KIBB9498.seq  Check: 7244  from: 1  to: 1833



KIBB9498.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AgeI A'CCGG_T

Cuts at:      0     692    1833 
   Size:        692    1141
 
ApoI r'AATT_y

Cuts at:      0     340     427     494    1833 
   Size:        340      87      67    1339

  Fragments arranged by size: 

                1339     340      87      67
 
AvaI C'yCGr_G

Cuts at:      0     239     950    1064    1199    1833 
   Size:        239     711     114     135     634

  Fragments arranged by size: 

                 711     634     239     135     114
 
BanI G'GyrC_C

Cuts at:      0     192    1833 
   Size:        192    1641
 
BanII G_rGCy'C

Cuts at:      0     603     934    1492    1833 
   Size:        603     331     558     341

  Fragments arranged by size: 

                 603     558     341     331
 
BbeI G_GCGC'C

Cuts at:      0     196    1833 
   Size:        196    1637
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      43     259     902    1833 
   Size:         43     216     643     931

  Fragments arranged by size: 

                 931     643     216      43
 
BbvCI CC'TCA_GC

Cuts at:      0       9      65    1833 
   Size:          9      56    1768

  Fragments arranged by size: 

                1768      56       9
 
BclI T'GATC_A

Cuts at:      0     513    1401    1833 
   Size:        513     888     432

  Fragments arranged by size: 

                 888     513     432
 
BglII A'GATC_T

Cuts at:      0    1711    1833 
   Size:       1711     122
 
Bme1580I G_kGCm'C

Cuts at:      0     459    1833 
   Size:        459    1374
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1050    1289    1628    1833 
   Size:       1050     239     339     205

  Fragments arranged by size: 

                1050     339     239     205
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     615     918    1523    1692    1833 
   Size:        615     303     605     169     141

  Fragments arranged by size: 

                 615     605     303     169     141
 
Bpu10I CC'TnA_GC

Cuts at:      0       9      65    1721    1833 
   Size:          9      56    1656     112

  Fragments arranged by size: 

                1656     112      56       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1652    1833 
   Size:       1652     181
 
BsaAI yAC'GTr

Cuts at:      0     382    1833 
   Size:        382    1451
 
BsaBI GATnn'nnATC

Cuts at:      0     563     707     719     746    1833 
   Size:        563     144      12      27    1087

  Fragments arranged by size: 

                1087     563     144      27      12
 
BsaHI Gr'CG_yC

Cuts at:      0     193    1833 
   Size:        193    1640
 
BsaWI w'CCGG_w

Cuts at:      0     257     692    1833 
   Size:        257     435    1141

  Fragments arranged by size: 

                1141     435     257
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     588     618    1308    1338    1833 
   Size:        588      30     690      30     495

  Fragments arranged by size: 

                 690     588     495      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     890    1833 
   Size:        890     943
 
BseYI C'CCAG_C

Cuts at:      0     116     388    1833 
   Size:        116     272    1445

  Fragments arranged by size: 

                1445     272     116
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386    1700    1833 
   Size:        386    1314     133

  Fragments arranged by size: 

                1314     386     133
 
BsiEI CG_ry'CG

Cuts at:      0     258     925    1252    1833 
   Size:        258     667     327     581

  Fragments arranged by size: 

                 667     581     327     258
 
BsiHKAI G_wGCw'C

Cuts at:      0     934    1492    1833 
   Size:        934     558     341

  Fragments arranged by size: 

                 934     558     341
 
BsmI GAATG_Cn'

Cuts at:      0    1449    1605    1833 
   Size:       1449     156     228

  Fragments arranged by size: 

                1449     228     156
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1637    1833 
   Size:       1637     196
 
Bsp1286I G_dGCh'C

Cuts at:      0     459     603     934    1492    1833 
   Size:        459     144     331     558     341

  Fragments arranged by size: 

                 558     459     341     331     144
 
BsrFI r'CCGG_y

Cuts at:      0     692    1833 
   Size:        692    1141
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     116    1833 
   Size:        116    1717
 
BstYI r'GATC_y

Cuts at:      0    1711    1833 
   Size:       1711     122
 
Bsu36I CC'TnA_GG

Cuts at:      0    1025    1736    1833 
   Size:       1025     711      97

  Fragments arranged by size: 

                1025     711      97
 
BtgI C'CryG_G

Cuts at:      0     229    1632    1833 
   Size:        229    1403     201

  Fragments arranged by size: 

                1403     229     201
 
BtsI GCAGTG_nn'

Cuts at:      0     221     287     374     831     852    1833 
   Size:        221      66      87     457      21     981

  Fragments arranged by size: 

                 981     457     221      87      66      21
 
ClaI AT'CG_AT

Cuts at:      0     750    1833 
   Size:        750    1083
 
EaeI y'GGCC_r

Cuts at:      0     552     759    1833 
   Size:        552     207    1074

  Fragments arranged by size: 

                1074     552     207
 
EarI CTCTTCn'nnn_

Cuts at:      0     320     467     636     828    1833 
   Size:        320     147     169     192    1005

  Fragments arranged by size: 

                1005     320     192     169     147
 
EcoICRI GAG'CTC

Cuts at:      0     932    1490    1833 
   Size:        932     558     343

  Fragments arranged by size: 

                 932     558     343
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     615     918    1523    1692    1833 
   Size:        615     303     605     169     141

  Fragments arranged by size: 

                 615     605     303     169     141
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     641    1833 
   Size:        641    1192
 
EcoO109I rG'GnC_Cy

Cuts at:      0     435     979    1283    1833 
   Size:        435     544     304     550

  Fragments arranged by size: 

                 550     544     435     304
 
EcoRI G'AATT_C

Cuts at:      0     340     494    1833 
   Size:        340     154    1339

  Fragments arranged by size: 

                1339     340     154
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     360    1833 
   Size:        360    1473
 
HaeII r_GCGC'y

Cuts at:      0     196    1833 
   Size:        196    1637
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     344     376     588     620     907     931     939     963 
   Size:        344      32     212      32     287      24       8      24

Cuts at:    963    1024    1056    1357    1389    1492    1524    1690    1696 
   Size:         61      32     301      32     103      32     166       6

Cuts at:   1696    1722    1728    1833 
   Size:         26       6     105

  Fragments arranged by size: 

                 344     301     287     212     166     105     103      61
                  32      32      32      32      32      26      24      24
                   8       6       6
 
HincII GTy'rAC

Cuts at:      0    1101    1833 
   Size:       1101     732
 
KasI G'GCGC_C

Cuts at:      0     192    1833 
   Size:        192    1641
 
MfeI C'AATT_G

Cuts at:      0     200    1833 
   Size:        200    1633
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1402    1833 
   Size:       1402     431
 
MspA1I CmG'CkG

Cuts at:      0     116    1108    1170    1833 
   Size:        116     992      62     663

  Fragments arranged by size: 

                 992     663     116      62
 
NarI GG'CG_CC

Cuts at:      0     193    1833 
   Size:        193    1640
 
PfoI T'CCnGG_A

Cuts at:      0     814    1638    1833 
   Size:        814     824     195

  Fragments arranged by size: 

                 824     814     195
 
PmlI CAC'GTG

Cuts at:      0     382    1833 
   Size:        382    1451
 
PpuMI rG'GwC_Cy

Cuts at:      0     435     979    1283    1833 
   Size:        435     544     304     550

  Fragments arranged by size: 

                 550     544     435     304
 
PspXI vC'TCGA_Gb

Cuts at:      0    1064    1833 
   Size:       1064     769
 
PvuII CAG'CTG

Cuts at:      0     116    1108    1170    1833 
   Size:        116     992      62     663

  Fragments arranged by size: 

                 992     663     116      62
 
SacI G_AGCT'C

Cuts at:      0     934    1492    1833 
   Size:        934     558     341

  Fragments arranged by size: 

                 934     558     341
 
SapI GCTCTTCn'nnn_

Cuts at:      0     828    1833 
   Size:        828    1005
 
ScaI AGT'ACT

Cuts at:      0    1020    1833 
   Size:       1020     813
 
SfcI C'TryA_G

Cuts at:      0     611     870    1138    1833 
   Size:        611     259     268     695

  Fragments arranged by size: 

                 695     611     268     259
 
SfoI GGC'GCC

Cuts at:      0     194    1833 
   Size:        194    1639
 
SmlI C'TyrA_G

Cuts at:      0    1064    1833 
   Size:       1064     769
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     794     911     914    1226    1440    1833 
   Size:        794     117       3     312     214     393

  Fragments arranged by size: 

                 794     393     312     214     117       3
 
TatI w'GTAC_w

Cuts at:      0    1018    1833 
   Size:       1018     815
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     810     842    1248    1280    1308    1340    1833 
   Size:        810      32     406      32      28      32     493

  Fragments arranged by size: 

                 810     493     406      32      32      32      28
 
XbaI T'CTAG_A

Cuts at:      0    1335    1833 
   Size:       1335     498
 
XhoI C'TCGA_G

Cuts at:      0    1064    1833 
   Size:       1064     769
 
XmnI GAAnn'nnTTC

Cuts at:      0     344    1833 
   Size:        344    1489
 

 Enzymes that do cut:

     AgeI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BclI    BglII Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrFI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     ClaI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII     KasI     MfeI
     MmeI   MspA1I     NarI     PfoI     PmlI    PpuMI    PspXI    PvuII
     SacI     SapI     ScaI     SfcI     SfoI     SmlI    TaqII     TatI
     TstI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI
    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI    BpuEI    BsiWI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MluI     MscI     MslI     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI  Tth111I     XcmI     XmaI     ZraI
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