Restriction map for pF1KB9497
##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AflIII      
ApoI        AscI        AseI        AsnI        AvrII       
BbrPI       BfrI        BlnI        BsaAI       BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BssHII      
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EcoRI       EcoRV       EcoT22I     
FseI        HincII      HindII      HpaI        KspI        
MfeI        MluI        MroI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SfiI        SfuI        SnaBI       SpeI        SphI        
SspI        SwaI        TfiI        Tsp509I     XbaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII         571  GACGTC               GACGT^C
Acc65I        408  GGTACC               G^GTACC
AccI         1722  GTCTAC               GT^[AC][GT]AC
AhdI         1148  GACCGCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1422  GTGCAC               G^TGCAC
AocI         3519  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1422  GTGCAC               G^TGCAC
Asp718I       408  GGTACC               G^GTACC
AspEI        1148  GACCGCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1481  TGCGCA               TGC^GCA
BciVI         429  GGATAC               GTATCC(6/5)
BglII        2127  AGATCT               A^GATCT
BsaBI        1353  GATTGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        3202  CGGCCG               CG[GA][CT]^CG
BsmBI        2615  GAGACG               CGTCTC(1/5)
BsrDI        1825  GCAATG               GCAATG(2/0)
BssSI          22  CTCGTG               CACGAG(-5/-1)
BstAPI       1460  GCATTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       3519  CCTGAGG              CC^T[ACGT]AGG
DraIII        151  CACGTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         3202  CGGCCG               C^GGCCG
Eam1105I     1148  GACCGCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3202  CGGCCG               C^GGCCG
Eco52I       3202  CGGCCG               C^GGCCG
Eco81I       3519  CCTGAGG              CC^T[ACGT]AGG
FspI         1481  TGCGCA               TGC^GCA
HindIII      2587  AAGCTT               A^AGCTT
KpnI          408  GGTACC               GGTAC^C
MamI         1353  GATTGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         2393  TTAA                 T^TAA
NspI         2606  GCATGT               [GA]CATG^[CT]
PshAI        1773  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         3288  CCTGCAGG             CCTGCA^GG
ScaI         2594  AGTACT               AGT^ACT
SgrAI        1090  CACCGGCG             C[GA]^CCGG[CT]G
Sse8387I     3288  CCTGCAGG             CCTGCA^GG
Tru9I        2393  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII         729  CGCG                 CG^CG
             2218  CGCG                
AgeI           40  ACCGGT               A^CCGGT
              654  ACCGGT              
Asp700I      2487  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3453  GAAGCGCTTC          
BamHI         176  GGATCC               G^GATCC
             2397  GGATCC              
BbeI         2620  GGCGCC               GGCGC^C
             3189  GGCGCC              
BcgI          461  CGAAGCGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               14  GCAGCCCTCTCG        
BclI          644  TGATCA               T^GATCA
             2303  TGATCA              
BsaWI          40  ACCGGT               [AT]^CCGG[AT]
              654  ACCGGT              
BsmI         1031  GCATTC               GAATGC(1/-1)
             1588  GCATTC              
Bsp1407I      809  TGTACA               T^GTACA
             2801  TGTACA              
BspHI        2153  TCATGA               T^CATGA
             2915  TCATGA              
BsrGI         809  TGTACA               T^GTACA
             2801  TGTACA              
BstUI         729  CGCG                 CG^CG
             2218  CGCG                
FbaI          644  TGATCA               T^GATCA
             2303  TGATCA              
HgaI         1853  GCGTC                GACGC(5/10)
             2780  GCGTC               
KasI         2620  GGCGCC               G^GCGCC
             3189  GGCGCC              
MvnI          729  CGCG                 CG^CG
             2218  CGCG                
NarI         2620  GGCGCC               GG^CGCC
             3189  GGCGCC              
PaeR7I       2350  CTCGAG               C^TCGAG
             2706  CTCGAG              
PflMI         672  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1776  CCAGGTCTTGG         
PinAI          40  ACCGGT               A^CCGGT
              654  ACCGGT              
RcaI         2153  TCATGA               T^CATGA
             2915  TCATGA              
SfoI         2620  GGCGCC               GGC^GCC
             3189  GGCGCC              
SmaI         1133  CCCGGG               CCC^GGG
             2267  CCCGGG              
SmlI         2350  CTCGAG               C^T[CT][GA]AG
             2706  CTCGAG              
SspBI         809  TGTACA               T^GTACA
             2801  TGTACA              
StuI          716  AGGCCT               AGG^CCT
             1934  AGGCCT              
Van91I        672  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1776  CCAGGTCTTGG         
XhoI         2350  CTCGAG               C^TCGAG
             2706  CTCGAG              
XmaCI        1133  CCCGGG               C^CCGGG
             2267  CCCGGG              
XmaI         1133  CCCGGG               C^CCGGG
             2267  CCCGGG              
XmnI         2487  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3453  GAAGCGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       Aor51HI     ApaI        AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       PflFI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
Tth111I     TthHB8I     XcmI        XhoII       
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