Restriction map for
pF1KB9497
(Circular) (Six-base) MAPSORT of: pF1KB9497.seq Check: 295 from: 1 to: 6638
pF1KB9497 6638 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1512 1771 1512
Size: 259 6379
AatII G_ACGT'C
Cuts at: 683 683
Size: 6638
AccI GT'mk_AC
Cuts at: 1831 3676 1831
Size: 1845 4793
Acc65I G'GTAC_C
Cuts at: 516 3654 516
Size: 3138 3500
AclI AA'CG_TT
Cuts at: 6478 6478
Size: 6638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 1171 1705 2296 2683 2770 4463 4895 5248
Size: 358 534 591 387 87 1693 432 353
Cuts at: 5248 5910 813
Size: 662 1541
Fragments arranged by size:
1693 1541 662 591 534 432 387 358
353 87
AfeI AGC'GCT
Cuts at: 607 3044 3565 607
Size: 2437 521 3680
Fragments arranged by size:
3680 2437 521
AflIII A'CryG_T
Cuts at: 3990 5790 6071 3990
Size: 1800 281 4557
Fragments arranged by size:
4557 1800 281
AgeI A'CCGG_T
Cuts at: 148 762 148
Size: 614 6024
AhdI GACnn_n'nnGTC
Cuts at: 1261 1261
Size: 6638
AleI CACnn'nnGTG
Cuts at: 1795 2488 3010 3325 1795
Size: 693 522 315 5108
Fragments arranged by size:
5108 693 522 315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1162 2097 2191 3124 1162
Size: 935 94 933 4676
Fragments arranged by size:
4676 935 933 94
AlwNI CAG_nnn'CTG
Cuts at: 786 2121 2140 2211 2307 2982 3943 5381 786
Size: 1335 19 71 96 675 961 1438 2043
Fragments arranged by size:
2043 1438 1335 961 675 96 71 19
ApaI G_GGCC'C
Cuts at: 1181 1742 2576 2748 1181
Size: 561 834 172 5071
Fragments arranged by size:
5071 834 561 172
ApaLI G'TGCA_C
Cuts at: 1530 5476 1530
Size: 3946 2692
ApoI r'AATT_y
Cuts at: 3642 5991 6247 3642
Size: 2349 256 4033
Fragments arranged by size:
4033 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6638
AvaI C'yCGr_G
Cuts at: 1241 2038 2089 2375 2458 2814 3658 1241
Size: 797 51 286 83 356 844 4221
Fragments arranged by size:
4221 844 797 356 286 83 51
BamHI G'GATC_C
Cuts at: 284 2505 3663 284
Size: 2221 1158 3259
Fragments arranged by size:
3259 2221 1158
BanI G'GyrC_C
Cuts at: 516 526 1408 1446 1497 1639 1677 1950 2555
Size: 10 882 38 51 142 38 273 605
Cuts at: 2555 2728 3297 3654 4068 4316 4351 516
Size: 173 569 357 414 248 35 2803
Fragments arranged by size:
2803 882 605 569 414 357 273 248
173 142 51 38 38 35 10
BanII G_rGCy'C
Cuts at: 1123 1181 1321 1476 1742 1922 1946 2081 2576
Size: 58 140 155 266 180 24 135 495
Cuts at: 2576 2748 3361 3652 4682 1123
Size: 172 613 291 1030 3079
Fragments arranged by size:
3079 1030 613 495 291 266 180 172
155 140 135 58 24
BbeI G_GCGC'C
Cuts at: 2732 3301 4072 4320 2732
Size: 569 771 248 5050
Fragments arranged by size:
5050 771 569 248
BbsI GAAGACnn'nnnn_
Cuts at: 1159 2674 2883 3234 1159
Size: 1515 209 351 4563
Fragments arranged by size:
4563 1515 351 209
BbvCI CC'TCA_GC
Cuts at: 190 703 1774 2566 3415 190
Size: 513 1071 792 849 3413
Fragments arranged by size:
3413 1071 849 792 513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 111 145 558 592 111
Size: 34 413 34 6157
Fragments arranged by size:
6157 413 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 531 4536 5592 6638 531
Size: 4005 1056 1046 531
Fragments arranged by size:
4005 1056 1046 531
BclI T'GATC_A
Cuts at: 752 2411 4158 752
Size: 1659 1747 3232
Fragments arranged by size:
3232 1747 1659
BglI GCCn_nnn'nGGC
Cuts at: 1093 1170 1737 2653 2752 1093
Size: 77 567 916 99 4979
Fragments arranged by size:
4979 916 567 99 77
BglII A'GATC_T
Cuts at: 2235 4153 6190 2235
Size: 1918 2037 2683
Fragments arranged by size:
2683 2037 1918
BlpI GC'TnA_GC
Cuts at: 217 3082 3097 3750 217
Size: 2865 15 653 3105
Fragments arranged by size:
3105 2865 653 15
Bme1580I G_kGCm'C
Cuts at: 1034 1181 1534 1642 1682 1742 2102 2189 2576
Size: 147 353 108 40 60 360 87 387
Cuts at: 2576 2638 2748 2843 3092 3332 4263 4356 5480
Size: 62 110 95 249 240 931 93 1124
Cuts at: 5480 1034
Size: 2192
Fragments arranged by size:
2192 1124 931 387 360 353 249 240
147 110 108 95 93 87 62 60
40
BmgBI CAC'GTC
Cuts at: 261 1263 1665 261
Size: 1002 402 5234
Fragments arranged by size:
5234 1002 402
BmrI ACTGGGnnnn_n'
Cuts at: 2916 3850 3931 4266 6410 2916
Size: 934 81 335 2144 3144
Fragments arranged by size:
3144 2144 934 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5005 5005
Size: 6638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 694 1002 1768 1867 2215 3514 478
Size: 216 308 766 99 348 1299 3602
Fragments arranged by size:
3602 1299 766 348 308 216 99
Bpu10I CC'TnA_GC
Cuts at: 190 703 1774 2435 2566 2660 3355 3415 190
Size: 513 1071 661 131 94 695 60 3413
Fragments arranged by size:
3413 1071 695 661 513 131 94 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4693 5407 5705 3814
Size: 879 714 298 4747
Fragments arranged by size:
4747 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 616 1896 2805 6342 616
Size: 1280 909 3537 912
Fragments arranged by size:
3537 1280 912 909
BsaAI yAC'GTr
Cuts at: 3991 3991
Size: 6638
BsaBI GATnn'nnATC
Cuts at: 6 1465 4152 6189 6
Size: 1459 2687 2037 455
Fragments arranged by size:
2687 2037 1459 455
BsaHI Gr'CG_yC
Cuts at: 680 2729 3298 4069 4317 5019 6515 680
Size: 2049 569 771 248 702 1496 803
Fragments arranged by size:
2049 1496 803 771 702 569 248
BsaWI w'CCGG_w
Cuts at: 148 762 3827 4348 5026 5437 5584 5858 148
Size: 614 3065 521 678 411 147 274 928
Fragments arranged by size:
3065 928 678 614 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 577 1393 1954 186
Size: 391 816 561 4870
Fragments arranged by size:
4870 816 561 391
BseYI C'CCAG_C
Cuts at: 280 545 626 779 1173 1838 1922 2188 2204
Size: 265 81 153 394 665 84 266 16
Cuts at: 2204 3167 3453 4063 4821 5486 5894 280
Size: 963 286 610 758 665 408 1024
Fragments arranged by size:
1024 963 758 665 665 610 408 394
286 266 265 153 84 81 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1208 1278 3157 448
Size: 760 70 1879 3929
Fragments arranged by size:
3929 1879 760 70
BsiEI CG_ry'CG
Cuts at: 104 3313 4180 4226 5456 104
Size: 3209 867 46 1230 1286
Fragments arranged by size:
3209 1286 1230 867 46
BsiHKAI G_wGCw'C
Cuts at: 1123 1321 1534 1755 3023 3098 3361 3652 4430
Size: 198 213 221 1268 75 263 291 778
Cuts at: 4430 4620 5480 1123
Size: 190 860 2281
Fragments arranged by size:
2281 1268 860 778 291 263 221 213
198 190 75
BsmI GAATG_Cn'
Cuts at: 1139 1696 1139
Size: 557 6081
BsmBI CGTCTCn'nnnn_
Cuts at: 2717 2717
Size: 6638
Bsp1286I G_dGCh'C
Cuts at: 1034 1123 1181 1321 1476 1534 1642 1682 1742
Size: 89 58 140 155 58 108 40 60
Cuts at: 1742 1755 1922 1946 2081 2102 2189 2576 2638
Size: 13 167 24 135 21 87 387 62
Cuts at: 2638 2748 2843 3023 3092 3098 3332 3361 3652
Size: 110 95 180 69 6 234 29 291
Cuts at: 3652 4263 4356 4430 4620 4682 5480 1034
Size: 611 93 74 190 62 798 2192
Fragments arranged by size:
2192 798 611 387 291 234 190 180
167 155 140 135 110 108 95 93
89 87 74 69 62 62 60 58
58 40 29 24 21 13 6
BspHI T'CATG_A
Cuts at: 2261 3023 5070 2261
Size: 762 2047 3829
Fragments arranged by size:
3829 2047 762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1512 1771 2360 2981 3224 3688 3902 4204 4585
Size: 259 589 621 243 464 214 302 381
Cuts at: 4585 1512
Size: 3565
Fragments arranged by size:
3565 621 589 464 381 302 259 243
214
BsrBI CCG'CTC
Cuts at: 50 668 689 2439 3315 4930 4984 6468 6633
Size: 618 21 1750 876 1615 54 1484 165
Cuts at: 6633 50
Size: 55
Fragments arranged by size:
1750 1615 1484 876 618 165 55 54
21
BsrDI GCAATG_nn'
Cuts at: 1940 4550 1940
Size: 2610 4028
BsrFI r'CCGG_y
Cuts at: 148 762 986 1096 1199 1778 1857 3130 3308
Size: 614 224 110 103 579 79 1273 178
Cuts at: 3308 3433 3619 4636 4817 148
Size: 125 186 1017 181 1969
Fragments arranged by size:
1969 1273 1017 614 579 224 186 181
178 125 110 103 79
BsrGI T'GTAC_A
Cuts at: 917 2909 917
Size: 1992 4646
BssSI C'ACGA_G
Cuts at: 130 4909 5617 130
Size: 4779 708 1151
Fragments arranged by size:
4779 1151 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1574 4015 1574
Size: 2441 4197
BstBI TT'CG_AA
Cuts at: 4999 4999
Size: 6638
BstEII G'GTnAC_C
Cuts at: 1275 1485 2968 3126 3462 1275
Size: 210 1483 158 336 4451
Fragments arranged by size:
4451 1483 336 210 158
BstXI CCAn_nnnn'nTGG
Cuts at: 1063 2559 2625 2994 3012 1063
Size: 1496 66 369 18 4689
Fragments arranged by size:
4689 1496 369 66 18
BstYI r'GATC_y
Cuts at: 1 183 284 2235 2505 3663 4130 4153 4488
Size: 182 101 1951 270 1158 467 23 335
Cuts at: 4488 4734 5040 5052 5138 6190 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1951 1158 1052 467 449 335 306 270
246 182 101 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3628 3628
Size: 6638
BtgI C'CryG_G
Cuts at: 238 660 696 1490 1796 1808 2531 2843 3172
Size: 422 36 794 306 12 723 312 329
Cuts at: 3172 3236 238
Size: 64 3640
Fragments arranged by size:
3640 794 723 422 329 312 306 64
36 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 819 4588 4769 4932 819
Size: 3769 181 163 2525
Fragments arranged by size:
3769 2525 181 163
BtsI GCAGTG_nn'
Cuts at: 1159 1277 1585 1901 2173 3023 3881 1159
Size: 118 308 316 272 850 858 3916
Fragments arranged by size:
3916 858 850 316 308 272 118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2721 2756 2721
Size: 35 6603
DraI TTT'AAA
Cuts at: 3637 3637
Size: 6638
DraIII CAC_nnn'GTG
Cuts at: 264 264
Size: 6638
DrdI GACnn_nn'nnGTC
Cuts at: 4344 5688 4344
Size: 1344 5294
EaeI y'GGCC_r
Cuts at: 489 663 988 1094 1757 2020 2162 2822 2984
Size: 174 325 106 663 263 142 660 162
Cuts at: 2984 3064 3200 3310 3590 3604 4223 4397 4788
Size: 80 136 110 280 14 619 174 391
Cuts at: 4788 4815 6160 489
Size: 27 1345 967
Fragments arranged by size:
1345 967 663 660 619 391 325 280
263 174 174 162 142 136 110 106
80 27 14
EagI C'GGCC_G
Cuts at: 3310 4223 3310
Size: 913 5725
EarI CTCTTCn'nnn_
Cuts at: 304 418 598 998 1219 1847 1989 4661 4871
Size: 114 180 400 221 628 142 2672 210
Cuts at: 4871 304
Size: 2071
Fragments arranged by size:
2672 2071 628 400 221 210 180 142
114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3295 4846 5588 5734 6448 3295
Size: 1551 742 146 714 3485
Fragments arranged by size:
3485 1551 742 714 146
EcoICRI GAG'CTC
Cuts at: 1121 1319 3359 3650 1121
Size: 198 2040 291 4109
Fragments arranged by size:
4109 2040 291 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 694 813 1002 1171 1705 1768 1867 2215
Size: 216 119 189 169 534 63 99 348
Cuts at: 2215 2296 2683 2770 3514 4463 4895 5248 5910
Size: 81 387 87 744 949 432 353 662
Cuts at: 5910 478
Size: 1206
Fragments arranged by size:
1206 949 744 662 534 432 387 353
348 216 189 169 119 99 87 81
63
EcoNI CCTnn'n_nnAGG
Cuts at: 595 1648 2629 3397 595
Size: 1053 981 768 3836
Fragments arranged by size:
3836 1053 981 768
EcoO109I rG'GnC_Cy
Cuts at: 158 1177 1178 1223 1308 1738 1739 1842 1880
Size: 1019 1 45 85 430 1 103 38
Cuts at: 1880 2315 2622 2744 2745 3482 3777 158
Size: 435 307 122 1 737 295 3019
Fragments arranged by size:
3019 1019 737 435 430 307 295 122
103 85 45 38 1 1 1
EcoRI G'AATT_C
Cuts at: 3642 3642
Size: 6638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3582 4657 3582
Size: 1075 5563
FspI TGC'GCA
Cuts at: 1591 4419 1591
Size: 2828 3810
HaeII r_GCGC'y
Cuts at: 609 2732 2865 3046 3079 3301 3352 3415 3567
Size: 2123 133 181 33 222 51 63 152
Cuts at: 3567 4072 4320 5550 6321 609
Size: 505 248 1230 771 926
Fragments arranged by size:
2123 1230 926 771 505 248 222 181
152 133 63 51 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1247 1279 2533 2565 4480 4510 4512 4542 1247
Size: 32 1254 32 1915 30 2 30 3343
Fragments arranged by size:
3343 1915 1254 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3677 3677
Size: 6638
HindIII A'AGCT_T
Cuts at: 2695 2695
Size: 6638
KasI G'GCGC_C
Cuts at: 2728 3297 4068 4316 2728
Size: 569 771 248 5050
Fragments arranged by size:
5050 771 569 248
KpnI G_GTAC'C
Cuts at: 520 3658 520
Size: 3138 3500
MluI A'CGCG_T
Cuts at: 6071 6071
Size: 6638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 286 5397 5581 286
Size: 5111 184 1343
Fragments arranged by size:
5111 1343 184
MscI TGG'CCA
Cuts at: 1759 2022 2164 2824 2986 4399 1759
Size: 263 142 660 162 1413 3998
Fragments arranged by size:
3998 1413 660 263 162 142
MslI CAynn'nnrTG
Cuts at: 973 1144 1525 1795 2005 2488 3010 3325 4627
Size: 171 381 270 210 483 522 315 1302
Cuts at: 4627 4754 973
Size: 127 2857
Fragments arranged by size:
2857 1302 522 483 381 315 270 210
171 127
MspA1I CmG'CkG
Cuts at: 221 783 1617 1926 2137 2362 2570 3408 3576
Size: 562 834 309 211 225 208 838 168
Cuts at: 3576 3612 3749 4063 4423 5207 5452 221
Size: 36 137 314 360 784 245 1407
Fragments arranged by size:
1407 838 834 784 562 360 314 309
245 225 211 208 168 137 36
NaeI GCC'GGC
Cuts at: 988 1098 1780 1859 3310 3621 4819 988
Size: 110 682 79 1451 311 1198 2807
Fragments arranged by size:
2807 1451 1198 682 311 110 79
NarI GG'CG_CC
Cuts at: 2729 3298 4069 4317 2729
Size: 569 771 248 5050
Fragments arranged by size:
5050 771 569 248
NcoI C'CATG_G
Cuts at: 238 660 696 1808 2843 3172 238
Size: 422 36 1112 1035 329 3704
Fragments arranged by size:
3704 1112 1035 422 329 36
NgoMIV G'CCGG_C
Cuts at: 986 1096 1778 1857 3308 3619 4817 986
Size: 110 682 79 1451 311 1198 2807
Fragments arranged by size:
2807 1451 1198 682 311 110 79
NspI r_CATG'y
Cuts at: 2718 3691 4191 5794 2718
Size: 973 500 1603 3562
Fragments arranged by size:
3562 1603 973 500
PciI A'CATG_T
Cuts at: 5790 5790
Size: 6638
PflMI CCAn_nnn'nTGG
Cuts at: 786 1890 786
Size: 1104 5534
PfoI T'CCnGG_A
Cuts at: 653 710 1784 1844 653
Size: 57 1074 60 5447
Fragments arranged by size:
5447 1074 60 57
PmeI GTTT'AAAC
Cuts at: 3637 3637
Size: 6638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5084 5116 5084
Size: 32 6606
PpuMI rG'GwC_Cy
Cuts at: 158 1223 1842 1880 2315 2622 158
Size: 1065 619 38 435 307 4174
Fragments arranged by size:
4174 1065 619 435 307 38
PshAI GACnn'nnGTC
Cuts at: 1885 1885
Size: 6638
PspOMI G'GGCC_C
Cuts at: 1177 1738 2572 2744 1177
Size: 561 834 172 5071
Fragments arranged by size:
5071 834 561 172
PspXI vC'TCGA_Gb
Cuts at: 2814 2814
Size: 6638
PstI C_TGCA'G
Cuts at: 1616 1866 1931 1997 2126 2357 2978 3116 3282
Size: 250 65 66 129 231 621 138 166
Cuts at: 3282 3401 3685 4370 1616
Size: 119 284 685 3884
Fragments arranged by size:
3884 685 621 284 250 231 166 138
129 119 66 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6638
PvuII CAG'CTG
Cuts at: 783 1617 1926 2137 3576 4063 4423 783
Size: 834 309 211 1439 487 360 2998
Fragments arranged by size:
2998 1439 834 487 360 309 211
RsrII CG'GwC_CG
Cuts at: 4833 4833
Size: 6638
SacI G_AGCT'C
Cuts at: 1123 1321 3361 3652 1123
Size: 198 2040 291 4109
Fragments arranged by size:
4109 2040 291 198
SalI G'TCGA_C
Cuts at: 3675 3675
Size: 6638
SanDI GG'GwC_CC
Cuts at: 158 1223 1880 158
Size: 1065 657 4916
Fragments arranged by size:
4916 1065 657
SapI GCTCTTCn'nnn_
Cuts at: 304 418 1847 4661 4871 304
Size: 114 1429 2814 210 2071
Fragments arranged by size:
2814 2071 1429 210 114
SbfI CC_TGCA'GG
Cuts at: 3401 3685 3401
Size: 284 6354
ScaI AGT'ACT
Cuts at: 2704 6155 2704
Size: 3451 3187
SexAI A'CCwGG_T
Cuts at: 641 1481 2993 641
Size: 840 1512 4286
Fragments arranged by size:
4286 1512 840
SfcI C'TryA_G
Cuts at: 33 1416 1612 1862 1927 1993 2122 2353 2689
Size: 1383 196 250 65 66 129 231 336
Cuts at: 2689 2974 3112 3278 3397 3681 4366 5334 5525
Size: 285 138 166 119 284 685 968 191
Cuts at: 5525 33
Size: 1146
Fragments arranged by size:
1383 1146 968 685 336 285 284 250
231 196 191 166 138 129 119 66
65
SfoI GGC'GCC
Cuts at: 2730 3299 4070 4318 2730
Size: 569 771 248 5050
Fragments arranged by size:
5050 771 569 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6638
SgrAI Cr'CCGG_yG
Cuts at: 1199 1199
Size: 6638
SmaI CCC'GGG
Cuts at: 1243 2377 3660 1243
Size: 1134 1283 4221
Fragments arranged by size:
4221 1283 1134
SmlI C'TyrA_G
Cuts at: 2458 2814 3793 4708 5422 5684 2458
Size: 356 979 915 714 262 3412
Fragments arranged by size:
3412 979 915 714 356 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6638
SphI G_CATG'C
Cuts at: 3691 4191 3691
Size: 500 6138
SrfI GCCC'GGGC
Cuts at: 1243 1243
Size: 6638
StuI AGG'CCT
Cuts at: 826 2044 826
Size: 1218 5420
StyI C'CwwG_G
Cuts at: 238 660 696 1808 2618 2843 3172 3290 3772
Size: 422 36 1112 810 225 329 118 482
Cuts at: 3772 4126 238
Size: 354 2750
Fragments arranged by size:
2750 1112 810 482 422 354 329 225
118 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 999 1598 2443 2956 4221 4354 4813 5022 999
Size: 599 845 513 1265 133 459 209 2615
Fragments arranged by size:
2615 1265 845 599 513 459 209 133
TatI w'GTAC_w
Cuts at: 917 2702 2897 2909 6153 917
Size: 1785 195 12 3244 1402
Fragments arranged by size:
3244 1785 1402 195 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6155 6187 6155
Size: 32 6606
Tth111I GACn'n_nGTC
Cuts at: 679 955 2995 4435 679
Size: 276 2040 1440 2882
Fragments arranged by size:
2882 2040 1440 276
XbaI T'CTAG_A
Cuts at: 3669 3669
Size: 6638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 484 505 527 4133 484
Size: 21 22 3606 2989
Fragments arranged by size:
3606 2989 22 21
XhoI C'TCGA_G
Cuts at: 2458 2814 2458
Size: 356 6282
XmaI C'CCGG_G
Cuts at: 1241 2375 3658 1241
Size: 1134 1283 4221
Fragments arranged by size:
4221 1283 1134
XmnI GAAnn'nnTTC
Cuts at: 2599 3565 2599
Size: 966 5672
ZraI GAC'GTC
Cuts at: 681 681
Size: 6638
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BaeI BfrBI BmtI BsaXI
BsiWI BspEI BssHII BstZ17I ClaI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PsiI PsrI SacII SfiI SnaBI SspI
SwaI