Restriction map for pF1KB9491
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccI        AcyI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp718I     AspEI       AvaII       
AviII       AvrII       BanI        BbeI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BglI        
BlnI        BsaAI       BsaHI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MluI        
NarI        NcoI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII          60  CGCG                 CG^CG
AccIII       2384  TCCGGA               T^CCGGA
AclI         1109  AACGTT               AA^CGTT
AflIII       2250  ACATGT               A^C[GA][CT]GT
AlwNI        2104  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         3322  GGGCCC               GGGCC^C
AseI         3121  ATTAAT               AT^TAAT
AsnI         3121  ATTAAT               AT^TAAT
AspI          456  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI            9  CCCGGG               C^[CT]CG[GA]G
BalI         1656  TGGCCA               TGG^CCA
BamHI        3243  GGATCC               G^GATCC
BcgI          134  GCAAGAAAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         3133  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     3133  GCTAAGC              GC^T[ACGT]AGC
BsaBI        1576  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2384  TCCGGA               [AT]^CCGG[AT]
BseAI        2384  TCCGGA               T^CCGGA
BsgI          425  GTGCAG               GTGCAG(16/14)
BsoBI           9  CCCGGG               C^[CT]CG[GA]G
BspEI        2384  TCCGGA               T^CCGGA
BspLU11I     2250  ACATGT               A^CATGT
BspMI        1388  ACCTGC               ACCTGC(4/8)
BsrFI          69  GCCGGC               [GA]^CCGG[CT]
BstAPI       2145  GCAACAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          60  CGCG                 CG^CG
BstXI        2702  CCAAAGAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        3133  GCTAAGC              GC^T[ACGT]AGC
Cfr10I         69  GCCGGC               [GA]^CCGG[CT]
DraII        3321  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoNI        1566  CCTGGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     3321  AGGGCCC              [GA]G^G[ACGT]CC[CT]
FauI         2853  GCGGG                CCCGC(4/6)
HaeII        3386  AGCGCC               [GA]GCGC^[CT]
MamI         1576  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1656  TGGCCA               TGG^CCA
MroI         2384  TCCGGA               T^CCGGA
MscI         1656  TGGCCA               TGG^CCA
MvnI           60  CGCG                 CG^CG
NaeI           69  GCCGGC               GCC^GGC
NgoMIV         69  GCCGGC               G^CCGGC
NheI         1092  GCTAGC               G^CTAGC
PflFI         456  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        3121  ATTAAT               AT^TAAT
Psp1406I     1109  AACGTT               AA^CGTT
SacI         2300  GAGCTC               GAGCT^C
SapI         1297  GAAGAGC              GCTCTTC(1/4)
ScaI         1617  AGTACT               AGT^ACT
SmaI            9  CCCGGG               CCC^GGG
Tth111I       456  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           74  CCACCGCACAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI           9  CCCGGG               C^CCGGG
XmaI            9  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

Asp700I       232  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1741  GAAAGGCTTC          
BanII        2300  GAGCTC               G[GA]GC[CT]^C
             3322  GGGCCC              
BsaI         1786  GAGACC               GGTCTC(1/5)
             3008  GAGACC              
BsmFI         876  GGGAC                GGGAC(10/14)
              684  GTCCC               
BsmI         1160  GAATGC               GAATGC(1/-1)
             2328  GCATTC              
BsrDI        1999  GCAATG               GCAATG(2/0)
             3221  GCAATG              
DraI          171  TTTAAA               TTT^AAA
             2784  TTTAAA              
MaeII         780  ACGT                 A^CGT
             1110  ACGT                
NdeI         1471  CATATG               CA^TATG
             2595  CATATG              
NlaIV        3243  GGATCC               GG[ACGT]^[ACGT]CC
             3322  GGGCCC              
PflMI        1284  CCAAAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1665  CCAAGATATGG         
PstI         1703  CTGCAG               CTGCA^G
             2333  CTGCAG              
SspI          270  AATATT               AAT^ATT
             1364  AATATT              
Van91I       1284  CCAAAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1665  CCAAGATATGG         
XbaI          463  TCTAGA               T^CTAGA
             1317  TCTAGA              
XmnI          232  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1741  GAAAGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       BbsI        BbvI        BcnI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspHI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MfeI        MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        MwoI        NciI        NdeII       
NlaIII      NspI        PleI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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