Restriction map for pF1KB9491
 (Linear) (Six-base) MAPSORT of: KIBB9491.seq  Check: 4789  from: 1  to: 3420



KIBB9491.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AclI AA'CG_TT

Cuts at:      0    1110    3420 
   Size:       1110    2310
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    1112    1711    1732    2392    3420 
   Size:        961     151     599      21     660    1028

  Fragments arranged by size: 

                1028     961     660     599     151      21
 
AflIII A'CryG_T

Cuts at:      0    2250    3420 
   Size:       2250    1170
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     215     440    3420 
   Size:        215     225    2980

  Fragments arranged by size: 

                2980     225     215
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2109    3420 
   Size:       2109    1311
 
ApaI G_GGCC'C

Cuts at:      0    3326    3420 
   Size:       3326      94
 
ApoI r'AATT_y

Cuts at:      0      55     236     245     382     490     868    2713    2729 
   Size:         55     181       9     137     108     378    1845      16

Cuts at:   2729    3420 
   Size:        691

  Fragments arranged by size: 

                1845     691     378     181     137     108      55      16
                   9
 
AseI AT'TA_AT

Cuts at:      0    3122    3420 
   Size:       3122     298
 
AvaI C'yCGr_G

Cuts at:      0       9    3420 
   Size:          9    3411
 
BamHI G'GATC_C

Cuts at:      0    3243    3420 
   Size:       3243     177
 
BanII G_rGCy'C

Cuts at:      0    2304    3326    3420 
   Size:       2304    1022      94

  Fragments arranged by size: 

                2304    1022      94
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     569     946    1631    1717    2583    3420 
   Size:        569     377     685      86     866     837

  Fragments arranged by size: 

                 866     837     685     569     377      86
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     123     157    3420 
   Size:        123      34    3263

  Fragments arranged by size: 

                3263     123      34
 
BglII A'GATC_T

Cuts at:      0    1131    2073    2610    3420 
   Size:       1131     942     537     810

  Fragments arranged by size: 

                1131     942     810     537
 
BlpI GC'TnA_GC

Cuts at:      0    3134    3420 
   Size:       3134     286
 
Bme1580I G_kGCm'C

Cuts at:      0      95    2697    3326    3420 
   Size:         95    2602     629      94

  Fragments arranged by size: 

                2602     629      95      94
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2121    2154    3420 
   Size:       2121      33    1266

  Fragments arranged by size: 

                2121    1266      33
 
BmtI G_CTAG'C

Cuts at:      0    1096    3420 
   Size:       1096    2324
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1259    3362    3420 
   Size:       1259    2103      58

  Fragments arranged by size: 

                2103    1259      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1345    2131    3245    3347    3359    3420 
   Size:       1345     786    1114     102      12      61

  Fragments arranged by size: 

                1345    1114     786     102      61      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    2869    3084    3420 
   Size:        721    2148     215     336

  Fragments arranged by size: 

                2148     721     336     215
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1780    3002    3420 
   Size:       1780    1222     418

  Fragments arranged by size: 

                1780    1222     418
 
BsaBI GATnn'nnATC

Cuts at:      0    1580    3420 
   Size:       1580    1840
 
BsaWI w'CCGG_w

Cuts at:      0    2384    3420 
   Size:       2384    1036
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     667     697    3420 
   Size:        667      30    2723

  Fragments arranged by size: 

                2723     667      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     313     718    1423    2224    3420 
   Size:        313     405     705     801    1196

  Fragments arranged by size: 

                1196     801     705     405     313
 
BseYI C'CCAG_C

Cuts at:      0      88     146     434    3383    3420 
   Size:         88      58     288    2949      37

  Fragments arranged by size: 

                2949     288      88      58      37
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446    3420 
   Size:        446    2974
 
BsiHKAI G_wGCw'C

Cuts at:      0    2304    2594    3201    3420 
   Size:       2304     290     607     219

  Fragments arranged by size: 

                2304     607     290     219
 
BsmI GAATG_Cn'

Cuts at:      0    1166    2328    3420 
   Size:       1166    1162    1092

  Fragments arranged by size: 

                1166    1162    1092
 
Bsp1286I G_dGCh'C

Cuts at:      0      95    2304    2594    2697    3201    3326    3420 
   Size:         95    2209     290     103     504     125      94

  Fragments arranged by size: 

                2209     504     290     125     103      95      94
 
BspEI T'CCGG_A

Cuts at:      0    2384    3420 
   Size:       2384    1036
 
BspHI T'CATG_A

Cuts at:      0     225    2679    2816    3420 
   Size:        225    2454     137     604

  Fragments arranged by size: 

                2454     604     225     137
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1397    3420 
   Size:       1397    2023
 
BsrDI GCAATG_nn'

Cuts at:      0    2006    3228    3420 
   Size:       2006    1222     192

  Fragments arranged by size: 

                2006    1222     192
 
BsrFI r'CCGG_y

Cuts at:      0      69    3420 
   Size:         69    3351
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2151    3420 
   Size:       2151    1269
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2709    3420 
   Size:       2709     711
 
BstYI r'GATC_y

Cuts at:      0    1131    2073    2610    3243    3378    3420 
   Size:       1131     942     537     633     135      42

  Fragments arranged by size: 

                1131     942     633     537     135      42
 
DraI TTT'AAA

Cuts at:      0     173    2786    3420 
   Size:        173    2613     634

  Fragments arranged by size: 

                2613     634     173
 
EaeI y'GGCC_r

Cuts at:      0      50      71    1656    3420 
   Size:         50      21    1585    1764

  Fragments arranged by size: 

                1764    1585      50      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     470    1247    1292    1400    1565    1868    1955    1976 
   Size:        470     777      45     108     165     303      87      21

Cuts at:   1976    2270    2420    3420 
   Size:        294     150    1000

  Fragments arranged by size: 

                1000     777     470     303     294     165     150     108
                  87      45      21
 
EcoICRI GAG'CTC

Cuts at:      0    2302    3420 
   Size:       2302    1118
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    1112    1345    1711    1732    2131    2392    3245 
   Size:        961     151     233     366      21     399     261     853

Cuts at:   3245    3347    3359    3420 
   Size:        102      12      61

  Fragments arranged by size: 

                 961     853     399     366     261     233     151     102
                  61      21      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1570    3420 
   Size:       1570    1850
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3322    3420 
   Size:       3322      98
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     874    3013    3420 
   Size:        874    2139     407

  Fragments arranged by size: 

                2139     874     407
 
HaeII r_GCGC'y

Cuts at:      0    3390    3420 
   Size:       3390      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     667     699    1195    1225    1227    1257 
   Size:        181      32     454      32     496      30       2      30

Cuts at:   1257    1914    1946    1978    2010    2329    2361    2999    3031 
   Size:        657      32      32      32     319      32     638      32

Cuts at:   3031    3420 
   Size:        389

  Fragments arranged by size: 

                 657     638     496     454     389     319     181      32
                  32      32      32      32      32      32      30      30
                   2
 
HindIII A'AGCT_T

Cuts at:      0     389     973    1124    3356    3420 
   Size:        389     584     151    2232      64

  Fragments arranged by size: 

                2232     584     389     151      64
 
MfeI C'AATT_G

Cuts at:      0    1478    1853    2495    3420 
   Size:       1478     375     642     925

  Fragments arranged by size: 

                1478     925     642     375
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     468    2789    3420 
   Size:        468    2321     631

  Fragments arranged by size: 

                2321     631     468
 
MscI TGG'CCA

Cuts at:      0    1658    3420 
   Size:       1658    1762
 
MslI CAynn'nnrTG

Cuts at:      0     358    1496    2483    2678    3420 
   Size:        358    1138     987     195     742

  Fragments arranged by size: 

                1138     987     742     358     195
 
MspA1I CmG'CkG

Cuts at:      0      84    1099    2181    2491    2536    3054    3420 
   Size:         84    1015    1082     310      45     518     366

  Fragments arranged by size: 

                1082    1015     518     366     310      84      45
 
NaeI GCC'GGC

Cuts at:      0      71    3420 
   Size:         71    3349
 
NdeI CA'TA_TG

Cuts at:      0    1472    2596    3420 
   Size:       1472    1124     824

  Fragments arranged by size: 

                1472    1124     824
 
NgoMIV G'CCGG_C

Cuts at:      0      69    3420 
   Size:         69    3351
 
NheI G'CTAG_C

Cuts at:      0    1092    3420 
   Size:       1092    2328
 
NspI r_CATG'y

Cuts at:      0     417     442    2254    2488    3420 
   Size:        417      25    1812     234     932

  Fragments arranged by size: 

                1812     932     417     234      25
 
PciI A'CATG_T

Cuts at:      0    2250    3420 
   Size:       2250    1170
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1290    1671    3420 
   Size:       1290     381    1749

  Fragments arranged by size: 

                1749    1290     381
 
PsiI TTA'TAA

Cuts at:      0     395    3420 
   Size:        395    3025
 
PspOMI G'GGCC_C

Cuts at:      0    3322    3420 
   Size:       3322      98
 
PstI C_TGCA'G

Cuts at:      0    1707    2337    3420 
   Size:       1707     630    1083

  Fragments arranged by size: 

                1707    1083     630
 
PvuII CAG'CTG

Cuts at:      0      84    1099    2491    2536    3054    3420 
   Size:         84    1015    1392      45     518     366

  Fragments arranged by size: 

                1392    1015     518     366      84      45
 
SacI G_AGCT'C

Cuts at:      0    2304    3420 
   Size:       2304    1116
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1292    3420 
   Size:       1292    2128
 
ScaI AGT'ACT

Cuts at:      0    1619    3420 
   Size:       1619    1801
 
SfcI C'TryA_G

Cuts at:      0     105     829     902    1703    2333    3420 
   Size:        105     724      73     801     630    1087

  Fragments arranged by size: 

                1087     801     724     630     105      73
 
SmaI CCC'GGG

Cuts at:      0      11    3420 
   Size:         11    3409
 
SmlI C'TyrA_G

Cuts at:      0     700    2884    3099    3420 
   Size:        700    2184     215     321

  Fragments arranged by size: 

                2184     700     321     215
 
SspI AAT'ATT

Cuts at:      0     272    1366    3420 
   Size:        272    1094    2054

  Fragments arranged by size: 

                2054    1094     272
 
StyI C'CwwG_G

Cuts at:      0     155    1040    1790    3420 
   Size:        155     885     750    1630

  Fragments arranged by size: 

                1630     885     750     155
 
TatI w'GTAC_w

Cuts at:      0     528    1617    3420 
   Size:        528    1089    1803

  Fragments arranged by size: 

                1803    1089     528
 
Tth111I GACn'n_nGTC

Cuts at:      0     459    3420 
   Size:        459    2961
 
XbaI T'CTAG_A

Cuts at:      0     463    1317    3420 
   Size:        463     854    2103

  Fragments arranged by size: 

                2103     854     463
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      81    3420 
   Size:         81    3339
 
XmaI C'CCGG_G

Cuts at:      0       9    3420 
   Size:          9    3411
 
XmnI GAAnn'nnTTC

Cuts at:      0     236    1745    3420 
   Size:        236    1509    1675

  Fragments arranged by size: 

                1675    1509     236
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AlfI    AlwNI     ApaI     ApoI     AseI
     AvaI    BamHI    BanII     BbsI     BcgI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrDI    BsrFI   BstAPI    BstXI    BstYI     DraI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NdeI   NgoMIV     NheI     NspI     PciI    PflMI     PsiI   PspOMI
     PstI    PvuII     SacI     SapI     ScaI     SfcI     SmaI     SmlI
     SspI     StyI     TatI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI     BanI
     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BmgBI   Bpu10I
    BsaAI    BsaHI    BsiEI    BsiWI    BsmBI    BsrBI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     NarI     NcoI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TstI     XhoI     ZraI
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