Restriction map for pF1KB9484
##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AvrII       BamHI       BfrI        BglII       BlnI        
BsaBI       BsiWI       BsmBI       BspDI       BspHI       
BspLU11I    Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        Eco47III    EcoRV       EcoT22I     
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MseI        MunI        NdeI        NheI        
NsiI        NspV        PacI        PinAI       PmeI        
PshBI       Psp1406I    RcaI        RsrII       SacI        
SalI        ScaI        SfuI        SgrAI       SpeI        
SspI        SwaI        Tru9I       XbaI        


###################################
# ONE CUT ENZYMES (   14 enzymes) #
###################################

AviII        5690  TGCGCA               TGC^GCA
BbvCI        1781  CCTCAGC              CCTCAGC(-5/-2)
BciVI        5592  GGATAC               GTATCC(6/5)
BclI          278  TGATCA               T^GATCA
BfaI         4276  CTAG                 C^TAG
DrdI         1075  GACGACGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI          278  TGATCA               T^GATCA
FseI            3  GGCCGGCC             GGCCGG^CC
FspI         5690  TGCGCA               TGC^GCA
MaeI         4276  CTAG                 C^TAG
NruI         3064  TCGCGA               TCG^CGA
PvuI         1114  CGATCG               CGAT^CG
SfiI         5014  GGCCCCAGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         1733  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcsI         3534  GAATTC               [GA]^AATT[CT]
             4195  GAATTC              
Alw44I       1744  GTGCAC               G^TGCAC
             3616  GTGCAC              
ApaLI        1744  GTGCAC               G^TGCAC
             3616  GTGCAC              
ApoI         3534  GAATTC               [GA]^AATT[CT]
             4195  GAATTC              
Asp700I       921  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3549  GAAGGATTTC          
BlpI          708  GCTCAGC              GC^T[ACGT]AGC
             2750  GCTCAGC             
Bpu1102I      708  GCTCAGC              GC^T[ACGT]AGC
             2750  GCTCAGC             
Bsp1407I     2530  TGTACA               T^GTACA
             4229  TGTACA              
BsrGI        2530  TGTACA               T^GTACA
             4229  TGTACA              
BstEII       2166  GGTCACC              G^GT[ACGT]ACC
             5443  GGTCACC             
BstPI        2166  GGTCACC              G^GT[ACGT]ACC
             5443  GGTCACC             
CelII         708  GCTCAGC              GC^T[ACGT]AGC
             2750  GCTCAGC             
DraIII       2178  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3099  CACCGTGTG           
EcoO65I      2166  GGTCACC              G^GT[ACGT]ACC
             5443  GGTCACC             
EcoRI        3534  GAATTC               G^AATTC
             4195  GAATTC              
HincII       4573  GTCAAC               GT[CT]^[GA]AC
             5839  GTCAAC              
HindII       4573  GTCAAC               GT[CT]^[GA]AC
             5839  GTCAAC              
KspI          714  CCGCGG               CCGC^GG
             2663  CCGCGG              
NotI           30  GCGGCCGC             GC^GGCCGC
             3714  GCGGCCGC            
NspI           88  ACATGC               [GA]CATG^[CT]
             1733  GCATGC              
PaeR7I       1482  CTCGAG               C^TCGAG
             3253  CTCGAG              
SacII         714  CCGCGG               CCGC^GG
             2663  CCGCGG              
SapI          594  GAAGAGC              GCTCTTC(1/4)
             4764  GAAGAGC             
SbfI         1470  CCTGCAGG             CCTGCA^GG
             5895  CCTGCAGG            
SexAI         233  ACCTGGT              A^CC[AT]GGT
              314  ACCAGGT             
SmlI         1482  CTCGAG               C^T[CT][GA]AG
             3253  CTCGAG              
SnaBI        2227  TACGTA               TAC^GTA
             5290  TACGTA              
Sse8387I     1470  CCTGCAGG             CCTGCA^GG
             5895  CCTGCAGG            
SspBI        2530  TGTACA               T^GTACA
             4229  TGTACA              
StuI         4947  AGGCCT               AGG^CCT
             6020  AGGCCT              
TfiI         3175  GAATC                G^A[AT]TC
             5537  GATTC               
Tsp509I      3535  AATT                 ^AATT
             4196  AATT                
XhoI         1482  CTCGAG               C^TCGAG
             3253  CTCGAG              
XmnI          921  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3549  GAAGGATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  151 enzymes) #
#########################################

AatII       AccII       AccIII      AciI        AcyI        
AfaI        AflIII      AhdI        AluI        AlwI        
AlwNI       AocI        ApaI        AspEI       AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvI        
BcgI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspEI       BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssHII      
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       PflFI       PflMI       PleI        
PmaCI       PmlI        PpuMI       PshAI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmaI        StyI        
TaqI        TseI        Tsp45I      TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com