Restriction map for pF1KB9483
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AcyI        AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BspHI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       BstEII      
BstPI       BstXI       BstYI       CelII       Cfr10I      
CpoI        DraIII      EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       FseI        
HaeII       Hin1I       KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MunI        
NaeI        NarI        NciI        NgoMIV      NheI        
NotI        NruI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PstI        PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       Sse8387I    Tth111I     XbaI        XcmI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccI         2553  GTATAC               GT^[AC][GT]AC
AccII          91  CGCG                 CG^CG
AccIII         15  TCCGGA               T^CCGGA
AclI          860  AACGTT               AA^CGTT
AcsI         1953  AAATTT               [GA]^AATT[CT]
AhdI         1714  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1465  GATCC                GGATC(4/5)
AocI         1316  CCTTAGG              CC^T[ACGT]AGG
ApaI         2335  GGGCCC               GGGCC^C
ApoI         1953  AAATTT               [GA]^AATT[CT]
AspEI        1714  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          482  CTCGAG               C^[CT]CG[GA]G
AvaII         582  GGACC                G^G[AT]CC
AviII         627  TGCGCA               TGC^GCA
BalI          806  TGGCCA               TGG^CCA
BanI         1093  GGCACC               G^G[CT][GA]CC
BbsI          104  GTCTTC               GAAGAC(2/6)
BcgI         2184  GCAAGCCTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1572  GGATAC               GTATCC(6/5)
BclI          795  TGATCA               T^GATCA
BpuAI         104  GTCTTC               GAAGAC(2/6)
BsaAI        2330  TACGTG               [CT]AC^GT[GA]
BseAI          15  TCCGGA               T^CCGGA
BsoBI         482  CTCGAG               C^[CT]CG[GA]G
BspDI        2315  ATCGAT               AT^CGAT
BspEI          15  TCCGGA               T^CCGGA
BspMI        1129  ACCTGC               ACCTGC(4/8)
Bst1107I     2553  GTATAC               GTA^TAC
BstBI          61  TTCGAA               TT^CGAA
BstUI          91  CGCG                 CG^CG
BstZ17I      2553  GTATAC               GTA^TAC
Bsu36I       1316  CCTTAGG              CC^T[ACGT]AGG
ClaI         2315  ATCGAT               AT^CGAT
DrdI          196  GACAAAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          806  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1714  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1316  CCTTAGG              CC^T[ACGT]AGG
EcoT22I      2138  ATGCAT               ATGCA^T
FbaI          795  TGATCA               T^GATCA
FspI          627  TGCGCA               TGC^GCA
HgaI         1766  GCGTC                GACGC(5/10)
HincII        985  GTTAAC               GT[CT]^[GA]AC
HindII        985  GTTAAC               GT[CT]^[GA]AC
HpaI          985  GTTAAC               GTT^AAC
MluNI         806  TGGCCA               TGG^CCA
MroI           15  TCCGGA               T^CCGGA
MscI          806  TGGCCA               TGG^CCA
MslI         1378  CATTTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           91  CGCG                 CG^CG
NdeI         2248  CATATG               CA^TATG
NsiI         2138  ATGCAT               ATGCA^T
NspV           61  TTCGAA               TT^CGAA
PaeR7I        482  CTCGAG               C^TCGAG
PflMI         847  CCATCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I      860  AACGTT               AA^CGTT
SfcI         2217  CTATAG               C^T[GA][CT]AG
SfuI           61  TTCGAA               TT^CGAA
SmlI          482  CTCGAG               C^T[CT][GA]AG
SpeI         1233  ACTAGT               A^CTAGT
SphI         2163  GCATGC               GCATG^C
SwaI          632  ATTTAAAT             ATTT^AAAT
Van91I        847  CCATCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          482  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI        1414  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1504  CAGGAACTG           
Asp700I      1285  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2022  GAACATTTTC          
BpmI         1655  CTGGAG               CTGGAG(16/14)
             2425  CTGGAG              
BsaI         1262  GAGACC               GGTCTC(1/5)
              451  GGTCTC              
BsaWI          15  TCCGGA               [AT]^CCGG[AT]
             2530  ACCGGA              
BsgI         1131  CTGCAC               GTGCAG(16/14)
             1496  CTGCAC              
Bsp1407I     1188  TGTACA               T^GTACA
             2292  TGTACA              
BsrGI        1188  TGTACA               T^GTACA
             2292  TGTACA              
CfoI          628  GCGC                 GCG^C
             2167  GCGC                
EcoRV        1159  GATATC               GAT^ATC
             1638  GATATC              
FauI           89  CCCGC                CCCGC(4/6)
              352  GCGGG               
HapII          16  CCGG                 C^CGG
             2531  CCGG                
HhaI          628  GCGC                 GCG^C
             2167  GCGC                
HinP1I        628  GCGC                 G^CGC
             2167  GCGC                
HpaII          16  CCGG                 C^CGG
             2531  CCGG                
MspI           16  CCGG                 C^CGG
             2531  CCGG                
NcoI          809  CCATGG               C^CATGG
              974  CCATGG              
NspI         2163  GCATGC               [GA]CATG^[CT]
             2280  ACATGC              
PleI          148  GACTC                GAGTC(4/5)
             1720  GAGTC               
SacI          440  GAGCTC               GAGCT^C
             2428  GAGCTC              
SspBI        1188  TGTACA               T^GTACA
             2292  TGTACA              
XmnI         1285  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2022  GAACATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BanII       
BbvI        BfaI        BmyI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SspI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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