Restriction map for pF1KB9483
 (Linear) (Six-base) MAPSORT of: KIBB9483.seq  Check: 6407  from: 1  to: 2571



KIBB9483.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AccI GT'mk_AC

Cuts at:      0    2554    2571 
   Size:       2554      17
 
AclI AA'CG_TT

Cuts at:      0     861    2571 
   Size:        861    1710
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     152     173    1067    1271    1304    1393    1531 
   Size:         91      61      21     894     204      33      89     138

Cuts at:   1531    2571 
   Size:       1040

  Fragments arranged by size: 

                1040     894     204     138      91      89      61      33
                  21
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1719    2571 
   Size:       1719     852
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     365    2571 
   Size:        365    2206
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1419    1509    2571 
   Size:       1419      90    1062

  Fragments arranged by size: 

                1419    1062      90
 
ApaI G_GGCC'C

Cuts at:      0    2339    2571 
   Size:       2339     232
 
ApoI r'AATT_y

Cuts at:      0    1953    2571 
   Size:       1953     618
 
AvaI C'yCGr_G

Cuts at:      0     482    2571 
   Size:        482    2089
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2310    2343    2571 
   Size:       2310      33     228

  Fragments arranged by size: 

                2310     228      33
 
BanI G'GyrC_C

Cuts at:      0    1093    2571 
   Size:       1093    1478
 
BanII G_rGCy'C

Cuts at:      0     444    1626    2339    2432    2571 
   Size:        444    1182     713      93     139

  Fragments arranged by size: 

                1182     713     444     139      93
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      97    2571 
   Size:         97    2474
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2173    2207    2571 
   Size:       2173      34     364

  Fragments arranged by size: 

                2173     364      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1566    2571 
   Size:       1566    1005
 
BclI T'GATC_A

Cuts at:      0     795    2571 
   Size:        795    1776
 
BfrBI ATG'CAT

Cuts at:      0    2140    2571 
   Size:       2140     431
 
Bme1580I G_kGCm'C

Cuts at:      0    2339    2571 
   Size:       2339     232
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     635    2571 
   Size:        635    1936
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     573    2300    2571 
   Size:        573    1727     271

  Fragments arranged by size: 

                1727     573     271
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1676    2446    2571 
   Size:       1676     770     125

  Fragments arranged by size: 

                1676     770     125
 
Bpu10I CC'TnA_GC

Cuts at:      0     338    2571 
   Size:        338    2233
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     457    1256    2571 
   Size:        457     799    1315

  Fragments arranged by size: 

                1315     799     457
 
BsaAI yAC'GTr

Cuts at:      0    2332    2571 
   Size:       2332     239
 
BsaWI w'CCGG_w

Cuts at:      0      15    2530    2571 
   Size:         15    2515      41

  Fragments arranged by size: 

                2515      41      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     304     322     325     328     331     334     337 
   Size:         70     234      18       3       3       3       3       3

Cuts at:    337     500     527    2571 
   Size:        163      27    2044

  Fragments arranged by size: 

                2044     234     163      70      27      18       3       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1419    2012    2571 
   Size:       1419     593     559

  Fragments arranged by size: 

                1419     593     559
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1116    1481    2571 
   Size:       1116     365    1090

  Fragments arranged by size: 

                1116    1090     365
 
BsiHKAI G_wGCw'C

Cuts at:      0     444    1760    2432    2571 
   Size:        444    1316     672     139

  Fragments arranged by size: 

                1316     672     444     139
 
Bsp1286I G_dGCh'C

Cuts at:      0     444    1626    1760    2339    2432    2571 
   Size:        444    1182     134     579      93     139

  Fragments arranged by size: 

                1182     579     444     139     134      93
 
BspEI T'CCGG_A

Cuts at:      0      15    2571 
   Size:         15    2556
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1138    2571 
   Size:       1138    1433
 
BsrDI GCAATG_nn'

Cuts at:      0     604    1619    1716    2571 
   Size:        604    1015      97     855

  Fragments arranged by size: 

                1015     855     604      97
 
BsrGI T'GTAC_A

Cuts at:      0    1188    2292    2571 
   Size:       1188    1104     279

  Fragments arranged by size: 

                1188    1104     279
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1486    1495    1750    2571 
   Size:       1486       9     255     821

  Fragments arranged by size: 

                1486     821     255       9
 
BstBI TT'CG_AA

Cuts at:      0      62    2571 
   Size:         62    2509
 
BstZ17I GTA'TAC

Cuts at:      0    2555    2571 
   Size:       2555      16
 
Bsu36I CC'TnA_GG

Cuts at:      0    1317    2571 
   Size:       1317    1254
 
BtgI C'CryG_G

Cuts at:      0     809     974    2367    2571 
   Size:        809     165    1393     204

  Fragments arranged by size: 

                1393     809     204     165
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     371    2053    2571 
   Size:        371    1682     518

  Fragments arranged by size: 

                1682     518     371
 
BtsI GCAGTG_nn'

Cuts at:      0     347    2526    2571 
   Size:        347    2179      45

  Fragments arranged by size: 

                2179     347      45
 
ClaI AT'CG_AT

Cuts at:      0    2316    2571 
   Size:       2316     255
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1361    1396    2571 
   Size:       1361      35    1175

  Fragments arranged by size: 

                1361    1175      35
 
DraI TTT'AAA

Cuts at:      0     635     833    1310    1604    2571 
   Size:        635     198     477     294     967

  Fragments arranged by size: 

                 967     635     477     294     198
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     202    2571 
   Size:        202    2369
 
EaeI y'GGCC_r

Cuts at:      0     806    2571 
   Size:        806    1765
 
EarI CTCTTCn'nnn_

Cuts at:      0      71     290     299     491    1097    2475    2571 
   Size:         71     219       9     192     606    1378      96

  Fragments arranged by size: 

                1378     606     219     192      96      71       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      84    2571 
   Size:         84    2487
 
EcoICRI GAG'CTC

Cuts at:      0     442    2430    2571 
   Size:        442    1988     141

  Fragments arranged by size: 

                1988     442     141
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     152     173    1067    1271    1304    1393    1531 
   Size:         91      61      21     894     204      33      89     138

Cuts at:   1531    1676    2446    2571 
   Size:        145     770     125

  Fragments arranged by size: 

                 894     770     204     145     138     125      91      89
                  61      33      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     963    2336    2421    2571 
   Size:        963    1373      85     150

  Fragments arranged by size: 

                1373     963     150      85
 
EcoRV GAT'ATC

Cuts at:      0    1161    1640    2571 
   Size:       1161     479     931

  Fragments arranged by size: 

                1161     931     479
 
FspI TGC'GCA

Cuts at:      0     629    2571 
   Size:        629    1942
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     780     812    1684    1705    1716    1737    2571 
   Size:        780      32     872      21      11      21     834

  Fragments arranged by size: 

                 872     834     780      32      21      21      11
 
HincII GTy'rAC

Cuts at:      0     987    2571 
   Size:        987    1584
 
HindIII A'AGCT_T

Cuts at:      0     944    1031    1286    2571 
   Size:        944      87     255    1285

  Fragments arranged by size: 

                1285     944     255      87
 
HpaI GTT'AAC

Cuts at:      0     987    2571 
   Size:        987    1584
 
MscI TGG'CCA

Cuts at:      0     808    2571 
   Size:        808    1763
 
MslI CAynn'nnrTG

Cuts at:      0    1382    2571 
   Size:       1382    1189
 
MspA1I CmG'CkG

Cuts at:      0     130     873     997    1419    2571 
   Size:        130     743     124     422    1152

  Fragments arranged by size: 

                1152     743     422     130     124
 
NcoI C'CATG_G

Cuts at:      0     809     974    2571 
   Size:        809     165    1597

  Fragments arranged by size: 

                1597     809     165
 
NdeI CA'TA_TG

Cuts at:      0    2249    2571 
   Size:       2249     322
 
NsiI A_TGCA'T

Cuts at:      0    2142    2571 
   Size:       2142     429
 
NspI r_CATG'y

Cuts at:      0    2167    2284    2571 
   Size:       2167     117     287

  Fragments arranged by size: 

                2167     287     117
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     853    2571 
   Size:        853    1718
 
PspOMI G'GGCC_C

Cuts at:      0    2335    2571 
   Size:       2335     236
 
PspXI vC'TCGA_Gb

Cuts at:      0     482    2571 
   Size:        482    2089
 
PvuII CAG'CTG

Cuts at:      0     130     873     997    1419    2571 
   Size:        130     743     124     422    1152

  Fragments arranged by size: 

                1152     743     422     130     124
 
SacI G_AGCT'C

Cuts at:      0     444    2432    2571 
   Size:        444    1988     139

  Fragments arranged by size: 

                1988     444     139
 
SfcI C'TryA_G

Cuts at:      0    2217    2571 
   Size:       2217     354
 
SmlI C'TyrA_G

Cuts at:      0     482    2571 
   Size:        482    2089
 
SpeI A'CTAG_T

Cuts at:      0    1233    2571 
   Size:       1233    1338
 
SphI G_CATG'C

Cuts at:      0    2167    2571 
   Size:       2167     404
 
SspI AAT'ATT

Cuts at:      0     696    1748    1876    2571 
   Size:        696    1052     128     695

  Fragments arranged by size: 

                1052     696     695     128
 
StuI AGG'CCT

Cuts at:      0     420    1315    2234    2571 
   Size:        420     895     919     337

  Fragments arranged by size: 

                 919     895     420     337
 
StyI C'CwwG_G

Cuts at:      0     585     809     974    2571 
   Size:        585     224     165    1597

  Fragments arranged by size: 

                1597     585     224     165
 
SwaI ATTT'AAAT

Cuts at:      0     635    2571 
   Size:        635    1936
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     448     781    2571 
   Size:        448     333    1790

  Fragments arranged by size: 

                1790     448     333
 
TatI w'GTAC_w

Cuts at:      0    1188    2292    2571 
   Size:       1188    1104     279

  Fragments arranged by size: 

                1188    1104     279
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1771    1803    2165    2197    2571 
   Size:       1771      32     362      32     374

  Fragments arranged by size: 

                1771     374     362      32      32
 
XhoI C'TCGA_G

Cuts at:      0     482    2571 
   Size:        482    2089
 
XmnI GAAnn'nnTTC

Cuts at:      0    1289    2026    2571 
   Size:       1289     737     545

  Fragments arranged by size: 

                1289     737     545
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AhdI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BaeI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI
    BsrDI    BsrGI   BstAPI    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRV     FspI    Hin4I   HincII  HindIII
     HpaI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
    PflMI   PspOMI    PspXI    PvuII     SacI     SfcI     SmlI     SpeI
     SphI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AleI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI     BbeI
    BbvCI     BglI    BglII     BlpI    BmgBI     BmtI    BpuEI    BsaBI
    BsaHI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI
    BsrFI   BssHII    BssSI   BstEII    BstXI    BstYI   DraIII     EagI
    EcoNI    EcoRI     FalI     FseI    FspAI    HaeII     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SrfI  Tth111I     XbaI     XcmI
     XmaI     ZraI
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