Restriction map for pF1KB9475
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       DrdI        DsaI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcsI         1024  AAATTC               [GA]^AATT[CT]
AflIII        880  ACACGT               A^C[GA][CT]GT
AhdI          790  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         1024  AAATTC               [GA]^AATT[CT]
AspEI         790  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         769  GAGCAC               G[AT]GC[AT]^C
BalI           28  TGGCCA               TGG^CCA
BanI          822  GGTGCC               G^G[CT][GA]CC
BanII          78  GGGCTC               G[GA]GC[CT]^C
BciVI         397  GTATCC               GTATCC(6/5)
BfaI          553  CTAG                 C^TAG
BglII          15  AGATCT               A^GATCT
BsaI          229  GAGACC               GGTCTC(1/5)
BsiHKAI       769  GAGCAC               G[AT]GC[AT]^C
BsiYI         826  CCTTCACCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          826  CCTTCACCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI         138  ACCTGC               ACCTGC(4/8)
BsrDI         461  GCAATG               GCAATG(2/0)
BstYI          15  AGATCT               [GA]^GATC[CT]
DpnII          16  GATC                 ^GATC
DraI          955  TTTAAA               TTT^AAA
EaeI           28  TGGCCA               [CT]^GGCC[GA]
Eam1105I      790  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         826  CCTTCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         292  GATATC               GAT^ATC
EcoT14I        96  CCAAGG               C^C[AT][AT]GG
FauI          405  CCCGC                CCCGC(4/6)
HindIII       177  AAGCTT               A^AGCTT
HphI          829  TCACC                GGTGA(8/7)
MaeI          553  CTAG                 C^TAG
MaeII         882  ACGT                 A^CGT
MboI           16  GATC                 ^GATC
MflI           15  AGATCT               [GA]^GATC[CT]
MluNI          28  TGGCCA               TGG^CCA
MscI           28  TGGCCA               TGG^CCA
MspA1I        536  CAGCTG               C[AC]G^C[GT]G
MwoI          696  GCAGCAGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII          16  GATC                 ^GATC
PshAI         628  GACTCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         536  CAGCTG               CAG^CTG
SapI          665  GAAGAGC              GCTCTTC(1/4)
Sau3AI         16  GATC                 ^GATC
SexAI         392  ACCAGGT              A^CC[AT]GGT
StyI           96  CCAAGG               C^C[AT][AT]GG
TaqI           20  TCGA                 T^CGA
TfiI          898  GAATC                G^A[AT]TC
Tsp509I      1025  AATT                 ^AATT
TthHB8I        20  TCGA                 T^CGA
XbaI          552  TCTAGA               T^CTAGA
XhoII          15  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI            7  CCGC                 CCGC(-3/-1)
              406  CCGC                
AfaI          136  GTAC                 GT^AC
              456  GTAC                
AlwNI          70  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              700  CAGAGGCTG           
BmyI           78  GGGCTC               G[AGT]GC[ACT]^C
              769  GAGCAC              
BpmI          278  CTCCAG               CTGGAG(16/14)
              637  CTCCAG              
Bsp1286I       78  GGGCTC               G[AGT]GC[ACT]^C
              769  GAGCAC              
DraIII        146  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              881  CACGTCGTG           
MseI          892  TTAA                 T^TAA
              956  TTAA                
NspI          447  ACATGC               [GA]CATG^[CT]
              670  GCATGT              
PstI          203  CTGCAG               CTGCA^G
              852  CTGCAG              
RsaI          136  GTAC                 GT^AC
              456  GTAC                
SfcI          203  CTGCAG               C^T[GA][CT]AG
              852  CTGCAG              
Tru9I         892  TTAA                 T^TAA
              956  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AluI        AvaII       BbsI        BbvI        BpuAI       
BsaJI       BsmAI       BsmFI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       ItaI        Ksp632I     MaeIII      MboII       
MnlI        MvaI        NlaIII      NlaIV       PleI        
Sau96I      ScrFI       SfaNI       TseI        Tsp45I      
TspRI       
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