Restriction map for pF1KB9475
 (Linear) (Six-base) MAPSORT of: KIBB9475.seq  Check: 9685  from: 1  to: 1029



KIBB9475.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     140     674     727     764     785     801    1029 
   Size:        106      34     534      53      37      21      16     228

  Fragments arranged by size: 

                 534     228     106      53      37      34      21      16
            
 
AflIII A'CryG_T

Cuts at:      0     880    1029 
   Size:        880     149
 
AhdI GACnn_n'nnGTC

Cuts at:      0     795    1029 
   Size:        795     234
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     880    1029 
   Size:        880     149
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     705    1029 
   Size:         75     630     324

  Fragments arranged by size: 

                 630     324      75
 
ApoI r'AATT_y

Cuts at:      0    1024    1029 
   Size:       1024       5
 
BanI G'GyrC_C

Cuts at:      0     822    1029 
   Size:        822     207
 
BanII G_rGCy'C

Cuts at:      0      82    1029 
   Size:         82     947
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      34      83     791    1029 
   Size:         34      49     708     238

  Fragments arranged by size: 

                 708     238      49      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     408    1029 
   Size:        408     621
 
BglII A'GATC_T

Cuts at:      0      15    1029 
   Size:         15    1014
 
BmgBI CAC'GTC

Cuts at:      0     883    1029 
   Size:        883     146
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      41    1029 
   Size:         41     988
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     147    1029 
   Size:        147     882
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     622    1029 
   Size:        263     359     407

  Fragments arranged by size: 

                 407     359     263
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     223    1029 
   Size:        223     806
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     676     706    1029 
   Size:        676      30     323

  Fragments arranged by size: 

                 676     323      30
 
BseYI C'CCAG_C

Cuts at:      0     233    1029 
   Size:        233     796
 
BsiHKAI G_wGCw'C

Cuts at:      0     773    1029 
   Size:        773     256
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     773    1029 
   Size:         82     691     256

  Fragments arranged by size: 

                 691     256      82
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     147    1029 
   Size:        147     882
 
BsrDI GCAATG_nn'

Cuts at:      0     468    1029 
   Size:        468     561
 
BstYI r'GATC_y

Cuts at:      0      15    1029 
   Size:         15    1014
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     420     455    1029 
   Size:        420      35     574

  Fragments arranged by size: 

                 574     420      35
 
DraI TTT'AAA

Cuts at:      0     957    1029 
   Size:        957      72
 
DraIII CAC_nnn'GTG

Cuts at:      0     151     886    1029 
   Size:        151     735     143

  Fragments arranged by size: 

                 735     151     143
 
EaeI y'GGCC_r

Cuts at:      0      28    1029 
   Size:         28    1001
 
EarI CTCTTCn'nnn_

Cuts at:      0     116     660     836    1029 
   Size:        116     544     176     193

  Fragments arranged by size: 

                 544     193     176     116
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     140     263     622     674     727     764     785 
   Size:        106      34     123     359      52      53      37      21

Cuts at:    785     801    1029 
   Size:         16     228

  Fragments arranged by size: 

                 359     228     123     106      53      52      37      34
                  21      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     830    1029 
   Size:        830     199
 
EcoRV GAT'ATC

Cuts at:      0     294    1029 
   Size:        294     735
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     195    1029 
   Size:        195     834
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     781     813    1029 
   Size:        781      32     216

  Fragments arranged by size: 

                 781     216      32
 
HindIII A'AGCT_T

Cuts at:      0     177    1029 
   Size:        177     852
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     967    1029 
   Size:        967      62
 
MscI TGG'CCA

Cuts at:      0      30    1029 
   Size:         30     999
 
MspA1I CmG'CkG

Cuts at:      0     538    1029 
   Size:        538     491
 
NspI r_CATG'y

Cuts at:      0     451     674    1029 
   Size:        451     223     355

  Fragments arranged by size: 

                 451     355     223
 
PshAI GACnn'nnGTC

Cuts at:      0     632    1029 
   Size:        632     397
 
PstI C_TGCA'G

Cuts at:      0     207     856    1029 
   Size:        207     649     173

  Fragments arranged by size: 

                 649     207     173
 
PvuII CAG'CTG

Cuts at:      0     538    1029 
   Size:        538     491
 
SapI GCTCTTCn'nnn_

Cuts at:      0     660    1029 
   Size:        660     369
 
SexAI A'CCwGG_T

Cuts at:      0     392    1029 
   Size:        392     637
 
SfcI C'TryA_G

Cuts at:      0     203     852    1029 
   Size:        203     649     177

  Fragments arranged by size: 

                 649     203     177
 
StyI C'CwwG_G

Cuts at:      0      96    1029 
   Size:         96     933
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     444    1029 
   Size:        444     585
 
TatI w'GTAC_w

Cuts at:      0     455    1029 
   Size:        455     574
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     207     239     676     708    1029 
   Size:        207      32     437      32     321

  Fragments arranged by size: 

                 437     321     207      32      32
 
XbaI T'CTAG_A

Cuts at:      0     552    1029 
   Size:        552     477
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AlfI    AlwNI     ApoI     BanI    BanII
     BbsI    BciVI    BglII    BmgBI     BmrI     BplI     BpmI     BsaI
    BsaXI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstYI    CspCI
     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI    EcoRV     FalI
    Hin4I  HindIII     MmeI     MscI   MspA1I     NspI    PshAI     PstI
    PvuII     SapI    SexAI     SfcI     StyI    TaqII     TatI     TstI
     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI Bme1580I     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI
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