Restriction map for
pF1KB9474
(Circular) (Six-base) MAPSORT of: pF1KB9474.seq Check: 9908 from: 1 to: 3695
pF1KB9474 3695 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2305 2967 1520
Size: 432 353 662 2248
Fragments arranged by size:
2248 662 432 353
AflIII A'CryG_T
Cuts at: 1047 2847 3128 1047
Size: 1800 281 1614
Fragments arranged by size:
1800 1614 281
AgeI A'CCGG_T
Cuts at: 340 340
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 1000 2438 1000
Size: 1438 2257
ApaLI G'TGCA_C
Cuts at: 2533 2533
Size: 3695
ApoI r'AATT_y
Cuts at: 117 699 3048 3304 117
Size: 582 2349 256 508
Fragments arranged by size:
2349 582 508 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 375 715 375
Size: 340 3355
BamHI G'GATC_C
Cuts at: 720 720
Size: 3695
BanI G'GyrC_C
Cuts at: 711 1125 1373 1408 711
Size: 414 248 35 2998
Fragments arranged by size:
2998 414 248 35
BanII G_rGCy'C
Cuts at: 709 1739 709
Size: 1030 2665
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BbsI GAAGACnn'nnnn_
Cuts at: 439 439
Size: 3695
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2649 3695 1593
Size: 1056 1046 1593
Fragments arranged by size:
1593 1056 1046
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglI GCCn_nnn'nGGC
Cuts at: 136 217 673 136
Size: 81 456 3158
Fragments arranged by size:
3158 456 81
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 1320 1413 2537 1320
Size: 93 1124 2478
Fragments arranged by size:
2478 1124 93
BmgBI CAC'GTC
Cuts at: 125 125
Size: 3695
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BmtI G_CTAG'C
Cuts at: 394 394
Size: 3695
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 871 1750 2464 2762 334
Size: 537 879 714 298 1267
Fragments arranged by size:
1267 879 714 537 298
BsaI GGTCTCn'nnnn_
Cuts at: 3399 3399
Size: 3695
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 1126 1374 2076 3572 1126
Size: 248 702 1496 1249
Fragments arranged by size:
1496 1249 702 248
BsaWI w'CCGG_w
Cuts at: 340 884 1405 2083 2494 2641 2915 340
Size: 544 521 678 411 147 274 1120
Fragments arranged by size:
1120 678 544 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 231 231
Size: 3695
BseYI C'CCAG_C
Cuts at: 1120 1878 2543 2951 1120
Size: 758 665 408 1864
Fragments arranged by size:
1864 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1237 1283 2513 104
Size: 1133 46 1230 1286
Fragments arranged by size:
1286 1230 1133 46
BsiHKAI G_wGCw'C
Cuts at: 709 1487 1677 2537 709
Size: 778 190 860 1867
Fragments arranged by size:
1867 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 709 1320 1413 1487 1677 1739 2537 709
Size: 611 93 74 190 62 798 1867
Fragments arranged by size:
1867 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2127 2127
Size: 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 745 959 1261 1642 745
Size: 214 302 381 2798
Fragments arranged by size:
2798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 340 606 676 1693 1874 340
Size: 266 70 1017 181 2161
Fragments arranged by size:
2161 1017 266 181 70
BssSI C'ACGA_G
Cuts at: 1966 2674 1966
Size: 708 2987
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstEII G'GTnAC_C
Cuts at: 333 333
Size: 3695
BstXI CCAn_nnnn'nTGG
Cuts at: 366 366
Size: 3695
BstYI r'GATC_y
Cuts at: 1 720 1187 1210 1545 1791 2097 2109 2195
Size: 719 467 23 335 246 306 12 86
Cuts at: 2195 3247 1
Size: 1052 449
Fragments arranged by size:
1052 719 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 938 938
Size: 3695
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2745 1401
Size: 1344 2351
EaeI y'GGCC_r
Cuts at: 137 1280 1454 1845 1872 3217 137
Size: 1143 174 391 27 1345 615
Fragments arranged by size:
1345 1143 615 391 174 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 117 1903 2645 2791 3505 117
Size: 1786 742 146 714 307
Fragments arranged by size:
1786 742 714 307 146
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2305 2967 1520
Size: 432 353 662 2248
Fragments arranged by size:
2248 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 371 380 834 371
Size: 9 454 3232
Fragments arranged by size:
3232 454 9
EcoRI G'AATT_C
Cuts at: 117 699 117
Size: 582 3113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3695
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 330 1129 1377 2607 3378 330
Size: 799 248 1230 771 647
Fragments arranged by size:
1230 799 771 647 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1537 1567 1569 1599 1537
Size: 30 2 30 3633
Fragments arranged by size:
3633 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2454 2638 2454
Size: 184 3511
MscI TGG'CCA
Cuts at: 1456 1456
Size: 3695
MslI CAynn'nnrTG
Cuts at: 202 1684 1811 202
Size: 1482 127 2086
Fragments arranged by size:
2086 1482 127
MspA1I CmG'CkG
Cuts at: 806 1120 1480 2264 2509 806
Size: 314 360 784 245 1992
Fragments arranged by size:
1992 784 360 314 245
NaeI GCC'GGC
Cuts at: 678 1876 678
Size: 1198 2497
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NgoMIV G'CCGG_C
Cuts at: 676 1874 676
Size: 1198 2497
NheI G'CTAG_C
Cuts at: 390 390
Size: 3695
NspI r_CATG'y
Cuts at: 748 1248 2851 748
Size: 500 1603 1592
Fragments arranged by size:
1603 1592 500
PciI A'CATG_T
Cuts at: 2847 2847
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 286 318 2141 2173 286
Size: 32 1823 32 1808
Fragments arranged by size:
1823 1808 32 32
PpuMI rG'GwC_Cy
Cuts at: 380 380
Size: 3695
PstI C_TGCA'G
Cuts at: 742 1427 742
Size: 685 3010
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 1120 1480 1120
Size: 360 3335
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 738 1423 2391 2582 33
Size: 705 685 968 191 1146
Fragments arranged by size:
1146 968 705 685 191
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SgrAI Cr'CCGG_yG
Cuts at: 340 340
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 349 850 1765 2479 2741 349
Size: 501 915 714 262 1303
Fragments arranged by size:
1303 915 714 501 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
StuI AGG'CCT
Cuts at: 160 160
Size: 3695
StyI C'CwwG_G
Cuts at: 155 829 1183 155
Size: 674 354 2667
Fragments arranged by size:
2667 674 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 277 1278 1411 1870 2079 127
Size: 150 1001 133 459 209 1743
Fragments arranged by size:
1743 1001 459 209 150 133
TatI w'GTAC_w
Cuts at: 3210 3210
Size: 3695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 3663
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 417 552 1190 327
Size: 90 135 638 2832
Fragments arranged by size:
2832 638 135 90
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI