Restriction map for
pF1KB9465
(Linear) (Six-base) MAPSORT of: KIBB9465.seq Check: 626 from: 1 to: 3111
KIBB9465.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 18 1271 1887 2134 3111
Size: 18 1253 616 247 977
Fragments arranged by size:
1253 977 616 247 18
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1234 1630 2354 3111
Size: 352 882 396 724 757
Fragments arranged by size:
882 757 724 396 352
AflIII A'CryG_T
Cuts at: 0 620 3111
Size: 620 2491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 288 672 1640 3111
Size: 283 5 384 968 1471
Fragments arranged by size:
1471 968 384 283 5
AlwNI CAG_nnn'CTG
Cuts at: 0 1263 1653 2034 3111
Size: 1263 390 381 1077
Fragments arranged by size:
1263 1077 390 381
ApoI r'AATT_y
Cuts at: 0 1020 1291 2695 3111
Size: 1020 271 1404 416
Fragments arranged by size:
1404 1020 416 271
AseI AT'TA_AT
Cuts at: 0 143 3111
Size: 143 2968
AvaI C'yCGr_G
Cuts at: 0 2423 2816 3111
Size: 2423 393 295
Fragments arranged by size:
2423 393 295
BamHI G'GATC_C
Cuts at: 0 2465 3111
Size: 2465 646
BanI G'GyrC_C
Cuts at: 0 30 196 2116 3111
Size: 30 166 1920 995
Fragments arranged by size:
1920 995 166 30
BanII G_rGCy'C
Cuts at: 0 1319 2139 2300 3111
Size: 1319 820 161 811
Fragments arranged by size:
1319 820 811 161
BbsI GAAGACnn'nnnn_
Cuts at: 0 1189 1585 2054 3111
Size: 1189 396 469 1057
Fragments arranged by size:
1189 1057 469 396
BbvCI CC'TCA_GC
Cuts at: 0 903 3044 3111
Size: 903 2141 67
Fragments arranged by size:
2141 903 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2089 2123 2797 2831 3111
Size: 2089 34 674 34 280
Fragments arranged by size:
2089 674 280 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2206 2629 3111
Size: 2206 423 482
Fragments arranged by size:
2206 482 423
BglII A'GATC_T
Cuts at: 0 810 1242 3111
Size: 810 432 1869
Fragments arranged by size:
1869 810 432
BlpI GC'TnA_GC
Cuts at: 0 55 1349 2413 3111
Size: 55 1294 1064 698
Fragments arranged by size:
1294 1064 698 55
Bme1580I G_kGCm'C
Cuts at: 0 35 199 219 3111
Size: 35 164 20 2892
Fragments arranged by size:
2892 164 35 20
BmgBI CAC'GTC
Cuts at: 0 531 1224 3111
Size: 531 693 1887
Fragments arranged by size:
1887 693 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 202 1674 1704 3010 3111
Size: 202 1472 30 1306 101
Fragments arranged by size:
1472 1306 202 101 30
BmtI G_CTAG'C
Cuts at: 0 2738 3111
Size: 2738 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 539 595 810 1558 2032 2043 2236
Size: 103 436 56 215 748 474 11 193
Cuts at: 2236 2515 3111
Size: 279 596
Fragments arranged by size:
748 596 474 436 279 215 193 103
56 11
Bpu10I CC'TnA_GC
Cuts at: 0 391 903 1318 2144 2269 3044 3111
Size: 391 512 415 826 125 775 67
Fragments arranged by size:
826 775 512 415 391 125 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 1568 2254 2889 3111
Size: 1367 201 686 635 222
Fragments arranged by size:
1367 686 635 222 201
BsaHI Gr'CG_yC
Cuts at: 0 1910 2102 3111
Size: 1910 192 1009
Fragments arranged by size:
1910 1009 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1531 1561 1957 1987 2194 2224 2636 2666
Size: 1531 30 396 30 207 30 412 30
Cuts at: 2666 3111
Size: 445
Fragments arranged by size:
1531 445 412 396 207 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 1777 1925 1960 2308 2344 2419 2716
Size: 295 1482 148 35 348 36 75 297
Cuts at: 2716 3111
Size: 395
Fragments arranged by size:
1482 395 348 297 295 148 75 36
35
BseYI C'CCAG_C
Cuts at: 0 76 750 866 1951 2334 2639 2744 2798
Size: 76 674 116 1085 383 305 105 54
Cuts at: 2798 3050 3111
Size: 252 61
Fragments arranged by size:
1085 674 383 305 252 116 105 76
61 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 3111
Size: 435 2676
BsiEI CG_ry'CG
Cuts at: 0 1280 3111
Size: 1280 1831
BsiHKAI G_wGCw'C
Cuts at: 0 272 1319 2300 2510 3111
Size: 272 1047 981 210 601
Fragments arranged by size:
1047 981 601 272 210
BsmI GAATG_Cn'
Cuts at: 0 1470 2244 3111
Size: 1470 774 867
Fragments arranged by size:
1470 867 774
Bsp1286I G_dGCh'C
Cuts at: 0 35 199 219 272 1319 2139 2300 2510
Size: 35 164 20 53 1047 820 161 210
Cuts at: 2510 3111
Size: 601
Fragments arranged by size:
1047 820 601 210 164 161 53 35
20
BspHI T'CATG_A
Cuts at: 0 2721 3111
Size: 2721 390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 494 842 1271 1460 1887 2134 3111
Size: 18 476 348 429 189 427 247 977
Fragments arranged by size:
977 476 429 427 348 247 189 18
BsrBI CCG'CTC
Cuts at: 0 111 629 824 1728 2552 3111
Size: 111 518 195 904 824 559
Fragments arranged by size:
904 824 559 518 195 111
BsrDI GCAATG_nn'
Cuts at: 0 2105 3111
Size: 2105 1006
BsrFI r'CCGG_y
Cuts at: 0 59 2839 3111
Size: 59 2780 272
Fragments arranged by size:
2780 272 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1263 3111
Size: 1263 1848
BstEII G'GTnAC_C
Cuts at: 0 1473 3096 3111
Size: 1473 1623 15
Fragments arranged by size:
1623 1473 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2313 2634 2655 3111
Size: 2313 321 21 456
Fragments arranged by size:
2313 456 321 21
BstYI r'GATC_y
Cuts at: 0 810 860 1242 2465 3111
Size: 810 50 382 1223 646
Fragments arranged by size:
1223 810 646 382 50
BtgI C'CryG_G
Cuts at: 0 1490 1976 2390 3111
Size: 1490 486 414 721
Fragments arranged by size:
1490 721 486 414
BtsI GCAGTG_nn'
Cuts at: 0 40 272 661 1074 2834 3082 3111
Size: 40 232 389 413 1760 248 29
Fragments arranged by size:
1760 413 389 248 232 40 29
DrdI GACnn_nn'nnGTC
Cuts at: 0 228 715 2440 3111
Size: 228 487 1725 671
Fragments arranged by size:
1725 671 487 228
EaeI y'GGCC_r
Cuts at: 0 11 405 1247 1277 1493 1814 2363 2393
Size: 11 394 842 30 216 321 549 30
Cuts at: 2393 3111
Size: 718
Fragments arranged by size:
842 718 549 394 321 216 30 30
11
EagI C'GGCC_G
Cuts at: 0 1277 3111
Size: 1277 1834
EarI CTCTTCn'nnn_
Cuts at: 0 598 733 1784 2216 2876 3111
Size: 598 135 1051 432 660 235
Fragments arranged by size:
1051 660 598 432 235 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 3111
Size: 106 3005
EcoICRI GAG'CTC
Cuts at: 0 1317 2298 3111
Size: 1317 981 813
Fragments arranged by size:
1317 981 813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 352 539 595 810 1234 1558 1630
Size: 103 249 187 56 215 424 324 72
Cuts at: 1630 2032 2043 2236 2354 2515 3111
Size: 402 11 193 118 161 596
Fragments arranged by size:
596 424 402 324 249 215 193 187
161 118 103 72 56 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 982 2491 3111
Size: 394 588 1509 620
Fragments arranged by size:
1509 620 588 394
EcoO109I rG'GnC_Cy
Cuts at: 0 2340 2526 3111
Size: 2340 186 585
Fragments arranged by size:
2340 585 186
EcoRI G'AATT_C
Cuts at: 0 1291 3111
Size: 1291 1820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 744 1140 3111
Size: 744 396 1971
Fragments arranged by size:
1971 744 396
HaeII r_GCGC'y
Cuts at: 0 77 3111
Size: 77 3034
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 940 972 1756 1788 2016 2048 2303 2335
Size: 940 32 784 32 228 32 255 32
Cuts at: 2335 2636 2668 2931 2963 3111
Size: 301 32 263 32 148
Fragments arranged by size:
940 784 301 263 255 228 148 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 240 551 3111
Size: 240 311 2560
Fragments arranged by size:
2560 311 240
HindIII A'AGCT_T
Cuts at: 0 721 3111
Size: 721 2390
HpaI GTT'AAC
Cuts at: 0 551 3111
Size: 551 2560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1529 3111
Size: 1529 1582
MscI TGG'CCA
Cuts at: 0 13 407 1495 1816 3111
Size: 13 394 1088 321 1295
Fragments arranged by size:
1295 1088 394 321 13
MslI CAynn'nnrTG
Cuts at: 0 704 1000 2254 2653 3111
Size: 704 296 1254 399 458
Fragments arranged by size:
1254 704 458 399 296
MspA1I CmG'CkG
Cuts at: 0 411 507 672 754 870 1197 1212 1329
Size: 411 96 165 82 116 327 15 117
Cuts at: 1329 1353 1575 1593 1946 1997 2374 2668 2802
Size: 24 222 18 353 51 377 294 134
Cuts at: 2802 3111
Size: 309
Fragments arranged by size:
411 377 353 327 309 294 222 165
134 117 116 96 82 51 24 18
15
NaeI GCC'GGC
Cuts at: 0 61 2841 3111
Size: 61 2780 270
Fragments arranged by size:
2780 270 61
NcoI C'CATG_G
Cuts at: 0 1490 3111
Size: 1490 1621
NgoMIV G'CCGG_C
Cuts at: 0 59 2839 3111
Size: 59 2780 272
Fragments arranged by size:
2780 272 59
NheI G'CTAG_C
Cuts at: 0 2734 3111
Size: 2734 377
NspI r_CATG'y
Cuts at: 0 624 3111
Size: 624 2487
PasI CC'CwG_GG
Cuts at: 0 2306 3111
Size: 2306 805
PciI A'CATG_T
Cuts at: 0 620 3111
Size: 620 2491
PflMI CCAn_nnn'nTGG
Cuts at: 0 2034 2751 2940 3111
Size: 2034 717 189 171
Fragments arranged by size:
2034 717 189 171
PfoI T'CCnGG_A
Cuts at: 0 1879 3111
Size: 1879 1232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 575 607 1922 1954 3111
Size: 575 32 1315 32 1157
Fragments arranged by size:
1315 1157 575 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2340 2526 3111
Size: 2340 186 585
Fragments arranged by size:
2340 585 186
PstI C_TGCA'G
Cuts at: 0 491 697 839 948 1211 1796 1962 2807
Size: 491 206 142 109 263 585 166 845
Cuts at: 2807 3111
Size: 304
Fragments arranged by size:
845 585 491 304 263 206 166 142
109
PvuII CAG'CTG
Cuts at: 0 411 507 754 870 1212 1593 1946 1997
Size: 411 96 247 116 342 381 353 51
Cuts at: 1997 2374 2668 2802 3111
Size: 377 294 134 309
Fragments arranged by size:
411 381 377 353 342 309 294 247
134 116 96 51
SacI G_AGCT'C
Cuts at: 0 1319 2300 3111
Size: 1319 981 811
Fragments arranged by size:
1319 981 811
SapI GCTCTTCn'nnn_
Cuts at: 0 1784 3111
Size: 1784 1327
ScaI AGT'ACT
Cuts at: 0 841 3111
Size: 841 2270
SexAI A'CCwGG_T
Cuts at: 0 2342 3111
Size: 2342 769
SfcI C'TryA_G
Cuts at: 0 487 693 835 944 1207 1792 1958 2803
Size: 487 206 142 109 263 585 166 845
Cuts at: 2803 3111
Size: 308
Fragments arranged by size:
845 585 487 308 263 206 166 142
109
SmlI C'TyrA_G
Cuts at: 0 1382 1583 2233 2904 3111
Size: 1382 201 650 671 207
Fragments arranged by size:
1382 671 650 207 201
StuI AGG'CCT
Cuts at: 0 952 2170 2812 3111
Size: 952 1218 642 299
Fragments arranged by size:
1218 952 642 299
StyI C'CwwG_G
Cuts at: 0 47 1490 2469 2627 2762 3111
Size: 47 1443 979 158 135 349
Fragments arranged by size:
1443 979 349 158 135 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 900 2179 3111
Size: 214 686 1279 932
Fragments arranged by size:
1279 932 686 214
TatI w'GTAC_w
Cuts at: 0 839 1421 3111
Size: 839 582 1690
Fragments arranged by size:
1690 839 582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 450 482 875 907 1819 1851 1957 1989
Size: 450 32 393 32 912 32 106 32
Cuts at: 1989 3111
Size: 1122
Fragments arranged by size:
1122 912 450 393 106 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 1602 3111
Size: 1602 1509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2079 2655 3111
Size: 2079 576 456
Fragments arranged by size:
2079 576 456
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII
BstXI BstYI BtgI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PpiI PpuMI PstI PvuII SacI SapI ScaI
SexAI SfcI SmlI StuI StyI TaqII TatI TstI
XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BbeI BciVI BclI BfrBI BplI BsaI BsaAI BsaBI
BsaWI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoRV
FseI FspI FspAI KasI KpnI MfeI MluI NarI
NdeI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XhoI XmaI
XmnI ZraI