Restriction map for pF1KB9464
 (Linear) (Six-base) MAPSORT of: KIBB9464.seq  Check: 9384  from: 1  to: 3570



KIBB9464.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AatII G_ACGT'C

Cuts at:      0    1260    3570 
   Size:       1260    2310
 
AccI GT'mk_AC

Cuts at:      0    1901    3570 
   Size:       1901    1669
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1324    1520    1687    2678    3169    3570 
   Size:        364     960     196     167     991     491     401

  Fragments arranged by size: 

                 991     960     491     401     364     196     167
 
AflIII A'CryG_T

Cuts at:      0    1079    2545    3570 
   Size:       1079    1466    1025

  Fragments arranged by size: 

                1466    1079    1025
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2197    2458    2700    3570 
   Size:       2197     261     242     870

  Fragments arranged by size: 

                2197     870     261     242
 
AleI CACnn'nnGTG

Cuts at:      0    2314    3570 
   Size:       2314    1256
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3233    3570 
   Size:       3233     337
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1665    1866    2299    3385    3507    3570 
   Size:       1665     201     433    1086     122      63

  Fragments arranged by size: 

                1665    1086     433     201     122      63
 
ApaI G_GGCC'C

Cuts at:      0     552     819     914    3570 
   Size:        552     267      95    2656

  Fragments arranged by size: 

                2656     552     267      95
 
ApaLI G'TGCA_C

Cuts at:      0    2549    3570 
   Size:       2549    1021
 
ApoI r'AATT_y

Cuts at:      0    2580    3570 
   Size:       2580     990
 
AvaI C'yCGr_G

Cuts at:      0     210     635     695     836     889    1473    3570 
   Size:        210     425      60     141      53     584    2097

  Fragments arranged by size: 

                2097     584     425     210     141      60      53
 
AvrII C'CTAG_G

Cuts at:      0     895    3570 
   Size:        895    2675
 
BanI G'GyrC_C

Cuts at:      0     268    2788    3570 
   Size:        268    2520     782

  Fragments arranged by size: 

                2520     782     268
 
BanII G_rGCy'C

Cuts at:      0     552     819     883     914    2913    3299    3570 
   Size:        552     267      64      31    1999     386     271

  Fragments arranged by size: 

                1999     552     386     271     267      64      31
 
BbeI G_GCGC'C

Cuts at:      0    2792    3570 
   Size:       2792     778
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1881    2117    2699    2774    3570 
   Size:       1881     236     582      75     796

  Fragments arranged by size: 

                1881     796     582     236      75
 
BbvCI CC'TCA_GC

Cuts at:      0     571    3570 
   Size:        571    2999
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1572    1606    3570 
   Size:       1572      34    1964

  Fragments arranged by size: 

                1964    1572      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3447    3570 
   Size:       3447     123
 
BclI T'GATC_A

Cuts at:      0    2520    2682    3570 
   Size:       2520     162     888

  Fragments arranged by size: 

                2520     888     162
 
BglI GCCn_nnn'nGGC

Cuts at:      0     900    3570 
   Size:        900    2670
 
BglII A'GATC_T

Cuts at:      0     977    3570 
   Size:        977    2593
 
Bme1580I G_kGCm'C

Cuts at:      0     552     819     914    2553    3570 
   Size:        552     267      95    1639    1017

  Fragments arranged by size: 

                1639    1017     552     267      95
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2406    3570 
   Size:       2406    1164
 
BmtI G_CTAG'C

Cuts at:      0    2933    3570 
   Size:       2933     637
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622     682    1229    2076    2333    2433    3283    3570 
   Size:        622      60     547     847     257     100     850     287

  Fragments arranged by size: 

                 850     847     622     547     287     257     100      60
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     571    3419    3570 
   Size:        571    2848     151

  Fragments arranged by size: 

                2848     571     151
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1036    1275    3158    3570 
   Size:       1036     239    1883     412

  Fragments arranged by size: 

                1883    1036     412     239
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       8     632     964    1408    1649    2184    2658    3570 
   Size:          8     624     332     444     241     535     474     912

  Fragments arranged by size: 

                 912     624     535     474     444     332     241       8
            
 
BsaHI Gr'CG_yC

Cuts at:      0     534    1257    2789    3570 
   Size:        534     723    1532     781

  Fragments arranged by size: 

                1532     781     723     534
 
BsaWI w'CCGG_w

Cuts at:      0     145     452     581    1568    2321    3570 
   Size:        145     307     129     987     753    1249

  Fragments arranged by size: 

                1249     987     753     307     145     129
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1353    1383    1544    1574    2049    2079    2431    2461 
   Size:       1353      30     161      30     475      30     352      30

Cuts at:   2461    2630    2660    3478    3508    3570 
   Size:        169      30     818      30      62

  Fragments arranged by size: 

                1353     818     475     352     169     161      62      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      26     486    1264    1445    2839    2844    2890    2934 
   Size:         26     460     778     181    1394       5      46      44

Cuts at:   2934    3098    3161    3199    3220    3499    3502    3505    3570 
   Size:        164      63      38      21     279       3       3      65

  Fragments arranged by size: 

                1394     778     460     279     181     164      65      63
                  46      44      38      26      21       5       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     509     883    1449    1923    2035    2148    2893    2964 
   Size:        509     374     566     474     112     113     745      71

Cuts at:   2964    3395    3570 
   Size:        431     175

  Fragments arranged by size: 

                 745     566     509     474     431     374     175     113
                 112      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2887    3570 
   Size:       2887     683
 
BsiEI CG_ry'CG

Cuts at:      0     726     907    3570 
   Size:        726     181    2663

  Fragments arranged by size: 

                2663     726     181
 
BsiHKAI G_wGCw'C

Cuts at:      0     209     866    2553    2913    3299    3570 
   Size:        209     657    1687     360     386     271

  Fragments arranged by size: 

                1687     657     386     360     271     209
 
BsmI GAATG_Cn'

Cuts at:      0    1610    2066    2968    3293    3318    3570 
   Size:       1610     456     902     325      25     252

  Fragments arranged by size: 

                1610     902     456     325     252      25
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1248    3570 
   Size:       1248    2322
 
Bsp1286I G_dGCh'C

Cuts at:      0     209     552     819     866     883     914    2553    2913 
   Size:        209     343     267      47      17      31    1639     360

Cuts at:   2913    3299    3570 
   Size:        386     271

  Fragments arranged by size: 

                1639     386     360     343     271     267     209      47
                  31      17
 
BspEI T'CCGG_A

Cuts at:      0     145     452    3570 
   Size:        145     307    3118

  Fragments arranged by size: 

                3118     307     145
 
BsrBI CCG'CTC

Cuts at:      0     546     564     606     666     786     833    2852    3570 
   Size:        546      18      42      60     120      47    2019     718

  Fragments arranged by size: 

                2019     718     546     120      60      47      42      18
            
 
BsrDI GCAATG_nn'

Cuts at:      0    1590    2234    3570 
   Size:       1590     644    1336

  Fragments arranged by size: 

                1590    1336     644
 
BsrFI r'CCGG_y

Cuts at:      0       3     476    2862    3570 
   Size:          3     473    2386     708

  Fragments arranged by size: 

                2386     708     473       3
 
BssHII G'CGCG_C

Cuts at:      0     628    3570 
   Size:        628    2942
 
BssSI C'ACGA_G

Cuts at:      0    2872    2906    3402    3570 
   Size:       2872      34     496     168

  Fragments arranged by size: 

                2872     496     168      34
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3313    3570 
   Size:       3313     257
 
BstEII G'GTnAC_C

Cuts at:      0    1749    3570 
   Size:       1749    1821
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     685    2077    2316    3570 
   Size:        685    1392     239    1254

  Fragments arranged by size: 

                1392    1254     685     239
 
BstYI r'GATC_y

Cuts at:      0     977    2296    3570 
   Size:        977    1319    1274

  Fragments arranged by size: 

                1319    1274     977
 
Bsu36I CC'TnA_GG

Cuts at:      0    2095    3570 
   Size:       2095    1475
 
BtgI C'CryG_G

Cuts at:      0      18     275     489     588     648     720    2717    3570 
   Size:         18     257     214      99      60      72    1997     853

  Fragments arranged by size: 

                1997     853     257     214      99      72      60      18
            
 
BtsI GCAGTG_nn'

Cuts at:      0     146    3207    3480    3570 
   Size:        146    3061     273      90

  Fragments arranged by size: 

                3061     273     146      90
 
DraIII CAC_nnn'GTG

Cuts at:      0    2414    3570 
   Size:       2414    1156
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     253    3570 
   Size:        253    3317
 
EaeI y'GGCC_r

Cuts at:      0     371     591     651     723     904    2067    3537    3570 
   Size:        371     220      60      72     181    1163    1470      33

  Fragments arranged by size: 

                1470    1163     371     220     181      72      60      33
            
 
EagI C'GGCC_G

Cuts at:      0     723     904    3570 
   Size:        723     181    2666

  Fragments arranged by size: 

                2666     723     181
 
EarI CTCTTCn'nnn_

Cuts at:      0      68     944    3570 
   Size:         68     876    2626

  Fragments arranged by size: 

                2626     876      68
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1645    3570 
   Size:       1645    1925
 
EcoICRI GAG'CTC

Cuts at:      0    2911    3297    3570 
   Size:       2911     386     273

  Fragments arranged by size: 

                2911     386     273
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     622     682    1229    1324    1520    1687    2076 
   Size:        364     258      60     547      95     196     167     389

Cuts at:   2076    2333    2433    2678    3169    3283    3570 
   Size:        257     100     245     491     114     287

  Fragments arranged by size: 

                 547     491     389     364     287     258     257     245
                 196     167     114     100      95      60
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     109     400    3570 
   Size:        109     291    3170

  Fragments arranged by size: 

                3170     291     109
 
EcoO109I rG'GnC_Cy

Cuts at:      0     353     567     609     669     815     841     910     921 
   Size:        353     214      42      60     146      26      69      11

Cuts at:    921    1622    1641    3570 
   Size:        701      19    1929

  Fragments arranged by size: 

                1929     701     353     214     146      69      60      42
                  26      19      11
 
EcoRV GAT'ATC

Cuts at:      0    3348    3570 
   Size:       3348     222
 
HaeII r_GCGC'y

Cuts at:      0    2792    3570 
   Size:       2792     778
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     817     849    1066    1098    1180    1212    1245    1277 
   Size:        817      32     217      32      82      32      33      32

Cuts at:   1277    1353    1385    1871    1903    2088    2120    2183    2215 
   Size:         76      32     486      32     185      32      63      32

Cuts at:   2215    2281    2313    2444    2476    2667    2686    2699    2718 
   Size:         66      32     131      32     191      19      13      19

Cuts at:   2718    3570 
   Size:        852

  Fragments arranged by size: 

                 852     817     486     217     191     185     131      82
                  76      66      63      33      32      32      32      32
                  32      32      32      32      32      32      19      19
                  13
 
HindIII A'AGCT_T

Cuts at:      0    2533    3570 
   Size:       2533    1037
 
KasI G'GCGC_C

Cuts at:      0    2788    3570 
   Size:       2788     782
 
MfeI C'AATT_G

Cuts at:      0     188    2423    3570 
   Size:        188    2235    1147

  Fragments arranged by size: 

                2235    1147     188
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1940    2512    2519    3570 
   Size:       1940     572       7    1051

  Fragments arranged by size: 

                1940    1051     572       7
 
MscI TGG'CCA

Cuts at:      0    2069    3539    3570 
   Size:       2069    1470      31

  Fragments arranged by size: 

                2069    1470      31
 
MslI CAynn'nnrTG

Cuts at:      0     274    2314    3570 
   Size:        274    2040    1256

  Fragments arranged by size: 

                2040    1256     274
 
MspA1I CmG'CkG

Cuts at:      0      20     509     722    2122    2832    3507    3570 
   Size:         20     489     213    1400     710     675      63

  Fragments arranged by size: 

                1400     710     675     489     213      63      20
 
NaeI GCC'GGC

Cuts at:      0     478    3570 
   Size:        478    3092
 
NarI GG'CG_CC

Cuts at:      0    2789    3570 
   Size:       2789     781
 
NcoI C'CATG_G

Cuts at:      0     275     489    2717    3570 
   Size:        275     214    2228     853

  Fragments arranged by size: 

                2228     853     275     214
 
NgoMIV G'CCGG_C

Cuts at:      0     476    3570 
   Size:        476    3094
 
NheI G'CTAG_C

Cuts at:      0    2929    3570 
   Size:       2929     641
 
NspI r_CATG'y

Cuts at:      0    1083    2365    2703    3570 
   Size:       1083    1282     338     867

  Fragments arranged by size: 

                1282    1083     867     338
 
PciI A'CATG_T

Cuts at:      0    1079    3570 
   Size:       1079    2491
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3385    3570 
   Size:       3385     185
 
PfoI T'CCnGG_A

Cuts at:      0    1348    3299    3570 
   Size:       1348    1951     271

  Fragments arranged by size: 

                1951    1348     271
 
PpuMI rG'GwC_Cy

Cuts at:      0     567     841     921    1622    3570 
   Size:        567     274      80     701    1948

  Fragments arranged by size: 

                1948     701     567     274      80
 
PsiI TTA'TAA

Cuts at:      0    1825    3570 
   Size:       1825    1745
 
PspOMI G'GGCC_C

Cuts at:      0     548     815     910    3570 
   Size:        548     267      95    2660

  Fragments arranged by size: 

                2660     548     267      95
 
PvuII CAG'CTG

Cuts at:      0     509    2122    3507    3570 
   Size:        509    1613    1385      63

  Fragments arranged by size: 

                1613    1385     509      63
 
SacI G_AGCT'C

Cuts at:      0    2913    3299    3570 
   Size:       2913     386     271

  Fragments arranged by size: 

                2913     386     271
 
SacII CC_GC'GG

Cuts at:      0      21     723    3570 
   Size:         21     702    2847

  Fragments arranged by size: 

                2847     702      21
 
SanDI GG'GwC_CC

Cuts at:      0     841    3570 
   Size:        841    2729
 
SapI GCTCTTCn'nnn_

Cuts at:      0      68    3570 
   Size:         68    3502
 
SfcI C'TryA_G

Cuts at:      0     462     792    1129    3570 
   Size:        462     330     337    2441

  Fragments arranged by size: 

                2441     462     337     330
 
SfoI GGC'GCC

Cuts at:      0    2790    3570 
   Size:       2790     780
 
SmaI CCC'GGG

Cuts at:      0     212     697    3570 
   Size:        212     485    2873

  Fragments arranged by size: 

                2873     485     212
 
SmlI C'TyrA_G

Cuts at:      0    1051    1290    3173    3570 
   Size:       1051     239    1883     397

  Fragments arranged by size: 

                1883    1051     397     239
 
SphI G_CATG'C

Cuts at:      0    2365    3570 
   Size:       2365    1205
 
SspI AAT'ATT

Cuts at:      0    3251    3570 
   Size:       3251     319
 
StyI C'CwwG_G

Cuts at:      0     275     489     895    2717    2987    3570 
   Size:        275     214     406    1822     270     583

  Fragments arranged by size: 

                1822     583     406     275     270     214
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1345    1380    2425    3570 
   Size:       1345      35    1045    1145

  Fragments arranged by size: 

                1345    1145    1045      35
 
TatI w'GTAC_w

Cuts at:      0      92     803    3570 
   Size:         92     711    2767

  Fragments arranged by size: 

                2767     711      92
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     125     157    1544    1576    2049    2081    3391    3423 
   Size:        125      32    1387      32     473      32    1310      32

Cuts at:   3423    3570 
   Size:        147

  Fragments arranged by size: 

                1387    1310     473     147     125      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     172     393    3231    3570 
   Size:        172     221    2838     339

  Fragments arranged by size: 

                2838     339     221     172
 
XmaI C'CCGG_G

Cuts at:      0     210     695    3570 
   Size:        210     485    2875

  Fragments arranged by size: 

                2875     485     210
 
ZraI GAC'GTC

Cuts at:      0    1258    3570 
   Size:       1258    2312
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    AvrII     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII Bme1580I
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV    HaeII    Hin4I  HindIII     KasI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI
     PciI    PflMI     PfoI    PpuMI     PsiI   PspOMI    PvuII     SacI
    SacII    SanDI     SapI     SfcI     SfoI     SmaI     SmlI     SphI
     SspI     StyI    TaqII     TatI     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AloI     AscI
     AseI    AsiSI     BaeI    BamHI    BfrBI     BlpI    BmgBI     BplI
    BsaAI    BsaBI    BsiWI    BspHI    BspMI    BsrGI    BstBI  BstZ17I
    BtgZI     ClaI    CspCI     DraI    EcoRI     FalI     FseI     FspI
    FspAI   HincII     HpaI     KpnI     MluI     NdeI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI    PspXI
     PsrI     PstI     PvuI    RsrII     SalI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     StuI     SwaI
  Tth111I     XbaI     XhoI     XmnI
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