Restriction map for pF1KB9463
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp700I     AspEI       AspI        BbeI        BglII       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
Bsp1407I    BspEI       BspHI       BsrFI       BsrGI       
BssHII      Cfr10I      CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       EcoT22I     FseI        KasI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmeI        Psp1406I    PvuI        RcaI        
RsrII       SfiI        SfoI        SgrAI       SnaBI       
SpeI        SphI        SspBI       SwaI        Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I       4167  GGTACC               G^GTACC
AcyI         4924  GGCGTC               G[GA]^CG[CT]C
AflII        3771  CTTAAG               C^TTAAG
AseI          367  ATTAAT               AT^TAAT
AsnI          367  ATTAAT               AT^TAAT
Asp718I      4167  GGTACC               G^GTACC
AvrII        3627  CCTAGG               C^CTAGG
BamHI        3572  GGATCC               G^GATCC
BbvCI        4093  GCTGAGG              CCTCAGC(-5/-2)
BciVI        5467  GTATCC               GTATCC(6/5)
BfrI         3771  CTTAAG               C^TTAAG
BglI         2809  GCCAAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3627  CCTAGG               C^CTAGG
BsaHI        4924  GGCGTC               G[GA]^CG[CT]C
BssSI        4308  CACGAG               CACGAG(-5/-1)
Bst1107I     3949  GTATAC               GTA^TAC
BstBI        4547  TTCGAA               TT^CGAA
BstZ17I      3949  GTATAC               GTA^TAC
EcoNI        2741  CCTACCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2826  GAATTC               G^AATTC
Hin1I        4924  GGCGTC               G[GA]^CG[CT]C
HpaI         2260  GTTAAC               GTT^AAC
KpnI         4167  GGTACC               GGTAC^C
KspI         5266  CCGCGG               CCGC^GG
NdeI         1555  CATATG               CA^TATG
NspV         4547  TTCGAA               TT^CGAA
PshAI        4576  GACCAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         367  ATTAAT               AT^TAAT
SacI         5259  GAGCTC               GAGCT^C
SacII        5266  CCGCGG               CCGC^GG
SbfI         3585  CCTGCAGG             CCTGCA^GG
SexAI        2792  ACCAGGT              A^CC[AT]GGT
SfuI         4547  TTCGAA               TT^CGAA
SmaI         1613  CCCGGG               CCC^GGG
SmlI         3771  CTTAAG               C^T[CT][GA]AG
Sse8387I     3585  CCTGCAGG             CCTGCA^GG
XcmI         4307  CCACGAGAGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1613  CCCGGG               C^CCGGG
XmaI         1613  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AviII        1116  TGCGCA               TGC^GCA
             3290  TGCGCA              
BbrPI         226  CACGTG               CAC^GTG
             1030  CACGTG              
BcgI         3007  CGAGTCACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5331  CGATGAAGATGC        
BclI         2157  TGATCA               T^GATCA
             4476  TGATCA              
BsaAI         226  CACGTG               [CT]AC^GT[GA]
             1030  CACGTG              
BsmI         5510  GAATGC               GAATGC(1/-1)
             3185  GCATTC              
BspDI         874  ATCGAT               AT^CGAT
             4405  ATCGAT              
BspLU11I     1305  ACATGT               A^CATGT
             2066  ACATGT              
BstEII       2031  GGTTACC              G^GT[ACGT]ACC
             2457  GGTCACC             
BstPI        2031  GGTTACC              G^GT[ACGT]ACC
             2457  GGTCACC             
ClaI          874  ATCGAT               AT^CGAT
             4405  ATCGAT              
EcoO65I      2031  GGTTACC              G^GT[ACGT]ACC
             2457  GGTCACC             
FbaI         2157  TGATCA               T^GATCA
             4476  TGATCA              
FspI         1116  TGCGCA               TGC^GCA
             3290  TGCGCA              
HaeII        1833  GGCGCT               [GA]GCGC^[CT]
             3029  AGCGCC              
HgaI         2406  GCGTC                GACGC(5/10)
             4925  GCGTC               
PmaCI         226  CACGTG               CAC^GTG
             1030  CACGTG              
PmlI          226  CACGTG               CAC^GTG
             1030  CACGTG              
PpuMI         259  GGGTCCT              [GA]G^G[AT]CC[CT]
             2594  AGGACCT             
SalI         2428  GTCGAC               G^TCGAC
             3911  GTCGAC              
SapI         5233  GAAGAGC              GCTCTTC(1/4)
              298  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
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