Restriction map for pF1KB9463
 (Linear) (Six-base) MAPSORT of: KIBB9463.seq  Check: 2743  from: 1  to: 5616



KIBB9463.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1416    3022    3143    5616 
   Size:       1416    1606     121    2473

  Fragments arranged by size: 

                2473    1606    1416     121
 
AccI GT'mk_AC

Cuts at:      0    2429    3912    3950    5616 
   Size:       2429    1483      38    1666

  Fragments arranged by size: 

                2429    1666    1483      38
 
Acc65I G'GTAC_C

Cuts at:      0    4167    5616 
   Size:       4167    1449
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     619    2263    2458    2688    3175    3359    3630 
   Size:        172     447    1644     195     230     487     184     271

Cuts at:   3630    4473    5048    5495    5616 
   Size:        843     575     447     121

  Fragments arranged by size: 

                1644     843     575     487     447     447     271     230
                 195     184     172     121
 
AflII C'TTAA_G

Cuts at:      0    3771    5616 
   Size:       3771    1845
 
AflIII A'CryG_T

Cuts at:      0    1305    2066    3881    5616 
   Size:       1305     761    1815    1735

  Fragments arranged by size: 

                1815    1735    1305     761
 
AleI CACnn'nnGTG

Cuts at:      0    1133    3523    5616 
   Size:       1133    2390    2093

  Fragments arranged by size: 

                2390    2093    1133
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     734    1625    1772    3541    5616 
   Size:        734     891     147    1769    2075

  Fragments arranged by size: 

                2075    1769     891     734     147
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3269    3301    5616 
   Size:       3269      32    2315

  Fragments arranged by size: 

                3269    2315      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     246    1288    1771    2640    3896    5616 
   Size:        246    1042     483     869    1256    1720

  Fragments arranged by size: 

                1720    1256    1042     869     483     246
 
ApoI r'AATT_y

Cuts at:      0    2826    3214    3356    3682    3985    4086    4535    4544 
   Size:       2826     388     142     326     303     101     449       9

Cuts at:   4544    5616 
   Size:       1072

  Fragments arranged by size: 

                2826    1072     449     388     326     303     142     101
                   9
 
AseI AT'TA_AT

Cuts at:      0     368    5616 
   Size:        368    5248
 
AvaI C'yCGr_G

Cuts at:      0    1415    1613    2927    3431    5616 
   Size:       1415     198    1314     504    2185

  Fragments arranged by size: 

                2185    1415    1314     504     198
 
AvrII C'CTAG_G

Cuts at:      0    3627    5616 
   Size:       3627    1989
 
BamHI G'GATC_C

Cuts at:      0    3572    5616 
   Size:       3572    2044
 
BanI G'GyrC_C

Cuts at:      0     964    1065    1459    3525    4167    5616 
   Size:        964     101     394    2066     642    1449

  Fragments arranged by size: 

                2066    1449     964     642     394     101
 
BanII G_rGCy'C

Cuts at:      0    1185    2966    5240    5263    5616 
   Size:       1185    1781    2274      23     353

  Fragments arranged by size: 

                2274    1781    1185     353      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3401    3537    4895    5616 
   Size:       3401     136    1358     721

  Fragments arranged by size: 

                3401    1358     721     136
 
BbvCI CC'TCA_GC

Cuts at:      0    4094    5616 
   Size:       4094    1522
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2996    3030    5320    5354    5616 
   Size:       2996      34    2290      34     262

  Fragments arranged by size: 

                2996    2290     262      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    5478    5616 
   Size:       5478     138
 
BclI T'GATC_A

Cuts at:      0    2157    4476    5616 
   Size:       2157    2319    1140

  Fragments arranged by size: 

                2319    2157    1140
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2815    5616 
   Size:       2815    2801
 
BlpI GC'TnA_GC

Cuts at:      0    2306    2402    4950    5616 
   Size:       2306      96    2548     666

  Fragments arranged by size: 

                2548    2306     666      96
 
Bme1580I G_kGCm'C

Cuts at:      0     969    1464    2021    2851    3530    3561    5616 
   Size:        969     495     557     830     679      31    2055

  Fragments arranged by size: 

                2055     969     830     679     557     495      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2617    2780    3807    5616 
   Size:       2617     163    1027    1809

  Fragments arranged by size: 

                2617    1809    1027     163
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5277    5616 
   Size:       5277     339
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    1352    1867    2326    4696    5057    5164    5616 
   Size:        496     856     515     459    2370     361     107     452

  Fragments arranged by size: 

                2370     856     515     496     459     452     361     107
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1671    2839    4094    5616 
   Size:       1671    1168    1255    1522

  Fragments arranged by size: 

                1671    1522    1255    1168
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1019    2723    4687    4849    5308    5616 
   Size:       1019    1704    1964     162     459     308

  Fragments arranged by size: 

                1964    1704    1019     459     308     162
 
BsaAI yAC'GTr

Cuts at:      0     228    1032    5616 
   Size:        228     804    4584

  Fragments arranged by size: 

                4584     804     228
 
BsaHI Gr'CG_yC

Cuts at:      0    4925    5616 
   Size:       4925     691
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     758     788    1016    1046    2243    2273    3036    3066 
   Size:        758      30     228      30    1197      30     763      30

Cuts at:   3066    3132    3162    5158    5188    5616 
   Size:         66      30    1996      30     428

  Fragments arranged by size: 

                1996    1197     763     758     428     228      66      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      78     485     794    2467    3137    4543    4810    4843 
   Size:         78     407     309    1673     670    1406     267      33

Cuts at:   4843    5176    5179    5269    5616 
   Size:        333       3      90     347

  Fragments arranged by size: 

                1673    1406     670     407     347     333     309     267
                  90      78      33       3
 
BseYI C'CCAG_C

Cuts at:      0     249    2626    2843    3195    5116    5125    5616 
   Size:        249    2377     217     352    1921       9     491

  Fragments arranged by size: 

                2377    1921     491     352     249     217       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     704     902    1189    1585    3556    4793    5524 
   Size:        259     445     198     287     396    1971    1237     731

Cuts at:   5524    5616 
   Size:         92

  Fragments arranged by size: 

                1971    1237     731     445     396     287     259     198
                  92
 
BsiHKAI G_wGCw'C

Cuts at:      0     472    3114    5249    5263    5616 
   Size:        472    2642    2135      14     353

  Fragments arranged by size: 

                2642    2135     472     353      14
 
BsmI GAATG_Cn'

Cuts at:      0    3185    5516    5616 
   Size:       3185    2331     100

  Fragments arranged by size: 

                3185    2331     100
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1387    2503    4884    5351    5616 
   Size:       1387    1116    2381     467     265

  Fragments arranged by size: 

                2381    1387    1116     467     265
 
Bsp1286I G_dGCh'C

Cuts at:      0     472     969    1185    1464    2021    2851    2966    3114 
   Size:        472     497     216     279     557     830     115     148

Cuts at:   3114    3530    3561    5240    5249    5263    5616 
   Size:        416      31    1679       9      14     353

  Fragments arranged by size: 

                1679     830     557     497     472     416     353     279
                 216     148     115      31      14       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     184    1416    2666    3022    3143    4867    5030    5616 
   Size:        184    1232    1250     356     121    1724     163     586

  Fragments arranged by size: 

                1724    1250    1232     586     356     184     163     121
            
 
BsrBI CCG'CTC

Cuts at:      0     298    1797    2305    5202    5616 
   Size:        298    1499     508    2897     414

  Fragments arranged by size: 

                2897    1499     508     414     298
 
BsrDI GCAATG_nn'

Cuts at:      0      99     305    1208    5616 
   Size:         99     206     903    4408

  Fragments arranged by size: 

                4408     903     206      99
 
BssSI C'ACGA_G

Cuts at:      0    4308    5616 
   Size:       4308    1308
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     246    1771    5022    5511    5616 
   Size:        246    1525    3251     489     105

  Fragments arranged by size: 

                3251    1525     489     246     105
 
BstBI TT'CG_AA

Cuts at:      0    4548    5616 
   Size:       4548    1068
 
BstEII G'GTnAC_C

Cuts at:      0    2031    2457    5616 
   Size:       2031     426    3159

  Fragments arranged by size: 

                3159    2031     426
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     546     672    4761    5616 
   Size:        546     126    4089     855

  Fragments arranged by size: 

                4089     855     546     126
 
BstYI r'GATC_y

Cuts at:      0    3239    3572    3623    3917    5616 
   Size:       3239     333      51     294    1699

  Fragments arranged by size: 

                3239    1699     333     294      51
 
BstZ17I GTA'TAC

Cuts at:      0    3951    5616 
   Size:       3951    1665
 
Bsu36I CC'TnA_GG

Cuts at:      0    2584    2591    2746    5138    5616 
   Size:       2584       7     155    2392     478

  Fragments arranged by size: 

                2584    2392     478     155       7
 
BtgI C'CryG_G

Cuts at:      0    2423    5266    5426    5616 
   Size:       2423    2843     160     190

  Fragments arranged by size: 

                2843    2423     190     160
 
BtsI GCAGTG_nn'

Cuts at:      0     470    1201    1597    1871    2369    2384    3300    3461 
   Size:        470     731     396     274     498      15     916     161

Cuts at:   3461    4805    5616 
   Size:       1344     811

  Fragments arranged by size: 

                1344     916     811     731     498     470     396     274
                 161      15
 
ClaI AT'CG_AT

Cuts at:      0     875    4406    5616 
   Size:        875    3531    1210

  Fragments arranged by size: 

                3531    1210     875
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1289    1324    5616 
   Size:       1289      35    4292

  Fragments arranged by size: 

                4292    1289      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     925    2796    5427    5616 
   Size:        925    1871    2631     189

  Fragments arranged by size: 

                2631    1871     925     189
 
EaeI y'GGCC_r

Cuts at:      0     536    1125    1574    3077    3980    4100    4185    5616 
   Size:        536     589     449    1503     903     120      85    1431

  Fragments arranged by size: 

                1503    1431     903     589     536     449     120      85
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     249     305     755    2537    4394    4574    5024    5228 
   Size:        249      56     450    1782    1857     180     450     204

Cuts at:   5228    5486    5575    5616 
   Size:        258      89      41

  Fragments arranged by size: 

                1857    1782     450     450     258     249     204     180
                  89      56      41
 
EcoICRI GAG'CTC

Cuts at:      0    5261    5616 
   Size:       5261     355
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     496     619    1352    1867    2263    2326    2458 
   Size:        172     324     123     733     515     396      63     132

Cuts at:   2458    2688    3175    3359    3630    4473    4696    5048    5057 
   Size:        230     487     184     271     843     223     352       9

Cuts at:   5057    5164    5495    5616 
   Size:        107     331     121

  Fragments arranged by size: 

                 843     733     515     487     396     352     331     324
                 271     230     223     184     172     132     123     121
                 107      63       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2745    5616 
   Size:       2745    2871
 
EcoO109I rG'GnC_Cy

Cuts at:      0     260    2595    3199    5616 
   Size:        260    2335     604    2417

  Fragments arranged by size: 

                2417    2335     604     260
 
EcoRI G'AATT_C

Cuts at:      0    2826    5616 
   Size:       2826    2790
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2846    4481    5616 
   Size:       2846    1635    1135

  Fragments arranged by size: 

                2846    1635    1135
 
FspI TGC'GCA

Cuts at:      0    1118    3292    5616 
   Size:       1118    2174    2324

  Fragments arranged by size: 

                2324    2174    1118
 
HaeII r_GCGC'y

Cuts at:      0    1837    3033    5616 
   Size:       1837    1196    2583

  Fragments arranged by size: 

                2583    1837    1196
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     601     633     646     678    1152    1184    1621    1653 
   Size:        601      32      13      32     474      32     437      32

Cuts at:   1653    2045    2077    2772    2804    3894    3926    3947    3979 
   Size:        392      32     695      32    1090      32      21      32

Cuts at:   3979    4288    4320    4399    4431    4848    4880    5616 
   Size:        309      32      79      32     417      32     736

  Fragments arranged by size: 

                1090     736     695     601     474     437     417     392
                 309      79      32      32      32      32      32      32
                  32      32      32      32      32      21      13
 
HincII GTy'rAC

Cuts at:      0     738     899    2072    2232    2262    2430    2801    3913 
   Size:        738     161    1173     160      30     168     371    1112

Cuts at:   3913    5529    5616 
   Size:       1616      87

  Fragments arranged by size: 

                1616    1173    1112     738     371     168     161     160
                  87      30
 
HindIII A'AGCT_T

Cuts at:      0      99    2176    3722    5616 
   Size:         99    2077    1546    1894

  Fragments arranged by size: 

                2077    1894    1546      99
 
HpaI GTT'AAC

Cuts at:      0    2262    5616 
   Size:       2262    3354
 
KpnI G_GTAC'C

Cuts at:      0    4171    5616 
   Size:       4171    1445
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     935    2735    3025    3078    5616 
   Size:        935    1800     290      53    2538

  Fragments arranged by size: 

                2538    1800     935     290      53
 
MscI TGG'CCA

Cuts at:      0     538    1127    3079    3982    4102    4187    5616 
   Size:        538     589    1952     903     120      85    1429

  Fragments arranged by size: 

                1952    1429     903     589     538     120      85
 
MslI CAynn'nnrTG

Cuts at:      0     428    1133    1568    2026    2422    3326    3523    4733 
   Size:        428     705     435     458     396     904     197    1210

Cuts at:   4733    5616 
   Size:        883

  Fragments arranged by size: 

                1210     904     883     705     458     435     428     396
                 197
 
MspA1I CmG'CkG

Cuts at:      0    1542    3195    3311    4493    5268    5616 
   Size:       1542    1653     116    1182     775     348

  Fragments arranged by size: 

                1653    1542    1182     775     348     116
 
NdeI CA'TA_TG

Cuts at:      0    1556    5616 
   Size:       1556    4060
 
NspI r_CATG'y

Cuts at:      0     814    1309    1705    2070    4627    5616 
   Size:        814     495     396     365    2557     989

  Fragments arranged by size: 

                2557     989     814     495     396     365
 
PasI CC'CwG_GG

Cuts at:      0    1020    5616 
   Size:       1020    4596
 
PciI A'CATG_T

Cuts at:      0    1305    2066    5616 
   Size:       1305     761    3550

  Fragments arranged by size: 

                3550    1305     761
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     534     942    1830    3039    3896    5616 
   Size:        534     408     888    1209     857    1720

  Fragments arranged by size: 

                1720    1209     888     857     534     408
 
PfoI T'CCnGG_A

Cuts at:      0    2639    3204    3920    4712    5073    5616 
   Size:       2639     565     716     792     361     543

  Fragments arranged by size: 

                2639     792     716     565     543     361
 
PmlI CAC'GTG

Cuts at:      0     228    1032    5616 
   Size:        228     804    4584

  Fragments arranged by size: 

                4584     804     228
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1348    1380    5616 
   Size:       1348      32    4236

  Fragments arranged by size: 

                4236    1348      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     260    2595    5616 
   Size:        260    2335    3021

  Fragments arranged by size: 

                3021    2335     260
 
PshAI GACnn'nnGTC

Cuts at:      0    4580    5616 
   Size:       4580    1036
 
PsiI TTA'TAA

Cuts at:      0    5563    5616 
   Size:       5563      53
 
PstI C_TGCA'G

Cuts at:      0    1355    1776    3590    5160    5616 
   Size:       1355     421    1814    1570     456

  Fragments arranged by size: 

                1814    1570    1355     456     421
 
PvuII CAG'CTG

Cuts at:      0    1542    3195    3311    4493    5616 
   Size:       1542    1653     116    1182    1123

  Fragments arranged by size: 

                1653    1542    1182    1123     116
 
SacI G_AGCT'C

Cuts at:      0    5263    5616 
   Size:       5263     353
 
SacII CC_GC'GG

Cuts at:      0    5269    5616 
   Size:       5269     347
 
SalI G'TCGA_C

Cuts at:      0    2428    3911    5616 
   Size:       2428    1483    1705

  Fragments arranged by size: 

                2428    1705    1483
 
SapI GCTCTTCn'nnn_

Cuts at:      0     305    5228    5616 
   Size:        305    4923     388

  Fragments arranged by size: 

                4923     388     305
 
SbfI CC_TGCA'GG

Cuts at:      0    3590    5616 
   Size:       3590    2026
 
ScaI AGT'ACT

Cuts at:      0    3380    3448    4600    5616 
   Size:       3380      68    1152    1016

  Fragments arranged by size: 

                3380    1152    1016      68
 
SexAI A'CCwGG_T

Cuts at:      0    2792    5616 
   Size:       2792    2824
 
SfcI C'TryA_G

Cuts at:      0     476    1351    1772    3586    3653    4628    5156    5616 
   Size:        476     875     421    1814      67     975     528     460

  Fragments arranged by size: 

                1814     975     875     528     476     460     421      67
            
 
SmaI CCC'GGG

Cuts at:      0    1615    5616 
   Size:       1615    4001
 
SmlI C'TyrA_G

Cuts at:      0    3771    5616 
   Size:       3771    1845
 
SspI AAT'ATT

Cuts at:      0     366     442    5059    5616 
   Size:        366      76    4617     557

  Fragments arranged by size: 

                4617     557     366      76
 
StuI AGG'CCT

Cuts at:      0    2589    3074    3584    4316    5616 
   Size:       2589     485     510     732    1300

  Fragments arranged by size: 

                2589    1300     732     510     485
 
StyI C'CwwG_G

Cuts at:      0     215    2390    2810    3120    3219    3627    4482    5616 
   Size:        215    2175     420     310      99     408     855    1134

  Fragments arranged by size: 

                2175    1134     855     420     408     310     215      99
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2599    5616 
   Size:       2599    3017
 
TatI w'GTAC_w

Cuts at:      0    2992    3378    3446    3507    4598    5616 
   Size:       2992     386      68      61    1091    1018

  Fragments arranged by size: 

                2992    1091    1018     386      68      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     758     790    1016    1048    5411    5443    5616 
   Size:        758      32     226      32    4363      32     173

  Fragments arranged by size: 

                4363     758     226     173      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4314    5616 
   Size:       4314    1302
 
XmaI C'CCGG_G

Cuts at:      0    1613    5616 
   Size:       1613    4003
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AleI     AlfI
     AloI    AlwNI     ApoI     AseI     AvaI    AvrII    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MmeI
     MscI     MslI   MspA1I     NdeI     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI     PsiI     PstI    PvuII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SmaI     SmlI     SspI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AgeI     AhdI     ApaI    ApaLI     AscI
    AsiSI     BaeI     BbeI    BfrBI    BglII    BmgBI     BmtI    BpuEI
    BsaBI    BsaWI    BsiEI    BsiWI    BspEI    BspHI    BsrFI    BsrGI
   BssHII    BtgZI     DraI     DrdI     EagI     EciI    EcoRV     FseI
    FspAI     KasI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PmeI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SanDI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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