Restriction map for pF1KB9462
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
AocI        AscI        AseI        AsnI        Asp700I     
AspEI       AvrII       BamHI       BcgI        BciVI       
BglII       BlnI        BsaWI       BseAI       BsmBI       
BspEI       BspLU11I    BsrDI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      CpoI        DraI        DrdI        Eam1105I    
Eco81I      EcoO65I     EcoRV       EcoT22I     FseI        
HpaI        KspI        MfeI        MflI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PinAI       PmeI        
PpuMI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        XbaI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1640  GGTACC               G^GTACC
AccI         2524  GTCTAC               GT^[AC][GT]AC
AcsI          120  GAATTC               [GA]^AATT[CT]
AcyI          304  GGCGCC               G[GA]^CG[CT]C
ApaI           32  GGGCCC               GGGCC^C
ApoI          120  GAATTC               [GA]^AATT[CT]
Asp718I      1640  GGTACC               G^GTACC
AspI          631  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1445  TGCGCA               TGC^GCA
BbeI          304  GGCGCC               GGCGC^C
BbrPI        2295  CACGTG               CAC^GTG
BbsI         1590  GAAGAC               GAAGAC(2/6)
BclI         1817  TGATCA               T^GATCA
BfaI         1981  CTAG                 C^TAG
BpuAI        1590  GAAGAC               GAAGAC(2/6)
BsaBI        1986  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         304  GGCGCC               G[GA]^CG[CT]C
BsiWI        1020  CGTACG               C^GTACG
BspDI        1228  ATCGAT               AT^CGAT
BspHI        1925  TCATGA               T^CATGA
BstBI         825  TTCGAA               TT^CGAA
BstXI         485  CCATCATGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         1228  ATCGAT               AT^CGAT
DraII        2681  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII       2170  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I     2681  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         120  GAATTC               G^AATTC
FbaI         1817  TGATCA               T^GATCA
FspI         1445  TGCGCA               TGC^GCA
HgaI         2460  GACGC                GACGC(5/10)
Hin1I         304  GGCGCC               G[GA]^CG[CT]C
HincII       1357  GTCAAC               GT[CT]^[GA]AC
HindII       1357  GTCAAC               GT[CT]^[GA]AC
HindIII      1738  AAGCTT               A^AGCTT
KasI          304  GGCGCC               G^GCGCC
KpnI         1640  GGTACC               GGTAC^C
MaeI         1981  CTAG                 C^TAG
MamI         1986  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1718  GCCGGC               GCC^GGC
NarI          304  GGCGCC               GG^CGCC
NgoMIV       1718  GCCGGC               G^CCGGC
NspV          825  TTCGAA               TT^CGAA
PaeR7I        346  CTCGAG               C^TCGAG
PflFI         631  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI         2478  GAGTC                GAGTC(4/5)
PmaCI        2295  CACGTG               CAC^GTG
PmlI         2295  CACGTG               CAC^GTG
RcaI         1925  TCATGA               T^CATGA
SexAI        1649  ACCAGGT              A^CC[AT]GGT
SfoI          304  GGCGCC               GGC^GCC
SfuI          825  TTCGAA               TT^CGAA
Tth111I       631  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          346  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflII         208  CTTAAG               C^TTAAG
             1339  CTTAAG              
Alw44I       1161  GTGCAC               G^TGCAC
             2298  GTGCAC              
AlwI          999  GATCC                GGATC(4/5)
              258  GGATC               
Aor51HI      2054  AGCGCT               AGC^GCT
             2688  AGCGCT              
ApaLI        1161  GTGCAC               G^TGCAC
             2298  GTGCAC              
BbvCI          57  CCTCAGC              CCTCAGC(-5/-2)
              982  GCTGAGG             
BfrI          208  CTTAAG               C^TTAAG
             1339  CTTAAG              
BglI           62  GCCTTTACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2264  GCCTGCTGGGC         
BlpI         1119  GCTGAGC              GC^T[ACGT]AGC
             1933  GCTGAGC             
Bpu1102I     1119  GCTGAGC              GC^T[ACGT]AGC
             1933  GCTGAGC             
BseRI          55  CTCCTC               GAGGAG(10/8)
              973  GAGGAG              
BsmI         2504  GAATGC               GAATGC(1/-1)
              311  GCATTC              
Bsp1407I     2372  TGTACA               T^GTACA
             2444  TGTACA              
BsrGI        2372  TGTACA               T^GTACA
             2444  TGTACA              
CelII        1119  GCTGAGC              GC^T[ACGT]AGC
             1933  GCTGAGC             
EagI         1287  CGGCCG               C^GGCCG
             2432  CGGCCG              
EclXI        1287  CGGCCG               C^GGCCG
             2432  CGGCCG              
Eco47III     2054  AGCGCT               AGC^GCT
             2688  AGCGCT              
Eco52I       1287  CGGCCG               C^GGCCG
             2432  CGGCCG              
MseI          209  TTAA                 T^TAA
             1340  TTAA                
NcoI          623  CCATGG               C^CATGG
             1244  CCATGG              
PshAI        1276  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2706  GACTTACGTC          
PstI         1557  CTGCAG               CTGCA^G
             2125  CTGCAG              
SacI          474  GAGCTC               GAGCT^C
             2662  GAGCTC              
SphI         1604  GCATGC               GCATG^C
             2279  GCATGC              
SspBI        2372  TGTACA               T^GTACA
             2444  TGTACA              
Tru9I         209  TTAA                 T^TAA
             1340  TTAA                
Tsp509I       121  AATT                 ^AATT
             1179  AATT                
XcmI         1046  CCAGCCGCACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1802  CCACGTTGTTCGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaAI       BsaI        BsaJI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EarI        EcoNI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      
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