Restriction map for pF1KB9462
 (Linear) (Six-base) MAPSORT of: KIBB9462.seq  Check: 592  from: 1  to: 2736



KIBB9462.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     331    1502    1960    2736 
   Size:        331    1171     458     776

  Fragments arranged by size: 

                1171     776     458     331
 
AccI GT'mk_AC

Cuts at:      0    2525    2736 
   Size:       2525     211
 
Acc65I G'GTAC_C

Cuts at:      0    1640    2736 
   Size:       1640    1096
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452    2094    2736 
   Size:        452    1642     642

  Fragments arranged by size: 

                1642     642     452
 
AfeI AGC'GCT

Cuts at:      0    2056    2690    2736 
   Size:       2056     634      46

  Fragments arranged by size: 

                2056     634      46
 
AflII C'TTAA_G

Cuts at:      0     208    1339    2736 
   Size:        208    1131    1397

  Fragments arranged by size: 

                1397    1131     208
 
AflIII A'CryG_T

Cuts at:      0     444     605    1675    2736 
   Size:        444     161    1070    1061

  Fragments arranged by size: 

                1070    1061     444     161
 
AleI CACnn'nnGTG

Cuts at:      0    1825    2736 
   Size:       1825     911
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1351    2736 
   Size:       1351    1385
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     646     678    2736 
   Size:        646      32    2058

  Fragments arranged by size: 

                2058     646      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     357    1542    2133    2736 
   Size:        357    1185     591     603

  Fragments arranged by size: 

                1185     603     591     357
 
ApaI G_GGCC'C

Cuts at:      0      36    2736 
   Size:         36    2700
 
ApaLI G'TGCA_C

Cuts at:      0    1161    2298    2736 
   Size:       1161    1137     438

  Fragments arranged by size: 

                1161    1137     438
 
ApoI r'AATT_y

Cuts at:      0     120    2736 
   Size:        120    2616
 
AvaI C'yCGr_G

Cuts at:      0     346    1860    2332    2736 
   Size:        346    1514     472     404

  Fragments arranged by size: 

                1514     472     404     346
 
BanI G'GyrC_C

Cuts at:      0     304     594    1327    1640    1948    2419    2736 
   Size:        304     290     733     313     308     471     317

  Fragments arranged by size: 

                 733     471     317     313     308     304     290
 
BanII G_rGCy'C

Cuts at:      0      36     184     478     779     931     946    1301    1753 
   Size:         36     148     294     301     152      15     355     452

Cuts at:   1753    1940    2666    2736 
   Size:        187     726      70

  Fragments arranged by size: 

                 726     452     355     301     294     187     152     148
                  70      36      15
 
BbeI G_GCGC'C

Cuts at:      0     308    2736 
   Size:        308    2428
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1597    2736 
   Size:       1597    1139
 
BbvCI CC'TCA_GC

Cuts at:      0      58     983    2736 
   Size:         58     925    1753

  Fragments arranged by size: 

                1753     925      58
 
BclI T'GATC_A

Cuts at:      0    1817    2736 
   Size:       1817     919
 
BglI GCCn_nnn'nGGC

Cuts at:      0      68    2270    2736 
   Size:         68    2202     466

  Fragments arranged by size: 

                2202     466      68
 
BlpI GC'TnA_GC

Cuts at:      0    1120    1934    2736 
   Size:       1120     814     802

  Fragments arranged by size: 

                1120     814     802
 
Bme1580I G_kGCm'C

Cuts at:      0      36     599     645    1165    2070    2302    2333    2561 
   Size:         36     563      46     520     905     232      31     228

Cuts at:   2561    2736 
   Size:        175

  Fragments arranged by size: 

                 905     563     520     232     228     175      46      36
                  31
 
BmgBI CAC'GTC

Cuts at:      0    1152    1676    2736 
   Size:       1152     524    1060

  Fragments arranged by size: 

                1152    1060     524
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     160     747    2736 
   Size:        160     587    1989

  Fragments arranged by size: 

                1989     587     160
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1767    2736 
   Size:       1767     969
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     312     838     901     991    1029    2736 
   Size:        256      56     526      63      90      38    1707

  Fragments arranged by size: 

                1707     526     256      90      63      56      38
 
Bpu10I CC'TnA_GC

Cuts at:      0      58     983    2736 
   Size:         58     925    1753

  Fragments arranged by size: 

                1753     925      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     631    1120    1762    2215    2698    2736 
   Size:        631     489     642     453     483      38

  Fragments arranged by size: 

                 642     631     489     483     453      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     486    2329    2475    2736 
   Size:        486    1843     146     261

  Fragments arranged by size: 

                1843     486     261     146
 
BsaAI yAC'GTr

Cuts at:      0     608    1881    2297    2736 
   Size:        608    1273     416     439

  Fragments arranged by size: 

                1273     608     439     416
 
BsaBI GATnn'nnATC

Cuts at:      0    1990    2736 
   Size:       1990     746
 
BsaHI Gr'CG_yC

Cuts at:      0     305    2736 
   Size:        305    2431
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     568     598    2736 
   Size:        310      30     228      30    2138

  Fragments arranged by size: 

                2138     310     228      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     988    2736 
   Size:         46     942    1748

  Fragments arranged by size: 

                1748     942      46
 
BseYI C'CCAG_C

Cuts at:      0     300     938    1331    2133    2268    2685    2736 
   Size:        300     638     393     802     135     417      51

  Fragments arranged by size: 

                 802     638     417     393     300     135      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     949    1237    1243    1480    2736 
   Size:        388     561     288       6     237    1256

  Fragments arranged by size: 

                1256     561     388     288     237       6
 
BsiEI CG_ry'CG

Cuts at:      0    1035    1290    2435    2736 
   Size:       1035     255    1145     301

  Fragments arranged by size: 

                1145    1035     301     255
 
BsiHKAI G_wGCw'C

Cuts at:      0     478    1165    2231    2302    2666    2736 
   Size:        478     687    1066      71     364      70

  Fragments arranged by size: 

                1066     687     478     364      71      70
 
BsiWI C'GTAC_G

Cuts at:      0    1020    2736 
   Size:       1020    1716
 
BsmI GAATG_Cn'

Cuts at:      0     311    2510    2736 
   Size:        311    2199     226

  Fragments arranged by size: 

                2199     311     226
 
Bsp1286I G_dGCh'C

Cuts at:      0      36     184     478     599     645     779     931     946 
   Size:         36     148     294     121      46     134     152      15

Cuts at:    946    1165    1301    1753    1940    2070    2231    2302    2333 
   Size:        219     136     452     187     130     161      71      31

Cuts at:   2333    2561    2666    2736 
   Size:        228     105      70

  Fragments arranged by size: 

                 452     294     228     219     187     161     152     148
                 136     134     130     121     105      71      70      46
                  36      31      15
 
BspHI T'CATG_A

Cuts at:      0    1925    2736 
   Size:       1925     811
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     290     331    1502    1960    2736 
   Size:        290      41    1171     458     776

  Fragments arranged by size: 

                1171     776     458     290      41
 
BsrBI CCG'CTC

Cuts at:      0     698    1512    1532    1550    1584    2736 
   Size:        698     814      20      18      34    1152

  Fragments arranged by size: 

                1152     814     698      34      20      18
 
BsrFI r'CCGG_y

Cuts at:      0     230    1067    1718    2736 
   Size:        230     837     651    1018

  Fragments arranged by size: 

                1018     837     651     230
 
BsrGI T'GTAC_A

Cuts at:      0    2372    2444    2736 
   Size:       2372      72     292

  Fragments arranged by size: 

                2372     292      72
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1266    1542    1610    1715    2556    2736 
   Size:       1266     276      68     105     841     180

  Fragments arranged by size: 

                1266     841     276     180     105      68
 
BstBI TT'CG_AA

Cuts at:      0     826    2736 
   Size:        826    1910
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     492    2736 
   Size:        492    2244
 
BtgI C'CryG_G

Cuts at:      0     623     769    1244    1784    2736 
   Size:        623     146     475     540     952

  Fragments arranged by size: 

                 952     623     540     475     146
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     732    1356    1779    2736 
   Size:        732     624     423     957

  Fragments arranged by size: 

                 957     732     624     423
 
BtsI GCAGTG_nn'

Cuts at:      0     417    1110    1620    2736 
   Size:        417     693     510    1116

  Fragments arranged by size: 

                1116     693     510     417
 
ClaI AT'CG_AT

Cuts at:      0    1229    2736 
   Size:       1229    1507
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     180     215    2736 
   Size:        180      35    2521

  Fragments arranged by size: 

                2521     180      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2175    2736 
   Size:       2175     561
 
EaeI y'GGCC_r

Cuts at:      0     174     494    1287    1522    1781    2099    2432    2597 
   Size:        174     320     793     235     259     318     333     165

Cuts at:   2597    2693    2736 
   Size:         96      43

  Fragments arranged by size: 

                 793     333     320     318     259     235     174     165
                  96      43
 
EagI C'GGCC_G

Cuts at:      0    1287    2432    2736 
   Size:       1287    1145     304

  Fragments arranged by size: 

                1287    1145     304
 
EarI CTCTTCn'nnn_

Cuts at:      0       4     517    1780    2542    2736 
   Size:          4     513    1263     762     194

  Fragments arranged by size: 

                1263     762     513     194       4
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     796    2736 
   Size:        796    1940
 
EcoICRI GAG'CTC

Cuts at:      0     476    2664    2736 
   Size:        476    2188      72

  Fragments arranged by size: 

                2188     476      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     312     452     838     901     991    1029    2094 
   Size:        256      56     140     386      63      90      38    1065

Cuts at:   2094    2736 
   Size:        642

  Fragments arranged by size: 

                1065     642     386     256     140      90      63      56
                  38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     327     973    1138    1634    2736 
   Size:        327     646     165     496    1102

  Fragments arranged by size: 

                1102     646     496     327     165
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2682    2736 
   Size:       2682      54
 
EcoRI G'AATT_C

Cuts at:      0     120    2736 
   Size:        120    2616
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      33    2649    2736 
   Size:         33    2616      87

  Fragments arranged by size: 

                2616      87      33
 
FspI TGC'GCA

Cuts at:      0    1447    2736 
   Size:       1447    1289
 
HaeII r_GCGC'y

Cuts at:      0     308     376     550    1422    2058    2489    2692    2736 
   Size:        308      68     174     872     636     431     203      44

  Fragments arranged by size: 

                 872     636     431     308     203     174      68      44
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     568     600    2641    2673    2736 
   Size:        310      32     226      32    2041      32      63

  Fragments arranged by size: 

                2041     310     226      63      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1359    2736 
   Size:       1359    1377
 
HindIII A'AGCT_T

Cuts at:      0    1738    2736 
   Size:       1738     998
 
KasI G'GCGC_C

Cuts at:      0     304    2736 
   Size:        304    2432
 
KpnI G_GTAC'C

Cuts at:      0    1644    2736 
   Size:       1644    1092
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2633    2736 
   Size:       2633     103
 
MscI TGG'CCA

Cuts at:      0     496    2101    2695    2736 
   Size:        496    1605     594      41

  Fragments arranged by size: 

                1605     594     496      41
 
MslI CAynn'nnrTG

Cuts at:      0     490    1825    2143    2736 
   Size:        490    1335     318     593

  Fragments arranged by size: 

                1335     593     490     318
 
MspA1I CmG'CkG

Cuts at:      0     404     938    1335    1443    1542    2097    2130    2196 
   Size:        404     534     397     108      99     555      33      66

Cuts at:   2196    2736 
   Size:        540

  Fragments arranged by size: 

                 555     540     534     404     397     108      99      66
                  33
 
NaeI GCC'GGC

Cuts at:      0    1720    2736 
   Size:       1720    1016
 
NarI GG'CG_CC

Cuts at:      0     305    2736 
   Size:        305    2431
 
NcoI C'CATG_G

Cuts at:      0     623    1244    2736 
   Size:        623     621    1492

  Fragments arranged by size: 

                1492     623     621
 
NgoMIV G'CCGG_C

Cuts at:      0    1718    2736 
   Size:       1718    1018
 
NspI r_CATG'y

Cuts at:      0    1608    1692    2283    2517    2736 
   Size:       1608      84     591     234     219

  Fragments arranged by size: 

                1608     591     234     219      84
 
PasI CC'CwG_GG

Cuts at:      0      90    1757    2499    2736 
   Size:         90    1667     742     237

  Fragments arranged by size: 

                1667     742     237      90
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     234     624    2175    2736 
   Size:        234     390    1551     561

  Fragments arranged by size: 

                1551     561     390     234
 
PfoI T'CCnGG_A

Cuts at:      0     815    1480    2186    2736 
   Size:        815     665     706     550

  Fragments arranged by size: 

                 815     706     665     550
 
PmlI CAC'GTG

Cuts at:      0    2297    2736 
   Size:       2297     439
 
PshAI GACnn'nnGTC

Cuts at:      0    1280    2710    2736 
   Size:       1280    1430      26

  Fragments arranged by size: 

                1430    1280      26
 
PspOMI G'GGCC_C

Cuts at:      0      32    2736 
   Size:         32    2704
 
PspXI vC'TCGA_Gb

Cuts at:      0     346    2736 
   Size:        346    2390
 
PstI C_TGCA'G

Cuts at:      0    1561    2129    2736 
   Size:       1561     568     607

  Fragments arranged by size: 

                1561     607     568
 
PvuII CAG'CTG

Cuts at:      0     938    1335    1443    1542    2097    2130    2736 
   Size:        938     397     108      99     555      33     606

  Fragments arranged by size: 

                 938     606     555     397     108      99      33
 
SacI G_AGCT'C

Cuts at:      0     478    2666    2736 
   Size:        478    2188      70

  Fragments arranged by size: 

                2188     478      70
 
SexAI A'CCwGG_T

Cuts at:      0    1649    2736 
   Size:       1649    1087
 
SfcI C'TryA_G

Cuts at:      0    1319    1557    2125    2274    2736 
   Size:       1319     238     568     149     462

  Fragments arranged by size: 

                1319     568     462     238     149
 
SfoI GGC'GCC

Cuts at:      0     306    2736 
   Size:        306    2430
 
SmlI C'TyrA_G

Cuts at:      0     208     346     646    1135    1339    1741    2230    2677 
   Size:        208     138     300     489     204     402     489     447

Cuts at:   2677    2736 
   Size:         59

  Fragments arranged by size: 

                 489     489     447     402     300     208     204     138
                  59
 
SphI G_CATG'C

Cuts at:      0    1608    2283    2736 
   Size:       1608     675     453

  Fragments arranged by size: 

                1608     675     453
 
StuI AGG'CCT

Cuts at:      0     158     709    1858    2736 
   Size:        158     551    1149     878

  Fragments arranged by size: 

                1149     878     551     158
 
StyI C'CwwG_G

Cuts at:      0     623     779    1244    1944    2736 
   Size:        623     156     465     700     792

  Fragments arranged by size: 

                 792     700     623     465     156
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     352     438     442     468    1345    2049    2736 
   Size:        352      86       4      26     877     704     687

  Fragments arranged by size: 

                 877     704     687     352      86      26       4
 
TatI w'GTAC_w

Cuts at:      0    1343    1463    2372    2444    2736 
   Size:       1343     120     909      72     292

  Fragments arranged by size: 

                1343     909     292     120      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     106     138    2736 
   Size:        106      32    2598

  Fragments arranged by size: 

                2598     106      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     634    2736 
   Size:        634    2102
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1053    1809    2736 
   Size:       1053     756     927

  Fragments arranged by size: 

                1053     927     756
 
XhoI C'TCGA_G

Cuts at:      0     346    2736 
   Size:        346    2390
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BstAPI    BstBI    BstXI     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI    PshAI
   PspOMI    PspXI     PstI    PvuII     SacI    SexAI     SfcI     SfoI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AclI     AgeI     AhdI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BciVI    BfrBI    BglII     BmtI    BsaWI
    BsmBI    BspEI    BsrDI   BssHII    BssSI   BstEII    BstYI  BstZ17I
   Bsu36I     DraI     DrdI    EcoRV     FseI    FspAI     HpaI     MfeI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PpiI    PpuMI     PsiI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XmaI
     XmnI     ZraI
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