Restriction map for pF1KB9461
 (Linear) (Six-base) MAPSORT of: KIBB9461.seq  Check: 4174  from: 1  to: 1674



KIBB9461.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     970    1181    1674 
   Size:        970     211     493

  Fragments arranged by size: 

                 970     493     211
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544     637    1674 
   Size:        544      93    1037

  Fragments arranged by size: 

                1037     544      93
 
AflII C'TTAA_G

Cuts at:      0    1180    1674 
   Size:       1180     494
 
AflIII A'CryG_T

Cuts at:      0     269    1674 
   Size:        269    1405
 
AleI CACnn'nnGTG

Cuts at:      0    1375    1674 
   Size:       1375     299
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1271    1303    1674 
   Size:       1271      32     371

  Fragments arranged by size: 

                1271     371      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     633    1674 
   Size:        633    1041
 
ApaLI G'TGCA_C

Cuts at:      0    1464    1674 
   Size:       1464     210
 
ApoI r'AATT_y

Cuts at:      0     365     670     700     874    1234    1674 
   Size:        365     305      30     174     360     440

  Fragments arranged by size: 

                 440     365     360     305     174      30
 
AvaI C'yCGr_G

Cuts at:      0     919    1674 
   Size:        919     755
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1594    1627    1674 
   Size:       1594      33      47

  Fragments arranged by size: 

                1594      47      33
 
BamHI G'GATC_C

Cuts at:      0     164    1674 
   Size:        164    1510
 
BanI G'GyrC_C

Cuts at:      0     694    1674 
   Size:        694     980
 
BanII G_rGCy'C

Cuts at:      0      67    1674 
   Size:         67    1607
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     676     710    1674 
   Size:        676      34     964

  Fragments arranged by size: 

                 964     676      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     429    1674 
   Size:        429    1245
 
BclI T'GATC_A

Cuts at:      0     506    1674 
   Size:        506    1168
 
BglII A'GATC_T

Cuts at:      0    1001    1674 
   Size:       1001     673
 
Bme1580I G_kGCm'C

Cuts at:      0    1468    1674 
   Size:       1468     206
 
BmtI G_CTAG'C

Cuts at:      0    1424    1674 
   Size:       1424     250
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     553     675    1511    1674 
   Size:        239     314     122     836     163

  Fragments arranged by size: 

                 836     314     239     163     122
 
Bpu10I CC'TnA_GC

Cuts at:      0     496    1674 
   Size:        496    1178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703    1674 
   Size:        703     971
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     349     729    1674 
   Size:        349     380     945

  Fragments arranged by size: 

                 945     380     349
 
BsaWI w'CCGG_w

Cuts at:      0    1481    1674 
   Size:       1481     193
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88      91    1674 
   Size:         88       3    1583

  Fragments arranged by size: 

                1583      88       3
 
BseYI C'CCAG_C

Cuts at:      0    1341    1506    1674 
   Size:       1341     165     168

  Fragments arranged by size: 

                1341     168     165
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1607    1674 
   Size:       1607      67
 
BsiHKAI G_wGCw'C

Cuts at:      0      67    1468    1674 
   Size:         67    1401     206

  Fragments arranged by size: 

                1401     206      67
 
Bsp1286I G_dGCh'C

Cuts at:      0      67    1468    1674 
   Size:         67    1401     206

  Fragments arranged by size: 

                1401     206      67
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     970    1181    1259    1674 
   Size:        970     211      78     415

  Fragments arranged by size: 

                 970     415     211      78
 
BsrDI GCAATG_nn'

Cuts at:      0     605    1674 
   Size:        605    1069
 
BsrFI r'CCGG_y

Cuts at:      0    1241    1674 
   Size:       1241     433
 
BsrGI T'GTAC_A

Cuts at:      0     272    1571    1674 
   Size:        272    1299     103

  Fragments arranged by size: 

                1299     272     103
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     705    1674 
   Size:        705     969
 
BstYI r'GATC_y

Cuts at:      0     164     343    1001    1674 
   Size:        164     179     658     673

  Fragments arranged by size: 

                 673     658     179     164
 
BtgI C'CryG_G

Cuts at:      0    1076    1674 
   Size:       1076     598
 
BtsI GCAGTG_nn'

Cuts at:      0     428    1674 
   Size:        428    1246
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     471     506    1335    1370    1674 
   Size:        471      35     829      35     304

  Fragments arranged by size: 

                 829     471     304      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1463    1674 
   Size:       1463     211
 
EaeI y'GGCC_r

Cuts at:      0       2    1674 
   Size:          2    1672
 
EarI CTCTTCn'nnn_

Cuts at:      0      65    1109    1674 
   Size:         65    1044     565

  Fragments arranged by size: 

                1044     565      65
 
EcoICRI GAG'CTC

Cuts at:      0      65    1674 
   Size:         65    1609
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     544     553     637     675    1511    1674 
   Size:        239     305       9      84      38     836     163

  Fragments arranged by size: 

                 836     305     239     163      84      38       9
 
EcoRI G'AATT_C

Cuts at:      0     365     874    1674 
   Size:        365     509     800

  Fragments arranged by size: 

                 800     509     365
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     423     445     455     477    1674 
   Size:        423      22      10      22    1197

  Fragments arranged by size: 

                1197     423      22      22      10
 
HincII GTy'rAC

Cuts at:      0     464    1674 
   Size:        464    1210
 
HindIII A'AGCT_T

Cuts at:      0    1576    1619    1674 
   Size:       1576      43      55

  Fragments arranged by size: 

                1576      55      43
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     279    1241    1619    1674 
   Size:        279     962     378      55

  Fragments arranged by size: 

                 962     378     279      55
 
MscI TGG'CCA

Cuts at:      0       4    1674 
   Size:          4    1670
 
MslI CAynn'nnrTG

Cuts at:      0      57    1375    1674 
   Size:         57    1318     299

  Fragments arranged by size: 

                1318     299      57
 
NaeI GCC'GGC

Cuts at:      0    1243    1674 
   Size:       1243     431
 
NdeI CA'TA_TG

Cuts at:      0      54     678    1374    1674 
   Size:         54     624     696     300

  Fragments arranged by size: 

                 696     624     300      54
 
NgoMIV G'CCGG_C

Cuts at:      0    1241    1674 
   Size:       1241     433
 
NheI G'CTAG_C

Cuts at:      0    1420    1674 
   Size:       1420     254
 
NspI r_CATG'y

Cuts at:      0     273    1674 
   Size:        273    1401
 
PciI A'CATG_T

Cuts at:      0     269    1674 
   Size:        269    1405
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     322    1674 
   Size:        322    1352
 
PfoI T'CCnGG_A

Cuts at:      0     545    1674 
   Size:        545    1129
 
PstI C_TGCA'G

Cuts at:      0    1256    1536    1674 
   Size:       1256     280     138

  Fragments arranged by size: 

                1256     280     138
 
SacI G_AGCT'C

Cuts at:      0      67    1674 
   Size:         67    1607
 
ScaI AGT'ACT

Cuts at:      0     982    1674 
   Size:        982     692
 
SexAI A'CCwGG_T

Cuts at:      0    1523    1674 
   Size:       1523     151
 
SfcI C'TryA_G

Cuts at:      0     748    1252    1532    1674 
   Size:        748     504     280     142

  Fragments arranged by size: 

                 748     504     280     142
 
SmlI C'TyrA_G

Cuts at:      0     718    1180    1674 
   Size:        718     462     494

  Fragments arranged by size: 

                 718     494     462
 
StyI C'CwwG_G

Cuts at:      0     904    1088    1674 
   Size:        904     184     586

  Fragments arranged by size: 

                 904     586     184
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1213    1674 
   Size:       1213     461
 
TatI w'GTAC_w

Cuts at:      0     272     980    1555    1571    1674 
   Size:        272     708     575      16     103

  Fragments arranged by size: 

                 708     575     272     103      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     757     789    1674 
   Size:        757      32     885

  Fragments arranged by size: 

                 885     757      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1201    1674 
   Size:       1201     473
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AleI     AloI    AlwNI    ApaLI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BcgI    BciVI
     BclI    BglII Bme1580I     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BsrFI    BsrGI    BstXI    BstYI     BtgI     BtsI    CspCI   DraIII
     EaeI     EarI  EcoICRI  Eco57MI    EcoRI    Hin4I   HincII  HindIII
     MmeI     MscI     MslI     NaeI     NdeI   NgoMIV     NheI     NspI
     PciI    PflMI     PfoI     PstI     SacI     ScaI    SexAI     SfcI
     SmlI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI
     ApaI     AscI     AseI    AsiSI    AvrII     BbeI     BbsI    BbvCI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BplI    BsaAI    BsaBI
    BsaHI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI     DraI     DrdI     EagI     EciI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NarI     NcoI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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