Restriction map for pF1KB9460
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflIII      AgeI        AhdI        Alw44I      
ApaLI       ApoI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvrII       
BbrPI       BbvCI       BcgI        BciVI       BlnI        
BsaBI       BsaWI       BseAI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
DraI        Eam1105I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        HincII      HindII      HpaI        
KpnI        MamI        MluI        MroI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SacI        
SalI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflII        1336  CTTAAG               C^TTAAG
AscI          579  GGCGCGCC             GG^CGCGCC
AviII        1709  TGCGCA               TGC^GCA
BalI          947  TGGCCA               TGG^CCA
BamHI        1559  GGATCC               G^GATCC
BbsI         1138  GTCTTC               GAAGAC(2/6)
BclI         1130  TGATCA               T^GATCA
BfrI         1336  CTTAAG               C^TTAAG
BglII        1466  AGATCT               A^GATCT
BlpI         1905  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1905  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1138  GTCTTC               GAAGAC(2/6)
BsaAI        1867  TACGTG               [CT]AC^GT[GA]
BsaI         1721  GAGACC               GGTCTC(1/5)
BsrDI        1593  CATTGC               GCAATG(2/0)
CelII        1905  GCTGAGC              GC^T[ACGT]AGC
CpoI          725  CGGTCCG              CG^G[AT]CCG
DraIII       2140  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1291  GACCAGATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       920  CCAAGG               C^C[AT][AT]GG
FbaI         1130  TGATCA               T^GATCA
FspI         1709  TGCGCA               TGC^GCA
HindIII       388  AAGCTT               A^AGCTT
MfeI         1439  CAATTG               C^AATTG
MluNI         947  TGGCCA               TGG^CCA
MscI          947  TGGCCA               TGG^CCA
MunI         1439  CAATTG               C^AATTG
PflMI         678  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII         725  CGGTCCG              CG^G[AT]CCG
SbfI          972  CCTGCAGG             CCTGCA^GG
SfiI          608  GGCCCGTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         2154  CCCGGG               CCC^GGG
Sse8387I      972  CCTGCAGG             CCTGCA^GG
StyI          920  CCAAGG               C^C[AT][AT]GG
TaqI         1667  TCGA                 T^CGA
TfiI          427  GAATC                G^A[AT]TC
Tsp509I      1440  AATT                 ^AATT
TthHB8I      1667  TCGA                 T^CGA
Van91I        678  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        2154  CCCGGG               C^CCGGG
XmaI         2154  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI           10  CCTGAGG              CC^T[ACGT]AGG
             1104  CCTCAGG             
BfaI         1057  CTAG                 C^TAG
             1073  CTAG                
BsiEI          55  CGGCCG               CG[GA][CT]^CG
              572  CGGCCG              
BstAPI       1629  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2139  GCACCCAGTGC         
BstXI         938  CCACAGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1143  CCACCGAGATGG        
Bsu36I         10  CCTGAGG              CC^T[ACGT]AGG
             1104  CCTCAGG             
EagI           55  CGGCCG               C^GGCCG
              572  CGGCCG              
EclXI          55  CGGCCG               C^GGCCG
              572  CGGCCG              
Eco52I         55  CGGCCG               C^GGCCG
              572  CGGCCG              
Eco81I         10  CCTGAGG              CC^T[ACGT]AGG
             1104  CCTCAGG             
EcoNI         313  CCTGAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              591  CCTGCGGGAGG         
KspI          602  CCGCGG               CCGC^GG
              722  CCGCGG              
MaeI         1057  CTAG                 C^TAG
             1073  CTAG                
MslI          216  CACCTATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1144  CACCGAGATG          
NaeI          618  GCCGGC               GCC^GGC
              930  GCCGGC              
NgoMIV        618  GCCGGC               G^CCGGC
              930  GCCGGC              
PleI         1488  GACTC                GAGTC(4/5)
             1222  GAGTC               
PpuMI          62  AGGACCT              [GA]G^G[AT]CC[CT]
              717  GGGTCCC             
PstI          973  CTGCAG               CTGCA^G
             1420  CTGCAG              
PvuII        1633  CAGCTG               CAG^CTG
             1705  CAGCTG              
SacII         602  CCGCGG               CCGC^GG
              722  CCGCGG              
SapI          877  GAAGAGC              GCTCTTC(1/4)
             1474  GAAGAGC             
SfcI          973  CTGCAG               C^T[GA][CT]AG
             1420  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        AvaI        
AvaII       BanI        BanII       BbeI        BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrFI       BsrI        BssHII      BssKI       BssSI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco47III    
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfoI        SmlI        StuI        Tru9I       TseI        
Tsp45I      TspRI       XhoII       
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