Restriction map for pF1KB9454
 (Linear) (Six-base) MAPSORT of: KIBB9454.seq  Check: 4749  from: 1  to: 1557



KIBB9454.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AccI GT'mk_AC

Cuts at:      0      50    1557 
   Size:         50    1507
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     797    1557 
   Size:         91     706     760

  Fragments arranged by size: 

                 760     706      91
 
AflIII A'CryG_T

Cuts at:      0    1069    1557 
   Size:       1069     488
 
AleI CACnn'nnGTG

Cuts at:      0    1024    1557 
   Size:       1024     533
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1324    1401    1557 
   Size:       1324      77     156

  Fragments arranged by size: 

                1324     156      77
 
ApoI r'AATT_y

Cuts at:      0     450    1557 
   Size:        450    1107
 
BanII G_rGCy'C

Cuts at:      0     911    1362    1557 
   Size:        911     451     195

  Fragments arranged by size: 

                 911     451     195
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     110    1066    1557 
   Size:        110     956     491

  Fragments arranged by size: 

                 956     491     110
 
BbvCI CC'TCA_GC

Cuts at:      0     785    1557 
   Size:        785     772
 
BglII A'GATC_T

Cuts at:      0    1305    1470    1557 
   Size:       1305     165      87

  Fragments arranged by size: 

                1305     165      87
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     721     955    1557 
   Size:        721     234     602

  Fragments arranged by size: 

                 721     602     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     776    1557 
   Size:        614     162     781

  Fragments arranged by size: 

                 781     614     162
 
Bpu10I CC'TnA_GC

Cuts at:      0     652     785    1557 
   Size:        652     133     772

  Fragments arranged by size: 

                 772     652     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1088    1125    1557 
   Size:       1088      37     432

  Fragments arranged by size: 

                1088     432      37
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     821    1557 
   Size:        821     736
 
BsaAI yAC'GTr

Cuts at:      0    1230    1557 
   Size:       1230     327
 
BsaWI w'CCGG_w

Cuts at:      0       6    1557 
   Size:          6    1551
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     587     617     749     779     818     848    1557 
   Size:        587      30     132      30      39      30     709

  Fragments arranged by size: 

                 709     587     132      39      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     336     354     712    1557 
   Size:        336      18     358     845

  Fragments arranged by size: 

                 845     358     336      18
 
BseYI C'CCAG_C

Cuts at:      0    1494    1557 
   Size:       1494      63
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     686    1557 
   Size:        686     871
 
BsiHKAI G_wGCw'C

Cuts at:      0     770    1362    1557 
   Size:        770     592     195

  Fragments arranged by size: 

                 770     592     195
 
BsmI GAATG_Cn'

Cuts at:      0    1003    1557 
   Size:       1003     554
 
Bsp1286I G_dGCh'C

Cuts at:      0     770     911    1362    1557 
   Size:        770     141     451     195

  Fragments arranged by size: 

                 770     451     195     141
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     658    1557 
   Size:        658     899
 
BsrGI T'GTAC_A

Cuts at:      0     507    1557 
   Size:        507    1050
 
BstYI r'GATC_y

Cuts at:      0    1305    1470    1557 
   Size:       1305     165      87

  Fragments arranged by size: 

                1305     165      87
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     882    1557 
   Size:        882     675
 
BtsI GCAGTG_nn'

Cuts at:      0    1509    1557 
   Size:       1509      48
 
DraI TTT'AAA

Cuts at:      0      90    1557 
   Size:         90    1467
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1462    1557 
   Size:       1462      95
 
EarI CTCTTCn'nnn_

Cuts at:      0     336     354     363     372     381     387     405     583 
   Size:        336      18       9       9       9       6      18     178

Cuts at:    583    1557 
   Size:        974

  Fragments arranged by size: 

                 974     336     178      18      18       9       9       9
                   6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     213    1557 
   Size:        213    1344
 
EcoICRI GAG'CTC

Cuts at:      0    1360    1557 
   Size:       1360     197
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     614     776     797    1557 
   Size:         91     523     162      21     760

  Fragments arranged by size: 

                 760     523     162      91      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     116     148     313     345     818     850    1557 
   Size:        116      32     165      32     473      32     707

  Fragments arranged by size: 

                 707     473     165     116      32      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      98    1024    1557 
   Size:         98     926     533

  Fragments arranged by size: 

                 926     533      98
 
NspI r_CATG'y

Cuts at:      0    1073    1557 
   Size:       1073     484
 
PasI CC'CwG_GG

Cuts at:      0    1530    1557 
   Size:       1530      27
 
PciI A'CATG_T

Cuts at:      0    1069    1557 
   Size:       1069     488
 
PfoI T'CCnGG_A

Cuts at:      0     591    1557 
   Size:        591     966
 
PshAI GACnn'nnGTC

Cuts at:      0      46    1330    1557 
   Size:         46    1284     227

  Fragments arranged by size: 

                1284     227      46
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     410     442    1557 
   Size:        410      32    1115

  Fragments arranged by size: 

                1115     410      32
 
SacI G_AGCT'C

Cuts at:      0    1362    1557 
   Size:       1362     195
 
SfcI C'TryA_G

Cuts at:      0     844    1038    1557 
   Size:        844     194     519

  Fragments arranged by size: 

                 844     519     194
 
SmlI C'TyrA_G

Cuts at:      0    1103    1140    1557 
   Size:       1103      37     417

  Fragments arranged by size: 

                1103     417      37
 
SnaBI TAC'GTA

Cuts at:      0    1230    1557 
   Size:       1230     327
 
StuI AGG'CCT

Cuts at:      0     815    1557 
   Size:        815     742
 
StyI C'CwwG_G

Cuts at:      0     279    1557 
   Size:        279    1278
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      22      73     853    1220    1486    1557 
   Size:         22      51     780     367     266      71

  Fragments arranged by size: 

                 780     367     266      71      51      22
 
TatI w'GTAC_w

Cuts at:      0     507    1506    1557 
   Size:        507     999      51

  Fragments arranged by size: 

                 999     507      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     648     680     749     781    1557 
   Size:        648      32      69      32     776

  Fragments arranged by size: 

                 776     648      69      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     832    1557 
   Size:        832     725
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1527    1557 
   Size:       1527      30
 
XmnI GAAnn'nnTTC

Cuts at:      0     548    1557 
   Size:        548    1009
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI     ApoI    BanII     BbsI
    BbvCI    BglII     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrGI    BstYI    BtgZI     BtsI     DraI     DrdI     EarI
     EciI  EcoICRI  Eco57MI    Hin4I     MslI     NspI     PasI     PciI
     PfoI    PshAI     PsrI     SacI     SfcI     SmlI    SnaBI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI    BsaBI
    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI   DraIII     EaeI     EagI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI     SspI
     SwaI     XbaI     XhoI     XmaI     ZraI
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