################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MaeIII MamI MfeI MluNI MroI MscI MslI MunI NdeI NheI NotI NruI NsiI PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SfcI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tsp509I Van91I XbaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 886 GTCTAC GT^[AC][GT]AC AflIII 1007 ACGCGT A^C[GA][CT]GT AlwI 315 GATCC GGATC(4/5) AlwNI 686 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 189 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 54 GAGCTC G[AT]GC[AT]^C AspI 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1167 GGACC G^G[AT]CC BbsI 1081 GAAGAC GAAGAC(2/6) BglII 263 AGATCT A^GATCT BpmI 420 CTCCAG CTGGAG(16/14) BpuAI 1081 GAAGAC GAAGAC(2/6) BsiHKAI 54 GAGCTC G[AT]GC[AT]^C Bsp1407I 776 TGTACA T^GTACA BspMI 1088 ACCTGC ACCTGC(4/8) BsrGI 776 TGTACA T^GTACA BsrI 1259 ACTGG ACTGG(1/-1) BstAPI 685 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1078 TTCGAA TT^CGAA BstF5I 654 CATCC GGATG(2/0) BstXI 269 CCAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 1166 GGGACCT [GA]G^G[ACGT]CC[CT] EarI 1038 CTCTTC CTCTTC(1/4) EcoO109I 1166 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 495 CCATGG C^C[AT][AT]GG FokI 654 CATCC GGATG(9/13) Ksp632I 1038 CTCTTC CTCTTC(1/4) KspI 951 CCGCGG CCGC^GG MaeII 910 ACGT A^CGT MluI 1007 ACGCGT A^CGCGT MseI 1178 TTAA T^TAA NcoI 495 CCATGG C^CATGG NspV 1078 TTCGAA TT^CGAA PflFI 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1166 GGGACCT [GA]G^G[AT]CC[CT] SacI 54 GAGCTC GAGCT^C SacII 951 CCGCGG CCGC^GG SexAI 1283 ACCTGGT A^CC[AT]GGT SfaNI 1274 GCATC GCATC(5/9) SfuI 1078 TTCGAA TT^CGAA SmaI 849 CCCGGG CCC^GGG SspBI 776 TGTACA T^GTACA StyI 495 CCATGG C^C[AT][AT]GG TfiI 86 GAATC G^A[AT]TC Tru9I 1178 TTAA T^TAA TspRI 480 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 647 CCAAGCTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 849 CCCGGG C^CCGGG XmaI 849 CCCGGG C^CCGGG XmnI 189 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 381 GTAC GT^AC 777 GTAC Aor51HI 36 AGCGCT AGC^GCT 837 AGCGCT BsgI 1224 CTGCAC GTGCAG(16/14) 4 GTGCAG BsmAI 464 GAGAC GTCTC(1/5) 1256 GAGAC BssHII 969 GCGCGC G^CGCGC 995 GCGCGC BstYI 263 AGATCT [GA]^GATC[CT] 314 AGATCC EagI 431 CGGCCG C^GGCCG 930 CGGCCG EclXI 431 CGGCCG C^GGCCG 930 CGGCCG Eco47III 36 AGCGCT AGC^GCT 837 AGCGCT Eco52I 431 CGGCCG C^GGCCG 930 CGGCCG HgaI 884 GCGTC GACGC(5/10) 1009 GCGTC MflI 263 AGATCT [GA]^GATC[CT] 314 AGATCC MspA1I 686 CAGCGG C[AC]G^C[GT]G 951 CCGCGG NspI 248 ACATGC [GA]CATG^[CT] 1124 GCATGT PaeR7I 76 CTCGAG C^TCGAG 1264 CTCGAG RsaI 381 GTAC GT^AC 777 GTAC XhoI 76 CTCGAG C^TCGAG 1264 CTCGAG XhoII 263 AGATCT [GA]^GATC[CT] 314 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AcyI AluI AvaI BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MboI MboII MnlI MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfoI SmlI TaqI TseI TthHB8I