Restriction map for pF1KB9426
 (Linear) (Six-base) MAPSORT of: KIBB9426.seq  Check: 8061  from: 1  to: 2049



KIBB9426.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AccI GT'mk_AC

Cuts at:      0    1604    1715    2049 
   Size:       1604     111     334

  Fragments arranged by size: 

                1604     334     111
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     443     805    1177    2049 
   Size:        443     362     372     872

  Fragments arranged by size: 

                 872     443     372     362
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     321    2049 
   Size:        120     201    1728

  Fragments arranged by size: 

                1728     201     120
 
ApaI G_GGCC'C

Cuts at:      0    1860    2049 
   Size:       1860     189
 
ApaLI G'TGCA_C

Cuts at:      0     615    2049 
   Size:        615    1434
 
AvaI C'yCGr_G

Cuts at:      0     244     370    1910    2049 
   Size:        244     126    1540     139

  Fragments arranged by size: 

                1540     244     139     126
 
BamHI G'GATC_C

Cuts at:      0    1214    1748    2049 
   Size:       1214     534     301

  Fragments arranged by size: 

                1214     534     301
 
BanI G'GyrC_C

Cuts at:      0      10     337    2049 
   Size:         10     327    1712

  Fragments arranged by size: 

                1712     327      10
 
BanII G_rGCy'C

Cuts at:      0    1368    1860    2049 
   Size:       1368     492     189

  Fragments arranged by size: 

                1368     492     189
 
BbeI G_GCGC'C

Cuts at:      0     341    2049 
   Size:        341    1708
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     190     249    2049 
   Size:        190      59    1800

  Fragments arranged by size: 

                1800     190      59
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     606     640    2049 
   Size:        606      34    1409

  Fragments arranged by size: 

                1409     606      34
 
BglII A'GATC_T

Cuts at:      0    1434    2049 
   Size:       1434     615
 
Bme1580I G_kGCm'C

Cuts at:      0     619    1860    2049 
   Size:        619    1241     189

  Fragments arranged by size: 

                1241     619     189
 
BmgBI CAC'GTC

Cuts at:      0    1167    2049 
   Size:       1167     882
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1863    2049 
   Size:       1863     186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418    1550    1561    2018    2049 
   Size:        418    1132      11     457      31

  Fragments arranged by size: 

                1132     457     418      31      11
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1845    2049 
   Size:       1845     204
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     434     934    1127    2049 
   Size:        434     500     193     922

  Fragments arranged by size: 

                 922     500     434     193
 
BsaAI yAC'GTr

Cuts at:      0     200     530    2049 
   Size:        200     330    1519

  Fragments arranged by size: 

                1519     330     200
 
BsaBI GATnn'nnATC

Cuts at:      0     727    2049 
   Size:        727    1322
 
BsaHI Gr'CG_yC

Cuts at:      0     268     338    2049 
   Size:        268      70    1711

  Fragments arranged by size: 

                1711     268      70
 
BsaWI w'CCGG_w

Cuts at:      0     443    2049 
   Size:        443    1606
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     223    2049 
   Size:        223    1826
 
BseYI C'CCAG_C

Cuts at:      0    1859    2049 
   Size:       1859     190
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285    2049 
   Size:        285    1764
 
BsiEI CG_ry'CG

Cuts at:      0     335    1281    2049 
   Size:        335     946     768

  Fragments arranged by size: 

                 946     768     335
 
BsiHKAI G_wGCw'C

Cuts at:      0     619    1368    1817    2049 
   Size:        619     749     449     232

  Fragments arranged by size: 

                 749     619     449     232
 
BsmI GAATG_Cn'

Cuts at:      0    1198    2049 
   Size:       1198     851
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     259    2049 
   Size:        259    1790
 
Bsp1286I G_dGCh'C

Cuts at:      0     619    1368    1817    1860    2049 
   Size:        619     749     449      43     189

  Fragments arranged by size: 

                 749     619     449     189      43
 
BspHI T'CATG_A

Cuts at:      0    1763    2049 
   Size:       1763     286
 
BsrDI GCAATG_nn'

Cuts at:      0    1524    1622    2049 
   Size:       1524      98     427

  Fragments arranged by size: 

                1524     427      98
 
BsrFI r'CCGG_y

Cuts at:      0     334    2049 
   Size:        334    1715
 
BssSI C'ACGA_G

Cuts at:      0     262    1361    1465    2049 
   Size:        262    1099     104     584

  Fragments arranged by size: 

                1099     584     262     104
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     508     915    1788    2049 
   Size:        508     407     873     261

  Fragments arranged by size: 

                 873     508     407     261
 
BstYI r'GATC_y

Cuts at:      0     366     446    1214    1434    1571    1748    2049 
   Size:        366      80     768     220     137     177     301

  Fragments arranged by size: 

                 768     366     301     220     177     137      80
 
BtgI C'CryG_G

Cuts at:      0    1529    2049 
   Size:       1529     520
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1855    2049 
   Size:       1855     194
 
BtsI GCAGTG_nn'

Cuts at:      0      62     558     790    2049 
   Size:         62     496     232    1259

  Fragments arranged by size: 

                1259     496     232      62
 
DraIII CAC_nnn'GTG

Cuts at:      0     291    2049 
   Size:        291    1758
 
EaeI y'GGCC_r

Cuts at:      0     237     251     293     524    1532    2049 
   Size:        237      14      42     231    1008     517

  Fragments arranged by size: 

                1008     517     237     231      42      14
 
EarI CTCTTCn'nnn_

Cuts at:      0     103    1946    2049 
   Size:        103    1843     103

  Fragments arranged by size: 

                1843     103     103
 
EcoICRI GAG'CTC

Cuts at:      0    1366    2049 
   Size:       1366     683
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     443     805    1177    1550    1561    2018    2049 
   Size:        418      25     362     372     373      11     457      31

  Fragments arranged by size: 

                 457     418     373     372     362      31      25      11
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1687    1801    2049 
   Size:       1687     114     248

  Fragments arranged by size: 

                1687     248     114
 
FspI TGC'GCA

Cuts at:      0     328    2049 
   Size:        328    1721
 
HaeII r_GCGC'y

Cuts at:      0     341     474    2049 
   Size:        341     133    1575

  Fragments arranged by size: 

                1575     341     133
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     431     463    1037    1069    1206    1238    1263    1295 
   Size:        431      32     574      32     137      32      25      32

Cuts at:   1295    1886    1904    1918    1936    2049 
   Size:        591      18      14      18     113

  Fragments arranged by size: 

                 591     574     431     137     113      32      32      32
                  32      25      18      18      14
 
KasI G'GCGC_C

Cuts at:      0     337    2049 
   Size:        337    1712
 
MfeI C'AATT_G

Cuts at:      0    1970    2049 
   Size:       1970      79
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     957     976    1064    1439    2049 
   Size:        957      19      88     375     610

  Fragments arranged by size: 

                 957     610     375      88      19
 
MscI TGG'CCA

Cuts at:      0     295     526    2049 
   Size:        295     231    1523

  Fragments arranged by size: 

                1523     295     231
 
MslI CAynn'nnrTG

Cuts at:      0    1075    1736    2049 
   Size:       1075     661     313

  Fragments arranged by size: 

                1075     661     313
 
MspA1I CmG'CkG

Cuts at:      0      77     117    1863    2049 
   Size:         77      40    1746     186

  Fragments arranged by size: 

                1746     186      77      40
 
NarI GG'CG_CC

Cuts at:      0     338    2049 
   Size:        338    1711
 
PasI CC'CwG_GG

Cuts at:      0     273    2049 
   Size:        273    1776
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      77     120     291    2049 
   Size:         77      43     171    1758

  Fragments arranged by size: 

                1758     171      77      43
 
PfoI T'CCnGG_A

Cuts at:      0     314    1577    2049 
   Size:        314    1263     472

  Fragments arranged by size: 

                1263     472     314
 
PspOMI G'GGCC_C

Cuts at:      0    1856    2049 
   Size:       1856     193
 
PspXI vC'TCGA_Gb

Cuts at:      0     244    2049 
   Size:        244    1805
 
PstI C_TGCA'G

Cuts at:      0      68     311     565    1178    1383    1602    2049 
   Size:         68     243     254     613     205     219     447

  Fragments arranged by size: 

                 613     447     254     243     219     205      68
 
PvuI CG_AT'CG

Cuts at:      0    1281    2049 
   Size:       1281     768
 
PvuII CAG'CTG

Cuts at:      0     117    1863    2049 
   Size:        117    1746     186

  Fragments arranged by size: 

                1746     186     117
 
SacI G_AGCT'C

Cuts at:      0    1368    2049 
   Size:       1368     681
 
SapI GCTCTTCn'nnn_

Cuts at:      0     103    1946    2049 
   Size:        103    1843     103

  Fragments arranged by size: 

                1843     103     103
 
SexAI A'CCwGG_T

Cuts at:      0     149    2049 
   Size:        149    1900
 
SfcI C'TryA_G

Cuts at:      0      64     307     561    1174    1379    1428    1598    2049 
   Size:         64     243     254     613     205      49     170     451

  Fragments arranged by size: 

                 613     451     254     243     205     170      64      49
            
 
SfoI GGC'GCC

Cuts at:      0     339    2049 
   Size:        339    1710
 
SmlI C'TyrA_G

Cuts at:      0     244     370    1824    2049 
   Size:        244     126    1454     225

  Fragments arranged by size: 

                1454     244     225     126
 
SspI AAT'ATT

Cuts at:      0     681     945    2049 
   Size:        681     264    1104

  Fragments arranged by size: 

                1104     681     264
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     351    1219    2049 
   Size:        351     868     830

  Fragments arranged by size: 

                 868     830     351
 
TatI w'GTAC_w

Cuts at:      0     664    2049 
   Size:        664    1385
 
Tth111I GACn'n_nGTC

Cuts at:      0    1450    2049 
   Size:       1450     599
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     505    1785    2049 
   Size:        505    1280     264

  Fragments arranged by size: 

                1280     505     264
 
XhoI C'TCGA_G

Cuts at:      0     244     370    2049 
   Size:        244     126    1679

  Fragments arranged by size: 

                1679     244     126
 
XmnI GAAnn'nnTTC

Cuts at:      0     945    2019    2049 
   Size:        945    1074      30

  Fragments arranged by size: 

                1074     945      30
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BglII Bme1580I    BmgBI     BmrI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BsrDI    BsrFI    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI     FspI    HaeII
    Hin4I     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NarI
     PasI    PflMI     PfoI   PspOMI    PspXI     PstI     PvuI    PvuII
     SacI     SapI    SexAI     SfcI     SfoI     SmlI     SspI    TaqII
     TatI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI
     BmtI     BplI   Bpu10I    BsaXI    BsiWI    BspEI    BspMI    BsrBI
    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI     EagI     EciI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MluI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI     PsrI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI     TstI     XbaI     XmaI     ZraI
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