Restriction map for pF1KB9422
 (Linear) (Six-base) MAPSORT of: KIBB9422.seq  Check: 8350  from: 1  to: 1713



KIBB9422.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572    1270    1713 
   Size:        572     698     443

  Fragments arranged by size: 

                 698     572     443
 
AflIII A'CryG_T

Cuts at:      0      98    1679    1713 
   Size:         98    1581      34

  Fragments arranged by size: 

                1581      98      34
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1349    1713 
   Size:       1349     364
 
AlwNI CAG_nnn'CTG

Cuts at:      0     690    1610    1713 
   Size:        690     920     103

  Fragments arranged by size: 

                 920     690     103
 
ApaI G_GGCC'C

Cuts at:      0     602    1713 
   Size:        602    1111
 
ApoI r'AATT_y

Cuts at:      0    1002    1433    1713 
   Size:       1002     431     280

  Fragments arranged by size: 

                1002     431     280
 
BanII G_rGCy'C

Cuts at:      0     140     602    1380    1713 
   Size:        140     462     778     333

  Fragments arranged by size: 

                 778     462     333     140
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1508    1713 
   Size:       1508     205
 
BbvCI CC'TCA_GC

Cuts at:      0    1598    1713 
   Size:       1598     115
 
BfrBI ATG'CAT

Cuts at:      0    1189    1713 
   Size:       1189     524
 
BglII A'GATC_T

Cuts at:      0     631    1713 
   Size:        631    1082
 
BlpI GC'TnA_GC

Cuts at:      0     950    1713 
   Size:        950     763
 
Bme1580I G_kGCm'C

Cuts at:      0     602    1713 
   Size:        602    1111
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      41    1713 
   Size:         41    1672
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369     492     877    1713 
   Size:        369     123     385     836

  Fragments arranged by size: 

                 836     385     369     123
 
Bpu10I CC'TnA_GC

Cuts at:      0     427    1598    1713 
   Size:        427    1171     115

  Fragments arranged by size: 

                1171     427     115
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318     481     727    1713 
   Size:        318     163     246     986

  Fragments arranged by size: 

                 986     318     246     163
 
BsaAI yAC'GTr

Cuts at:      0    1695    1713 
   Size:       1695      18
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     875     905    1713 
   Size:        875      30     808

  Fragments arranged by size: 

                 875     808      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     223     507     682    1607    1713 
   Size:        127      96     284     175     925     106

  Fragments arranged by size: 

                 925     284     175     127     106      96
 
BsiHKAI G_wGCw'C

Cuts at:      0     140     826    1713 
   Size:        140     686     887

  Fragments arranged by size: 

                 887     686     140
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     602     826    1380    1713 
   Size:        140     462     224     554     333

  Fragments arranged by size: 

                 554     462     333     224     140
 
BspHI T'CATG_A

Cuts at:      0     303    1437    1713 
   Size:        303    1134     276

  Fragments arranged by size: 

                1134     303     276
 
BsrDI GCAATG_nn'

Cuts at:      0    1331    1459    1713 
   Size:       1331     128     254

  Fragments arranged by size: 

                1331     254     128
 
BssSI C'ACGA_G

Cuts at:      0     603     624    1713 
   Size:        603      21    1089

  Fragments arranged by size: 

                1089     603      21
 
BstBI TT'CG_AA

Cuts at:      0     659    1499    1713 
   Size:        659     840     214

  Fragments arranged by size: 

                 840     659     214
 
BstYI r'GATC_y

Cuts at:      0     631    1713 
   Size:        631    1082
 
Bsu36I CC'TnA_GG

Cuts at:      0     700    1372    1713 
   Size:        700     672     341

  Fragments arranged by size: 

                 700     672     341
 
BtsI GCAGTG_nn'

Cuts at:      0    1250    1713 
   Size:       1250     463
 
EarI CTCTTCn'nnn_

Cuts at:      0     320     839     958    1340    1713 
   Size:        320     519     119     382     373

  Fragments arranged by size: 

                 519     382     373     320     119
 
EcoICRI GAG'CTC

Cuts at:      0     138    1713 
   Size:        138    1575
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369     492     572     877    1270    1713 
   Size:        369     123      80     305     393     443

  Fragments arranged by size: 

                 443     393     369     305     123      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     369     431     987    1350    1713 
   Size:        369      62     556     363     363

  Fragments arranged by size: 

                 556     369     363     363      62
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     672    1713 
   Size:        672    1041
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1114    1146    1521    1553    1713 
   Size:       1114      32     375      32     160

  Fragments arranged by size: 

                1114     375     160      32      32
 
HincII GTy'rAC

Cuts at:      0     674    1713 
   Size:        674    1039
 
HindIII A'AGCT_T

Cuts at:      0    1165    1713 
   Size:       1165     548
 
HpaI GTT'AAC

Cuts at:      0     674    1713 
   Size:        674    1039
 
MfeI C'AATT_G

Cuts at:      0    1199    1713 
   Size:       1199     514
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     846    1713 
   Size:        846     867
 
MslI CAynn'nnrTG

Cuts at:      0     308    1184    1713 
   Size:        308     876     529

  Fragments arranged by size: 

                 876     529     308
 
MspA1I CmG'CkG

Cuts at:      0      20     159     512    1305    1467    1623    1713 
   Size:         20     139     353     793     162     156      90

  Fragments arranged by size: 

                 793     353     162     156     139      90      20
 
NsiI A_TGCA'T

Cuts at:      0    1191    1713 
   Size:       1191     522
 
NspI r_CATG'y

Cuts at:      0    1086    1713 
   Size:       1086     627
 
PasI CC'CwG_GG

Cuts at:      0     395    1713 
   Size:        395    1318
 
PmlI CAC'GTG

Cuts at:      0    1695    1713 
   Size:       1695      18
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     479     511    1713 
   Size:        479      32    1202

  Fragments arranged by size: 

                1202     479      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     369     431    1350    1713 
   Size:        369      62     919     363

  Fragments arranged by size: 

                 919     369     363      62
 
PspOMI G'GGCC_C

Cuts at:      0     598    1713 
   Size:        598    1115
 
PstI C_TGCA'G

Cuts at:      0     125     494     639    1713 
   Size:        125     369     145    1074

  Fragments arranged by size: 

                1074     369     145     125
 
PvuII CAG'CTG

Cuts at:      0      20     159    1305    1467    1713 
   Size:         20     139    1146     162     246

  Fragments arranged by size: 

                1146     246     162     139      20
 
SacI G_AGCT'C

Cuts at:      0     140    1713 
   Size:        140    1573
 
SapI GCTCTTCn'nnn_

Cuts at:      0     320    1713 
   Size:        320    1393
 
SfcI C'TryA_G

Cuts at:      0     121     490     635    1147    1713 
   Size:        121     369     145     512     566

  Fragments arranged by size: 

                 566     512     369     145     121
 
SmlI C'TyrA_G

Cuts at:      0     297     496     742    1713 
   Size:        297     199     246     971

  Fragments arranged by size: 

                 971     297     246     199
 
SphI G_CATG'C

Cuts at:      0    1086    1713 
   Size:       1086     627
 
SspI AAT'ATT

Cuts at:      0    1629    1713 
   Size:       1629      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     101     160    1713 
   Size:        101      59    1553

  Fragments arranged by size: 

                1553     101      59
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     860     875     892     907    1713 
   Size:        860      15      17      15     806

  Fragments arranged by size: 

                 860     806      17      15      15
 
XmnI GAAnn'nnTTC

Cuts at:      0     655    1713 
   Size:        655    1058
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     ApaI     ApoI    BanII     BbsI
    BbvCI    BfrBI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI    BsrDI
    BssSI    BstBI    BstYI   Bsu36I     BtsI     EarI  EcoICRI  Eco57MI
 EcoO109I     FalI    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI
     MslI   MspA1I     NsiI     NspI     PasI     PmlI     PpiI    PpuMI
   PspOMI     PstI    PvuII     SacI     SapI     SfcI     SmlI     SphI
     SspI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
     BglI    BmgBI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI    PflMI
     PfoI     PmeI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     StyI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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