Restriction map for pF1KB9418
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# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
BalI        BanI        BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MluI        MluNI       MscI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccIII       1148  TCCGGA               T^CCGGA
AciI         1471  GCGG                 CCGC(-3/-1)
AflIII        111  ACATGT               A^C[GA][CT]GT
AlwNI        1376  CAGGTACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       848  AGCGCT               AGC^GCT
ApaI         1497  GGGCCC               GGGCC^C
Asp700I       109  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         797  GAGCAC               G[AT]GC[AT]^C
AvrII        1297  CCTAGG               C^CTAGG
BamHI         906  GGATCC               G^GATCC
BbsI          927  GTCTTC               GAAGAC(2/6)
BbvCI         304  GCTGAGG              CCTCAGC(-5/-2)
BcnI          490  CCGGG                CC^[GC]GG
BglII         882  AGATCT               A^GATCT
BlnI         1297  CCTAGG               C^CTAGG
BpmI          652  CTCCAG               CTGGAG(16/14)
BpuAI         927  GTCTTC               GAAGAC(2/6)
BsaBI         901  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          784  GAGACC               GGTCTC(1/5)
BsaWI        1148  TCCGGA               [AT]^CCGG[AT]
BseAI        1148  TCCGGA               T^CCGGA
BsiEI         982  CGGTCG               CG[GA][CT]^CG
BsiHKAI       797  GAGCAC               G[AT]GC[AT]^C
BsmFI        1183  GTCCC                GGGAC(10/14)
BspEI        1148  TCCGGA               T^CCGGA
BspHI         267  TCATGA               T^CATGA
BspLU11I      111  ACATGT               A^CATGT
BstEII       1602  GGTGACC              G^GT[ACGT]ACC
BstPI        1602  GGTGACC              G^GT[ACGT]ACC
CfoI          849  GCGC                 GCG^C
DsaI          788  CCATGG               C^C[GA][CT]GG
Eco47III      848  AGCGCT               AGC^GCT
EcoO65I      1602  GGTGACC              G^GT[ACGT]ACC
EcoRI        1044  GAATTC               G^AATTC
FauI         1471  GCGGG                CCCGC(4/6)
HaeII         848  AGCGCT               [GA]GCGC^[CT]
HhaI          849  GCGC                 GCG^C
HinP1I        849  GCGC                 G^CGC
HindIII       257  AAGCTT               A^AGCTT
MaeII          50  ACGT                 A^CGT
MamI          901  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1148  TCCGGA               T^CCGGA
MslI          411  CACCCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1627  CAGCTG               C[AC]G^C[GT]G
NciI          490  CCGGG                CC^[GC]GG
NcoI          788  CCATGG               C^CATGG
NspI          111  ACATGT               [GA]CATG^[CT]
PflMI         740  CCAGGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1517  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1456  CTGCAG               CTGCA^G
PvuII        1627  CAGCTG               CAG^CTG
RcaI          267  TCATGA               T^CATGA
ScaI         1434  AGTACT               AGT^ACT
SmlI         1676  CTCAAG               C^T[CT][GA]AG
TaqI          985  TCGA                 T^CGA
TthHB8I       985  TCGA                 T^CGA
Van91I        740  CCAGGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1064  CCAAAGATCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          109  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AvaII         689  GGACC                G^G[AT]CC
             1518  GGTCC               
BsgI         1592  CTGCAC               GTGCAG(16/14)
             1111  GTGCAG              
BsrDI        1529  GCAATG               GCAATG(2/0)
             1547  GCAATG              
BsrFI         145  GCCGGT               [GA]^CCGG[CT]
              980  GCCGGT              
Cac8I        1357  GCCAGC               GC[ACGT]^[ACGT]GC
             1471  GCGGGC              
Cfr10I        145  GCCGGT               [GA]^CCGG[CT]
              980  GCCGGT              
DraI          712  TTTAAA               TTT^AAA
              865  TTTAAA              
DraII        1497  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             1517  AGGTCCT             
EcoO109I     1497  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             1517  AGGTCCT             
EcoT14I       788  CCATGG               C^C[AT][AT]GG
             1297  CCTAGG              
HaeIII        334  GGCC                 GG^CC
             1498  GGCC                
MfeI          284  CAATTG               C^AATTG
             1487  CAATTG              
MunI          284  CAATTG               C^AATTG
             1487  CAATTG              
PleI          484  GACTC                GAGTC(4/5)
              925  GAGTC               
SfcI         1025  CTACAG               C^T[GA][CT]AG
             1456  CTGCAG              
StyI          788  CCATGG               C^C[AT][AT]GG
             1297  CCTAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BanII       BbvI        BfaI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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