Restriction map for pF1KB9414
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AgeI        AhdI        AocI        
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbvCI       BciVI       BclI        BcnI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaHI       BsaI        BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
EcoT22I     FbaI        FseI        FspI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          131  GAATTC               [GA]^AATT[CT]
AflII         556  CTTAAG               C^TTAAG
AflIII        309  ACGTGT               A^C[GA][CT]GT
Aor51HI      1942  AGCGCT               AGC^GCT
ApoI          131  GAATTC               [GA]^AATT[CT]
BamHI        1613  GGATCC               G^GATCC
BanII          55  GAGCCC               G[GA]GC[CT]^C
BcgI          126  GCATCGAATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          556  CTTAAG               C^TTAAG
BsaAI         308  TACGTG               [CT]AC^GT[GA]
BsaBI        1882  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         491  TCCGGT               [AT]^CCGG[AT]
BsgI         1996  CTGCAC               GTGCAG(16/14)
BsmAI           3  GTCTC                GTCTC(1/5)
BspHI         639  TCATGA               T^CATGA
BsrBI        1448  GAGCGG               CCGCTC(-3/-3)
DraII        1916  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI          659  CGGCCA               [CT]^GGCC[GA]
Eco47III     1942  AGCGCT               AGC^GCT
EcoNI         973  CCTTTTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1916  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         131  GAATTC               G^AATTC
HapII         492  CCGG                 C^CGG
HincII        526  GTCAAC               GT[CT]^[GA]AC
HindII        526  GTCAAC               GT[CT]^[GA]AC
HpaII         492  CCGG                 C^CGG
MamI         1882  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI         1923  CACAGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          492  CCGG                 C^CGG
NdeI          443  CATATG               CA^TATG
PvuII        1812  CAGCTG               CAG^CTG
RcaI          639  TCATGA               T^CATGA
SapI          217  GAAGAGC              GCTCTTC(1/4)
SexAI         909  ACCTGGT              A^CC[AT]GGT
SmlI          556  CTTAAG               C^T[CT][GA]AG
SspI          389  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Alw44I        962  GTGCAC               G^TGCAC
             1757  GTGCAC              
AlwNI        1083  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1346  CAGGCTCTG           
ApaLI         962  GTGCAC               G^TGCAC
             1757  GTGCAC              
AspHI         962  GTGCAC               G[AT]GC[AT]^C
             1757  GTGCAC              
BseRI        1314  CTCCTC               GAGGAG(10/8)
             2024  CTCCTC              
BsiHKAI       962  GTGCAC               G[AT]GC[AT]^C
             1757  GTGCAC              
BsmFI        1799  GGGAC                GGGAC(10/14)
              969  GTCCC               
BstYI        1613  GGATCC               [GA]^GATC[CT]
             1988  AGATCC              
DraI          375  TTTAAA               TTT^AAA
             1650  TTTAAA              
EcoRV          73  GATATC               GAT^ATC
              879  GATATC              
EcoT14I        59  CCTTGG               C^C[AT][AT]GG
             1495  CCTTGG              
HgaI          502  GACGC                GACGC(5/10)
             1849  GCGTC               
HphI          862  GGTGA                GGTGA(8/7)
              901  TCACC               
MaeII         309  ACGT                 A^CGT
             1765  ACGT                
MflI         1613  GGATCC               [GA]^GATC[CT]
             1988  AGATCC              
MspA1I       1812  CAGCTG               C[AC]G^C[GT]G
             1993  CCGCTG              
NspI          163  GCATGT               [GA]CATG^[CT]
              629  GCATGT              
PleI         1870  GACTC                GAGTC(4/5)
              994  GAGTC               
StyI           59  CCTTGG               C^C[AT][AT]GG
             1495  CCTTGG              
XhoII        1613  GGATCC               [GA]^GATC[CT]
             1988  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbsI        BbvI        BfaI        BmyI        BpuAI       
BsaJI       BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HhaI        HinP1I      HinfI       ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PflMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      
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