Restriction map for pF1KB9414
 (Linear) (Six-base) MAPSORT of: KIBB9414.seq  Check: 57  from: 1  to: 2139



KIBB9414.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739     821    1291    1838    1897    1962    2064    2139 
   Size:        739      82     470     547      59      65     102      75

  Fragments arranged by size: 

                 739     547     470     102      82      75      65      59
            
 
AfeI AGC'GCT

Cuts at:      0    1944    2139 
   Size:       1944     195
 
AflII C'TTAA_G

Cuts at:      0     556    2139 
   Size:        556    1583
 
AflIII A'CryG_T

Cuts at:      0     309    2139 
   Size:        309    1830
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1385    2139 
   Size:       1385     754
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1088    1351    2139 
   Size:       1088     263     788

  Fragments arranged by size: 

                1088     788     263
 
ApaLI G'TGCA_C

Cuts at:      0     962    1757    2139 
   Size:        962     795     382

  Fragments arranged by size: 

                 962     795     382
 
ApoI r'AATT_y

Cuts at:      0     131    2139 
   Size:        131    2008
 
BamHI G'GATC_C

Cuts at:      0    1613    2139 
   Size:       1613     526
 
BanII G_rGCy'C

Cuts at:      0      59    2139 
   Size:         59    2080
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      32     191     506    1844    2139 
   Size:         32     159     315    1338     295

  Fragments arranged by size: 

                1338     315     295     159      32
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     115     149    2139 
   Size:        115      34    1990

  Fragments arranged by size: 

                1990     115      34
 
Bme1580I G_kGCm'C

Cuts at:      0      43     966    1686    1761    2139 
   Size:         43     923     720      75     378

  Fragments arranged by size: 

                 923     720     378      75      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1132    2139 
   Size:       1132    1007
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1445    2139 
   Size:       1445     694
 
Bpu10I CC'TnA_GC

Cuts at:      0    1242    1678    2139 
   Size:       1242     436     461

  Fragments arranged by size: 

                1242     461     436
 
BsaAI yAC'GTr

Cuts at:      0     310    2139 
   Size:        310    1829
 
BsaBI GATnn'nnATC

Cuts at:      0    1886    2139 
   Size:       1886     253
 
BsaWI w'CCGG_w

Cuts at:      0     491    2139 
   Size:        491    1648
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1305    2015    2139 
   Size:       1305     710     124

  Fragments arranged by size: 

                1305     710     124
 
BseYI C'CCAG_C

Cuts at:      0     189     872    1063    1824    1939    2089    2139 
   Size:        189     683     191     761     115     150      50

  Fragments arranged by size: 

                 761     683     191     189     150     115      50
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1981    2139 
   Size:       1981     158
 
BsiHKAI G_wGCw'C

Cuts at:      0     966    1761    2139 
   Size:        966     795     378

  Fragments arranged by size: 

                 966     795     378
 
Bsp1286I G_dGCh'C

Cuts at:      0      43      59     966    1686    1761    2139 
   Size:         43      16     907     720      75     378

  Fragments arranged by size: 

                 907     720     378      75      43      16
 
BspHI T'CATG_A

Cuts at:      0     639    2139 
   Size:        639    1500
 
BsrBI CCG'CTC

Cuts at:      0    1450    2139 
   Size:       1450     689
 
BstYI r'GATC_y

Cuts at:      0    1613    1988    2139 
   Size:       1613     375     151

  Fragments arranged by size: 

                1613     375     151
 
BtsI GCAGTG_nn'

Cuts at:      0     616     802    1772    2139 
   Size:        616     186     970     367

  Fragments arranged by size: 

                 970     616     367     186
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     896     931    1695    1730    2139 
   Size:        896      35     764      35     409

  Fragments arranged by size: 

                 896     764     409      35      35
 
DraI TTT'AAA

Cuts at:      0     377    1652    2139 
   Size:        377    1275     487

  Fragments arranged by size: 

                1275     487     377
 
EaeI y'GGCC_r

Cuts at:      0     659    2139 
   Size:        659    1480
 
EarI CTCTTCn'nnn_

Cuts at:      0     212    1123    1310    1323    1880    2139 
   Size:        212     911     187      13     557     259

  Fragments arranged by size: 

                 911     557     259     212     187      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739     821    1291    1838    1897    1962    2064    2139 
   Size:        739      82     470     547      59      65     102      75

  Fragments arranged by size: 

                 739     547     470     102      82      75      65      59
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     977    2139 
   Size:        977    1162
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1917    2139 
   Size:       1917     222
 
EcoRI G'AATT_C

Cuts at:      0     131    2139 
   Size:        131    2008
 
EcoRV GAT'ATC

Cuts at:      0      75     881    2139 
   Size:         75     806    1258

  Fragments arranged by size: 

                1258     806      75
 
HaeII r_GCGC'y

Cuts at:      0     357    1946    1973    2139 
   Size:        357    1589      27     166

  Fragments arranged by size: 

                1589     357     166      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     985    1017    2139 
   Size:        985      32    1122

  Fragments arranged by size: 

                1122     985      32
 
HincII GTy'rAC

Cuts at:      0     528    2139 
   Size:        528    1611
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1766    1793    1990    2139 
   Size:       1766      27     197     149

  Fragments arranged by size: 

                1766     197     149      27
 
MslI CAynn'nnrTG

Cuts at:      0    1927    2139 
   Size:       1927     212
 
MspA1I CmG'CkG

Cuts at:      0    1814    1995    2139 
   Size:       1814     181     144

  Fragments arranged by size: 

                1814     181     144
 
NdeI CA'TA_TG

Cuts at:      0     444    2139 
   Size:        444    1695
 
NspI r_CATG'y

Cuts at:      0     167     633    2139 
   Size:        167     466    1506

  Fragments arranged by size: 

                1506     466     167
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     910    1088    1351    2139 
   Size:        910     178     263     788

  Fragments arranged by size: 

                 910     788     263     178
 
PsiI TTA'TAA

Cuts at:      0     200    1457    2139 
   Size:        200    1257     682

  Fragments arranged by size: 

                1257     682     200
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     701     733    2139 
   Size:        701      32    1406

  Fragments arranged by size: 

                1406     701      32
 
PstI C_TGCA'G

Cuts at:      0     589     809    1374    2139 
   Size:        589     220     565     765

  Fragments arranged by size: 

                 765     589     565     220
 
PvuII CAG'CTG

Cuts at:      0    1814    2139 
   Size:       1814     325
 
SapI GCTCTTCn'nnn_

Cuts at:      0     212    2139 
   Size:        212    1927
 
SexAI A'CCwGG_T

Cuts at:      0     909    2139 
   Size:        909    1230
 
SfcI C'TryA_G

Cuts at:      0     585     805    1094    1151    1370    1619    1640    2113 
   Size:        585     220     289      57     219     249      21     473

Cuts at:   2113    2139 
   Size:         26

  Fragments arranged by size: 

                 585     473     289     249     220     219      57      26
                  21
 
SmlI C'TyrA_G

Cuts at:      0     556    2139 
   Size:        556    1583
 
SspI AAT'ATT

Cuts at:      0     391    2139 
   Size:        391    1748
 
StyI C'CwwG_G

Cuts at:      0      59    1495    2139 
   Size:         59    1436     644

  Fragments arranged by size: 

                1436     644      59
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1827    1859    2139 
   Size:       1827      32     280

  Fragments arranged by size: 

                1827     280      32
 

 Enzymes that do cut:

     AcuI     AfeI    AflII   AflIII     AlfI    AlwNI    ApaLI     ApoI
    BamHI    BanII     BbsI     BcgI Bme1580I     BmrI     BplI   Bpu10I
    BsaAI    BsaBI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BsrBI    BstYI     BtsI    CspCI     DraI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII
     MmeI     MslI   MspA1I     NdeI     NspI    PflMI     PsiI     PsrI
     PstI    PvuII     SapI    SexAI     SfcI     SmlI     SspI     StyI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AgeI     AhdI     AleI
     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BpmI    BpuEI     BsaI    BsaHI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI   DraIII     DrdI     EagI     EciI
  EcoICRI     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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