Restriction map for pF1KB9339
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

Acc65I      AclI        AflII       Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BamHI       BbrPI       
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsiEI       BsiWI       Bsp1407I    
BspDI       BsrGI       BssHII      BssSI       BstBI       
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FbaI        FseI        FspI        HpaI        KpnI        
MamI        MluI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SalI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SspBI       SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1816  GTATAC               GT^[AC][GT]AC
AccIII       2963  TCCGGA               T^CCGGA
AgeI         1651  ACCGGT               A^CCGGT
AocI         3145  CCTGAGG              CC^T[ACGT]AGG
ApaI         2504  GGGCCC               GGGCC^C
BcgI         3434  GCACACTGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1198  CTAG                 C^TAG
BseAI        2963  TCCGGA               T^CCGGA
BspEI        2963  TCCGGA               T^CCGGA
BspHI        3308  TCATGA               T^CATGA
BspLU11I     3224  ACATGT               A^CATGT
Bst1107I     1816  GTATAC               GTA^TAC
BstAPI       3623  GCATGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1654  GGTGACC              G^GT[ACGT]ACC
BstPI        1654  GGTGACC              G^GT[ACGT]ACC
BstZ17I      1816  GTATAC               GTA^TAC
Bsu36I       3145  CCTGAGG              CC^T[ACGT]AGG
DrdI          792  GACTGGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3145  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1654  GGTGACC              G^GT[ACGT]ACC
HindIII      2114  AAGCTT               A^AGCTT
KspI         2637  CCGCGG               CCGC^GG
MaeI         1198  CTAG                 C^TAG
MfeI          395  CAATTG               C^AATTG
MroI         2963  TCCGGA               T^CCGGA
MunI          395  CAATTG               C^AATTG
NaeI         2036  GCCGGC               GCC^GGC
NcoI         2210  CCATGG               C^CATGG
NdeI         1070  CATATG               CA^TATG
NgoMIV       2036  GCCGGC               G^CCGGC
PinAI        1651  ACCGGT               A^CCGGT
PshAI         683  GACGTCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         3308  TCATGA               T^CATGA
SacII        2637  CCGCGG               CCGC^GG
SbfI         3525  CCTGCAGG             CCTGCA^GG
ScaI         3428  AGTACT               AGT^ACT
SmaI         3038  CCCGGG               CCC^GGG
SphI         3623  GCATGC               GCATG^C
Sse8387I     3525  CCTGCAGG             CCTGCA^GG
StuI         1907  AGGCCT               AGG^CCT
XmaCI        3038  CCCGGG               C^CCGGG
XmaI         3038  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AatII         683  GACGTC               GACGT^C
             2632  GACGTC              
AflIII       2831  ACACGT               A^C[GA][CT]GT
             3224  ACATGT              
AhdI         2557  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3445  GACAGCTCGTC         
Asp700I      2829  GAACACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3330  GAATAACTTC          
AspEI        2557  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3445  GACAGCTCGTC         
BalI         2379  TGGCCA               TGG^CCA
             3350  TGGCCA              
BanI          930  GGCGCC               G^G[CT][GA]CC
             3375  GGCGCC              
BbeI          930  GGCGCC               GGCGC^C
             3375  GGCGCC              
BglI         2036  GCCGGCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3466  GCCTACCGGGC         
BsaI          299  GGTCTC               GGTCTC(1/5)
             1316  GGTCTC              
BsmI          872  GCATTC               GAATGC(1/-1)
             2713  GCATTC              
BsrDI         905  CATTGC               GCAATG(2/0)
             2933  GCAATG              
Eam1105I     2557  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3445  GACAGCTCGTC         
EcoRI         482  GAATTC               G^AATTC
             1222  GAATTC              
EcoT14I       242  CCTTGG               C^C[AT][AT]GG
             2210  CCATGG              
HaeII         930  GGCGCC               [GA]GCGC^[CT]
             3375  GGCGCC              
KasI          930  GGCGCC               G^GCGCC
             3375  GGCGCC              
MluNI        2379  TGGCCA               TGG^CCA
             3350  TGGCCA              
MscI         2379  TGGCCA               TGG^CCA
             3350  TGGCCA              
MseI          371  TTAA                 T^TAA
             3743  TTAA                
NarI          930  GGCGCC               GG^CGCC
             3375  GGCGCC              
PaeR7I       2319  CTCGAG               C^TCGAG
             2408  CTCGAG              
SapI         3183  GAAGAGC              GCTCTTC(1/4)
             3123  GCTCTTC             
SfoI          930  GGCGCC               GGC^GCC
             3375  GGCGCC              
StyI          242  CCTTGG               C^C[AT][AT]GG
             2210  CCATGG              
Tru9I         371  TTAA                 T^TAA
             3743  TTAA                
XhoI         2319  CTCGAG               C^TCGAG
             2408  CTCGAG              
XmnI         2829  GAACACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3330  GAATAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanII       BbsI        BbvCI       
BbvI        BcnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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