Restriction map for pF1KB9339
 (Linear) (Six-base) MAPSORT of: KIBB9339.seq  Check: 9145  from: 1  to: 3792



KIBB9339.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1419    2178    3792 
   Size:       1419     759    1614

  Fragments arranged by size: 

                1614    1419     759
 
AatII G_ACGT'C

Cuts at:      0     687    2636    3792 
   Size:        687    1949    1156

  Fragments arranged by size: 

                1949    1156     687
 
AccI GT'mk_AC

Cuts at:      0    1817    3792 
   Size:       1817    1975
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1809    1821    2917    3395    3678    3792 
   Size:       1809      12    1096     478     283     114

  Fragments arranged by size: 

                1809    1096     478     283     114      12
 
AflIII A'CryG_T

Cuts at:      0    2831    3224    3792 
   Size:       2831     393     568

  Fragments arranged by size: 

                2831     568     393
 
AgeI A'CCGG_T

Cuts at:      0    1651    3792 
   Size:       1651    2141
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2562    3450    3792 
   Size:       2562     888     342

  Fragments arranged by size: 

                2562     888     342
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2903    2935    3792 
   Size:       2903      32     857

  Fragments arranged by size: 

                2903     857      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1612    1722    1863    3030    3534    3792 
   Size:       1612     110     141    1167     504     258

  Fragments arranged by size: 

                1612    1167     504     258     141     110
 
ApaI G_GGCC'C

Cuts at:      0    2508    3792 
   Size:       2508    1284
 
ApoI r'AATT_y

Cuts at:      0     109     268     482    1222    1618    2785    3792 
   Size:        109     159     214     740     396    1167    1007

  Fragments arranged by size: 

                1167    1007     740     396     214     159     109
 
AvaI C'yCGr_G

Cuts at:      0     944    1420    1978    2319    2408    3038    3792 
   Size:        944     476     558     341      89     630     754

  Fragments arranged by size: 

                 944     754     630     558     476     341      89
 
BanI G'GyrC_C

Cuts at:      0     930    3375    3792 
   Size:        930    2445     417

  Fragments arranged by size: 

                2445     930     417
 
BanII G_rGCy'C

Cuts at:      0     703     752     818    2191    2508    2756    2867    3125 
   Size:        703      49      66    1373     317     248     111     258

Cuts at:   3125    3190    3792 
   Size:         65     602

  Fragments arranged by size: 

                1373     703     602     317     258     248     111      66
                  65      49
 
BbeI G_GCGC'C

Cuts at:      0     934    3379    3792 
   Size:        934    2445     413

  Fragments arranged by size: 

                2445     934     413
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     596    2910    3792 
   Size:        596    2314     882

  Fragments arranged by size: 

                2314     882     596
 
BbvCI CC'TCA_GC

Cuts at:      0     161    1262    1605    2454    3710    3792 
   Size:        161    1101     343     849    1256      82

  Fragments arranged by size: 

                1256    1101     849     343     161      82
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3423    3457    3792 
   Size:       3423      34     335

  Fragments arranged by size: 

                3423     335      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2042    3472    3792 
   Size:       2042    1430     320

  Fragments arranged by size: 

                2042    1430     320
 
BlpI GC'TnA_GC

Cuts at:      0     495    1120    2944    3119    3792 
   Size:        495     625    1824     175     673

  Fragments arranged by size: 

                1824     673     625     495     175
 
Bme1580I G_kGCm'C

Cuts at:      0     360    2060    2508    3792 
   Size:        360    1700     448    1284

  Fragments arranged by size: 

                1700    1284     448     360
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     707     803    3195    3243    3792 
   Size:        707      96    2392      48     549

  Fragments arranged by size: 

                2392     707     549      96      48
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1749    2805    3792 
   Size:       1749    1056     987

  Fragments arranged by size: 

                1749    1056     987
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     349     433     466     972    1213    1744    2548 
   Size:        271      78      84      33     506     241     531     804

Cuts at:   2548    3003    3412    3792 
   Size:        455     409     380

  Fragments arranged by size: 

                 804     531     506     455     409     380     271     241
                  84      78      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     161     319    1262    1282    1605    1997    2454    3710 
   Size:        161     158     943      20     323     392     457    1256

Cuts at:   3710    3792 
   Size:         82

  Fragments arranged by size: 

                1256     943     457     392     323     161     158      82
                  20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2590    3792 
   Size:       2590    1202
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     305    1322    3792 
   Size:        305    1017    2470

  Fragments arranged by size: 

                2470    1017     305
 
BsaHI Gr'CG_yC

Cuts at:      0     684     931    1765    2468    2633    3376    3792 
   Size:        684     247     834     703     165     743     416

  Fragments arranged by size: 

                 834     743     703     684     416     247     165
 
BsaWI w'CCGG_w

Cuts at:      0    1651    2963    3239    3792 
   Size:       1651    1312     276     553

  Fragments arranged by size: 

                1651    1312     553     276
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1300    1330    1400    1430    3325    3355    3792 
   Size:       1300      30      70      30    1895      30     437

  Fragments arranged by size: 

                1895    1300     437      70      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     346     808     851    1664    1795    1923    2136    2407 
   Size:        346     462      43     813     131     128     213     271

Cuts at:   2407    2426    2560    2764    3193    3727    3792 
   Size:         19     134     204     429     534      65

  Fragments arranged by size: 

                 813     534     462     429     346     271     213     204
                 134     131     128      65      43      19
 
BseYI C'CCAG_C

Cuts at:      0     741    1364    2227    2425    3792 
   Size:        741     623     863     198    1367

  Fragments arranged by size: 

                1367     863     741     623     198
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487    1876    3417    3792 
   Size:        487    1389    1541     375

  Fragments arranged by size: 

                1541    1389     487     375
 
BsiHKAI G_wGCw'C

Cuts at:      0     752     818    1174    1537    1781    2191    2756    2867 
   Size:        752      66     356     363     244     410     565     111

Cuts at:   2867    2963    3125    3215    3792 
   Size:         96     162      90     577

  Fragments arranged by size: 

                 752     577     565     410     363     356     244     162
                 111      96      90      66
 
BsmI GAATG_Cn'

Cuts at:      0     872    2713    3792 
   Size:        872    1841    1079

  Fragments arranged by size: 

                1841    1079     872
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     695    2022    2483    3792 
   Size:        695    1327     461    1309

  Fragments arranged by size: 

                1327    1309     695     461
 
Bsp1286I G_dGCh'C

Cuts at:      0     360     703     752     818    1174    1537    1781    2060 
   Size:        360     343      49      66     356     363     244     279

Cuts at:   2060    2191    2508    2756    2867    2963    3125    3190    3215 
   Size:        131     317     248     111      96     162      65      25

Cuts at:   3215    3792 
   Size:        577

  Fragments arranged by size: 

                 577     363     360     356     343     317     279     248
                 244     162     131     111      96      66      65      49
                  25
 
BspEI T'CCGG_A

Cuts at:      0    2963    3792 
   Size:       2963     829
 
BspHI T'CATG_A

Cuts at:      0    3308    3792 
   Size:       3308     484
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1419    1535    2178    3792 
   Size:       1419     116     643    1614

  Fragments arranged by size: 

                1614    1419     643     116
 
BsrBI CCG'CTC

Cuts at:      0       5    1488    1996    3792 
   Size:          5    1483     508    1796

  Fragments arranged by size: 

                1796    1483     508       5
 
BsrDI GCAATG_nn'

Cuts at:      0     904    2940    3792 
   Size:        904    2036     852

  Fragments arranged by size: 

                2036     904     852
 
BsrFI r'CCGG_y

Cuts at:      0    1651    2036    2215    2421    3792 
   Size:       1651     385     179     206    1371

  Fragments arranged by size: 

                1651    1371     385     206     179
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3629    3792 
   Size:       3629     163
 
BstEII G'GTnAC_C

Cuts at:      0    1654    3792 
   Size:       1654    2138
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288    1072    2235    3792 
   Size:        288     784    1163    1557

  Fragments arranged by size: 

                1557    1163     784     288
 
BstYI r'GATC_y

Cuts at:      0     246     776    2448    2644    2891    3792 
   Size:        246     530    1672     196     247     901

  Fragments arranged by size: 

                1672     901     530     247     246     196
 
BstZ17I GTA'TAC

Cuts at:      0    1818    3792 
   Size:       1818    1974
 
Bsu36I CC'TnA_GG

Cuts at:      0    3146    3792 
   Size:       3146     646
 
BtgI C'CryG_G

Cuts at:      0     830    2210    2234    2637    3792 
   Size:        830    1380      24     403    1155

  Fragments arranged by size: 

                1380    1155     830     403      24
 
BtsI GCAGTG_nn'

Cuts at:      0    1174    1995    2502    3792 
   Size:       1174     821     507    1290

  Fragments arranged by size: 

                1290    1174     821     507
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     887     922    3792 
   Size:        887      35    2870

  Fragments arranged by size: 

                2870     887      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     798    3792 
   Size:        798    2994
 
EaeI y'GGCC_r

Cuts at:      0    1356    2213    2379    3350    3422    3792 
   Size:       1356     857     166     971      72     370

  Fragments arranged by size: 

                1356     971     857     370     166      72
 
EarI CTCTTCn'nnn_

Cuts at:      0      87     510     833    2154    2882    3130    3178    3776 
   Size:         87     423     323    1321     728     248      48     598

Cuts at:   3776    3792 
   Size:         16

  Fragments arranged by size: 

                1321     728     598     423     323     248      87      48
                  16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1471    2114    2126    2911    3792 
   Size:       1471     643      12     785     881

  Fragments arranged by size: 

                1471     881     785     643      12
 
EcoICRI GAG'CTC

Cuts at:      0     750     816    2189    2754    2865    3123    3792 
   Size:        750      66    1373     565     111     258     669

  Fragments arranged by size: 

                1373     750     669     565     258     111      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     349     433     466     972    1213    1744    1809 
   Size:        271      78      84      33     506     241     531      65

Cuts at:   1809    1821    2548    2917    3003    3395    3412    3678    3792 
   Size:         12     727     369      86     392      17     266     114

  Fragments arranged by size: 

                 727     531     506     392     369     271     266     241
                 114      86      84      78      65      33      17      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1810    2980    3484    3709    3792 
   Size:       1810    1170     504     225      83

  Fragments arranged by size: 

                1810    1170     504     225      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1524    2001    2081    2504    2505    2732    3062    3417 
   Size:       1524     477      80     423       1     227     330     355

Cuts at:   3417    3531    3717    3792 
   Size:        114     186      75

  Fragments arranged by size: 

                1524     477     423     355     330     227     186     114
                  80      75       1
 
EcoRI G'AATT_C

Cuts at:      0     482    1222    3792 
   Size:        482     740    2570

  Fragments arranged by size: 

                2570     740     482
 
HaeII r_GCGC'y

Cuts at:      0     934    3379    3792 
   Size:        934    2445     413

  Fragments arranged by size: 

                2445     934     413
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1126    1158    1300    1332    1498    1530    2548    2580 
   Size:       1126      32     142      32     166      32    1018      32

Cuts at:   2580    3436    3468    3792 
   Size:        856      32     324

  Fragments arranged by size: 

                1126    1018     856     324     166     142      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     809    1029    1649    2882    3792 
   Size:        809     220     620    1233     910

  Fragments arranged by size: 

                1233     910     809     620     220
 
HindIII A'AGCT_T

Cuts at:      0    2114    3792 
   Size:       2114    1678
 
KasI G'GCGC_C

Cuts at:      0     930    3375    3792 
   Size:        930    2445     417

  Fragments arranged by size: 

                2445     930     417
 
MfeI C'AATT_G

Cuts at:      0     395    3792 
   Size:        395    3397
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     718     981    2522    3792 
   Size:        718     263    1541    1270

  Fragments arranged by size: 

                1541    1270     718     263
 
MscI TGG'CCA

Cuts at:      0    2381    3352    3792 
   Size:       2381     971     440

  Fragments arranged by size: 

                2381     971     440
 
MslI CAynn'nnrTG

Cuts at:      0     877    1070    3083    3250    3792 
   Size:        877     193    2013     167     542

  Fragments arranged by size: 

                2013     877     542     193     167
 
MspA1I CmG'CkG

Cuts at:      0     160    1140    1344    1896    1902    2458    2465    2639 
   Size:        160     980     204     552       6     556       7     174

Cuts at:   2639    3792 
   Size:       1153

  Fragments arranged by size: 

                1153     980     556     552     204     174     160       7
                   6
 
NaeI GCC'GGC

Cuts at:      0    2038    3792 
   Size:       2038    1754
 
NarI GG'CG_CC

Cuts at:      0     931    3376    3792 
   Size:        931    2445     416

  Fragments arranged by size: 

                2445     931     416
 
NcoI C'CATG_G

Cuts at:      0    2210    3792 
   Size:       2210    1582
 
NdeI CA'TA_TG

Cuts at:      0    1071    3792 
   Size:       1071    2721
 
NgoMIV G'CCGG_C

Cuts at:      0    2036    3792 
   Size:       2036    1756
 
NspI r_CATG'y

Cuts at:      0    1704    1716    3228    3627    3657    3792 
   Size:       1704      12    1512     399      30     135

  Fragments arranged by size: 

                1704    1512     399     135      30      12
 
PasI CC'CwG_GG

Cuts at:      0    1387    2473    3792 
   Size:       1387    1086    1319

  Fragments arranged by size: 

                1387    1319    1086
 
PciI A'CATG_T

Cuts at:      0    3224    3792 
   Size:       3224     568
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1327    2425    3030    3596    3792 
   Size:       1327    1098     605     566     196

  Fragments arranged by size: 

                1327    1098     605     566     196
 
PfoI T'CCnGG_A

Cuts at:      0    1174    2975    3792 
   Size:       1174    1801     817

  Fragments arranged by size: 

                1801    1174     817
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3272    3304    3792 
   Size:       3272      32     488

  Fragments arranged by size: 

                3272     488      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1524    2081    2732    3062    3531    3792 
   Size:       1524     557     651     330     469     261

  Fragments arranged by size: 

                1524     651     557     469     330     261
 
PshAI GACnn'nnGTC

Cuts at:      0     687    3792 
   Size:        687    3105
 
PsiI TTA'TAA

Cuts at:      0    3676    3792 
   Size:       3676     116
 
PspOMI G'GGCC_C

Cuts at:      0    2504    3792 
   Size:       2504    1288
 
PspXI vC'TCGA_Gb

Cuts at:      0    2319    3792 
   Size:       2319    1473
 
PstI C_TGCA'G

Cuts at:      0    1105    1474    1532    1907    2771    3530    3792 
   Size:       1105     369      58     375     864     759     262

  Fragments arranged by size: 

                1105     864     759     375     369     262      58
 
PvuII CAG'CTG

Cuts at:      0     160    1140    1344    1902    3792 
   Size:        160     980     204     558    1890

  Fragments arranged by size: 

                1890     980     558     204     160
 
SacI G_AGCT'C

Cuts at:      0     752     818    2191    2756    2867    3125    3792 
   Size:        752      66    1373     565     111     258     667

  Fragments arranged by size: 

                1373     752     667     565     258     111      66
 
SacII CC_GC'GG

Cuts at:      0    2640    3792 
   Size:       2640    1152
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3130    3178    3792 
   Size:       3130      48     614

  Fragments arranged by size: 

                3130     614      48
 
SbfI CC_TGCA'GG

Cuts at:      0    3530    3792 
   Size:       3530     262
 
ScaI AGT'ACT

Cuts at:      0    3430    3792 
   Size:       3430     362
 
SfcI C'TryA_G

Cuts at:      0     513    1101    1470    1528    1903    2727    2767    3526 
   Size:        513     588     369      58     375     824      40     759

Cuts at:   3526    3792 
   Size:        266

  Fragments arranged by size: 

                 824     759     588     513     375     369     266      58
                  40
 
SfoI GGC'GCC

Cuts at:      0     932    3377    3792 
   Size:        932    2445     415

  Fragments arranged by size: 

                2445     932     415
 
SmaI CCC'GGG

Cuts at:      0    3040    3792 
   Size:       3040     752
 
SmlI C'TyrA_G

Cuts at:      0    2319    2408    2605    3792 
   Size:       2319      89     197    1187

  Fragments arranged by size: 

                2319    1187     197      89
 
SphI G_CATG'C

Cuts at:      0    3627    3792 
   Size:       3627     165
 
StuI AGG'CCT

Cuts at:      0    1909    3792 
   Size:       1909    1883
 
StyI C'CwwG_G

Cuts at:      0     242    2210    3792 
   Size:        242    1968    1582

  Fragments arranged by size: 

                1968    1582     242
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     135     927    1615    3792 
   Size:        135     792     688    2177

  Fragments arranged by size: 

                2177     792     688     135
 
TatI w'GTAC_w

Cuts at:      0    2702    2771    3428    3792 
   Size:       2702      69     657     364

  Fragments arranged by size: 

                2702     657     364      69
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1400    1432    2101    2133    3484    3516    3792 
   Size:       1400      32     669      32    1351      32     276

  Fragments arranged by size: 

                1400    1351     669     276      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      99     285     441     459    1072    1348    3027    3387 
   Size:         99     186     156      18     613     276    1679     360

Cuts at:   3387    3792 
   Size:        405

  Fragments arranged by size: 

                1679     613     405     360     276     186     156      99
                  18
 
XhoI C'TCGA_G

Cuts at:      0    2319    2408    3792 
   Size:       2319      89    1384

  Fragments arranged by size: 

                2319    1384      89
 
XmaI C'CCGG_G

Cuts at:      0    3038    3792 
   Size:       3038     754
 
XmnI GAAnn'nnTTC

Cuts at:      0    2833    3334    3792 
   Size:       2833     501     458

  Fragments arranged by size: 

                2833     501     458
 
ZraI GAC'GTC

Cuts at:      0     685    2634    3792 
   Size:        685    1949    1158

  Fragments arranged by size: 

                1949    1158     685
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AgeI     AhdI     AloI
    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI    CspCI     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I   HincII
  HindIII     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII     SapI     SbfI     ScaI     SfcI     SfoI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AleI     AlfI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BsrGI
   BssHII    BssSI    BstBI    BtgZI     ClaI     DraI   DraIII     EagI
    EcoRV     FalI     FseI     FspI    FspAI     HpaI     KpnI     MluI
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI     PsrI
     PvuI    RsrII     SalI    SanDI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI
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