Restriction map for pF1KB9131
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AvaI        AviII       AvrII       
BalI        BanI        BbeI        BbrPI       BbvCI       
BcgI        BcnI        BfaI        BfrI        BglI        
BlnI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
Bsu36I      CfoI        Cfr10I      CpoI        DraIII      
DrdI        DsaI        EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
EcoT14I     FauI        FseI        FspI        HaeII       
HapII       HhaI        HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       StyI        
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI          902  GCGG                 CCGC(-3/-1)
AcyI          327  GGCGTC               G[GA]^CG[CT]C
AhdI          258  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         258  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1297  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        1610  GGACC                G^G[AT]CC
BamHI        1176  GGATCC               G^GATCC
BbsI          585  GAAGAC               GAAGAC(2/6)
BciVI         786  GGATAC               GTATCC(6/5)
BclI          894  TGATCA               T^GATCA
BpuAI         585  GAAGAC               GAAGAC(2/6)
BsaHI         327  GGCGTC               G[GA]^CG[CT]C
BsmAI        1164  GTCTC                GTCTC(1/5)
BsmFI        1321  GGGAC                GGGAC(10/14)
BspDI        1544  ATCGAT               AT^CGAT
BstEII        768  GGTGACC              G^GT[ACGT]ACC
BstPI         768  GGTGACC              G^GT[ACGT]ACC
Cfr13I       1610  GGACC                G^G[ACGT]CC
ClaI         1544  ATCGAT               AT^CGAT
DraII        1609  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      258  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1308  CCTTTTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1609  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       768  GGTGACC              G^GT[ACGT]ACC
FbaI          894  TGATCA               T^GATCA
HgaI          328  GCGTC                GACGC(5/10)
Hin1I         327  GGCGTC               G[GA]^CG[CT]C
NdeI         1169  CATATG               CA^TATG
NlaIV        1176  GGATCC               GG[ACGT]^[ACGT]CC
PflFI        1297  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1609  AGGACCT              [GA]G^G[AT]CC[CT]
PstI         1538  CTGCAG               CTGCA^G
SapI          719  GCTCTTC              GCTCTTC(1/4)
Sau96I       1610  GGACC                G^G[ACGT]CC
SexAI        1298  ACCAGGT              A^CC[AT]GGT
SmlI           54  CTCAAG               C^T[CT][GA]AG
SphI          833  GCATGC               GCATG^C
SspI          184  AATATT               AAT^ATT
StuI         1640  AGGCCT               AGG^CCT
SwaI          884  ATTTAAAT             ATTT^AAAT
Tth111I      1297  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          686  CCAAGCCCCAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Alw44I        245  GTGCAC               G^TGCAC
             1601  GTGCAC              
ApaLI         245  GTGCAC               G^TGCAC
             1601  GTGCAC              
Asp700I        90  GAATAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              853  GAACGGATTC          
BanII         776  GAGCTC               G[GA]GC[CT]^C
             1711  GAGCTC              
BglII        1281  AGATCT               A^GATCT
             1677  AGATCT              
BlpI           47  GCTCAGC              GC^T[ACGT]AGC
              912  GCTTAGC             
Bpu1102I       47  GCTCAGC              GC^T[ACGT]AGC
              912  GCTTAGC             
BsmI            9  GAATGC               GAATGC(1/-1)
             1136  GAATGC              
BspHI         645  TCATGA               T^CATGA
              891  TCATGA              
BstXI         603  CCAGTTCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              863  CCAAGAACATGG        
CelII          47  GCTCAGC              GC^T[ACGT]AGC
              912  GCTTAGC             
EcoT22I       982  ATGCAT               ATGCA^T
             1138  ATGCAT              
HaeIII       1641  GGCC                 GG^CC
             1719  GGCC                
HincII       1303  GTCAAC               GT[CT]^[GA]AC
             1564  GTCAAC              
HindII       1303  GTCAAC               GT[CT]^[GA]AC
             1564  GTCAAC              
HinfI         335  GATTC                G^A[ACGT]TC
              858  GATTC               
MaeII        1374  ACGT                 A^CGT
             1562  ACGT                
MwoI          658  GCCTTGCCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              681  GCGACCCAAGC         
NsiI          982  ATGCAT               ATGCA^T
             1138  ATGCAT              
NspI          833  GCATGC               [GA]CATG^[CT]
             1697  ACATGC              
RcaI          645  TCATGA               T^CATGA
              891  TCATGA              
SacI          776  GAGCTC               GAGCT^C
             1711  GAGCTC              
SfcI          930  CTGTAG               C^T[GA][CT]AG
             1538  CTGCAG              
TfiI          335  GATTC                G^A[AT]TC
              858  GATTC               
XmnI           90  GAATAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              853  GAACGGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AspHI       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       DdeI        DpnII       DraI        
EarI        EcoRII      Fnu4HI      FokI        HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        NdeII       NlaIII      PvuII       RsaI        
Sau3AI      ScrFI       SfaNI       TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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