Restriction map for pF1KB9128
 (Linear) (Six-base) MAPSORT of: KIBB9128.seq  Check: 1236  from: 1  to: 2706



KIBB9128.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:01  ..

AccI GT'mk_AC

Cuts at:      0    1474    2706 
   Size:       1474    1232
 
Acc65I G'GTAC_C

Cuts at:      0    1188    2706 
   Size:       1188    1518
 
AclI AA'CG_TT

Cuts at:      0     115    2706 
   Size:        115    2591
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     599    1957    2422    2618    2706 
   Size:        407     192    1358     465     196      88

  Fragments arranged by size: 

                1358     465     407     196     192      88
 
AflII C'TTAA_G

Cuts at:      0    1069    2706 
   Size:       1069    1637
 
AleI CACnn'nnGTG

Cuts at:      0    2512    2706 
   Size:       2512     194
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1982    2706 
   Size:       1982     724
 
AlwNI CAG_nnn'CTG

Cuts at:      0     534    2706 
   Size:        534    2172
 
ApoI r'AATT_y

Cuts at:      0     180     281     353     586     681     984    1042    1203 
   Size:        180     101      72     233      95     303      58     161

Cuts at:   1203    1591    2706 
   Size:        388    1115

  Fragments arranged by size: 

                1115     388     303     233     180     161     101      95
                  72      58
 
AseI AT'TA_AT

Cuts at:      0     449    2706 
   Size:        449    2257
 
BanI G'GyrC_C

Cuts at:      0    1188    1341    2706 
   Size:       1188     153    1365

  Fragments arranged by size: 

                1365    1188     153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     904    1090    1306    2706 
   Size:        904     186     216    1400

  Fragments arranged by size: 

                1400     904     216     186
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     830    1249    2706 
   Size:        830     419    1457

  Fragments arranged by size: 

                1457     830     419
 
BfrBI ATG'CAT

Cuts at:      0     864    1959    2706 
   Size:        864    1095     747

  Fragments arranged by size: 

                1095     864     747
 
Bme1580I G_kGCm'C

Cuts at:      0    2048    2706 
   Size:       2048     658
 
BmgBI CAC'GTC

Cuts at:      0    2327    2706 
   Size:       2327     379
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     702    2011    2706 
   Size:        702    1309     695

  Fragments arranged by size: 

                1309     702     695
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1365    1625    2706 
   Size:       1365     260    1081

  Fragments arranged by size: 

                1365    1081     260
 
Bpu10I CC'TnA_GC

Cuts at:      0    1064    2706 
   Size:       1064    1642
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     623    1081    1632    2706 
   Size:        449     174     458     551    1074

  Fragments arranged by size: 

                1074     551     458     449     174
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2670    2706 
   Size:       2670      36
 
BsaAI yAC'GTr

Cuts at:      0    1425    2706 
   Size:       1425    1281
 
BsiHKAI G_wGCw'C

Cuts at:      0    1635    2706 
   Size:       1635    1071
 
BsmI GAATG_Cn'

Cuts at:      0      51    1756    2706 
   Size:         51    1705     950

  Fragments arranged by size: 

                1705     950      51
 
Bsp1286I G_dGCh'C

Cuts at:      0    1635    2048    2706 
   Size:       1635     413     658

  Fragments arranged by size: 

                1635     658     413
 
BspHI T'CATG_A

Cuts at:      0    2672    2706 
   Size:       2672      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2613    2706 
   Size:       2613      93
 
BsrBI CCG'CTC

Cuts at:      0    1498    2706 
   Size:       1498    1208
 
BsrDI GCAATG_nn'

Cuts at:      0     789    1928    2706 
   Size:        789    1139     778

  Fragments arranged by size: 

                1139     789     778
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     295     534    1918    2706 
   Size:        295     239    1384     788

  Fragments arranged by size: 

                1384     788     295     239
 
BstBI TT'CG_AA

Cuts at:      0    1113    2706 
   Size:       1113    1593
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1138    2511    2706 
   Size:       1138    1373     195

  Fragments arranged by size: 

                1373    1138     195
 
BstYI r'GATC_y

Cuts at:      0    1891    2706 
   Size:       1891     815
 
BstZ17I GTA'TAC

Cuts at:      0    1475    2706 
   Size:       1475    1231
 
BtsI GCAGTG_nn'

Cuts at:      0    1030    2706 
   Size:       1030    1676
 
DraI TTT'AAA

Cuts at:      0    1415    2193    2706 
   Size:       1415     778     513

  Fragments arranged by size: 

                1415     778     513
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     413    2706 
   Size:        413    2293
 
EarI CTCTTCn'nnn_

Cuts at:      0    1354    2441    2706 
   Size:       1354    1087     265

  Fragments arranged by size: 

                1354    1087     265
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     599    1365    1625    1957    2422    2618    2706 
   Size:        407     192     766     260     332     465     196      88

  Fragments arranged by size: 

                 766     465     407     332     260     196     192      88
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     877    2706 
   Size:        877    1829
 
EcoO109I rG'GnC_Cy

Cuts at:      0     851    1654    2666    2706 
   Size:        851     803    1012      40

  Fragments arranged by size: 

                1012     851     803      40
 
EcoRI G'AATT_C

Cuts at:      0    1591    2706 
   Size:       1591    1115
 
FspI TGC'GCA

Cuts at:      0    1755    2706 
   Size:       1755     951
 
FspAI rTGC'GCAy

Cuts at:      0    1755    2706 
   Size:       1755     951
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     253     285     621     653     682     714    1299    1331 
   Size:        253      32     336      32      29      32     585      32

Cuts at:   1331    1494    1509    1526    1541    2706 
   Size:        163      15      17      15    1165

  Fragments arranged by size: 

                1165     585     336     253     163      32      32      32
                  32      29      17      15      15
 
HindIII A'AGCT_T

Cuts at:      0     733    1099    2706 
   Size:        733     366    1607

  Fragments arranged by size: 

                1607     733     366
 
KpnI G_GTAC'C

Cuts at:      0    1192    2706 
   Size:       1192    1514
 
MfeI C'AATT_G

Cuts at:      0    2531    2706 
   Size:       2531     175
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1233    2182    2199    2666    2706 
   Size:       1233     949      17     467      40

  Fragments arranged by size: 

                1233     949     467      40      17
 
MslI CAynn'nnrTG

Cuts at:      0     752    2512    2706 
   Size:        752    1760     194

  Fragments arranged by size: 

                1760     752     194
 
MspA1I CmG'CkG

Cuts at:      0     334    2706 
   Size:        334    2372
 
NsiI A_TGCA'T

Cuts at:      0     866    1961    2706 
   Size:        866    1095     745

  Fragments arranged by size: 

                1095     866     745
 
NspI r_CATG'y

Cuts at:      0    1168    1277    2706 
   Size:       1168     109    1429

  Fragments arranged by size: 

                1429    1168     109
 
PasI CC'CwG_GG

Cuts at:      0      61    2706 
   Size:         61    2645
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1185    2706 
   Size:       1185    1521
 
PfoI T'CCnGG_A

Cuts at:      0    1664    1763    2706 
   Size:       1664      99     943

  Fragments arranged by size: 

                1664     943      99
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1445    1477    2706 
   Size:       1445      32    1229

  Fragments arranged by size: 

                1445    1229      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     851    1654    2706 
   Size:        851     803    1052

  Fragments arranged by size: 

                1052     851     803
 
PshAI GACnn'nnGTC

Cuts at:      0    2682    2706 
   Size:       2682      24
 
PsiI TTA'TAA

Cuts at:      0    1012    2473    2706 
   Size:       1012    1461     233

  Fragments arranged by size: 

                1461    1012     233
 
PstI C_TGCA'G

Cuts at:      0    1037    2589    2706 
   Size:       1037    1552     117

  Fragments arranged by size: 

                1552    1037     117
 
PvuII CAG'CTG

Cuts at:      0     334    2706 
   Size:        334    2372
 
ScaI AGT'ACT

Cuts at:      0    2478    2706 
   Size:       2478     228
 
SfcI C'TryA_G

Cuts at:      0     173     632     874    1033    2585    2706 
   Size:        173     459     242     159    1552     121

  Fragments arranged by size: 

                1552     459     242     173     159     121
 
SmlI C'TyrA_G

Cuts at:      0     464     638    1069    1096    1611    2706 
   Size:        464     174     431      27     515    1095

  Fragments arranged by size: 

                1095     515     464     431     174      27
 
SspI AAT'ATT

Cuts at:      0    1055    1702    2349    2706 
   Size:       1055     647     647     357

  Fragments arranged by size: 

                1055     647     647     357
 
TatI w'GTAC_w

Cuts at:      0     468    2476    2706 
   Size:        468    2008     230

  Fragments arranged by size: 

                2008     468     230
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2511    2706 
   Size:       2511     195
 
XmnI GAAnn'nnTTC

Cuts at:      0     281     911     986    1109    2706 
   Size:        281     630      75     123    1597

  Fragments arranged by size: 

                1597     630     281     123      75
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII     AleI     AlfI    AlwNI
     ApoI     AseI     BanI     BbsI    BciVI    BfrBI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I     BtsI     DraI     DrdI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FspI    FspAI    Hin4I  HindIII     KpnI     MfeI
     MmeI     MslI   MspA1I     NsiI     NspI     PasI    PflMI     PfoI
     PpiI    PpuMI    PshAI     PsiI     PstI    PvuII     ScaI     SfcI
     SmlI     SspI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI   AflIII     AgeI     AhdI     AloI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI     BclI     BglI    BglII     BlpI     BmtI
     BplI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BsrFI    BsrGI   BssHII    BssSI
   BstEII   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     EaeI
     EagI     EciI  EcoICRI    EcoRV     FalI     FseI    HaeII   HincII
     HpaI     KasI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI     PmlI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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