Restriction map for pF1KB9098
 (Linear) (Six-base) MAPSORT of: KIBB9098.seq  Check: 9164  from: 1  to: 3771



KIBB9098.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     443    3771 
   Size:        443    3328
 
AatII G_ACGT'C

Cuts at:      0     857    3771 
   Size:        857    2914
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     541     940    1099    1851    2310    2414    2555 
   Size:        279     262     399     159     752     459     104     141

Cuts at:   2555    3050    3218    3771 
   Size:        495     168     553

  Fragments arranged by size: 

                 752     553     495     459     399     279     262     168
                 159     141     104
 
AfeI AGC'GCT

Cuts at:      0    2435    3771 
   Size:       2435    1336
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2618    3771 
   Size:       2618    1153
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1912    3771 
   Size:       1912    1859
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3746    3771 
   Size:       3746      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0     147     774    1038    3771 
   Size:        147     627     264    2733

  Fragments arranged by size: 

                2733     627     264     147
 
ApoI r'AATT_y

Cuts at:      0    1794    2565    3771 
   Size:       1794     771    1206

  Fragments arranged by size: 

                1794    1206     771
 
AseI AT'TA_AT

Cuts at:      0     415    3771 
   Size:        415    3356
 
AvaI C'yCGr_G

Cuts at:      0     171     186    3771 
   Size:        171      15    3585

  Fragments arranged by size: 

                3585     171      15
 
BamHI G'GATC_C

Cuts at:      0      11    1612    3771 
   Size:         11    1601    2159

  Fragments arranged by size: 

                2159    1601      11
 
BanI G'GyrC_C

Cuts at:      0    1395    1430    2698    3771 
   Size:       1395      35    1268    1073

  Fragments arranged by size: 

                1395    1268    1073      35
 
BanII G_rGCy'C

Cuts at:      0     178    1184    1772    2087    2452    2876    2927    3050 
   Size:        178    1006     588     315     365     424      51     123

Cuts at:   3050    3771 
   Size:        721

  Fragments arranged by size: 

                1006     721     588     424     365     315     178     123
                  51
 
BbeI G_GCGC'C

Cuts at:      0    1434    3771 
   Size:       1434    2337
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     466     602     673    1992    2429    3102    3584    3731 
   Size:        466     136      71    1319     437     673     482     147

Cuts at:   3731    3771 
   Size:         40

  Fragments arranged by size: 

                1319     673     482     466     437     147     136      71
                  40
 
BbvCI CC'TCA_GC

Cuts at:      0     325     752    2275    3771 
   Size:        325     427    1523    1496

  Fragments arranged by size: 

                1523    1496     427     325
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1311    1345    2871    2905    3771 
   Size:       1311      34    1526      34     866

  Fragments arranged by size: 

                1526    1311     866      34      34
 
BclI T'GATC_A

Cuts at:      0    1325    1820    2484    3771 
   Size:       1325     495     664    1287

  Fragments arranged by size: 

                1325    1287     664     495
 
BglI GCCn_nnn'nGGC

Cuts at:      0     189    2653    3771 
   Size:        189    2464    1118

  Fragments arranged by size: 

                2464    1118     189
 
BglII A'GATC_T

Cuts at:      0     902    3771 
   Size:        902    2869
 
BlpI GC'TnA_GC

Cuts at:      0    2437    3044    3771 
   Size:       2437     607     727

  Fragments arranged by size: 

                2437     727     607
 
Bme1580I G_kGCm'C

Cuts at:      0    1400    2701    3771 
   Size:       1400    1301    1070

  Fragments arranged by size: 

                1400    1301    1070
 
BmgBI CAC'GTC

Cuts at:      0      33    3392    3771 
   Size:         33    3359     379

  Fragments arranged by size: 

                3359     379      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2841    3771 
   Size:       2841     930
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     186    3366    3771 
   Size:        186    3180     405

  Fragments arranged by size: 

                3180     405     186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     286     766     918    1141    1267    1538    1951 
   Size:        223      63     480     152     223     126     271     413

Cuts at:   1951    2101    3028    3091    3361    3771 
   Size:        150     927      63     270     410

  Fragments arranged by size: 

                 927     480     413     410     271     270     223     223
                 152     150     126      63      63
 
Bpu10I CC'TnA_GC

Cuts at:      0      85     325     752    1957    2275    2733    3771 
   Size:         85     240     427    1205     318     458    1038

  Fragments arranged by size: 

                1205    1038     458     427     318     240      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     529    3771 
   Size:        529    3242
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1117    1445    2854    3771 
   Size:       1117     328    1409     917

  Fragments arranged by size: 

                1409    1117     917     328
 
BsaAI yAC'GTr

Cuts at:      0     765    3771 
   Size:        765    3006
 
BsaBI GATnn'nnATC

Cuts at:      0     508    2483    3771 
   Size:        508    1975    1288

  Fragments arranged by size: 

                1975    1288     508
 
BsaHI Gr'CG_yC

Cuts at:      0     854    1431    1832    3562    3771 
   Size:        854     577     401    1730     209

  Fragments arranged by size: 

                1730     854     577     401     209
 
BsaWI w'CCGG_w

Cuts at:      0     689    1298    2720    3771 
   Size:        689     609    1422    1051

  Fragments arranged by size: 

                1422    1051     689     609
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1240    1270    2074    2104    2517    2547    3746    3771 
   Size:       1240      30     804      30     413      30    1199      25

  Fragments arranged by size: 

                1240    1199     804     413      30      30      30      25
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1123    1264    1594    1612    2154    2485    3025    3145 
   Size:       1123     141     330      18     542     331     540     120

Cuts at:   3145    3568    3771 
   Size:        423     203

  Fragments arranged by size: 

                1123     542     540     423     331     330     203     141
                 120      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     646     760     787    1551    1969    2762    3771 
   Size:        415     231     114      27     764     418     793    1009

  Fragments arranged by size: 

                1009     793     764     418     415     231     114      27
            
 
BsiEI CG_ry'CG

Cuts at:      0     222    2657    3771 
   Size:        222    2435    1114

  Fragments arranged by size: 

                2435    1114     222
 
BsiHKAI G_wGCw'C

Cuts at:      0     178    1184    1208    2087    2452    2825    2876    2927 
   Size:        178    1006      24     879     365     373      51      51

Cuts at:   2927    3671    3771 
   Size:        744     100

  Fragments arranged by size: 

                1006     879     744     373     365     178     100      51
                  51      24
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1279    3771 
   Size:       1279    2492
 
Bsp1286I G_dGCh'C

Cuts at:      0     178    1184    1208    1400    1772    2087    2452    2701 
   Size:        178    1006      24     192     372     315     365     249

Cuts at:   2701    2825    2876    2927    3050    3671    3771 
   Size:        124      51      51     123     621     100

  Fragments arranged by size: 

                1006     621     372     365     315     249     192     178
                 124     123     100      51      51      24
 
BspEI T'CCGG_A

Cuts at:      0    1298    3771 
   Size:       1298    2473
 
BspHI T'CATG_A

Cuts at:      0    1200    1772    2609    3188    3771 
   Size:       1200     572     837     579     583

  Fragments arranged by size: 

                1200     837     583     579     572
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     155     206     437     443    1955    3771 
   Size:        155      51     231       6    1512    1816

  Fragments arranged by size: 

                1816    1512     231     155      51       6
 
BsrBI CCG'CTC

Cuts at:      0     516    1923    2940    3771 
   Size:        516    1407    1017     831

  Fragments arranged by size: 

                1407    1017     831     516
 
BsrDI GCAATG_nn'

Cuts at:      0     368    1770    3255    3771 
   Size:        368    1402    1485     516

  Fragments arranged by size: 

                1485    1402     516     368
 
BsrFI r'CCGG_y

Cuts at:      0     240     653    2210    3771 
   Size:        240     413    1557    1561

  Fragments arranged by size: 

                1561    1557     413     240
 
BssHII G'CGCG_C

Cuts at:      0      55     224    2145    3771 
   Size:         55     169    1921    1626

  Fragments arranged by size: 

                1921    1626     169      55
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     147     370     438     774    3771 
   Size:        147     223      68     336    2997

  Fragments arranged by size: 

                2997     336     223     147      68
 
BstEII G'GTnAC_C

Cuts at:      0     692    3771 
   Size:        692    3079
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2079    2767    3771 
   Size:       2079     688    1004

  Fragments arranged by size: 

                2079    1004     688
 
BstYI r'GATC_y

Cuts at:      0      11     902    1034    1151    1343    1612    1901    3771 
   Size:         11     891     132     117     192     269     289    1870

  Fragments arranged by size: 

                1870     891     289     269     192     132     117      11
            
 
BtgI C'CryG_G

Cuts at:      0     464    3771 
   Size:        464    3307
 
BtsI GCAGTG_nn'

Cuts at:      0    1044    2649    3372    3771 
   Size:       1044    1605     723     399

  Fragments arranged by size: 

                1605    1044     723     399
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     106     141    3771 
   Size:        106      35    3630

  Fragments arranged by size: 

                3630     106      35
 
DraI TTT'AAA

Cuts at:      0    1644    3771 
   Size:       1644    2127
 
DraIII CAC_nnn'GTG

Cuts at:      0    2835    3508    3771 
   Size:       2835     673     263

  Fragments arranged by size: 

                2835     673     263
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3586    3771 
   Size:       3586     185
 
EaeI y'GGCC_r

Cuts at:      0      27     251     467     815    1310    1617    2100    2267 
   Size:         27     224     216     348     495     307     483     167

Cuts at:   2267    2312    2654    3293    3771 
   Size:         45     342     639     478

  Fragments arranged by size: 

                 639     495     483     478     348     342     307     224
                 216     167      45      27
 
EagI C'GGCC_G

Cuts at:      0    2654    3771 
   Size:       2654    1117
 
EarI CTCTTCn'nnn_

Cuts at:      0     157    3771 
   Size:        157    3614
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     672    2229    2815    3771 
   Size:        672    1557     586     956

  Fragments arranged by size: 

                1557     956     672     586
 
EcoICRI GAG'CTC

Cuts at:      0     176    1182    2085    2450    2874    2925    3771 
   Size:        176    1006     903     365     424      51     846

  Fragments arranged by size: 

                1006     903     846     424     365     176      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     279     286     541     766     918     940    1099 
   Size:        223      56       7     255     225     152      22     159

Cuts at:   1099    1141    1267    1538    1851    1951    2101    2310    2414 
   Size:         42     126     271     313     100     150     209     104

Cuts at:   2414    2555    3028    3050    3091    3218    3361    3771 
   Size:        141     473      22      41     127     143     410

  Fragments arranged by size: 

                 473     410     313     271     255     225     223     209
                 159     152     150     143     141     127     126     104
                 100      56      42      41      22      22       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     202     544    1390    2932    3771 
   Size:        202     342     846    1542     839

  Fragments arranged by size: 

                1542     846     839     342     202
 
EcoO109I rG'GnC_Cy

Cuts at:      0     389    2256    2982    3176    3771 
   Size:        389    1867     726     194     595

  Fragments arranged by size: 

                1867     726     595     389     194
 
EcoRV GAT'ATC

Cuts at:      0    3614    3771 
   Size:       3614     157
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1548    3197    3771 
   Size:       1548    1649     574

  Fragments arranged by size: 

                1649    1548     574
 
FspI TGC'GCA

Cuts at:      0     715    3771 
   Size:        715    3056
 
HaeII r_GCGC'y

Cuts at:      0     247    1434    2437    3278    3771 
   Size:        247    1187    1003     841     493

  Fragments arranged by size: 

                1187    1003     841     493     247
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     520     552    1240    1272    2604    2636 
   Size:        160      32     328      32     688      32    1332      32

Cuts at:   2636    3771 
   Size:       1135

  Fragments arranged by size: 

                1332    1135     688     328     160      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     789    1857    3591    3771 
   Size:        789    1068    1734     180

  Fragments arranged by size: 

                1734    1068     789     180
 
HindIII A'AGCT_T

Cuts at:      0    1525    3419    3442    3771 
   Size:       1525    1894      23     329

  Fragments arranged by size: 

                1894    1525     329      23
 
KasI G'GCGC_C

Cuts at:      0    1430    3771 
   Size:       1430    2341
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1853    2560    3771 
   Size:       1853     707    1211

  Fragments arranged by size: 

                1853    1211     707
 
MscI TGG'CCA

Cuts at:      0      29     469    1312    2102    3771 
   Size:         29     440     843     790    1669

  Fragments arranged by size: 

                1669     843     790     440      29
 
MslI CAynn'nnrTG

Cuts at:      0    1393    1690    2302    3022    3209    3771 
   Size:       1393     297     612     720     187     562

  Fragments arranged by size: 

                1393     720     612     562     297     187
 
MspA1I CmG'CkG

Cuts at:      0     264     774     807     938    2279    3087    3314    3771 
   Size:        264     510      33     131    1341     808     227     457

  Fragments arranged by size: 

                1341     808     510     457     264     227     131      33
            
 
NaeI GCC'GGC

Cuts at:      0     242     655    2212    3771 
   Size:        242     413    1557    1559

  Fragments arranged by size: 

                1559    1557     413     242
 
NarI GG'CG_CC

Cuts at:      0    1431    3771 
   Size:       1431    2340
 
NdeI CA'TA_TG

Cuts at:      0    3307    3771 
   Size:       3307     464
 
NgoMIV G'CCGG_C

Cuts at:      0     240     653    2210    3771 
   Size:        240     413    1557    1561

  Fragments arranged by size: 

                1561    1557     413     240
 
NspI r_CATG'y

Cuts at:      0     379     714     744    2307    3771 
   Size:        379     335      30    1563    1464

  Fragments arranged by size: 

                1563    1464     379     335      30
 
PasI CC'CwG_GG

Cuts at:      0      42    3771 
   Size:         42    3729
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1746    3771 
   Size:       1746    2025
 
PfoI T'CCnGG_A

Cuts at:      0     677    1349    2021    2897    3771 
   Size:        677     672     672     876     874

  Fragments arranged by size: 

                 876     874     677     672     672
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1365    1397    3746    3771 
   Size:       1365      32    2349      25

  Fragments arranged by size: 

                2349    1365      32      25
 
PpuMI rG'GwC_Cy

Cuts at:      0     389    2982    3176    3771 
   Size:        389    2593     194     595

  Fragments arranged by size: 

                2593     595     389     194
 
PspXI vC'TCGA_Gb

Cuts at:      0     171    3771 
   Size:        171    3600
 
PstI C_TGCA'G

Cuts at:      0     143     443    1034    1901    2952    3771 
   Size:        143     300     591     867    1051     819

  Fragments arranged by size: 

                1051     867     819     591     300     143
 
PvuII CAG'CTG

Cuts at:      0     774     938    2279    3087    3771 
   Size:        774     164    1341     808     684

  Fragments arranged by size: 

                1341     808     774     684     164
 
SacI G_AGCT'C

Cuts at:      0     178    1184    2087    2452    2876    2927    3771 
   Size:        178    1006     903     365     424      51     844

  Fragments arranged by size: 

                1006     903     844     424     365     178      51
 
SapI GCTCTTCn'nnn_

Cuts at:      0     157    3771 
   Size:        157    3614
 
SexAI A'CCwGG_T

Cuts at:      0     725    3771 
   Size:        725    3046
 
SfcI C'TryA_G

Cuts at:      0     139     384     439    1030    1897    2948    3771 
   Size:        139     245      55     591     867    1051     823

  Fragments arranged by size: 

                1051     867     823     591     245     139      55
 
SfoI GGC'GCC

Cuts at:      0    1432    3771 
   Size:       1432    2339
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2210    3771 
   Size:       2210    1561
 
SmlI C'TyrA_G

Cuts at:      0     171     544    3771 
   Size:        171     373    3227

  Fragments arranged by size: 

                3227     373     171
 
SphI G_CATG'C

Cuts at:      0     714     744    3771 
   Size:        714      30    3027

  Fragments arranged by size: 

                3027     714      30
 
SspI AAT'ATT

Cuts at:      0    3691    3771 
   Size:       3691      80
 
StuI AGG'CCT

Cuts at:      0     917    3771 
   Size:        917    2854
 
StyI C'CwwG_G

Cuts at:      0     254     317     912    1799    3771 
   Size:        254      63     595     887    1972

  Fragments arranged by size: 

                1972     887     595     254      63
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     348     586    2559    2823    3279    3771 
   Size:        348     238    1973     264     456     492

  Fragments arranged by size: 

                1973     492     456     348     264     238
 
TatI w'GTAC_w

Cuts at:      0    1022    2306    3771 
   Size:       1022    1284    1465

  Fragments arranged by size: 

                1465    1284    1022
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      22      54    2821    2853    3494    3526    3771 
   Size:         22      32    2767      32     641      32     245

  Fragments arranged by size: 

                2767     641     245      32      32      32      22
 
Tth111I GACn'n_nGTC

Cuts at:      0     853    2196    3771 
   Size:        853    1343    1575

  Fragments arranged by size: 

                1575    1343     853
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     261     951    1242    1431    2013    2019    2500    3771 
   Size:        261     690     291     189     582       6     481    1271

  Fragments arranged by size: 

                1271     690     582     481     291     261     189       6
            
 
XhoI C'TCGA_G

Cuts at:      0     171    3771 
   Size:        171    3600
 
ZraI GAC'GTC

Cuts at:      0     855    3771 
   Size:        855    2916
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI     AhdI     AlfI     AloI    AlwNI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI     PpiI    PpuMI
    PspXI     PstI    PvuII     SacI     SapI    SexAI     SfcI     SfoI
    SgrAI     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AleI     ApaI
    ApaLI     AscI    AsiSI    AvrII     BaeI    BciVI    BfrBI     BmtI
    BseYI    BsiWI     BsmI    BsrGI    BssSI    BstBI  BstZ17I   Bsu36I
    BtgZI     ClaI    EcoRI     FseI    FspAI     HpaI     KpnI     MfeI
     MluI     NcoI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SgfI     SmaI
    SnaBI     SpeI     SrfI     SwaI     XbaI     XmaI     XmnI
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