Restriction map for pF1KB9055
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AccI        AcyI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BcgI        BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BsaHI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspHI       BspMI       
BsrBI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRV       
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        MfeI        MluI        MunI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        PacI        PflFI       PflMI       PinAI       
PmeI        PshAI       PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SgrAI       Sse8387I    SspBI       
SwaI        Tth111I     Van91I      XbaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I        399  GGTACC               G^GTACC
AccIII        459  TCCGGA               T^CCGGA
AclI         2071  AACGTT               AA^CGTT
AlwNI         605  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2263  CCTGAGG              CC^T[ACGT]AGG
AseI         2785  ATTAAT               AT^TAAT
AsnI         2785  ATTAAT               AT^TAAT
Asp718I       399  GGTACC               G^GTACC
BalI          110  TGGCCA               TGG^CCA
BanII        2406  GAGCCC               G[GA]GC[CT]^C
BbvCI        2033  CCTCAGC              CCTCAGC(-5/-2)
BclI         2802  TGATCA               T^GATCA
BglI          124  GCCGAAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1125  AGATCT               A^GATCT
BsaBI         739  GATTATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         459  TCCGGA               T^CCGGA
BsgI          448  GTGCAG               GTGCAG(16/14)
BsmBI        2374  GAGACG               CGTCTC(1/5)
BspEI         459  TCCGGA               T^CCGGA
BspLU11I      831  ACATGT               A^CATGT
BssSI        2087  CACGAG               CACGAG(-5/-1)
BstBI        2042  TTCGAA               TT^CGAA
BstEII       1995  GGTCACC              G^GT[ACGT]ACC
BstPI        1995  GGTCACC              G^GT[ACGT]ACC
BstXI        2693  CCAGCTACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2263  CCTGAGG              CC^T[ACGT]AGG
DraI          475  TTTAAA               TTT^AAA
EaeI          110  TGGCCA               [CT]^GGCC[GA]
Eco81I       2263  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1995  GGTCACC              G^GT[ACGT]ACC
EcoT22I       268  ATGCAT               ATGCA^T
FbaI         2802  TGATCA               T^GATCA
KpnI          399  GGTACC               GGTAC^C
KspI         1839  CCGCGG               CCGC^GG
MamI          739  GATTATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         110  TGGCCA               TGG^CCA
MroI          459  TCCGGA               T^CCGGA
MscI          110  TGGCCA               TGG^CCA
NdeI          410  CATATG               CA^TATG
NsiI          268  ATGCAT               ATGCA^T
NspV         2042  TTCGAA               TT^CGAA
PaeR7I         88  CTCGAG               C^TCGAG
PpuMI         854  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        2785  ATTAAT               AT^TAAT
Psp1406I     2071  AACGTT               AA^CGTT
PstI          260  CTGCAG               CTGCA^G
SacII        1839  CCGCGG               CCGC^GG
SexAI        1804  ACCTGGT              A^CC[AT]GGT
SfuI         2042  TTCGAA               TT^CGAA
SmaI         2670  CCCGGG               CCC^GGG
SnaBI        1022  TACGTA               TAC^GTA
SpeI         1881  ACTAGT               A^CTAGT
SphI         2663  GCATGC               GCATG^C
SspI          233  AATATT               AAT^ATT
StuI         2111  AGGCCT               AGG^CCT
XhoI           88  CTCGAG               C^TCGAG
XmaCI        2670  CCCGGG               C^CCGGG
XmaI         2670  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII        1840  CGCG                 CG^CG
             2217  CGCG                
AspHI        1389  GAGCAC               G[AT]GC[AT]^C
             2391  GAGCAC              
BanI          399  GGTACC               G^G[CT][GA]CC
             2208  GGTGCC              
BbrPI        1265  CACGTG               CAC^GTG
             2928  CACGTG              
BsaWI         459  TCCGGA               [AT]^CCGG[AT]
             1083  TCCGGT              
BseRI        2397  CTCCTC               GAGGAG(10/8)
               40  GAGGAG              
BsiHKAI      1389  GAGCAC               G[AT]GC[AT]^C
             2391  GAGCAC              
BstUI        1840  CGCG                 CG^CG
             2217  CGCG                
DrdI          157  GACGTGTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1952  GACCACGAAGTC        
DsaI         1839  CCGCGG               C^C[GA][CT]GG
             2000  CCGTGG              
EarI         2158  CTCTTC               CTCTTC(1/4)
             1666  GAAGAG              
FauI          952  CCCGC                CCCGC(4/6)
             1841  GCGGG               
HindIII       377  AAGCTT               A^AGCTT
             1931  AAGCTT              
Ksp632I      2158  CTCTTC               CTCTTC(1/4)
             1666  GAAGAG              
MvnI         1840  CGCG                 CG^CG
             2217  CGCG                
PmaCI        1265  CACGTG               CAC^GTG
             2928  CACGTG              
PmlI         1265  CACGTG               CAC^GTG
             2928  CACGTG              
XcmI          119  CCAAAGCCGAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2920  CCAGACCCCACGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        Asp700I     AvaI        AvaII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaAI       BsaI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI        
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