Restriction map for pF1KB9027
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BseAI       BseRI       BsiEI       
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstZ17I     CelII       
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FokI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MslI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TseI        Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII          52  CGCG                 CG^CG
AcsI          590  GAATTC               [GA]^AATT[CT]
AgeI           42  ACCGGT               A^CCGGT
AocI          664  CCTGAGG              CC^T[ACGT]AGG
ApoI          590  GAATTC               [GA]^AATT[CT]
Asp700I       423  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         469  GTGCTC               G[AT]GC[AT]^C
AvaII         513  GGACC                G^G[AT]CC
BamHI         642  GGATCC               G^GATCC
BclI          104  TGATCA               T^GATCA
BfaI          253  CTAG                 C^TAG
BmyI          469  GTGCTC               G[AGT]GC[ACT]^C
BpmI           22  CTGGAG               CTGGAG(16/14)
BsaBI         637  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          42  ACCGGT               [AT]^CCGG[AT]
BsgI           91  GTGCAG               GTGCAG(16/14)
BsiHKAI       469  GTGCTC               G[AT]GC[AT]^C
BsmI          248  GAATGC               GAATGC(1/-1)
Bsp1286I      469  GTGCTC               G[AGT]GC[ACT]^C
BspMI          29  ACCTGC               ACCTGC(4/8)
BsrFI          42  ACCGGT               [GA]^CCGG[CT]
BsrI          196  CCAGT                ACTGG(1/-1)
BstUI          52  CGCG                 CG^CG
BstXI         702  CCAGCGGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        664  CCTGAGG              CC^T[ACGT]AGG
CfoI           53  GCGC                 GCG^C
Cfr10I         42  ACCGGT               [GA]^CCGG[CT]
DraI          145  TTTAAA               TTT^AAA
DraII         449  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI          673  CGGCCA               [CT]^GGCC[GA]
Eco81I        664  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      449  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         590  GAATTC               G^AATTC
FbaI          104  TGATCA               T^GATCA
HapII          43  CCGG                 C^CGG
HgaI           71  GCGTC                GACGC(5/10)
HhaI           53  GCGC                 GCG^C
HinP1I         53  GCGC                 G^CGC
HpaII          43  CCGG                 C^CGG
MaeI          253  CTAG                 C^TAG
MaeIII        239  GTTAC                ^GT[ACGT]AC
MamI          637  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        703  CAGCGG               C[AC]G^C[GT]G
MspI           43  CCGG                 C^CGG
MvnI           52  CGCG                 CG^CG
MwoI          462  GCCCTGGGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PinAI          42  ACCGGT               A^CCGGT
PstI           31  CTGCAG               CTGCA^G
SexAI         576  ACCTGGT              A^CC[AT]GGT
SfcI           31  CTGCAG               C^T[GA][CT]AG
StuI          365  AGGCCT               AGG^CCT
TaqI          246  TCGA                 T^CGA
TspRI          63  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       246  TCGA                 T^CGA
XmnI          423  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AseI          138  ATTAAT               AT^TAAT
              168  ATTAAT              
AsnI          138  ATTAAT               AT^TAAT
              168  ATTAAT              
BstYI          35  AGATCC               [GA]^GATC[CT]
              642  GGATCC              
DdeI          348  CTTAG                C^T[ACGT]AG
              665  CTGAG               
HinfI         203  GATTC                G^A[ACGT]TC
              314  GATTC               
MflI           35  AGATCC               [GA]^GATC[CT]
              642  GGATCC              
PshBI         138  ATTAAT               AT^TAAT
              168  ATTAAT              
TfiI          203  GATTC                G^A[AT]TC
              314  GATTC               
XhoII          35  AGATCC               [GA]^GATC[CT]
              642  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BsaJI       
BssKI       BstNI       Cac8I       Cfr13I      DpnII       
EcoRII      Fnu4HI      HaeIII      HphI        ItaI        
MboI        MboII       MnlI        MseI        MvaI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       Tru9I       Tsp509I     
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