################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BfrI BglII BlnI BlpI Bpu1102I BseAI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I HindIII HpaI KpnI KspI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflIII 1886 ACGTGT A^C[GA][CT]GT Alw44I 2263 GTGCAC G^TGCAC Aor51HI 2719 AGCGCT AGC^GCT ApaLI 2263 GTGCAC G^TGCAC AviII 533 TGCGCA TGC^GCA BbeI 589 GGCGCC GGCGC^C BcgI 2271 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2945 GTATCC GTATCC(6/5) BclI 2067 TGATCA T^GATCA BfaI 2453 CTAG C^TAG BsaBI 2397 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2633 ACCGGA [AT]^CCGG[AT] Bsp1407I 1115 TGTACA T^GTACA BspHI 2492 TCATGA T^CATGA BsrBI 1429 GAGCGG CCGCTC(-3/-3) BsrGI 1115 TGTACA T^GTACA BssHII 615 GCGCGC G^CGCGC BstAPI 1130 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 1169 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2752 GACCGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2719 AGCGCT AGC^GCT EcoNI 2037 CCTAAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 2067 TGATCA T^GATCA FseI 2916 GGCCGGCC GGCCGG^CC FspI 533 TGCGCA TGC^GCA KasI 589 GGCGCC G^GCGCC MaeI 2453 CTAG C^TAG MamI 2397 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 589 GGCGCC GG^CGCC RcaI 2492 TCATGA T^CATGA SacI 322 GAGCTC GAGCT^C SalI 1949 GTCGAC G^TCGAC SapI 1476 GAAGAGC GCTCTTC(1/4) ScaI 1466 AGTACT AGT^ACT SfoI 589 GGCGCC GGC^GCC SmaI 1085 CCCGGG CCC^GGG SspBI 1115 TGTACA T^GTACA StuI 2045 AGGCCT AGG^CCT XcmI 172 CCAGAAAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1085 CCCGGG C^CCGGG XmaI 1085 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 313 GTCTAC GT^[AC][GT]AC 1949 GTCGAC AcsI 1927 GAATTT [GA]^AATT[CT] 2650 AAATTC AcyI 589 GGCGCC G[GA]^CG[CT]C 1546 GACGCC AocI 1743 CCTCAGG CC^T[ACGT]AGG 2640 CCTCAGG ApaI 895 GGGCCC GGGCC^C 1646 GGGCCC ApoI 1927 GAATTT [GA]^AATT[CT] 2650 AAATTC AspHI 322 GAGCTC G[AT]GC[AT]^C 2263 GTGCAC BalI 2 TGGCCA TGG^CCA 1985 TGGCCA BbsI 2887 GAAGAC GAAGAC(2/6) 1705 GTCTTC BbvCI 425 CCTCAGC CCTCAGC(-5/-2) 1157 CCTCAGC BglI 36 GCCGCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2416 GCCCCAGAGGC BpuAI 2887 GAAGAC GAAGAC(2/6) 1705 GTCTTC BsaAI 316 TACGTG [CT]AC^GT[GA] 460 TACGTG BsaHI 589 GGCGCC G[GA]^CG[CT]C 1546 GACGCC BseRI 432 CTCCTC GAGGAG(10/8) 1906 GAGGAG BsiEI 513 CGGCCG CG[GA][CT]^CG 1325 CGGCCG BsiHKAI 322 GAGCTC G[AT]GC[AT]^C 2263 GTGCAC BstEII 2100 GGTCACC G^GT[ACGT]ACC 2907 GGTCACC BstPI 2100 GGTCACC G^GT[ACGT]ACC 2907 GGTCACC BstYI 2396 AGATCC [GA]^GATC[CT] 2933 AGATCC Bsu36I 1743 CCTCAGG CC^T[ACGT]AGG 2640 CCTCAGG EagI 513 CGGCCG C^GGCCG 1325 CGGCCG EclXI 513 CGGCCG C^GGCCG 1325 CGGCCG Eco52I 513 CGGCCG C^GGCCG 1325 CGGCCG Eco81I 1743 CCTCAGG CC^T[ACGT]AGG 2640 CCTCAGG EcoO65I 2100 GGTCACC G^GT[ACGT]ACC 2907 GGTCACC Hin1I 589 GGCGCC G[GA]^CG[CT]C 1546 GACGCC MflI 2396 AGATCC [GA]^GATC[CT] 2933 AGATCC MluNI 2 TGGCCA TGG^CCA 1985 TGGCCA MscI 2 TGGCCA TGG^CCA 1985 TGGCCA NcoI 1140 CCATGG C^CATGG 2579 CCATGG PaeR7I 1069 CTCGAG C^TCGAG 2089 CTCGAG PpuMI 1088 GGGACCT [GA]G^G[AT]CC[CT] 1844 AGGACCC PstI 1789 CTGCAG CTGCA^G 2962 CTGCAG TfiI 216 GAATC G^A[AT]TC 1768 GATTC XhoI 1069 CTCGAG C^TCGAG 2089 CTCGAG XhoII 2396 AGATCC [GA]^GATC[CT] 2933 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I