(Circular) (Six-base) MAPSORT of: pF1KB7020.seq Check: 3448 from: 1 to: 6107 pF1KB7020 6107 bp With 182 enzymes: SgfI * June 20, 2008 10:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 835 1072 835 Size: 237 5870 AccI GT'mk_AC Cuts at: 422 2058 3145 422 Size: 1636 1087 3384 Fragments arranged by size: 3384 1636 1087 Acc65I G'GTAC_C Cuts at: 3123 3123 Size: 6107 AclI AA'CG_TT Cuts at: 5947 5947 Size: 6107 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1490 2128 3932 4364 4717 5379 1490 Size: 638 1804 432 353 662 2218 Fragments arranged by size: 2218 1804 662 638 432 353 AfeI AGC'GCT Cuts at: 2829 2829 Size: 6107 AflIII A'CryG_T Cuts at: 1994 3459 5259 5540 1994 Size: 1465 1800 281 2561 Fragments arranged by size: 2561 1800 1465 281 AleI CACnn'nnGTG Cuts at: 637 1120 637 Size: 483 5624 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1138 1925 1138 Size: 787 5320 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 421 453 421 Size: 32 6075 AlwNI CAG_nnn'CTG Cuts at: 1058 1112 1693 3087 3412 4850 1058 Size: 54 581 1394 325 1438 2315 Fragments arranged by size: 2315 1438 1394 581 325 54 ApaI G_GGCC'C Cuts at: 1007 1758 1007 Size: 751 5356 ApaLI G'TGCA_C Cuts at: 2371 4945 2371 Size: 2574 3533 ApoI r'AATT_y Cuts at: 2035 2758 3111 5460 5716 2035 Size: 723 353 2349 256 2426 Fragments arranged by size: 2426 2349 723 353 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6107 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6107 AvaI C'yCGr_G Cuts at: 775 1136 1177 1193 1401 2197 3127 775 Size: 361 41 16 208 796 930 3755 Fragments arranged by size: 3755 930 796 361 208 41 16 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1017 1050 1017 Size: 33 6074 BamHI G'GATC_C Cuts at: 3132 3132 Size: 6107 BanI G'GyrC_C Cuts at: 697 823 855 897 907 1527 3123 3537 3785 Size: 126 32 42 10 620 1596 414 248 Cuts at: 3785 3820 697 Size: 35 2984 Fragments arranged by size: 2984 1596 620 414 248 126 42 35 32 10 BanII G_rGCy'C Cuts at: 120 434 800 1007 1193 1414 1758 1795 3121 Size: 314 366 207 186 221 344 37 1326 Cuts at: 3121 4151 120 Size: 1030 2076 Fragments arranged by size: 2076 1326 1030 366 344 314 221 207 186 37 BbeI G_GCGC'C Cuts at: 701 3541 3789 701 Size: 2840 248 3019 Fragments arranged by size: 3019 2840 248 BbsI GAAGACnn'nnnn_ Cuts at: 1806 3002 1806 Size: 1196 4911 BbvCI CC'TCA_GC Cuts at: 534 1266 534 Size: 732 5375 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2368 2402 2368 Size: 34 6073 BciVI GTATCCnnnnn_n' Cuts at: 3064 4005 5061 6107 3064 Size: 941 1056 1046 3064 Fragments arranged by size: 3064 1056 1046 941 BclI T'GATC_A Cuts at: 2175 3627 2175 Size: 1452 4655 BglI GCCn_nnn'nGGC Cuts at: 150 2530 150 Size: 2380 3727 BglII A'GATC_T Cuts at: 3622 5659 3622 Size: 2037 4070 BlpI GC'TnA_GC Cuts at: 3219 3219 Size: 6107 Bme1580I G_kGCm'C Cuts at: 1007 1478 1530 1683 1758 2375 3732 3825 4949 Size: 471 52 153 75 617 1357 93 1124 Cuts at: 4949 1007 Size: 2165 Fragments arranged by size: 2165 1357 1124 617 471 153 93 75 52 BmgBI CAC'GTC Cuts at: 1995 1995 Size: 6107 BmrI ACTGGGnnnn_n' Cuts at: 797 948 2634 3319 3400 3735 5879 797 Size: 151 1686 685 81 335 2144 1025 Fragments arranged by size: 2144 1686 1025 685 335 151 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 621 819 1764 4474 621 Size: 198 945 2710 2254 Fragments arranged by size: 2710 2254 945 198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1277 1750 2476 2592 1277 Size: 473 726 116 4792 Fragments arranged by size: 4792 726 473 116 Bpu10I CC'TnA_GC Cuts at: 534 1266 1500 2146 3046 534 Size: 732 234 646 900 3595 Fragments arranged by size: 3595 900 732 646 234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 418 847 2797 3283 4162 4876 5174 418 Size: 429 1950 486 879 714 298 1351 Fragments arranged by size: 1950 1351 879 714 486 429 298 BsaI GGTCTCn'nnnn_ Cuts at: 220 484 772 1723 5811 220 Size: 264 288 951 4088 516 Fragments arranged by size: 4088 951 516 288 264 BsaAI yAC'GTr Cuts at: 426 570 3460 426 Size: 144 2890 3073 Fragments arranged by size: 3073 2890 144 BsaBI GATnn'nnATC Cuts at: 6 2509 3621 5658 6 Size: 2503 1112 2037 455 Fragments arranged by size: 2503 2037 1112 455 BsaHI Gr'CG_yC Cuts at: 698 1655 3538 3786 4488 5984 698 Size: 957 1883 248 702 1496 821 Fragments arranged by size: 1883 1496 957 821 702 248 BsaWI w'CCGG_w Cuts at: 2741 3296 3817 4495 4906 5053 5327 2741 Size: 555 521 678 411 147 274 3521 Fragments arranged by size: 3521 678 555 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 421 451 940 970 2837 2867 421 Size: 30 489 30 1867 30 3661 Fragments arranged by size: 3661 1867 489 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 531 2029 531 Size: 1498 4609 BseYI C'CCAG_C Cuts at: 1044 1314 1757 1804 2029 2729 3532 4290 4955 Size: 270 443 47 225 700 803 758 665 Cuts at: 4955 5363 1044 Size: 408 1788 Fragments arranged by size: 1788 803 758 700 665 443 408 270 225 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 433 897 1044 1098 1106 1402 1474 1651 2692 Size: 464 147 54 8 296 72 177 1041 Cuts at: 2692 3115 433 Size: 423 3425 Fragments arranged by size: 3425 1041 464 423 296 177 147 72 54 8 BsiEI CG_ry'CG Cuts at: 104 624 1436 3649 3695 4925 104 Size: 520 812 2213 46 1230 1286 Fragments arranged by size: 2213 1286 1230 812 520 46 BsiHKAI G_wGCw'C Cuts at: 434 2375 3121 3899 4089 4949 434 Size: 1941 746 778 190 860 1592 Fragments arranged by size: 1941 1592 860 778 746 190 Bsp1286I G_dGCh'C Cuts at: 120 434 800 1007 1193 1414 1478 1530 1683 Size: 314 366 207 186 221 64 52 153 Cuts at: 1683 1758 1795 2375 3121 3732 3825 3899 4089 Size: 75 37 580 746 611 93 74 190 Cuts at: 4089 4151 4949 120 Size: 62 798 1278 Fragments arranged by size: 1278 798 746 611 580 366 314 221 207 190 186 153 93 75 74 64 62 52 37 BspHI T'CATG_A Cuts at: 2600 4539 2600 Size: 1939 4168 BspMI ACCTGCnnnn'nnnn_ Cuts at: 835 1072 3008 3087 3157 3371 3673 4054 835 Size: 237 1936 79 70 214 302 381 2888 Fragments arranged by size: 2888 1936 381 302 237 214 79 70 BsrBI CCG'CTC Cuts at: 50 1539 4399 4453 5937 6102 50 Size: 1489 2860 54 1484 165 55 Fragments arranged by size: 2860 1489 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 584 812 1907 2330 2805 2819 4019 584 Size: 228 1095 423 475 14 1200 2672 Fragments arranged by size: 2672 1200 1095 475 423 228 14 BsrFI r'CCGG_y Cuts at: 205 619 925 2338 2672 3025 4105 4286 205 Size: 414 306 1413 334 353 1080 181 2026 Fragments arranged by size: 2026 1413 1080 414 353 334 306 181 BsrGI T'GTAC_A Cuts at: 1223 1223 Size: 6107 BssHII G'CGCG_C Cuts at: 723 723 Size: 6107 BssSI C'ACGA_G Cuts at: 4378 5086 4378 Size: 708 5399 BstAPI GCAn_nnn'nTGC Cuts at: 1244 3484 1244 Size: 2240 3867 BstBI TT'CG_AA Cuts at: 4468 4468 Size: 6107 BstEII G'GTnAC_C Cuts at: 2208 3015 2208 Size: 807 5300 BstXI CCAn_nnnn'nTGG Cuts at: 807 1255 2493 2922 807 Size: 448 1238 429 3992 Fragments arranged by size: 3992 1238 448 429 BstYI r'GATC_y Cuts at: 1 2504 3041 3132 3599 3622 3957 4203 4509 Size: 2503 537 91 467 23 335 246 306 Cuts at: 4509 4521 4607 5659 1 Size: 12 86 1052 449 Fragments arranged by size: 2503 1052 537 467 449 335 306 246 91 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1852 2749 1852 Size: 897 5210 BtgI C'CryG_G Cuts at: 107 842 1248 2052 2657 2687 107 Size: 735 406 804 605 30 3527 Fragments arranged by size: 3527 804 735 605 406 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 539 896 1581 1821 4057 4238 4401 539 Size: 357 685 240 2236 181 163 2245 Fragments arranged by size: 2245 2236 685 357 240 181 163 BtsI GCAGTG_nn' Cuts at: 2704 3350 2704 Size: 646 5461 DraI TTT'AAA Cuts at: 3106 3106 Size: 6107 DraIII CAC_nnn'GTG Cuts at: 1282 1282 Size: 6107 DrdI GACnn_nn'nnGTC Cuts at: 2866 3813 5157 2866 Size: 947 1344 3816 Fragments arranged by size: 3816 1344 947 EaeI y'GGCC_r Cuts at: 110 621 927 1206 1433 2073 2093 2221 3023 Size: 511 306 279 227 640 20 128 802 Cuts at: 3023 3027 3692 3866 4257 4284 5629 110 Size: 4 665 174 391 27 1345 588 Fragments arranged by size: 1345 802 665 640 588 511 391 306 279 227 174 128 27 20 4 EagI C'GGCC_G Cuts at: 621 1433 3692 621 Size: 812 2259 3036 Fragments arranged by size: 3036 2259 812 EarI CTCTTCn'nnn_ Cuts at: 218 290 508 775 1579 4130 4340 218 Size: 72 218 267 804 2551 210 1985 Fragments arranged by size: 2551 1985 804 267 218 210 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 808 4315 5057 5203 5917 808 Size: 3507 742 146 714 998 Fragments arranged by size: 3507 998 742 714 146 EcoICRI GAG'CTC Cuts at: 432 3119 432 Size: 2687 3420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1277 1490 1750 2128 2476 2592 3932 4364 4717 Size: 213 260 378 348 116 1340 432 353 Cuts at: 4717 5379 1277 Size: 662 2005 Fragments arranged by size: 2005 1340 662 432 378 353 348 260 213 116 EcoNI CCTnn'n_nnAGG Cuts at: 2149 2149 Size: 6107 EcoO109I rG'GnC_Cy Cuts at: 1003 1004 1197 1262 1754 1953 3246 1003 Size: 1 193 65 492 199 1293 3864 Fragments arranged by size: 3864 1293 492 199 193 65 1 EcoRI G'AATT_C Cuts at: 3111 3111 Size: 6107 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 510 687 2958 4126 510 Size: 177 2271 1168 2491 Fragments arranged by size: 2491 2271 1168 177 FseI GG_CCGG'CC Cuts at: 3029 3029 Size: 6107 FspI TGC'GCA Cuts at: 643 3888 643 Size: 3245 2862 HaeII r_GCGC'y Cuts at: 220 701 1332 2831 3541 3789 5019 5790 220 Size: 481 631 1499 710 248 1230 771 537 Fragments arranged by size: 1499 1230 771 710 631 537 481 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 261 293 517 549 1081 1113 1130 1162 2452 Size: 32 224 32 532 32 17 32 1290 Cuts at: 2452 2484 3949 3979 3981 4011 261 Size: 32 1465 30 2 30 2357 Fragments arranged by size: 2357 1465 1290 532 224 32 32 32 32 32 30 30 17 2 HincII GTy'rAC Cuts at: 630 1392 2059 2406 3146 630 Size: 762 667 347 740 3591 Fragments arranged by size: 3591 762 740 667 347 KasI G'GCGC_C Cuts at: 697 3537 3785 697 Size: 2840 248 3019 Fragments arranged by size: 3019 2840 248 KpnI G_GTAC'C Cuts at: 3127 3127 Size: 6107 MluI A'CGCG_T Cuts at: 5540 5540 Size: 6107 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4866 5050 4866 Size: 184 5923 MscI TGG'CCA Cuts at: 112 2095 3868 112 Size: 1983 1773 2351 Fragments arranged by size: 2351 1983 1773 MslI CAynn'nnrTG Cuts at: 637 718 1120 2920 4096 4223 637 Size: 81 402 1800 1176 127 2521 Fragments arranged by size: 2521 1800 1176 402 127 81 MspA1I CmG'CkG Cuts at: 162 174 189 417 940 1314 1635 1804 2133 Size: 12 15 228 523 374 321 169 329 Cuts at: 2133 2310 2516 3218 3532 3892 4676 4921 162 Size: 177 206 702 314 360 784 245 1348 Fragments arranged by size: 1348 784 702 523 374 360 329 321 314 245 228 206 177 169 15 12 NaeI GCC'GGC Cuts at: 207 621 2340 3027 4288 207 Size: 414 1719 687 1261 2026 Fragments arranged by size: 2026 1719 1261 687 414 NarI GG'CG_CC Cuts at: 698 3538 3786 698 Size: 2840 248 3019 Fragments arranged by size: 3019 2840 248 NcoI C'CATG_G Cuts at: 107 1248 2687 107 Size: 1141 1439 3527 Fragments arranged by size: 3527 1439 1141 NgoMIV G'CCGG_C Cuts at: 205 619 2338 3025 4286 205 Size: 414 1719 687 1261 2026 Fragments arranged by size: 2026 1719 1261 687 414 NspI r_CATG'y Cuts at: 708 2322 2844 3066 3160 3660 5263 708 Size: 1614 522 222 94 500 1603 1552 Fragments arranged by size: 1614 1603 1552 522 500 222 94 PasI CC'CwG_GG Cuts at: 973 2493 973 Size: 1520 4587 PciI A'CATG_T Cuts at: 5259 5259 Size: 6107 PflMI CCAn_nnn'nTGG Cuts at: 286 1353 2313 2516 286 Size: 1067 960 203 3877 Fragments arranged by size: 3877 1067 960 203 PfoI T'CCnGG_A Cuts at: 686 1556 1766 1914 2453 2855 3056 686 Size: 870 210 148 539 402 201 3737 Fragments arranged by size: 3737 870 539 402 210 201 148 PmeI GTTT'AAAC Cuts at: 3106 3106 Size: 6107 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 421 453 4553 4585 421 Size: 32 4100 32 1943 Fragments arranged by size: 4100 1943 32 32 PpuMI rG'GwC_Cy Cuts at: 1197 1953 1197 Size: 756 5351 PspOMI G'GGCC_C Cuts at: 1003 1754 1003 Size: 751 5356 PspXI vC'TCGA_Gb Cuts at: 1177 2197 1177 Size: 1020 5087 PstI C_TGCA'G Cuts at: 1901 3074 3154 3839 1901 Size: 1173 80 685 4169 Fragments arranged by size: 4169 1173 685 80 PvuI CG_AT'CG Cuts at: 104 104 Size: 6107 PvuII CAG'CTG Cuts at: 940 1314 1635 1804 2310 3532 3892 940 Size: 374 321 169 506 1222 360 3155 Fragments arranged by size: 3155 1222 506 374 360 321 169 RsrII CG'GwC_CG Cuts at: 4302 4302 Size: 6107 SacI G_AGCT'C Cuts at: 434 3121 434 Size: 2687 3420 SalI G'TCGA_C Cuts at: 2057 3144 2057 Size: 1087 5020 SapI GCTCTTCn'nnn_ Cuts at: 1579 4130 4340 1579 Size: 2551 210 3346 Fragments arranged by size: 3346 2551 210 SbfI CC_TGCA'GG Cuts at: 3154 3154 Size: 6107 ScaI AGT'ACT Cuts at: 1576 5624 1576 Size: 4048 2059 SexAI A'CCwGG_T Cuts at: 341 2414 2972 341 Size: 2073 558 3476 Fragments arranged by size: 3476 2073 558 SfcI C'TryA_G Cuts at: 33 1799 1897 3070 3150 3835 4803 4994 33 Size: 1766 98 1173 80 685 968 191 1146 Fragments arranged by size: 1766 1173 1146 968 685 191 98 80 SfoI GGC'GCC Cuts at: 699 3539 3787 699 Size: 2840 248 3019 Fragments arranged by size: 3019 2840 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6107 SmaI CCC'GGG Cuts at: 1195 3129 1195 Size: 1934 4173 SmlI C'TyrA_G Cuts at: 433 862 1177 2197 2812 3262 4177 4891 5153 Size: 429 315 1020 615 450 915 714 262 Cuts at: 5153 433 Size: 1387 Fragments arranged by size: 1387 1020 915 714 615 450 429 315 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6107 SphI G_CATG'C Cuts at: 3160 3660 3160 Size: 500 5607 StuI AGG'CCT Cuts at: 2155 2155 Size: 6107 StyI C'CwwG_G Cuts at: 107 635 662 944 1248 1982 2687 3241 3595 Size: 528 27 282 304 734 705 554 354 Cuts at: 3595 107 Size: 2619 Fragments arranged by size: 2619 734 705 554 528 354 304 282 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 278 895 1192 2700 2721 3690 3823 4282 4491 Size: 617 297 1508 21 969 133 459 209 Cuts at: 4491 278 Size: 1894 Fragments arranged by size: 1894 1508 969 617 459 297 209 133 21 TatI w'GTAC_w Cuts at: 1223 1574 1706 1883 1904 5622 1223 Size: 351 132 177 21 3718 1708 Fragments arranged by size: 3718 1708 351 177 132 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 940 972 2837 2869 5624 5656 940 Size: 32 1865 32 2755 32 1391 Fragments arranged by size: 2755 1865 1391 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3904 3904 Size: 6107 XbaI T'CTAG_A Cuts at: 3138 3138 Size: 6107 XcmI CCAnnnn_n'nnnnTGG Cuts at: 287 3602 287 Size: 3315 2792 XhoI C'TCGA_G Cuts at: 1177 2197 1177 Size: 1020 5087 XmaI C'CCGG_G Cuts at: 1193 3127 1193 Size: 1934 4173 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AflII AgeI AhdI AscI AvrII BfrBI BmtI BsiWI BsmI BsmBI BspEI BstZ17I ClaI CspCI EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI