Restriction map for pF1KB7011
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AclI        AflII       AgeI        AocI        AscI        
AseI        AsnI        AviII       AvrII       BamHI       
BcgI        BfrI        BglII       BlnI        BsaBI       
BsaI        BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        DrdI        EagI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        MamI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NsiI        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        SalI        SbfI        
ScaI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        2350  GACGTC               GACGT^C
Acc65I        398  GGTACC               G^GTACC
AccIII       1112  TCCGGA               T^CCGGA
Alw44I       2734  GTGCAC               G^TGCAC
Aor51HI       821  AGCGCT               AGC^GCT
ApaI           44  GGGCCC               GGGCC^C
ApaLI        2734  GTGCAC               G^TGCAC
Asp700I      1599  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       398  GGTACC               G^GTACC
AspI         1488  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         1335  GGCGCC               GGCGC^C
BbsI         2446  GAAGAC               GAAGAC(2/6)
BciVI         867  GGATAC               GTATCC(6/5)
BlpI          804  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      804  GCTGAGC              GC^T[ACGT]AGC
BpuAI        2446  GAAGAC               GAAGAC(2/6)
BseAI        1112  TCCGGA               T^CCGGA
BsmBI        2763  CGTCTC               CGTCTC(1/5)
BspEI        1112  TCCGGA               T^CCGGA
BstBI        3049  TTCGAA               TT^CGAA
CelII         804  GCTGAGC              GC^T[ACGT]AGC
CpoI         2521  CGGTCCG              CG^G[AT]CCG
DraI         2395  TTTAAA               TTT^AAA
Eco47III      821  AGCGCT               AGC^GCT
HincII        444  GTTGAC               GT[CT]^[GA]AC
HindII        444  GTTGAC               GT[CT]^[GA]AC
KasI         1335  GGCGCC               G^GCGCC
KpnI          398  GGTACC               GGTAC^C
MroI         1112  TCCGGA               T^CCGGA
NaeI         1226  GCCGGC               GCC^GGC
NarI         1335  GGCGCC               GG^CGCC
NcoI         2768  CCATGG               C^CATGG
NgoMIV       1226  GCCGGC               G^CCGGC
NruI         3094  TCGCGA               TCG^CGA
NspI         2330  GCATGT               [GA]CATG^[CT]
NspV         3049  TTCGAA               TT^CGAA
PaeR7I        247  CTCGAG               C^TCGAG
PflFI        1488  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsrII        2521  CGGTCCG              CG^G[AT]CCG
SexAI        2078  ACCAGGT              A^CC[AT]GGT
SfiI         1938  GGCCTTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1335  GGCGCC               GGC^GCC
SfuI         3049  TTCGAA               TT^CGAA
Tth111I      1488  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          247  CTCGAG               C^TCGAG
XmnI         1599  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI         1644  GTCTAC               GT^[AC][GT]AC
             3016  GTCTAC              
BbrPI        2737  CACGTG               CAC^GTG
             3103  CACGTG              
BsaWI         680  TCCGGT               [AT]^CCGG[AT]
             1112  TCCGGA              
BsrFI        1226  GCCGGC               [GA]^CCGG[CT]
             2060  ACCGGC              
BstXI        2498  CCATCATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2561  CCATTGACCTGG        
BstYI         285  GGATCT               [GA]^GATC[CT]
             1671  GGATCT              
Cfr10I       1226  GCCGGC               [GA]^CCGG[CT]
             2060  ACCGGC              
MflI          285  GGATCT               [GA]^GATC[CT]
             1671  GGATCT              
PflMI         132  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2079  CCAGGTCCTGG         
PmaCI        2737  CACGTG               CAC^GTG
             3103  CACGTG              
PmlI         2737  CACGTG               CAC^GTG
             3103  CACGTG              
PpuMI        1372  AGGACCC              [GA]G^G[AT]CC[CT]
             2081  AGGTCCT             
SacI          176  GAGCTC               GAGCT^C
             1968  GAGCTC              
SapI          958  GAAGAGC              GCTCTTC(1/4)
             2577  GAAGAGC             
Van91I        132  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2079  CCAGGTCCTGG         
XcmI          600  CCATAACCACATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2759  CCAGCGTCTCCATGG     
XhoII         285  GGATCT               [GA]^GATC[CT]
             1671  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AhdI        AluI        AlwI        AlwNI       
ApoI        AspEI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
Eam1105I    EarI        EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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