Restriction map for pF1KB7011
 (Linear) (Six-base) MAPSORT of: KIBB7011.seq  Check: 150  from: 1  to: 3135



KIBB7011.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     405    3135 
   Size:        405    2730
 
AatII G_ACGT'C

Cuts at:      0    2354    3135 
   Size:       2354     781
 
AccI GT'mk_AC

Cuts at:      0    1645    3017    3135 
   Size:       1645    1372     118

  Fragments arranged by size: 

                1645    1372     118
 
Acc65I G'GTAC_C

Cuts at:      0     398    3135 
   Size:        398    2737
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89    1687    1903    2112    2763    3009    3135 
   Size:         89    1598     216     209     651     246     126

  Fragments arranged by size: 

                1598     651     246     216     209     126      89
 
AfeI AGC'GCT

Cuts at:      0     823    3135 
   Size:        823    2312
 
AflIII A'CryG_T

Cuts at:      0     448    2738    2987    3044    3104    3135 
   Size:        448    2290     249      57      60      31

  Fragments arranged by size: 

                2290     448     249      60      57      31
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1427    2628    2661    3135 
   Size:       1427    1201      33     474

  Fragments arranged by size: 

                1427    1201     474      33
 
AleI CACnn'nnGTG

Cuts at:      0    2731    3135 
   Size:       2731     404
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1832    3135 
   Size:       1832    1303
 
AlwNI CAG_nnn'CTG

Cuts at:      0     138    2085    2135    2217    2853    3135 
   Size:        138    1947      50      82     636     282

  Fragments arranged by size: 

                1947     636     282     138      82      50
 
ApaI G_GGCC'C

Cuts at:      0      48    3135 
   Size:         48    3087
 
ApaLI G'TGCA_C

Cuts at:      0    2734    3135 
   Size:       2734     401
 
ApoI r'AATT_y

Cuts at:      0      54     352     592     743     877    1143    1758    1777 
   Size:         54     298     240     151     134     266     615      19

Cuts at:   1777    2205    2824    3135 
   Size:        428     619     311

  Fragments arranged by size: 

                 619     615     428     311     298     266     240     151
                 134      54      19
 
AvaI C'yCGr_G

Cuts at:      0     247    1275    2307    3135 
   Size:        247    1028    1032     828

  Fragments arranged by size: 

                1032    1028     828     247
 
BanI G'GyrC_C

Cuts at:      0     398    1198    1335    2460    2747    3135 
   Size:        398     800     137    1125     287     388

  Fragments arranged by size: 

                1125     800     398     388     287     137
 
BanII G_rGCy'C

Cuts at:      0      48     180     965    1282    1510    1972    2321    3135 
   Size:         48     132     785     317     228     462     349     814

  Fragments arranged by size: 

                 814     785     462     349     317     228     132      48
            
 
BbeI G_GCGC'C

Cuts at:      0    1339    3135 
   Size:       1339    1796
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2453    3135 
   Size:       2453     682
 
BbvCI CC'TCA_GC

Cuts at:      0     560    1391    2296    2818    3135 
   Size:        560     831     905     522     317

  Fragments arranged by size: 

                 905     831     560     522     317
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     861    3135 
   Size:        861    2274
 
BclI T'GATC_A

Cuts at:      0     258    1535    2192    3135 
   Size:        258    1277     657     943

  Fragments arranged by size: 

                1277     943     657     258
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1333    1945    2149    2650    3135 
   Size:       1333     612     204     501     485

  Fragments arranged by size: 

                1333     612     501     485     204
 
BlpI GC'TnA_GC

Cuts at:      0     805    3135 
   Size:        805    2330
 
Bme1580I G_kGCm'C

Cuts at:      0      48    2465    2738    3135 
   Size:         48    2417     273     397

  Fragments arranged by size: 

                2417     397     273      48
 
BmgBI CAC'GTC

Cuts at:      0     449    3015    3135 
   Size:        449    2566     120

  Fragments arranged by size: 

                2566     449     120
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      49     795    3135 
   Size:         49     746    2340

  Fragments arranged by size: 

                2340     746      49
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1406    3135 
   Size:       1406    1729
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1177    1193    1972    2002    2107    2305    2719    3135 
   Size:       1177      16     779      30     105     198     414     416

  Fragments arranged by size: 

                1177     779     416     414     198     105      30      16
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     560    1391    1504    1634    2296    2677    2818    3135 
   Size:        560     831     113     130     662     381     141     317

  Fragments arranged by size: 

                 831     662     560     381     317     141     130     113
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419    1780    2212    3135 
   Size:        419    1361     432     923

  Fragments arranged by size: 

                1361     923     432     419
 
BsaAI yAC'GTr

Cuts at:      0    2739    2988    3105    3135 
   Size:       2739     249     117      30

  Fragments arranged by size: 

                2739     249     117      30
 
BsaHI Gr'CG_yC

Cuts at:      0    1336    1721    2351    3135 
   Size:       1336     385     630     784

  Fragments arranged by size: 

                1336     784     630     385
 
BsaWI w'CCGG_w

Cuts at:      0     680    1112    3135 
   Size:        680     432    2023

  Fragments arranged by size: 

                2023     680     432
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1252    1282    1479    1509    2080    2110    2785    2815 
   Size:       1252      30     197      30     571      30     675      30

Cuts at:   2815    3135 
   Size:        320

  Fragments arranged by size: 

                1252     675     571     320     197      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1305    1366    1369    2452    3135 
   Size:       1305      61       3    1083     683

  Fragments arranged by size: 

                1305    1083     683      61       3
 
BseYI C'CCAG_C

Cuts at:      0    1376    3135 
   Size:       1376    1759
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1096    2041    2203    2983    3135 
   Size:       1096     945     162     780     152

  Fragments arranged by size: 

                1096     945     780     162     152
 
BsiHKAI G_wGCw'C

Cuts at:      0     180     536    1177    1705    1972    2738    3135 
   Size:        180     356     641     528     267     766     397

  Fragments arranged by size: 

                 766     641     528     397     356     267     180
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2769    3135 
   Size:       2769     366
 
Bsp1286I G_dGCh'C

Cuts at:      0      48     180     536     965    1177    1282    1510    1705 
   Size:         48     132     356     429     212     105     228     195

Cuts at:   1705    1972    2321    2465    2738    3135 
   Size:        267     349     144     273     397

  Fragments arranged by size: 

                 429     397     356     349     273     267     228     212
                 195     144     132     105      48
 
BspEI T'CCGG_A

Cuts at:      0    1112    3135 
   Size:       1112    2023
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     405     431    1026    2822    3135 
   Size:        405      26     595    1796     313

  Fragments arranged by size: 

                1796     595     405     313      26
 
BsrBI CCG'CTC

Cuts at:      0    1835    2031    2091    2418    3135 
   Size:       1835     196      60     327     717

  Fragments arranged by size: 

                1835     717     327     196      60
 
BsrFI r'CCGG_y

Cuts at:      0    1226    2060    3135 
   Size:       1226     834    1075

  Fragments arranged by size: 

                1226    1075     834
 
BssSI C'ACGA_G

Cuts at:      0     115     223    2364    3135 
   Size:        115     108    2141     771

  Fragments arranged by size: 

                2141     771     115     108
 
BstBI TT'CG_AA

Cuts at:      0    3050    3135 
   Size:       3050      85
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2505    2568    3135 
   Size:       2505      63     567

  Fragments arranged by size: 

                2505     567      63
 
BstYI r'GATC_y

Cuts at:      0     285    1671    3135 
   Size:        285    1386    1464

  Fragments arranged by size: 

                1464    1386     285
 
BtgI C'CryG_G

Cuts at:      0    1301    1340    2181    2403    2648    2718    2768    3135 
   Size:       1301      39     841     222     245      70      50     367

  Fragments arranged by size: 

                1301     841     367     245     222      70      50      39
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1886    2678    2769    3135 
   Size:       1886     792      91     366

  Fragments arranged by size: 

                1886     792     366      91
 
BtsI GCAGTG_nn'

Cuts at:      0    2053    2466    2795    3081    3135 
   Size:       2053     413     329     286      54

  Fragments arranged by size: 

                2053     413     329     286      54
 
DraI TTT'AAA

Cuts at:      0    2397    3135 
   Size:       2397     738
 
DraIII CAC_nnn'GTG

Cuts at:      0     278     489    1691    3135 
   Size:        278     211    1202    1444

  Fragments arranged by size: 

                1444    1202     278     211
 
EaeI y'GGCC_r

Cuts at:      0     692     725     841     887    2050    2507    2558    2606 
   Size:        692      33     116      46    1163     457      51      48

Cuts at:   2606    3135 
   Size:        529

  Fragments arranged by size: 

                1163     692     529     457     116      51      48      46
                  33
 
EarI CTCTTCn'nnn_

Cuts at:      0     953    1343    1594    2572    3135 
   Size:        953     390     251     978     563

  Fragments arranged by size: 

                 978     953     563     390     251
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     350    1242    1333    2587    3046    3135 
   Size:        350     892      91    1254     459      89

  Fragments arranged by size: 

                1254     892     459     350      91      89
 
EcoICRI GAG'CTC

Cuts at:      0     178    1970    3135 
   Size:        178    1792    1165

  Fragments arranged by size: 

                1792    1165     178
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89    1177    1193    1687    1903    1972    2002    2107 
   Size:         89    1088      16     494     216      69      30     105

Cuts at:   2107    2112    2305    2719    2763    3009    3135 
   Size:          5     193     414      44     246     126

  Fragments arranged by size: 

                1088     494     414     246     216     193     126     105
                  89      69      44      30      16       5
 
EcoO109I rG'GnC_Cy

Cuts at:      0      44      45    1373    1947    2082    2709    3135 
   Size:         44       1    1328     574     135     627     426

  Fragments arranged by size: 

                1328     627     574     426     135      44       1
 
EcoRI G'AATT_C

Cuts at:      0     743     877    1143    3135 
   Size:        743     134     266    1992

  Fragments arranged by size: 

                1992     743     266     134
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     444     825    3018    3135 
   Size:        444     381    2193     117

  Fragments arranged by size: 

                2193     444     381     117
 
HaeII r_GCGC'y

Cuts at:      0     825    1339    2684    2706    3135 
   Size:        825     514    1345      22     429

  Fragments arranged by size: 

                1345     825     514     429      22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     642     674     876     908    1252    1284    1413    1445 
   Size:        642      32     202      32     344      32     129      32

Cuts at:   1445    1479    1511    2614    2646    2647    2679    3135 
   Size:         34      32    1103      32       1      32     456

  Fragments arranged by size: 

                1103     642     456     344     202     129      34      32
                  32      32      32      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0     446    3135 
   Size:        446    2689
 
KasI G'GCGC_C

Cuts at:      0    1335    3135 
   Size:       1335    1800
 
KpnI G_GTAC'C

Cuts at:      0     402    3135 
   Size:        402    2733
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1003    3135 
   Size:       1003    2132
 
MscI TGG'CCA

Cuts at:      0     889    2560    2608    3135 
   Size:        889    1671      48     527

  Fragments arranged by size: 

                1671     889     527      48
 
MslI CAynn'nnrTG

Cuts at:      0    2164    2503    2554    2731    2788    3135 
   Size:       2164     339      51     177      57     347

  Fragments arranged by size: 

                2164     347     339     177      57      51
 
MspA1I CmG'CkG

Cuts at:      0    1303    1545    2490    2650    2720    3135 
   Size:       1303     242     945     160      70     415

  Fragments arranged by size: 

                1303     945     415     242     160      70
 
NaeI GCC'GGC

Cuts at:      0    1228    3135 
   Size:       1228    1907
 
NarI GG'CG_CC

Cuts at:      0    1336    3135 
   Size:       1336    1799
 
NcoI C'CATG_G

Cuts at:      0    2768    3135 
   Size:       2768     367
 
NgoMIV G'CCGG_C

Cuts at:      0    1226    3135 
   Size:       1226    1909
 
NruI TCG'CGA

Cuts at:      0    3096    3135 
   Size:       3096      39
 
NspI r_CATG'y

Cuts at:      0    2334    3135 
   Size:       2334     801
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     138    2085    3135 
   Size:        138    1947    1050

  Fragments arranged by size: 

                1947    1050     138
 
PfoI T'CCnGG_A

Cuts at:      0    2084    2265    3135 
   Size:       2084     181     870

  Fragments arranged by size: 

                2084     870     181
 
PmlI CAC'GTG

Cuts at:      0    2739    3105    3135 
   Size:       2739     366      30

  Fragments arranged by size: 

                2739     366      30
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3031    3063    3135 
   Size:       3031      32      72

  Fragments arranged by size: 

                3031      72      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1373    2082    3135 
   Size:       1373     709    1053

  Fragments arranged by size: 

                1373    1053     709
 
PspOMI G'GGCC_C

Cuts at:      0      44    3135 
   Size:         44    3091
 
PspXI vC'TCGA_Gb

Cuts at:      0     247    3135 
   Size:        247    2888
 
PstI C_TGCA'G

Cuts at:      0     416    1996    2012    3135 
   Size:        416    1580      16    1123

  Fragments arranged by size: 

                1580    1123     416      16
 
RsrII CG'GwC_CG

Cuts at:      0    2522    3135 
   Size:       2522     613
 
SacI G_AGCT'C

Cuts at:      0     180    1972    3135 
   Size:        180    1792    1163

  Fragments arranged by size: 

                1792    1163     180
 
SacII CC_GC'GG

Cuts at:      0    1304    2651    2721    3135 
   Size:       1304    1347      70     414

  Fragments arranged by size: 

                1347    1304     414      70
 
SapI GCTCTTCn'nnn_

Cuts at:      0     953    2572    3135 
   Size:        953    1619     563

  Fragments arranged by size: 

                1619     953     563
 
SexAI A'CCwGG_T

Cuts at:      0    2078    3135 
   Size:       2078    1057
 
SfcI C'TryA_G

Cuts at:      0     412    1133    1992    2008    3135 
   Size:        412     721     859      16    1127

  Fragments arranged by size: 

                1127     859     721     412      16
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1945    3135 
   Size:       1945    1190
 
SfoI GGC'GCC

Cuts at:      0    1337    3135 
   Size:       1337    1798
 
SmlI C'TyrA_G

Cuts at:      0     247     434    1795    2227    3135 
   Size:        247     187    1361     432     908

  Fragments arranged by size: 

                1361     908     432     247     187
 
StuI AGG'CCT

Cuts at:      0    1525    1561    1939    3135 
   Size:       1525      36     378    1196

  Fragments arranged by size: 

                1525    1196     378      36
 
StyI C'CwwG_G

Cuts at:      0      71     521     695     791     872    1940    2768    3135 
   Size:         71     450     174      96      81    1068     828     367

  Fragments arranged by size: 

                1068     828     450     367     174      96      81      71
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     163    2510    2847    3135 
   Size:        163    2347     337     288

  Fragments arranged by size: 

                2347     337     288     163
 
TatI w'GTAC_w

Cuts at:      0    3041    3135 
   Size:       3041      94
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     101     133     734     766     909     941    2080    2112 
   Size:        101      32     601      32     143      32    1139      32

Cuts at:   2112    2992    3024    3135 
   Size:        880      32     111

  Fragments arranged by size: 

                1139     880     601     143     111     101      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1491    3135 
   Size:       1491    1644
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     607    2766    3135 
   Size:        607    2159     369

  Fragments arranged by size: 

                2159     607     369
 
XhoI C'TCGA_G

Cuts at:      0     247    3135 
   Size:        247    2888
 
XmnI GAAnn'nnTTC

Cuts at:      0    1603    3135 
   Size:       1603    1532
 
ZraI GAC'GTC

Cuts at:      0    2352    3135 
   Size:       2352     783
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BssSI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NruI     NspI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PstI    RsrII     SacI    SacII
     SapI    SexAI     SfcI     SfiI     SfoI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AloI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BfrBI    BglII     BmtI     BsaI    BsaBI
    BsiEI    BsiWI     BsmI    BspHI    BsrDI    BsrGI   BssHII   BstAPI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DrdI     EagI    EcoNI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     MfeI     MluI
     NdeI     NheI     NotI     NsiI     PacI     PasI     PciI     PmeI
    PshAI     PsiI     PsrI     PvuI    PvuII     SalI    SanDI     SbfI
     ScaI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XmaI
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